AbstractObservational evidence has shown that the Earth's tropics have widened since 1980. However, climate models underestimate the observed tropical expansion rate, with a large spread among individual models. The proposal of internal variability to account for model–observation differences is hindered by the limited availability of sufficient realizations from models in the Coupled Model Intercomparison Project (CMIP), restricting the accuracy of quantitative contribution estimation. The emergence of a single model initial‐condition large ensemble provides a new opportunity to quantify the role of internal variability. Here, using large‐ensemble simulations from two individual models complemented with CMIP Phase 6 (CMIP6) simulations, we show evidence that the recent widening of the tropics is mainly caused by internal variability related to the Interdecadal Pacific Oscillation (IPO). The positive‐to‐negative phase transition of the IPO from 1980 to 2014 reduced the meridional tropospheric temperature gradient, resulting in poleward shifts in tropical edges. After adjusting the IPO trends simulated by individual realizations to ensure consistency with the observations, the IPO phase transition can account for at least 73% (66%) of the observed tropical expansion rate in the Northern Hemisphere based on the metric of the meridional stream function (surface zonal wind). The IPO is also essential for shaping tropical expansion‐related precipitation changes. Our results underscore the significance of considering internal variability when explaining model–observation differences and understanding intermodel uncertainty.