Petiole Color Research Articles

Spinach genomes reveal migration history and candidate genes for important crop traits.

Spinach (Spinacia oleracea) is an important leafy crop possessing notable economic value and health benefits. Current genomic resources include reference genomes and genome-wide association studies. However, the worldwide genetic relationships and the migration history of the crop remained uncertain, and genome-wide association studies have produced extensive gene lists related to agronomic traits. Here, we re-analysed the sequenced genomes of 305 cultivated and wild spinach accessions to unveil the phylogeny and history of cultivated spinach and to explore genetic variation in relation to phenotypes. In contrast to previous studies, we employed machine learning methods (based on Extreme Gradient Boosting, XGBoost) to detect variants that are collectively associated with agronomic traits. Variant-based cluster analyses revealed three primary spinach groups in the Middle East, Asiaand Europe/US. Combining admixture analysis and allele-sharing statistics, migration routes of spinach from the Middle East to Europe and Asia are presented. Using XGBoost machine learning models we predict genomic variants influencing bolting time, flowering time, petiole color, and leaf surface texture and propose candidate genes for each trait. This study enhances our understanding of the history and phylogeny of domesticated spinach and provides valuable information on candidate genes for future genetic improvement of the crop.

Prevalence of African Mosaic and Morphological Characterization of Cassava (Manihot esculenta Cranz) Varieties in Two Agroecological Zones of Cameroon

The objective of this work is to conduct an epidemiological study of African cassava mosaic and to characterize the diversity of local cultivars in circulation in the monomodal and bimodal rainforest zones of Cameroon. Disease-related parameters (incidence and severity index) were studied as well as morphological parameters (plant height, leaflet color, petiole color, lobe length, number of lobes, number of branches, petiole length). The results show that 96 cassava varieties were identified with 89 local varieties and 07 improved varieties. Diseased plants showed a symptom severity index of 1 to 5, which could reduce production by half in the areas considered. The leaf color was dark green, the number of branches was 3, the lobe and petiole lengths were 16.46 and 22 cm respectively, while the number of lobes was 5 the disease incidence for the two agro-ecological zones as a whole is 57.58% for a symptom severity index of 3. The epidemiological study reveals that the desease incidence of both agro-ecological zones was 57.58 % for a symptom severity index of 2 corresponding to 40 %. In the humid forest zone with monomodal spray the average incidence was 49.74% and a leaf attack rate was 29.03 % in the locality of Njombé (Cameroon's littoral region). At Ekona in southwestern Cameroon, the average incidence was 63% and leaf attack index was 1 corresponding to 20 %. In the bimodal rainforest zone (central Cameroon region), the average incidence was 60 % for a leaf attack rate was 37.11%. The expected results highlight a relatively worrying situation. Principal Component Analysis (PCA) confirms the convergence of tolerance towards the mosaic and grouped the different varieties into more or less homogeneous groups according to the morphological and epidemiological cassava descriptors. The results of the present work show that the introduction of cassava local varieties tolerant to mosaic disease in middling could be an alternative for the control of the cassava mosaic disease.

Identifikasi Pengaruh Ketinggian Tempat terhadap Morfologi Talas Beneng (Xanthosoma undipes K Koch)

The genetic diversity of beneng taro can be known by analyzing morphological characters. This study aimed to determine the differences in morphological characters in beneng taro plants influenced by altitude using PCA and identify the level of uniformity of morphological characters in beneng taro plants using cluster analysis. This research is a qualitative study with a descriptive approach that is an exploratory survey. Sampling was carried out using a purposive sampling technique. In this study, samples were taken from three different altitudes, namely 343 masl (low), 615 masl (medium), and 761 masl (high) located in Serang Regency and Pandeglang Regency. Ten samples were taken in each location, so 30 taro beneng plants were collected. The research was conducted from January 2024 to March 2024. The variables observed in this study were morphological characters consisting of leaf blade color, leaf bone color, petiole color, plant height, leaf length, leaf width, and petiole length. The results showed that based on PCA, the characters that differed due to altitude were plant height, leaf blade color, leaf bone color, petiole color, petiole length, leaf length, and leaf width. In cluster analysis, 4 clusters were formed, indicating that the uniformity of the characters is relatively broad. Suggestions for further research on root and tuber morphology. Suggestions for farmers to continue cultivating beneng taro. The government should counsel the surrounding community about beneng taro, especially farmers who can preserve local commodities and support conservation efforts.

Morpho-Anatomical Variations of Leaves Several Types of Betel Piperaceae in Durenan District, Trenggalek Regency

The Piperaceae family is a plant species that has 3,700 species. In Durenan District, Trenggalek Regency, there are several species of Piperaceae family plants which have different leaf shapes. This study aimed to describe the morpho-anatomical variations of betel leaves in Durenan District, Trenggalek Regency. This study was a descriptive study to describe three types of betel; green betel, red betel, and china betel. Parameters measured were leaf morphology and leaf anatomy. Variations in betel leaf morphological characters from the Piperaceae family can be seen from leaf shape, leaf color, leaf base, petiole color, leaf length, leaf width, petiole length, and leaf area. Anatomical variations of betel leaf from the Piperaceae family included variations in the number of epidermal layers, the number of hypodermic layers, the number of palisade parenchyma layers, the number of spongy parenchyma layers, stomata shape, stomatal density, and stomata index.

Intraspecific Variability and Phenetic Relationships of <i>Centella Asiatica</i> (L.) Urb. Accessions from Central Java Based on Morphological Characters’

Centella asiatica (L.) Urb. is a plant species native to Java and one of the main basic materials in traditional and modern medicine. This study is the first to report the intraspecific variation and taxonomic relationships of C. asiatica accession from natural populations in Central Java. The purpose of this study was to reveal phenotypic variations of C. asiatica populations and to asses phenetic relationships based on morphological characters. Thirty-two accessions of C. asiatica were collected from natural populations from eight mountains in Central Java. Observation on vegetative organs resulted in 25 morphological characters as a basis for assessing phenetic relationships using cluster analysis and principal component analysis. Result of cluster analysis showed that the grouping of accessions was not correlated to the localities from where the samples were collected, although there was a tendency that accessions from the same localities grouped in one cluster. The results of this study confirmed the existence of intraspecies morphological variability in C. asiatica which was not affected by geographical aspects. Results of principal component analysis indicated that the grouping of accessions was mainly determined by similarities in petiole color, stolon color, leaf margin, petiole length, stolon length, and leaf color. Given that the characters contributing to the grouping of accessions were mainly qualitative characters, the results indicated a genetic basis underlying phenotypic variations of C. asiatica accession.

Characterization and evaluation of the morphological attributes of Ethiopian mustard (Brassica carinata A. Braun) landraces

Ethiopian mustard (Brassica carinata A. Braun) is one of the Brassica leafy vegetables and oilseed crops, which can withstand adverse environmental conditions. This study was conducted to characterize and evaluate the leaf attributes of 313 Ethiopian mustard accessions grown in the greenhouse and field in 2018/19 in Beijing, China. Data on 18 different leaf morphological attributes were collected based on the IBPGR for Brassica and Raphanus descriptor format and Fiji Image J. The collected data were subjected to descriptive statistics using JMP version 14 software. Results indicate that the majority of the accessions had serrated leaf margin, intermediate juvenile development, rounded apex leaf shape, absence of leaf pubescence, number of lobes > 5 and had intermediate number of leaves per plant with frequencies of 101, 213, 223, 244, 183 and 289, respectively. The accessions had also white midrib color, light green leaf color, purple petiole color, purple stem color and purple apex leaf color pigments with frequencies of 168, 165, 104, 113 and 122, respectively. Significant differences were observed among the accessions for leaf size attributes (p < 0.001) indicating their potential as leafy vegetables. Furthermore, there were significant accession × environment effects for the laminal length, total leaf length, leaf area, leaf weight and petiole length. Notably, 71% of the accessions had an average of 81 days flowering time. The multivariate cluster and principal component analyses divided the 313 accessions into eight groups with distinctive information; consisting of 81, 37, 79, 38, 14, 11, 6 and 47 accessions. Thus, this study provides important information about Ethiopian mustard germplasms that is vital for further advancement and genetic improvement of the crop.

Ethnobotany of cassava (Manihot esculenta Crantz) in the traditional peri-urban Sundanese community of Cireundeu Hamlet, Cimahi City, West Java, Indonesia

Abstract. Irawan B, Safira KM, Annisa, Rohmatullayaly EN, Ihsan M, Suryana, Iskandar J, Iskandar BS. 2024. Ethnobotany of cassava (Manihot esculenta) in the traditional peri-urban Sundanese community of Cireundeu Hamlet, Cimahi City, West Java, Indonesia. Biodiversitas 25: 116-128. The community of Cireundeu Hamlet is one of the Sundanese traditional communities that still maintain wisdom in processing and using cassava (Manihot esculenta Crantz) as staple food (rasi) and foodstuff. Many landraces of cassava have the potential to develop into superior varieties, and research is necessary to make cassava a year-round food. The aim of this study is to elucidate the ethnobotanical knowledge of the rural Sundanese community on cassava, namely on various landraces and their folk classification, traditional farming management, and uses. Based on a case study of the traditional people in a peri-urban area of Cireundeu Hamlet, Cimahi City, West Java, Indonesia, mixed methods, i.e., qualitative and quantitative, were used in this study. There are seven landraces of cassava used as foodstuff that are traditionally managed by the Cireundeu people, including sampeu apuy, sampeu bodas, sampeu gambir lembang, sampeu garnawis, sampeu hideung, sampeu karihkil, and sampeu mentega. Based on Relative Frequency of Citation, the highest values are sampeu bodas (0.96) and sampeu garnawis (0.93). These cassava landraces can be distinguished by the Cireundeu people based on characteristics of petiole color, stem color, apical leaf color, leaf color, and taste of the tubers. Based on folk taxonomy, cassava is classified into five levels: level 0 (unique beginner), known as tutuwuhan and pepelakan (plants); level 1 (Life form) known as beubeutian (tubers); level 2 (generic) known as sampeu karet and sampeu, a genus from Manihot esculenta; level 3 (species) known as sampeu (sweet-cassava, Manihot esculenta Crantz) and sampeu karet (bitter cassava, M. carthagenensis subsp. glaziovii); and level 4 (specific). The processing and use of cassava are carried out both in traditional and modern ways, including using apical leaves as lalab or boiled vegetables and using the roots as the basic ingredients for making rasi (sangueun), tapioca flour, and snacks.

Morphological Characterization of Purple Yam (Dioscorea alata) in Purwodadi Botanic Garden

Dioscorea spp. (Yam) is an edible tuber plant from the Dioscoreaceae family. There are around 600 species of Dioscorea spp., one of them is Dioscorea alata. D. alata has a wide range of morphological characteristics, including shape, size, skin color, flesh, and tuber texture. This research focused on D. alata with purple tuber flesh. This study aims to reveal the diversity of D. alata in Purwodadi Botanical Garden based on its morphological characteristics. Characterization is an important aspect of identifying D. alata diversity. Characterization was performed on six D. alata accessions from the Purwodadi Botanical Garden, DA 28, DA 36, DA 57, DA 86, DA-SB, and DA-Tr. Morphological characterization can be divided into qualitative and quantitative stem and leaf properties. The morphological feature data were processed using NTSys 2.11a software. The observation results showed that D. alata from different accessions had diverse morphological characteristics such as stem color, wing color, leaf margin color, leaf vein color, petiole color, and petiole wings color. The dendrogram results showed that DA 36 and DA SB accessions had a very close level of similarity. Meanwhile, the DA-Tr accession exhibited the most distinct character from the other accessions with the dominant color being purple covering stems, stem wings, leaf veins, leaf margins, petioles, and petiole wings.

QTL Mapping and Genetic Map for the Ornamental Sunflower in China

Quantitative trait locus (QTL) mapping and genetic map are of great significance for ornamental sunflowers in China. In this study, a total of 956.50 Mbp data were obtained, the average Q30 was 93.76%, the average GC content was 42.43%, and the GC distribution of the parents and F2 population of the ornamental sunflowers was normal. At the same time, the double-end comparison efficiency of control data was 90.28%, and the enzyme digestion efficiency was 92.01%. The Specific-Locus Amplified Fragment (SLAF) library construction was normal. Furthermore, a total of 734,893 SLAF markers were obtained, among which 127,855 were polymorphic SLAF markers and 38,908 could be used for genetic map construction, and the effective polymorphism of the parents was 5.29%. Moreover, we constructed a total of 17 linkage groups, with 6181 markers in the QTL mapping, the total map distance was 2608.66 cM, the marker integrity in the figure above was 99%, the proportion of double exchange was 0.05, the sequencing depth of the parents was 42.455 × , and the progeny was 9.24 × . The relationship of traits (plant height, stem diameter, disk diameter, number of petals, leaf number, stigma color, petal color, petiole color) and QTL mapping was closely related to show the best of ornamental effect.

Morphometric diversity of some Nigerian accessions of Bambara groundnut (Vigna subterranea)

AbstractThis study evaluated the morphological diversity of some Nigerian accessions of Bambara groundnut. Field experiments were conducted in Nigeria in two locations and the phenotypic variation of Bambara groundnut accessions evaluated in 3 years (2017–2020) using a randomized complete block design. Twenty-eight quantitative and 10 qualitative traits were observed. Twenty-three out of the 28 quantitative morphological traits showed significant differences. TVSu-589 (28.85) and TVSu-670 (28.57) had the highest plant height; TVSu-572 (121.52), TVSu-271 (113.10) and TVSu-336 (104.15) had the highest 100-seed weight. Genetic variations were also observed in seed colour, terminal leaflet shape, petiole colour and eye pattern. Principal component analysis showed PC1 constituting 23.36% and PC2 constituting 15.76% of the total variation, while the first eight principal components with eigenvalues ≥1 revealed 77.28% of the total variation. Cluster analysis grouped the accessions into four groups. Based on yield data in this study, accessions TVSu-594, TVSu-350, TVSu-336, TVSu-1242, TVSu-129, TVSu-14, TVSu-179, TVSu-2100, TVSu-261 and TVSu-589 were the best for yield and are recommended for further evaluation to improve yield. This study showed that a wide range of diversity exists in Bambara groundnut of Nigerian origin that could be useful for further utilization of genetic resources and improvement.

Morphological Characteristics and Similarity Analysis of Cassava (Manihot esculenta Crantz) in Wonosobo, Temanggung, and Magelang Regencies

The diversity of cassava in Wonosobo, Temanggung and Magelang districts must be investigated. The objective of the study was to analyze the diversity of cassava based on morphological characteristics. The cassava of each accession was planted in Bandongan, Magelang Regency. Morphological characters observed after six months of planting included apical leaf color, apical leaf hair, leaf lobe shape, petiole color, leaf color, number of leaf lobes, leaf length, leaf width, petiole length, vein color, petiole orientation, color epidermis of stem, color of outer appearance of stem, distance between nodes, stem growth, color of mature branch tip, branching type, tuber, tuber shape, outer tuber skin color, tuber flesh color, and tuber inner skin color. Analysis of the morphological characteristics of cassava is presented in the form of a dendrogram using the MVSP (Multi Variate Statistical Package) version 3.22. The results of the study obtained 39 accessions of cassava which showed different morphological characteristics. The lowest morphological similarity (0.735) was found in Group I only consisted of 2 acquisitions that did not have tubers, namely Red Vegetables and Green Vegetables and Group II ( Groups III and IV) have a similarity of 0.92. The highest similarity was in groups IIIa and IIIb and Groups IVa and IVb have a similarity coefficient value of 0.95. The morphological diversity of 39 accessions is a source of cassava germplasm: 37 accessions are harvested for tubers and 2 accessions areharvested explicitly for leaves as vegetables. This research was useful for the development of cassava-based food industry bioproducts.

Phenotypic Characterization, Evaluation, and Classification of Cassava (Manihot esculenta Crantz) Accessions in Ethiopia

Cassava has a crucial role in benefiting smallholder farmers as the main food and income source in southern Ethiopia. Characterization accessions are crucial for assessing variation, classifying, and identifying desirable accessions for crop improvement and conservation. In this regard, there needs to be more information on the morphological characterization and classification cassava accessions. Thus, the aim of this research was to systematically characterize, evaluate, and classify cassava accessions using qualitative characters to provide useful information for breeding program and conservation. A total of 64 accessions were planted using a simple lattice design during the 2020-2021 cropping season. Thirty qualitative data were collected at 3, 6, 9, and 18 months after planting and analyzed using the SAS and R-software packages. The high variable characteristics were the shape of the central leaflet, petiole color, leaf retention, branching habit, the color of the stem epidermis, the color of the stem exterior, the external color of the storage root, and the color of the root pulp towards frequency distribution analysis. The Shannon–Weaver diversity index ranged from 0.24 to 1.47, with an overall mean of 0.84. The first three dimensions in the multiple correspondent method explained approximately 39.39% of the total variation, with Dim 1 accounting for 20.77% and Dim 2 accounting for 9.98%, while petiole color and texture of the root epidermis were the leading contributors to the total variation, respectively. In clustering analysis, 64 accessions were classified into 4 clusters of varying sizes. The distribution of accessions in each cluster revealed that 52 accessions were in cluster I, 6 in cluster II, 5 in cluster III, and 1 in cluster IV. Each cluster was varied by a major group characteristic that it represented. Furthermore, the study identified the desirable accessions for desired storage root characteristics such as root constrictions, the external color of the storage root, the color of root pulp, the color of the root cortex, and cortex peeling tendency. In conclusion, the various analyses performed indicated the existence of sufficient genetic variability for the characteristics evaluated, which could be attributed to the dissimilar genetic backgrounds of the evaluated accessions. Thus, these could be utilized for breeding work and conservation.

IDENTIFICATION OF MORPHOLOGY AND RELATIONSHIPS OF PORANG PLANTS (AMORPHOPHALLUS MUELLERI BLUME) IN KUMPEH ULU DISTRICT, MUARO JAMBI REGENCY

Porang (Amorphophallus muelleri Blume) is a group of tuber plants that are useful as food and medicine so that they have bright prospects for development. In Jambi Province, especially in Muaro Jambi Regency, porang plants are found growing wild among duku trees. This study aims to determine the morphological character of porang plants and to determine the level of diversity and kinship of porang plants found in Kumpeh Ulu District, Muaro Jambi Regency. The research was carried out from August to September 2021, in 18 villages located in the Kumpeh Ulu District. The research was conducted using a non-experimental descriptive method with proportionate stratified random sampling, and the sample was measured quantitatively and qualitatively. The morphological identification of porang plants from 18 villages in Kumpeh Ulu District showed similarities in bulbil shape, bulbil surface colour, bulbil surface texture, bulbil flesh colour, leaf shape and petiole colour. However, there were differences in leaf colour, tuber flesh colour, tuber surface texture and tuber shape.

IDENTIFICATION AND CHARACTERIZATION OF CASSAVA MUTANT GENOTYPES WITH HIGH LEAF MINERAL CONTENT AT THE MV10 GENERATION

Cassava leaves’ consumption as vegetables are common in several Asian countries. They contain various nutrients, such as, anthocyanins, carotene, minerals, and vitamins. New varieties with high mineral contents in leaves need development to increase the leaves’ quality as vegetables. This study aimed to identify and characterize cassava genotypes of the MV10 generation resulting from gammaray- induced mutations based on the leaves' morphology, growth characteristics, and mineral contents, using two cultivars and 10 cassava genotypes. All genotypes planted in a randomized complete block design had three replications. The leaf's morphology and mineral contents underwent scrutiny on the third to fifth leaf below the apical shoot. The mineral content analysis used the Atomic Absorption Spectrophotometry method. Data analysis comprised the ANOVA test and descriptive analysis. This study revealed petiole length, leaf lobe length, and leaf lobe width were significantly different between genotypes at four months after planting (MAP). However, no meaningful difference showed for the plant height, stem diameter, and number of leaves among genotypes. In general, the leaf morphology consisted of purplish green apical leaves with present pubescence; the shape of the central leaflet is lanceolate; the petiole color is reddish to greenish; leaves are dark green with five to seven leaf lobes; leaf veins are reddish-green; smooth lobe margins; and horizontal petiole orientation. The genotypes affect mineral contents, as the genotype with the highest Mg contents was G6-2-15-5-3, and the one with the highest Zn contents was G2D1-422. Fe contents showed more variations between genotypes, and no genotype showed consistently high Fe contents. This research produced promising genotypes for Mg or Zn contents in leaves for future cassava varieties for vegetable production.

Gene exchange and dissection of genetic character associations among Okra

Gene exchange and hybridization of okra [Abelmoschus esculentus (L.) Moench] are important for the development and utilization of new varieties. To study the existing diversity in attributes of okra germplasm and their potential for successful hybridization. The modes of inheritance of leaf shape, stem color, stem pubescence, leaf pubescence, petiole color, leaf color, fruit color, and fruit pubescence were studied to evaluate the possibility of gene exchange among okra varieties. Five okra varieties were characterized using morphological descriptors and parent plants were hybridized in all combinations. The parents, the F1 and F2 plants grown in the field were evaluated for growth and yield-related factors. The qualitative descriptors leaf shape and pubescence, presence, or absence of, anthocyanin in the leaf and stem, and chlorophyll intensity in leaves which are distinct contrasting characters were used for the mode of inheritance. Data collected based on the descriptors were analyzed for variance among parents and hybrid progeny. A chi-squared test was performed to determine the goodness of fit to the expected ratios in the F2 generation. There is significant variation (p<0.05) between parents and their crosses for vegetative growth and yield. All hybrids are elite as they started fruiting early. Most of the crosses performed better than their parents for growth and fruit-related characters. All qualitative characters in the F2 generation were monogenically controlled. Patterns of segregation in the F2 generation was statistically consistent with ratios of 3:1, 1:1 and 1:2:1, indicating that they are controlled by a single gene with complete (3:1 phenotypic ratio) and incomplete (1:2:1 phenotypic ratio) dominance. The gene exchange and mode of inheritance of characters among segregating generations provide information on which selection can be made to improve okra.

Multivariate analysis of seed yield and morphological characters of Okra (Abelmoschus esculentus L. Moench) accessions

Abstract. Enhancement of okra fruit production requires identification of genotypes with promising seed yield attributes, thus multivariate techniques were employed to reveal genetic and morphological attributes of sixteen diverse okra lines during wet and dry seasons in 2019 at Teaching and Research Farm, College of Agriculture, Osun State University, Ejigbo Campus. Seeds were sown in single-row plots of 5 m long, spaced 0.70 m apart and 0.50 m within on sandy loam soil in a randomized complete block design. The dry season crop was supported by the application of 12 mm water weekly. Data collected on growth and seed traits were subjected to the General Linear Model (GLM), principal component analysis (PCA), and cluster analysis using the Statistical Analysis System (SAS, 2018). The petiole color was scored according to IPGRI (1991) square root transformed prior to the analysis. Results showed highly significant lines, season, and their interactions for almost all traits. Four PCAs accounted for 85.77% and the first two PCA captured 51% of the total variations. Both PCA and cluster analysis grouped the lines into four and revealed the potentials of SAHARI F1, NGB01197, and LD-88 for high seed yield. It is concluded that these lines can be incorporated into okra yield improvement program.

Mapping of the AgPPur gene for the purple petiole in celery (Apium graveolens L.)

Celery (Apium graveolens L.) is a vegetable widely planted and eaten all over the world, and its petiole is the main edible part. To date, there are very few reports about the inheritance and gene cloning of celery petiole color. In this study, based on the purple petiole celery inbred line (ppur) discovered in the early stage of our research, its petiole is purple, and its dwarf stem and leaves are green. The F2 segregation population was configured by crossing it with inbred lines with white petioles. The genetic analysis showed that the purple petiole to white petiole segregation ratio was 3:1 and that the purple trait was dominantly inherited under single gene control. The ppur locus was localized within 324 kb on Chr09 by map cloning, and the gene AgPPur, whose homolog in Arabidopsis encodes the NAC transcription factor, was targeted by RT‒PCR and sequence comparison. Sequencing of both parents revealed a 4-bp deletion at the 5′ UTR in white petiole material, and the gene marker Indel-A4 developed based on this deletion was 100% accurate in both F2 populations. Validation in 30 celery materials with different colored petioles in the laboratory revealed that the marker could perfectly distinguish between purple/nonpurple petiolar materials. Therefore, AgPPur was considered to be the candidate gene controlling purple petioles in celery. Our results could not only improve the efficiency and accuracy of celery breeding but also help to understand the mechanism of anthocyanin synthesis in celery.

Combined evaluation of agronomic and quality traits to explore heat germplasm in celery (Apium graveolens L.)

Existing celery germplasm resources must be evaluated prior to celery biological research and application of germplasm resources. In this study, 14 agronomic and quality traits, and heat-resistance of 50 celery cultivars were analyzed. The results showed that the coefficients of variation of the 14 traits ranged from 1.73% to 64.69%, 50 samples of celery could be divided into 5 groups by cluster analysis. The primary sources of variation in agronomic traits were petiole color, hollow/solid, and yield per plant, and the primary sources of variation of quality traits were apigenin and cellulose. The photosynthetic parameters and heat damage index was investigated, and the correlation between celery germplasm resources and heat-resistance was analyzed. The result showed that heat-resistance was correlated with its hollow/solid and color. This study analyzed the genetic diversity of celery germplasm resources, screened heat-resistant and heat-sensitive varieties, and performed an association analysis with the germplasm resources, which laid the foundation for the subsequent selection of heat-resistant celery varieties.

IDENTIFIKASI JENIS-JENIS UBI KAYU (Manihot esculenta) DI KECAMATAN TOMIA TIMUR KABUPATEN WAKATOBI

This study aims to identify and describe the types of cassava in Tomia Timur District, Wakatobi Regency. The research was carried out in Tomia Timur Subdistrict, Wakatobi Regency, in December 2021 January 2022. This research is a survey research that is analyzed descriptively by statistics that is describing, describing, and describing the object being studied. The variables observed were stem color, branched and unbranched, stem height, stem diameter, distance between stem segments, leaf color, petiole color, leaf lobe length, tuber shape, wet tuber weight per tree, outer skin color of tuber, color inner skin of tubers, tuber flesh color, benefits of cassava plants, cultivation techniques, and soil conditions. The results showed that there were 10 types of cassava in Tomia Timur District, Wakatobi Regency, namely Kalambe cassava, Kalambe Fanse cassava, Ndoke cassava, Bogoro cassava, Bogoro Mohoro cassava, Makuri cassava, Andapatua cassava, Salbia cassava, cassava English wood, and Andabula cassava.

Diversity, classification, and EST‐SSR‐based association analysis of caladium ornamental traits

Caladium (Caladium × Hortulanum Birdsey) is a popular ornamental plant with a wide range of vibrant leaf color among Araceae. Even after years of breeding, creating new caladium leaf color variations is extremely difficult. Molecular marker-assisted selection is an effective approach for accelerating breeding, but few studies on the molecular markers associated with caladium traits have been performed. In the current study, 144 caladium accessions were used to examine 12 phenotypic characteristics. The coefficient of variation for four numerical characters ranged from 23.94% to 43.22%, and the Shannon-Wiener indexes for eight descriptive characters ranged from 0.13 to 1.52. Based on L*, a*, b*, C, h° values determined by a colorimeter and hierarchical cluster analysis, the leaf color can be divided into four groups: pale green, green, light pink, and red. Furthermore, 7708 new SSR loci were identified by transcriptome sequencing, and 26 SSR markers with high polymorphism and reproducibility were screened. Genetic structure, NJ clustering, and PCoA analysis revealed that 144 accessions could be divided into three clusters, with genetic structure being closely related to germplasm origin. An association analysis revealed that the SSR markers 2, 1, 1, 1, 1, and 1 were mainly associated with petiole color, main vein color, blade upperside glossiness, and C, b*, and L* of leaf color (p < 0.01). These findings will serve as a valuable reference for evaluating germplasm resources and caladium molecular marker-assisted breeding.