Doses Of Pesticides Research Articles

A Study on the Diversity of Pesticide-Resistant Bacterial Population from Different Agricultural Fields of Manjoor

The regular usage of pesticides in agricultural fields results in the development of a pesticide-resistant microbial population. Vegetable cultivation is a common practice in the agricultural growing areas of Manjoor, Kerala. The present study was envisaged to understand the resistance of microorganisms to different types and doses of pesticides. The study revealed that heterotrophic bacteria are capable of resisting lower concentrations (0.01 and 0.001%) of the pesticides lindane and methyl parathion while a higher concentration of carbaryl (0.1%) could also be tolerated. In the soil sample where there was no prior addition of pesticides, the heterotrophic bacteria could only tolerate very low concentrations of pesticides The results of mean pesticide-resistant bacterial load when compared to normal Total Heterotrophic Bacteria (THB) of soils indicate that pesticides exhibited an inhibitory effect on the heterotrophic bacteria of soils collected from different agricultural fields and the pesticide-resistant bacterial load was lower than normal THB.

A comparative study on the adsorption behavior and mechanism of pesticides on agricultural film microplastics and straw degradation products.

Straw will degrade into segment, powder and crystalline cellulose, while the agricultural film will degrade into microplastics (MPs) in farmland soils. The specific surface area of these micro-particles increases and many new functional groups are formed in the degradation process, which can be a good vector of pesticides. To more accurately and truly analyze the risk of main imported substances and their degradation products against pollutants in soil, the adsorption behavior and mechanism of four commonly used pesticides on aged polyethylene microplastics (APE), wheat straw segment (WSS), wheat straw powder (WSP), and straw crystalline cellulose (SCC) were analyzed and compared through batch adsorption experiments and infrared spectrum. The adsorption kinetics of four pesticides on MPs and straw degradation products tended to be pseudo-second-order kinetics; the adsorption isotherms of pesticides on APE and SCC tended to fit the Freundlich model, while on WSP and WSS tended to fit the Langmuir model. The adsorption was a spontaneous endothermic increase process, suggesting that the main adsorption force of pesticides on MPs and straw degradation products was hydrophobic diffusion. The adsorption of pesticides against WSP and WSS still had a certain π-π conjugation and electrostatic interaction. And the adsorption amount on the straw degradation products followed the order of WSP>WSS>APE>SCC, presumably related to the specific surface area and pore volume of the adsorbent. As WSP, WSS could adsorb more pesticides, the straw returning to the field can be used for slow-release of pesticides to reduce the dosage of pesticides.

Polysaccharides synergistic boosting drug loading for reduction pesticide dosage and improve its efficiency

Overuse of pesticides is an urgent issue to be solved in sustainable agriculture. Based on the synergistic drug loading effect of β-cyclodextrin (β-CD) encapsulation and alginate (Alg) cross-linking, a new environment-responsive drug delivery system (TMX-loaded Alg/β-CD) was constructed. The relationship between carrier structure and solubility of thiamethoxam (TMX) was researched by molecular simulation. β-CD has good binding affinity with TMX, which can increase TMX solubility by 40 %. Further co-loading with alginate could double the drug loading of the cyclodextrin inclusion complex up to 41 %. TMX-loaded Alg/β-CD exhibits excellent environment-responsive controlled-release performance, and TMX sustained release time is 7.5 times longer than that of commercial agents. The pest control efficacy of TMX-loaded Alg/β-CD is 20 days longer than that of commercial TMX, and the crops has no pesticide residues after using. This study provides a promising strategy for the commercial application of polysaccharide in pest control and pesticide reduction.

Feeding on grains containing pesticide residues is detrimental to offspring development through parental effects in grey partridge

Numerous toxicological studies have shown that ingestion of pesticides can induce physiological stress in breeding birds, with adverse consequences on egg laying parameters and offspring quality through parental effects. However, previous studies do not mimic current levels of pesticide residues in typical landscapes, and they do not consider potential cocktail effects of pesticides as they occur in the wild. Herein, we explored whether realistic pesticide exposure affected reproduction parameters and offspring condition through parental effects in Grey partridge. We fed 24 breeding pairs with either seeds from conventional agriculture crops treated with various pesticides during cropping, or organic grains without pesticide residues as controls. The conventional and organic grain diets mimicked food options potentially encountered by wild birds in the field. The results showed that ingesting low pesticide doses over a long period had consequences on reproduction and offspring quality without altering mortality in parents or chicks. Compared with organic pairs, conventional pairs yielded smaller chicks at hatching that had a lower body mass index at 24 days old. Additionally, these chicks displayed lower haematocrit when body mass index was higher. Therefore, ingestion of conventional grains by parents resulted in chronic exposure to pesticide residues, even at low doses, and this had detrimental consequences on offspring. These results demonstrate a sublethal effect of pesticide residues through parental effects. The consequences of parental exposure on chicks might partly explain the decline in wild Grey partridge populations, which raises questions for avian conservation and demography if current agrosystem approaches are continued.

Graphene-Delivered Insecticides against Cotton Bollworm.

Nanopesticides can facilitate controlled release kinetics and efficiently enhance the permeability of active ingredients to reduce the dosage and loss of pesticides. To clarify the synergistic mechanism of graphene–insecticide nanocarriers against cotton bollworm, treatment groups, namely, control, graphene (G), insecticide (lambda-cyhalothrin (Cyh) and cyfluthrin (Cyf)), and graphene-delivered insecticide groups were used to treat the third-instar larvae of cotton bollworm. The variations in phenotypes, namely, the body length, body weight, and mortality of the cotton bollworm, were analyzed. The results show that graphene enhances the insecticidal activity of lambda-cyhalothrin and cyfluthrin against cotton bollworm. The two graphene-delivered insecticides with optimal compositions (3:1) had the strongest inhibitory effects and the highest mortality rates, with the fatality rates for the 3/1 Cyh/G and Cyf/G mixture compositions being 62.91% and 38.89%, respectively. In addition, the 100 μg/mL Cyh/G mixture had the greatest inhibitory effect on cotton bollworm, and it decreased the body length by 1.40 mm, decreased the weight by 1.88 mg, and had a mortality rate of up to 61.85%. The 100 and 150 μg/mL Cyh/G mixtures achieved the same mortality rate as that of lambda-cyhalothrin, thus reducing the use of the insecticide by one-quarter. The graphene-delivered insecticides could effectively destroy the epicuticle spine cells of the cotton bollworm by increasing the permeability and, thus, the toxicity of the insecticides.

An Investigation of the Neurotoxic Effects of Malathion, Chlorpyrifos, and Paraquat to Different Brain Regions

Acute or chronic exposures to pesticides have been linked to neurotoxicity and the potential development of neurodegenerative diseases (NDDs). This study aimed to consider the neurotoxicity of three widely utilized pesticides: malathion, chlorpyrifos, and paraquat within the hippocampus (HC), corpus striatum (CS), cerebellum (CER), and cerebral cortex (CC). Neurotoxicity was evaluated at relatively low, medium, and high pesticide dosages. All pesticides inhibited acetylcholinesterase (AChE) and neuropathy target esterase (NTE) in each of the brain regions, but esterase inhibition was greatest in the HC and CS. Each of the pesticides also induced greater disruption to cellular bioenergetics within the HC and CS, and this was monitored via inhibition of mitochondrial complex enzymes I and II, reduced ATP levels, and increased lactate production. Similarly, the HC and CS were more vulnerable to redox stress, with greater inhibition of the antioxidant enzymes catalase and superoxide dismutase and increased lipid peroxidation. All pesticides induced the production of nuclear Nrf2 in a dose-dependent manner. Collectively, these results show that pesticides disrupt cellular bioenergetics and that the HC and CS are more susceptible to pesticide effects than the CER and CC.

Pesticide risk assessment: honeybee workers are not all equal regarding the risk posed by exposure to pesticides.

Toxicological studies in honeybees have long shown that a single pesticide dose or concentration does not necessarily induce a single response. Inter-individual differences in pesticide sensitivity and/or the level of exposure (e.g., ingestion of pesticide-contaminated matrices) may explain this variability in risk posed by a pesticide. Therefore, to better inform pesticide risk assessment for honeybees, we studied the risk posed by pesticides to two behavioral castes, nurse, and forager bees, which are largely represented within colonies and which exhibit large differences in their physiological backgrounds. For that purpose, we determined the sensitivity of nurses and foragers to azoxystrobin (fungicide) and sulfoxaflor (insecticide) upon acute or chronic exposure. Azoxystrobin was found to be weakly toxic to both types of bees. However, foragers were more sensitive to sulfoxaflor than nurses upon acute and chronic exposure. This phenomenon was not explained by better sulfoxaflor metabolization in nurses, but rather by differences in body weight (nurses being 1.6 times heavier than foragers). Foragers consistently consumed more sugar syrup than nurses, and this increased consumption was even more pronounced with pesticide-contaminated syrup (at specific concentrations). Altogether, the stronger susceptibility and exposure of foragers to sulfoxaflor contributed to increases of 2 and tenfold for the acute and chronic risk quotients, respectively, compared to nurses. In conclusion, to increase the safety margin and avoid an under-estimation of the risk posed by insecticides to honeybees, we recommend systematically including forager bees in regulatory tests.

Ameliorative effect of L-carnitine on lambda-cyhalothrin-induced anatomical and reproductive aberrations in albino mice

The objective of this research was to look at how the pesticide lambda-cyhalothrin (LCT) affected the liver, kidney, testis, and ovary of albino mice; and on morphological and skeletal features of the newborn of treated females. The study also aimed to test the ameliorative effects of L-carnitine (LC) against (LCT). Five sets of mice were created, Group 1 acted as the control, while Group 2 received a high dose of LCT, Group 3 received a high dose of LCT + LC, Group 4 received a low dose of LCT that was a residue of in khat (Qat), and Group 5 received a low dose of LCT + LC. The findings revealed that the treated groups' body weights were reduced significantly, whereas the absolute and relative weights of the liver in all groups were statistically decreased insignificantly. There were histopathological changes in the tissues in groups 2 and 4. While the tissues of the ovary and testis showed recovery in groups 3 and 5. When compared to the control group, the values of the seminiferous tubules parameters were statistically significant in the 3 and 5 groups. The newborn had a high dose of pesticides and showed some malformations in the skeleton. However, in group 3 the skeletal malformation was minimized and in-group 5 the skeleton malformations had completely disappeared. It could be concluded that LCT is highly harmful to mouse tissues and caused neonatal malformations, whereas LC has a marked protective effect against LCT.

Fungicide and Arbuscular Mycorrhiza Fungi Applications in Tomato

Tomato is one of the important food crops of the world. It has rich essential nutrients features. However, tomato plants are sensitive to certain diseases and pests. This situation causes intense and unconscious pesticide use to avoid crop losses. It is known that mycorrhiza provide many advantages to plant. In this study, the effects of different doses of fungicide applications on some physiological parameters were examined in mycorrhiza applied and non-applied mycorrhiza tomato plants. A pesticide was applied at different doses which were, namely, recommend (R), half of recommend (R/2), and two-fold recommend (R×2). The content of proline, chlorophyll and carotenoid analysis were conducted in the plant samples. Proline values were found low in mycorrhizal than non-mycorrhizal plants in all pesticide doses (P

INTERACTION AMONGST THE CHEMICAL PESTICIDES, AGRICULTURE AND HUMAN HEALTH: AN ENVIRONMENTAL CASE STUDY IN NADIA DISTRICT, WEST BENGAL, INDIA

Chemical pesticides and fertilizers are well known in today’s farming system and played asignificant role in boosting the crop yield during the last four to five decades worldwide. But on the otherhand, excessive use of these chemicals has been degrading natural resources (soil, water, air) globally andleads to increase environmental pollution. With this background, the broader objective has been set as‘assessing the impact of chemical pesticides and fertilizers on the soil, water, air vis-à -vis human beings andlocal biodiversity, a study was carried out in two different villages (Ghoragachha and Bhabanipur) of Nadiadistrict, West Bengal, India with 200 number of respondents. The majority of the farmers belongs tomarginal and small classes and is mainly vegetable growers. They also cultivate fruits and a few cultivateagronomic crops with a cropping intensity of greater than 200 percent. Most of the respondents don’t takeprecautionary measures during pesticide storage, handling, and storing due to illiteracy among therespondents. Excess use of pesticides just increases the face value of the produce. They have no suchawareness about crop-pest resistance and soil contamination. Indiscriminate use and application ofimproper doses of pesticides may cause their symptoms as well as the environment.

Fabrication and Characterization of Antifungal Hydroxypropyl-β-Cyclodextrin/Pyrimethanil Inclusion Compound Nanofibers Based on Electrospinning.

Pyrimethanil (PMT) is an anilinopyrimidine bactericide with poor water solubility, which limits its applications. To improve the physical and chemical properties of PMT, hydroxypropyl-β-cyclodextrin/pyrimethanil inclusion compound nanofibers (HPβCD/PMT-IC-NFs) were fabricated via electrospinning. A variety of analytical techniques were used to confirm the formation of the inclusion compound. Scanning electron microscopy image displayed that HPβCD/PMT-IC-NF was homogeneous without particles. Thermogravimetric analysis indicated that the formation of the inclusion compound improved the thermostability of PMT. In addition, the phase solubility test illustrated that the inclusion compound formed by PMT and HPβCD had a stronger water solubility. The antifungal effect test exhibited that HPβCD/PMT-IC-NF had better antifungal properties. The release experiment confirmed that HPβCD/PMT-IC-NF had a sustained-release effect, and the release curve conformed to the first-order kinetic model equation. In short, the fabrication HPβCD/PMT-IC-NF inhibited improved solubility and thermostability of PMT, thus promoting the development of pesticide dosage form to water-based and low-pollution direction.

Effects of agricultural pesticides on the susceptibility and fitness of malaria vectors in rural south-eastern Tanzania

BackgroundAgricultural pesticides may exert strong selection pressures on malaria vectors during the aquatic life stages and may contribute to resistance in adult mosquitoes. This could reduce the performance of key vector control interventions such as indoor-residual spraying and insecticide-treated nets. The aim of this study was to investigate effects of agrochemicals on susceptibility and fitness of the malaria vectors across farming areas in Tanzania.MethodsAn exploratory mixed-methods study was conducted to assess pesticide use in four villages (V1–V4) in south-eastern Tanzania. Anopheles gambiae (s.l.) larvae were collected from agricultural fields in the same villages and their emergent adults examined for insecticide susceptibility, egg-laying and wing lengths (as proxy for body size). These tests were repeated using two groups of laboratory-reared An. arabiensis, one of which was pre-exposed for 48 h to sub-lethal aquatic doses of agricultural pesticides found in the villages.ResultsFarmers lacked awareness about the linkages between the public health and agriculture sectors but were interested in being more informed. Agrochemical usage was reported as extensive in V1, V2 and V3 but minimal in V4. Similarly, mosquitoes from V1 to V3 but not V4 were resistant to pyrethroids and either pirimiphos-methyl or bendiocarb, or both. Adding the synergist piperonyl butoxide restored potency of the pyrethroids. Pre-exposure of laboratory-reared mosquitoes to pesticides during aquatic stages did not affect insecticide susceptibility in emergent adults of the same filial generation. There was also no effect on fecundity, except after pre-exposure to organophosphates, which were associated with fewer eggs and smaller mosquitoes. Wild mosquitoes were smaller than laboratory-reared ones, but fecundity was similar.ConclusionsSafeguarding the potential of insecticide-based interventions requires improved understanding of how agricultural pesticides influence important life cycle processes and transmission potential of mosquito vectors. In this study, susceptibility of mosquitoes to public health insecticides was lower in villages reporting frequent use of pesticides compared to villages with little or no pesticide use. Variations in the fitness parameters, fecundity and wing length marginally reflected the differences in exposure to agrochemicals and should be investigated further. Pesticide use may exert additional life cycle constraints on mosquito vectors, but this likely occurs after multi-generational exposures.Graphical

Sodium Chloride (NaCl)-Induced Physiological Alteration and Oxidative Stress Generation in Pisum sativum (L.): A Toxicity Assessment.

Salinity stress has a deleterious impact on plant development, morphology, physiology, and biochemical characteristics. Considering the NaCl-induced phytotoxicity, current investigation was done to better understand the salt-tolerant mechanisms using Pisum sativum L. (pea) as a model crop. Generally, NaCl resulted in a progressive decrease in germinative attributes and physiological and biochemical parameters of P. sativum (L.). The 400 mM NaCl level had a higher detrimental effect and reduced the germination rate, plumule, radicle length, and seedling vigor index (SVI) by 78, 89, 84, and 77%, respectively, under in vitro. Furthermore, after 400 mM NaCl exposure, physiological and enzymatic profiles like root dry biomass (71%) chl-a (66%), chl-b (54%), total chlorophyll (45%), and nitrate reductase activity (NRA) (59%) of peas were decreased. In addition, a NaCl dose-related increase in soluble protein (SP) and sugar (SS), Na+ and K+ ions, and stressor metabolites was recorded. For instance, at 400 mM NaCl, SP, SS, Na+ ion, K+ ion, root proline, and malondialdehyde (MDA) contents were significantly and maximally elevated by 65, 33, 84, 79, 85, and 89%, respectively, compared to the control (0 mM NaCl). Data analysis indicated that greater doses of pesticides dramatically increased reactive oxygen species (ROS) levels and induced membrane damage through production of thiobarbituric acid reactive substances (TBARS), as well as increased cell injury. To deal with NaCl-induced oxidative stress, plants subjected to higher salinity stress showed a considerable build-up in antioxidant levels. As an example, ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were maximally and significantly (p ≤ 0.05) increased by 68, 80, 74, and 58%, respectively, after 400 mM NaCl exposure. The propidium iodide (PI)-stained and NaCl-treated plant roots corroborated the damaging effect of salinity-induced stress on the cell membrane, which was observed under a confocal laser microscope (CLSM). The cells exposed to 400 mM NaCl had maximum fluorescence intensity, indicating that higher level of salts can cause pronounced cell damage and reactive oxygen species (ROS) generation. The increases in superoxide ion (O2–) and hydrogen peroxide (H2O2) content in NaCl-treated plant tissues indicated the elevation of ROS with increasing salt levels. This finding revealed that salt stress can cause toxicity in plants by causing alteration in metabolic activity, oxidative injury, and damage to cell membrane integrity.

Estimating dermal contact soil exposure for amphibians.

Chemical exposure estimation through the dermal route is an underemphasized area of ecological risk assessment for terrestrial animals. Currently, there are efforts to create exposure models to estimate doses from this pathway for use in ecological risk assessment. One significant limitation has been insufficient published data to characterize exposure and to support the selection and parameterization of appropriate models, particularly for amphibians in terrestrial habitats. Recent publications measuring pesticide doses to terrestrial-phase amphibians have begun to rectify this situation. We collated and summarized available measurements of terrestrial amphibian dermal exposure to pesticides from 11 studies in which researchers measured tissue concentrations associated with known pesticide experimental application rates. This data set included tissue concentrations in 11 amphibian species and 14 different pesticides. We then compared the results of two screening exposure models that differed based on surface area scaling approaches as a function of body weight (one based on birds as surrogates for amphibians and another amphibian-specific) to the measured tissue residue concentrations. We define a false-negative rate for each screening model as the proportion of amphibians for which the predicted concentration is less than the observed concentration (i.e., underestimate), contrary to the intent of screening models, which are intended to have a bias for higher exposure concentrations. The screening model that uses birds as surrogates did not have any instances where estimated expected avian doses were less than measured amphibian body burdens. When using the amphibian-specific exposure model that corrected for differences between avian and amphibian surface area, measured concentrations were greater than model estimates for 11.3% of the 1158 comparisons. The database of measured pesticide concentrations in terrestrial amphibians is provided for use in calculating bioconcentration factors and for future amphibian dermal exposure model development.Integr Environ Assess Manag 2023;19:9-16. © 2022 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.

The effect of rising wages of agricultural labor on pesticide application in China

This paper focuses on the issue of how rising wages of agricultural labor affect pesticide application. Using survey data of 3399 rice farmers from 12 provinces in southern China between 2018 and 2019, this paper estimates the effect of wages of agricultural labor on pesticide application by employing the control function (CF) approach. The results suggest that a 10% rise in labor wages corresponds to a 6.88% increase in pesticide dosage and a 4.46% increase in pesticide expenses. Furthermore, this paper analyzes the heterogeneous effects of rising labor wages on pesticide dosage. The results reveal that the positive effect of labor wages on pesticide dosage is weaker for farmers who receive rice subsidies and have cooperative membership but it is stronger for farmers with more rice cultivation experience and those who use more chemical fertilizers. According to these findings, this study proposes policy implications for reducing pesticide application in terms of developing agricultural technologies, encouraging farmers to join cooperatives, and calling on governments to increase crop subsidies.

Degradation of four pesticides in five urban landscape soils: human and environmental health risk assessment

Pesticides are the most cost-effective means of pest control; however, the serious concern is about the non-target effects due to their extensive and intensive use in both agricultural and non-agricultural settings. The degradation rate constant (k) and half-life (DT50) of four commonly used pesticides, glyphosate, 2,4-D, chlorothalonil and dimethoate were determined in five Australian urban landscape soils, with varying physicochemical characteristics, to assess their environmental and human health risks. The k values (day−1) for the selected pesticides were inversely proportional to those of organic carbon (OC), silt, clay and Fe and Al oxides, and directly proportional to pH and sand content in soils. In contrast, the calculated values of DT50 (days) of all the four pesticides in five soils positively correlated with OC, clay, silt and oxides of Fe and Al, whereas soil pH and sand content exhibited a negative correlation. The calculated values of environmental indices, GUS and LIX, for the selected pesticides indicate their potential portability into water bodies, affecting non-target organisms as well as food safety. The evaluation for human non-cancer risk of these pesticides, based on the calculated values of hazard quotient (HQ) and hazard index (HI), suggested that exposure of adults and children to soils, contaminated with 50% of initially applied concentrations, through ingestion, dermal and inhalation pathways might cause negligible to zero non-carcinogenic risks. The present data might help the stakeholders in applying recommended doses of pesticides in urban landscapes and regulatory bodies concerned in monitoring the overall environmental quality and implementing safeguard policies. Our study also clearly demonstrates the need for developing improved formulations and spraying technologies for pesticides to minimize human and environmental health risks.Graphic abstract

Lupeol inhibits pesticides induced hepatotoxicity via reducing oxidative stress and inflammatory markers in rats

The present study was aimed at investigating the toxicity of various pesticides on rat liver. It also aimed to show whether this toxicity could be avoided using lupeol. Adult male Wistars albino rats were randomly divided into nine groups. Control groups were given saline, corn oil, and lupeol; pesticide groups were given malathion, chlorpyrifos, and tebuconazole; in the other three treatments, same doses of pesticides and lupeol were given to the rats for ten days. Histopathological examination showed severe degenerative changes in the pesticide groups. Serum AChE activities, liver GSH, total antioxidant capacity levels, AChE, CAT, SOD, GPx, GR, Na+/K+-ATPase, ARE, and PON were decreased, while serum TNF-α, liver LPO, HP, NO, AOPP, total oxidant status, ROS, and oxidative stress index levels as well as AST, ALT, ALP, GST, arginase and xanthine oxidase activities were increased in the pesticides administered groups. It was observed that the PCNA levels determined by the immunohistochemical method increased in the pesticide groups. Also, the results Raman spectroscopy suggest that the technique may be used to understand/have an insight into pesticide toxicity mechanisms. The administration of lupeol demonstrated a hepatoprotective effect against pesticide-induced toxicity.

Low toxicity crop fungicide (fenbuconazole) impacts reproductive male quality signals leading to a reduction of mating success in a wild solitary bee

Abstract Recent reports on bee health suggest that sublethal doses of pesticides have negative effects on wild bee reproduction and ultimately on their population growth. Females of the solitary horned mason bee Osmia cornuta, evaluate thoracic vibrations and odours of males to assess male quality. When certain criteria are met, the female accepts the male and copulates. However, these signals were found to be modified by sublethal doses of pesticides in other hymenopterans. Here, we tested whether sublethal doses of a commonly used fungicide (Fenbuconazole) impact male quality signals and mating success in O. cornuta. Males exposed to fenbuconazole exhibited reduced thoracic vibrations and an altered cuticular hydrocarbon profile compared to the control bees. Moreover, males exposed to the fungicide were less successful in mating than control males. Synthesis and applications. Our results indicate that a low toxicity fungicide can negatively affect male reproductive success by altering behavioural and chemical cues. This could explain the decreasing pollinator populations in a pesticide‐polluted environment. This study highlights the need for a more comprehensive approach, including behaviour and chemical cues, when testing new pesticides and a more cautionary approach to the pesticides already used on crops.

Exogenous Copper Application for the Elemental Defense of Rice Plants against Rice Leaffolder (Cnaphalocrocis medinalis).

Metals that accumulate in plants may confer protection against herbivorous insects, a phenomenon known as elemental defense. However, this strategy has not been widely explored in important crops such as rice (Oryza sativa L.), where it could help to reduce the use of chemical pesticides. Here, we investigated the potential of copper (Cu) and iron (Fe) micronutrient supplements for the protection of rice against a major insect pest, the rice leaffolder (Cnaphalocrocis medinalis). We found that intermediate levels of Cu (20 μM CuSO4) and high concentrations of Fe (742 μM Fe) did not inhibit the growth of C. medinalis larvae but did inhibit rice root growth and reduce grain yield at the reproductive stage. In contrast, high levels of Cu (80 μM CuSO4) inhibited C. medinalis larval growth and pupal development but also adversely affected rice growth at the vegetative stage. Interestingly, treatment with 10 μM CuSO4 had no adverse effects on rice growth or yield components at the reproductive stage. These data suggest that pest management based on the application of Cu may be possible, which would be achieved by a higher effective pesticide dose to prevent or minimize its phytotoxicity effects in plants.

Contributions on Lindane Degradation by Microcystis aeruginosa PCC 7806

Cyanobacteria are able to tolerate, and even metabolize, moderate doses of organochlorine pesticides, such as lindane (γ-hexachlorocyclohexane), one of the most persistent and widely used in recent decades. Previous work showed that Microcystis aeruginosa PCC 7806 degrades lindane and that, in the presence of the pesticide, microcystin synthesis is enhanced. In this work, using in silico approaches, we have identified in M. aeruginosa putative homologues of the lin genes, involved in lindane degradation in Sphingobium japonicum UT26S. Real-time RT-PCR assays showed that the putative linC gene was induced in the presence of 7 mg/L of lindane. Additionally, prxA, encoding a peroxiredoxin, and involved in oxidative stress response, was also induced when lindane was present. Taking into account these results, M. aeruginosa PCC 7806 may degrade lindane through a metabolic pathway involving a putative 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase encoded by a linC homologue. However, the low similarity of the other potential lin homologues suggest the existence of an alternative pathway different to that of heterotrophic microorganisms such as S. japonicum.