Measurements of carrier frequency phase differences of global navigation satellite system signals between multiple antennas are used in many coordinate and navigation applications. Global navigation satellite systems signal simulators are used for controlling global navigation satellite systems receiver’s instrumental errors. Therefore, it is necessary to determine the systematic error of the carrier frequency phase of generated signals for signal simulators. For this purpose a new calibration method of global navigation satellite systems simulators was developed. Method is based on refined errors model of generated phase differences of two radio frequency channels of the simulator. The proposed method was implemented using a wideband oscilloscope as an analog-to-digital converter. Approbation of the calibration method was carried out by comparison with the measurement results obtained using the State secondary standard of units of complex transmission coefficients in the range of 0...–60 dB and complex reflection coefficients in the range of 0.002–1 in the frequency range of 0.05–65 GHz (registration No. 2.1.ZZT.0210.2015). The calibration method allows one to determine carrier frequencies phase offsets of navigation signals for global navigation satellite systems simulators with expanded uncertainty (with a coverage factor of 3) of 1°. The proposed method provides the required accuracy of generating phase differences of the carrier frequencies of global navigation satellite systems signals is ensured for modern types of global navigation satellite systems simulators.