Differential Phase Research Articles

Atomic-scale revealing defects in ion irradiated 4H-SiC

Here we identified atomic-scale defects in ion irradiated 4H-SiC, by integrated differential phase contrast technique equipped on aberration-corrected transmission electron microscopy and Raman scattering spectrometer. In 21 MeV Ni+ ions irradiated 4H-SiC, in which electronic energy loss is dominant almost at the whole penetration depth, Si- and C-related vacancies and interstitials are successfully imaged. In 900 keV Si+ ions irradiated 4H-SiC, however, the electronic energy loss plays the key role near the surface, and the nuclear and electronic energy loss are comparable at the depth of the peak damaged zone. As a result, 900 keV Si+ ions produce more point defects, atomic-scale in-plane and out-of-plane lattice distortions, and plenty of dislocations at the depth of damage peak. Meantime, these imaged point defects may provide direct detailed information for the vibration modes of defects in the Raman spectrum of the damaged 4H-SiC. This study illustrates the compelling intrinsic nature of point defects induced by energetic ions, providing important fundamental information for nuclear and electronic applications.

Parabolic gratings enhance the X-ray sensitivity of Talbot interferograms

In grating-based X-ray Talbot interferometry, the wave nature of X-ray radiation is exploited to generate phase contrast images of objects that do not generate sufficient contrast in conventional X-ray imaging relying on X-ray absorption. The phase sensitivity of this interferometric technique is proportional to the interferometer length and inversely proportional to the period of gratings. However, the limited spatial coherency of X-rays limits the maximum interferometer length, and the ability to obtain smaller-period gratings is limited by the manufacturing process. Here, we propose a new optical configuration that employs a combination of a converging parabolic micro-lens array and a diverging micro-lens array, instead of a binary phase grating. Without changing the grating period or the interferometer length, the phase signal is enhanced because the beam deflection by a sample is amplified through the array of converging-diverging micro-lens pairs. We demonstrate that the differential phase signal detected by our proposed set-up is twice that of a Talbot interferometer, using the same binary absorption grating, and with the same overall inter-grating distance.

Single-shot quantitative differential phase contrast imaging combined with programmable polarization multiplexing illumination.

We propose a single-shot quantitative differential phase contrast method with polarization multiplexing illumination. In the illumination module of our system, a programmable LED array is divided into four quadrants and covered with polarizing films of four different polarization angles. We use a polarization camera with polarizers before the pixels in the imaging module. By matching the polarization angle between the polarizing films over the custom LED array and the polarizers in the camera, two sets of asymmetric illumination acquisition images can be calculated from a single-shot acquisition image. Combined with the phase transfer function, we can calculate the quantitative phase of the sample. We present the design, implementation, and experimental image data demonstrating the ability of our method to obtain quantitative phase images of a phase resolution target, as well as Hela cells.

Atomically Unraveling the Structural Evolution of Surfaces and Interfaces in Metal Halide Perovskite Quantum Dots.

Revealing the local structural change of metal halide perovskites (MHPs) induced by external conditions is important to understand its performance and stability in optoelectronic applications. However, previous studies on the properties and structures of MHPs are usually limited by the spatial resolution of the probe, and it is still challenging to obtain its atomic structural information in real space. In this work, the integrated differential-phase-contrast scanning transmission electron microscopy is applied to the low-dose imaging of CsPbI3 quantum dots (QDs). In particular, the local structures in QDs, such as surfaces and interfaces, can be atomically resolved. Then, the structural evolution of CsPbI3 QDs under various external conditions can be unraveled during in situ heating or ex situ treatments, where it lose cubic shapes and fuse to larger particles. The changes in surfaces and interfaces with missing Cs ions and PbI6 octahedrons can be semi-quantitatively studied by profile analysis and bond-length measurement in images. Finally, density functional theory calculations are performed to illustrate the properties and stabilities of the different structures that are observed. These results provide atomic-scale insights into the structural evolution of QDs, which is of great importance to modify the performance of perovskite materials and devices.

Transcriptome changes during osteogenesis of porcine mesenchymal stem cells derived from different types of synovial membranes and genetic background

Synovial membrane mesenchymal stem cells (SMSCs) often serve as in vitro model for bone disease, but the molecular mechanisms driving osteogenesis in SMSCs from different donor cells of various sources and breeds remain unclear. In this study, porcine SMSCs isolated from adipose synovium (FP) and fibrous synovium (FS) of Angeln Saddleback (AS) and German Landrace (DL) were used to discover the signaling network change after osteogenic induction. During osteogenic differentiation, mineral deposition was first observed at day 14 and further increased until day 21. Transcriptional changes between day 1 and day 21 were enriched in several signaling pathways, including Wnt, PI3K-Akt, and TGF-beta pathway. Certain pathways related to osteogenesis, including osteoblast differentiation, regulation of bone mineralization, and BMP signaling pathway, were enriched at late time points, as confirmed by the osteogenic markers ALPL, COL1A1, and NANOG. A fraction of differentially expressed genes (DEGs) were found between FP and FS, while DEGs between AS and DL increased during the differentiation phase until day 7 and then decreased from day 14 to day 21. These genes are involved in several important signaling pathways, including TGF-beta, Wnt, and lipid-related signaling pathways, suggesting that SMSCs from these two breeds have different osteogenic capabilities.

PHASE RELATIONS IN THE Ag8SiS6–Ag8SiTe6 SYSTEM AND CHARACTERIZATION OF SOLID SOLUTIONS

Argyrodite family compounds and phases based on them are valuable ecologically friendly functional materials that exhibit a number of functional propreties, such as thermoelectric, photoelectric, optical, and other. On the other hand, having Cu+ and Ag+ ion conductivity, they are ionic conductors, and can be used as electrochemical sensors, electrodes, or electrolyte materials in solid-state batteries, displays, etc. In the present paper, phase relations in the Ag8SiS6–Ag8SiTe6 system were studied by differential thermal analysis and X-ray diffraction phase techniques and a T-x phase diagram was constructed. It is established that the system is quasi-binary and is characterized by the formation of a continuous series of substitutional solid solutions between Ag8SiTe6 and HT- Ag8SiS6 compounds. With the formation of solid solutions, the temperature of the polymorphic transition of the Ag8SiS6 decreases. This leads to the stabilization of the ion-conducting cubic phase in the range of compositions 30 mol. % Ag8SiTe6 at room temperature and below. The homogeneity regions based on RT-Ag8SiS6 are 10 mol. %. According to XRD data, the crystal lattice parameters of the obtained solid solutions were calculated and a their linear dependence on the composition is shown

One kilometer balanced analog photonic link based on a single multicore fiber

A one kilometer balanced microwave photonic link based on a single four core multicore optical fiber is demonstrated for the first time, to the best of our knowledge. The radio frequency gains and differential phase stabilities over temperature were measured and compared to a conventional balanced link based on two spans of single mode fiber. Both the multicore fiber link and the single mode fiber balanced link exhibited differential phase fluctuations of |Δφ|≤13° at 25 GHz (corresponding to a differential delay or skew, |Δt|, of ≤1.4ps) under identical periodic temperature oscillations of ±13°C over a 60 min period. In contrast, previous studies have shown that two single mode fiber spans should have exhibited up to an order of magnitude greater Δφs compared to the multicore fiber. Our different results can be attributed to how the fibers are arranged; the previous works kept the two single mode fiber spans separate, on different spools, whereas in this work the two single mode fiber spans were co-located as close as possible together by spooling them ‘side-by-side’ onto a single mandrel, more representative of a balanced link in application. In addition, the radio-frequency crosstalk between the multicore fiber’s cores were measured, exhibiting acceptable levels at least 60 dB below the signal for all core combinations. The crosstalk is shown to be dominated by occurrences at localized points, e.g. at the multiplexers (fan-in/fan-outs) and splices, rather than during propagation. Regardless of the single mode fiber link’s performance, the measurements here demonstrate that multicore fiber-based balanced links are a viable single fiber alternative to conventional dual single mode fiber-based balanced links.

Simultaneous OSNR and chromatic dispersion monitoring based on optical tones power ratio

A novel technique is proposed for simultaneous monitoring of optical signal-to-noise ratio (OSNR) and chromatic dispersion (CD). The scheme is based on the cross-phase modulation effect in a highly nonlinear fiber, which induces a change in the optical tones according to the impairments. The power ratio of the optical tones at two separate frequencies is utilized for monitoring. Improvements in the maximum measurable OSNR, CD and dynamic range can be obtained using only the clock tone for the non-return-to-zero, return-to-zero with a 50% duty cycle differential quadrature phase-shift keying, differential phase-shift keying and on-off keying signals at 80 Gb/s. For the return to zero differential quadrature phase shift keying (RZ-DQPSK) system, the OSNR of 4–40 dB can be monitored with a 36.73 dB dynamic range, and a chromatic dispersion of 0–117 ps/nm can be monitored with a 48.68 dB dynamic range. A correlation is built to describe the relationship of the power ratio with the OSNR and chromatic dispersion which has a root-mean-squared error of 1.41. The delay group dispersion insensitivity, the impact of the input signal power, and the filter selection are investigated. The design enables accurate monitoring, simple operation and may be used in a wide range.

Highly sensitive plasmonic biosensor enhanced by perovskite-graphene hybrid configuration

This study theoretically proposes a highly sensitive plasmonic biosensor comprising a silver film, perovskite nanosheets, bi-layer silicon nanosheets, and a graphene overlayer. The introduction of perovskite-graphene hybrid structure can reduce the optical reflectivity of the proposed biosensor configuration so that photons are converted into energy to support surface plasmon resonance (SPR) enhancement. Unlike conventional angular modulation, our proposed biosensor configuration is designed using phase modulation, offering sensitivity enhancements as high as 3 orders of magnitude. Excellent biosensing performances have been examined by optimizing the silver film thickness and the number of perovskite nanosheets. Considering a tiny refractive index change of 0.0012 refractive index unit (RIU) caused by biomolecules adsorption behavior, the optimal plasmonic biosensor configuration of 46-nm silver film–5.7-nm silicon interlayer-1-layer CH3NH3PbI3 perovskite film–0.7-nm silicon interlayer-1-layer graphene can produce the highest detection sensitivity of 1.2314 × 105 degree/RIU. In addition, an excellent linear response in the differential phase can be obtained, as the refractive index variation of the sensing interface can be as low as 10−6 RIU, showing great potential in performing ultrasensitive biosensing.

ETV2 primes hematoendothelial gene enhancers prior to hematoendothelial fate commitment

Mechanisms underlying distinct specification, commitment, and differentiation phases of cell fate determination remain undefined due to difficulties capturing these processes. Here, we interrogate the activity of ETV2, a transcription factor necessary and sufficient for hematoendothelial differentiation, within isolated fate intermediates. We observe transcriptional upregulation of Etv2 and opening of ETV2-binding sites, indicating new ETV2 binding, in a common cardiac-hematoendothelial progenitor population. Accessible ETV2-binding sites are active at the Etv2 locus but not at other hematoendothelial regulator genes. Hematoendothelial commitment coincides with the activation of a small repertoire of previously accessible ETV2-binding sites at hematoendothelial regulators. Hematoendothelial differentiation accompanies activation of a large repertoire of new ETV2-binding sites and upregulation of hematopoietic and endothelial gene regulatory networks. This work distinguishes specification, commitment, and sublineage differentiation phases of ETV2-dependent transcription and suggests that the shift from ETV2 binding to ETV2-bound enhancer activation, not ETV2 binding to target enhancers, drives hematoendothelial fate commitment.

A Novel Scheme for AC Microgrid Protection Based on Differential Phase Angle of Fault Component Current

A Novel Scheme for AC Microgrid Protection Based on Differential Phase Angle of Fault Component Current

Study on Differential Phase Delay of Same‐Beam VLBI in Rendezvous and Docking of Chang'E‐5

AbstractThe Chang'E‐5 (CE5) ascender achieved lunar orbit autonomous rendezvous and docking with orbiter‐returner successfully on 5 December 2020. Same‐beam Very Long Baseline Interferometry (VLBI) (SBI) observations were performed for the first time for autonomous rendezvous and docking in lunar orbit. In this paper, by studying the CE5 rendezvous and docking data observed by the Chinese VLBI network and analyzing the residual differential phase delay of the autonomous control phase, we successfully captured the maneuver of the 5 km holding of the orbiter‐returner. The differential phase delay (DPD) data of the far‐range guidance phase were used to perform orbit determination of the ascender and orbiter‐returner successfully. The results of orbit determination show that the DPD data can improve the relative position accuracy between the ascender and the orbiter‐returner, and the residual root mean square of the DPD data is 5 ps. Furthermore, when one of the probes has only DPD data, its orbit can also be determined.

Front Cover

A reflectional quantitative differential phase microscopy (RQDPM) based on polarized wavefront phase modulation and partially coherent full‐aperture illumination is introduced for imaging thick and opaque samples. RQDPM utilizes the polarization‐dependent characteristic of a phase‐type spatial light odulator to divide the object wave into two identical copies with a certain shear distance. When loading different modulation images to the spatial light modulator, phase derivative images with different shearing directions and phase shifts can be obtained. Finally, the phase distribution of the sample is obtained.For further details please visit the article by Ying Ma, Taiqiang Dai, Lan Yu, Lin Ma, Sha An, Yang Wang, Min Liu, Juanjuan Zheng, Liang Kong, Chao Zuo, and Peng Gao (e202200325).image

Comparison of Cloud Structures of Storms Producing Lightning at Different Distance Based on Five Years Measurements of a Doppler Polarimetric Vertical Cloud Profiler

We processed five years of measurements (2018–2022) of a vertically pointing radar MIRA 35c at the Milešovka meteorological observatory with the aim of analyzing the cloud structure of thunderstorms and comparing differences in measured data for cases when lightning discharges were observed very close to the radar position, and for cases when lightning discharges were observed at a greater distance from the radar position. The MIRA 35c radar is a Doppler polarimetric radar working at 35 GHz (Ka-band) with a vertical resolution of 28.9 m and a time resolution of approximately 2 s. For the analysis, we considered radar data whose radar reflectivity was at least 10 dBZ at 5 km or higher above the radar to ensure that there was a cloud above the radar. We divided the radar data into “near” data (a lightning discharge was registered up to 1 km from the radar position) and “far” data (a lightning discharge was registered from 7.5 to 10 km from the radar position). We compared the following quantities: (i) Power in co-channel (pow), (ii) power in cross-channel (pow-cx), (iii) phase in co-channel (pha), (iv) phase in cross-channel (pha-cx), (v) equivalent radar reflectivity (Ze), (vi) Linear Depolarization Ratio (LDR), (vii) co-polar correlation coefficient (RHO), (viii) Doppler radial velocity (V), (ix) Doppler spectrum width (RMS), and (x) Differential phase (Phi). Pow, pow-cx, pha, pha-cx, and V are basic data measured by the radar, while Ze, LDR, RHO, RMS, and Phi are derived quantities. Our results showed that the characteristics of the compared radar quantities are clearly distinct for “near” dataset from “far” dataset. Furthermore, we found out that there is a clear evolution close to the time of discharges of the observed radar quantities in the “near” dataset, which is not that obvious in the “far” dataset.

Mesomorphic Investigation of Binary Mixtures of Liquid Crystal Molecules with Different Mesogenic Architectonics

Different binary phase diagrams, made from two differently substituted three-rings azo/ester and azomethine/ester compounds of the same terminal alkoxy side chain of six carbons, as opposed to the other terminal polar substituent, which can either donate electrons or withdraw electrons including H. The thermal behavior of the prepared derivatives was investigated by differential scanning calorimetry and phases identified by polarized optical microscope. The first group of the binary mixtures was made from laterally F-substituted azo/ester derivatives and their laterally neat analogues. The second group of binary mixtures was made from laterally methoxy-substituted azomethine/ester derivatives and their laterally neat analogues. The final type of investigated phase diagrams was made from the laterally substituted azo and azomethine components bearing different lateral polar groups and different mesogenic moieties. Results were reviewed using phase diagrams that were produced and it was found that different mesomorphic characteristics were seen to depend on the mesogenic component as well as lateral and terminal polar groups. In all cases, these mixtures have been determined to have low melting-temperature eutectic compositions, while linear or negative deviation of nematic or smectic isotropic composition temperature dependence was observed.

Self-supervised neural network for phase retrieval in QDPC microscopy.

Quantitative differential phase contrast (QDPC) microscope plays an important role in biomedical research since it can provide high-resolution images and quantitative phase information for thin transparent objects without staining. With weak phase assumption, the retrieval of phase information in QDPC can be treated as a linearly inverse problem which can be solved by Tikhonov regularization. However, the weak phase assumption is limited to thin objects, and tuning the regularization parameter manually is inconvenient. A self-supervised learning method based on deep image prior (DIP) is proposed to retrieve phase information from intensity measurements. The DIP model that takes intensity measurements as input is trained to output phase image. To achieve this goal, a physical layer that synthesizes the intensity measurements from the predicted phase is used. By minimizing the difference between the measured and predicted intensities, the trained DIP model is expected to reconstruct the phase image from its intensity measurements. To evaluate the performance of the proposed method, we conducted two phantom studies and reconstructed the micro-lens array and standard phase targets with different phase values. In the experimental results, the deviation of the reconstructed phase values obtained from the proposed method was less than 10% of the theoretical values. Our results show the feasibility of the proposed methods to predict quantitative phase with high accuracy, and no use of ground truth phase.

Classification of common variable immunodeficiency through immunological and clinical phenotyping in Moroccan patients.

Objective: Common variable immunodeficiency (CVID) is a complex inborn error of humoral immunity with complications of both infectious and non-infectious origins. Classifications of CVID patients provide a clearer understanding of the pathogenesis, prediction, and management of non-infectious complications. This study aims to classify Moroccan CVID patients based on the European classification (EUROclass). Materials and Methods: We recruited 20 CVID patients meeting standard diagnostic criteria (5-6). After collecting clinical and demographic data, we used flow cytometry to analyze B-cell subsets and group patients and assess the relation of each group with clinical manifestations. Results: 90% of the patients in our cohort study had a history of respiratory infections. The noninfectious manifestations included splenomegaly, autoimmunity, lymphadenopathy, and granulomatous diseases diagnosed in 50%, 45%, 40%, and 25% of patients, respectively. We observed significant co-occurrence of splenomegaly with autoimmunity and granulomatous diseases to a lesser extent. Patients had a significant reduction in total, switched memory, marginal zone-like, plasma blasts, and a substantial increase in the percentage of activated B cells, suggesting a defect in the late phases of B-cell differentiation. This condition was linked with an increased occurrence of splenomegaly and granulomatous affections. Besides, patients also had an expansion of CD21low B-cells, which was strongly associated with splenomegaly. Conclusion: The classification of the first Moroccan cohort of CVID patients showed agreement with previous results. It suggests the possibility of adopting this approach on a global scale for better diagnosis and follow-up of CVID patients.

A novel code shift keying for label superimposing in optical label switching

Optical label switching (OLS) is an attractive solution for the packet-based optical switching. A novel modulation method, i. e., code shift keying (CSK) is proposed for label modulation in this paper. The payload signal applies differential phase shift keying (DPSK) in Manchester code or NRZ format when the label data is “1” or “0”, respectively. The payload signal is detected by a Mach-Zehnder delay interferometer (MZDI) with two-bit delay difference between the two arms. The label signal is detected by another MZDI with a-half-bit delay difference between the two arms. The proposed CSK based OLS significantly increases the code efficiency of the payload signal without sacrificing the extinction ratio (ER). The feasibility of the CSK based OLS is verified by the simulation.

Microphysical Characteristics of Raindrop Size Distribution and Implications for Dual-Polarization Radar Quantitative Precipitation Estimations in the Tianshan Mountains, China

In order to improve the understanding of the microphysical characteristics of raindrop size distribution (DSD) under different rainfall rates (R) classes, and broaden the knowledge of the impact of radar wavelengths and R classes on the QPE of dual-polarization radars in the Tianshan Mountains, a typical arid area in China, we investigated the microphysical characteristics of DSD across R classes and dual-polarimetric radar QPE relationships across radar wavelengths and R classes, based on the DSD data from a PARSIVEL2 disdrometer at Zhaosu in the Tianshan Mountains during the summers of 2020 and 2021. As the R class increased, the DSD became wider and flatter. The mean value of the mass-weighted mean diameters (Dm) increased, while the mean value of logarithm normalized intercept parameters (log10 Nw) decreased after increasing from C1 to C3, as the R class increased. The largest contributions to R and the radar reflectivity factor from large raindrops (diameter > 3 mm) accounted for approximately 50% and 97%, respectively, while 84% of the total raindrops were small raindrops (diameter < 1 mm). Dual-polarization radars—horizontal polarization reflectivity (Zh), differential reflectivity (Zdr), and specific differential phase (Kdp)—were retrieved based on the DSD data using the T-matrix scattering method. The DSD-based polarimetric radar QPE relations of a single-parameter (R(Zh), R(Kdp)), and double-parameters (R(Zh,Zdr), R(Kdp,Zdr)) on the S-, C-, and X-bands were derived and evaluated. Overall, the performance of the R(Kdp) (R(Kdp,Zdr)) scheme was better than that of R(Zh) (R(Zh,Zdr)) for the QPE in the three bands. Furthermore, we have for the first time confirmed and quantified the performance differences in the QPE relationship of dual-polarization radars under different schemes, radar wavelengths, and R classes in typical arid areas of China. Therefore, selecting an appropriate dual-polarization radar band and QPE scheme for different R classes is necessary to improve the QPE ability compared with an independent scheme under all R classes.

Hollow Psychography: Toward Simultaneous 4D Scanning Transmission Electron Microscopy and Electron Energy Loss Spectroscopy.

With the recent development of high-acquisition-speed pixelated detectors, 4D scanning transmission electron microscopy (4D-STEM) is becoming routinely available in high-resolution electron microscopy. 4D-STEM acts as a "universal" method that provides local information on materials that is challenging to extract from bulk techniques. It extends conventional STEM imaging to include super-resolution techniques and to provide quantitative phase-based information, such as differential phase contrast, psychography, or Bloch wave phase retrieval. However, an important missing factor is the chemical and bonding information provided by electron energy loss spectroscopy (EELS). 4D-STEM and EELS cannot currently be acquired simultaneously due to the overlapping geometry of the detectors. Here, the feasibility of modifying the detector geometry to overcome this challenge for bulk specimens is demonstrated, and the use of a partial or defective detector for ptycholgaphic structural imaging is explored. Results show that structural information beyond the diffraction-limit and chemical information from the material can be extracted together, resulting in simultaneous multi-modal measurements, adding the additional dimensions of spectral information to 4D datasets.