Pesticide residues in fruits and vegetables present a significant concern for human health and safety. By 2022, an average of 3 million people worldwide is poisoned by pesticides every year, and the mortality rate can reach about 20%. This comprehensive review summarizes recent research on the detection of pesticide residues, focusing on the main detection methods and their implications. The study highlights the growing importance of biosensors as a prominent technique, offering enhanced efficiency and accuracy in pesticide residue analysis. The review addresses the challenges associated with pretreatment methods and discusses the advantages and limitations of biosensors. Furthermore, it emphasizes the need for further research to optimize the adaptive capabilities of biosensors, particularly their anti-interference abilities. The findings underscore the significance of developing intelligent adaptive sensors for on-site pesticide residue detection, eliminating the need for complex sample pretreatment. This comprehensive review serves as a valuable reference, facilitating future advancements in pesticide residue analysis, ensuring food safety, and safeguarding consumer health in modern agriculture.