Fly Pests Research Articles

Distribution of Biting Flies Associated with Swine Production Facilities in the Southeastern United States

High populations of pest flies on swine facilities can be detrimental to the animals and producers. The purpose of this study was to identify the distribution pattern and the influence of habitats on distribution of adult biting flies surrounding commercial swine production barns in North Carolina. In this study, fly surveillance was conducted on four swine facilities in Bladen County, North Carolina, U.S.A., from January to October 2019. Biting flies typically associated with livestock were passively collected weekly on sticky traps, which were placed in three concentric circles surrounding swine barns, and then counted and identified. Stable flies [Stomoxys calcitrans (L.); Diptera: Muscidae] were most abundant on swine facilities that were mixed-use with pastured beef cattle. However, swine producers with no pastured cattle should be aware of the potential for stable flies to disperse from off-property larval development sites. Therefore, adult fly management may be necessary if populations reach nuisance levels. Horse and deer fly (Diptera: Tabanidae) captures were not related to trap proximity to water. Differences in horse and deer fly trap captures was likely less of a reflection of differences in potential fly development habitat quality (e.g., water) and more related to adult fly habitat selection.

Integrated control of white fly (Aleurolobus barodensis) of sugarcane at its grand growth stage: A sustainable approach

White fly and other sucking insect pests are considered the minor pests of sugarcane in Punjab-Pakistan. But in the last few years, the white fly of sugarcane has become a major trouble for the sugarcane growers to handle and for sugar industries to extract proper sugar. A field trial was conducted at the seed farm of Kashmir Sugar Mills (Lat. 30.9370, Long. 72.1105). Treatments were comprised of crysopera eggs cards and insecticide(s). The insecticide(s) was sprayed with tractor mounted Jecto sprayer at the grand growth stage of autumn planted sugarcane (8th-month-old crop). Pre and post-spray population counts of whitefly were recorded. The results were compared with the control with a curved polar bar chart in R using the 'ggplot2' package. It was observed that drenching of chlorantraniloprole @ 250 ml/ha made an excellent control (~88%) and 24.2% over the control treatment. The 2nd most efficient control (86%) (22.7% over control) was recorded in a blend of pyriproxyfen + bifenthrin @ 1250 ml + 1000 ml/ha, respectively than the individual use of said insecticide (61% and 69%, respectively). Very hot and dry weather in the month of May in plains of Punjab Province-Pakistan did not support the Lab. reared crysoperla eggs cards @ 15000/ha in the field. But a large number of natural bio control agents worked well and surpassed some treatments in % mortality of whitefly

HCN-induced embryo arrest: passion fruit as an ecological trap for fruit flies.

Fruit flies are important economic pests of fruits, vegetables, and nuts all over the world. In this study, a permanent ecological trap, which was created by the ovicidal effect of phytogenic hydrogen cyanide (HCN) liberated from passion fruits due to oviposition by fruit flies and can be used in the pest management, were determined. Observation of fruit fly eggs in Passiflora within the passion fruit cultivation region in southern China, from Aug 2019 to Oct 2020 showed that the exotic Passiflora attracted the native fruit flies to oviposit, but the eggs could not hatch. Using classical staging to categorize embryonic development and fumigation assays, we show that oviposition by fruit fly on passion fruits, release HCN from the cyanogenic mesocarp. Exposure of the eggs to HCN causes arrest of embryonic development and finally the death of eggs. Our results reveal that the life cycle of fruit fly in Passiflora is interrupted at the egg stage. Consequently, we predict that this ecological trap may be permanent. Extensive cultivation of the Passiflora vine as a dead-end trap crop may be an effective avenue to reduce populations of fruit fly pests. © 2023 Society of Chemical Industry.

Comparative demography of a specialist and generalist fruit fly: Implications for host use and pest management

AbstractHost plants used by phytophagous insects can have significant consequences on demography parameters, overall lifetime fitness and their subsequent population dynamics. Here, we conduct a comparative demographic study between the specialist Zeugodacus cucumis (French) and generalist Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) to determine whether the host plants used by these fly species play any role in their overall lifetime fitness and explains current host use patterns. These two fly species are pests within the north‐eastern region of Australia and we further aimed to use complete life‐history data to determine the population parameters and models that would help identify the sensitive life‐history stage that could be targeted for effective field management. Eggs collected from laboratory‐reared flies were inoculated into organically grown fruits of both the primary and alternate host plant cultivars of both fly species. The proportion surviving each life stage from egg through to adult and fecundity were monitored for all cohorts from the different plant cultivars. Complete stage‐base life‐tables for cohorts of each fly species developing from each fruit cultivar were constructed, and the key demographic parameters and population models were analysed using PopTools matrix model programme. Our results showed that the host used by each fly species had significant consequences on fly demographic parameters and hence their overall lifetime fitness. The generalist B. tryoni was able to compensate for the fitness loss experienced at the pre‐adult stage by having adults with higher fecundity, but this was not the case for the specialist Z. cucumis. Stage‐base population models revealed that the population growth rate of both species was highly sensitive at the adult reproductive stage, indicating that manipulating probability of survival at this life stage would effectively manage populations of these pest species. This study provides the empirical evidence of undertaking complete life history demography studies of phytophagous insects to accurately understand their lifetime fitness consequences of using a certain host, their observed host use patterns, and overall population dynamics. We suggest that any efforts to manage dacine fruit fly pest population should consider life‐history consequences of host use.

Genetic diversity and organic waste degrading capacity of Hermetia illucens from the evergreen forest of the Equatorial Choco lowland.

Globally, microplastics (MP) represent a growing burden for ecosystems due to their increasing presence at different trophic levels. In Ecuador, the lack of waste segregation has increased the quantity of waste, primarily organics and plastics, overloading landfills and water sources. Over time, plastics reduce in size and silently enter the food chain of animals, such as insects. The black soldier fly (BSF) larvae, Hermetia illucens (Linnaeus, 1758), is a species with devouring behavior used for waste management because of its beneficial qualities such as fly pest control, biomass production, and rapid organic waste degradation. Studies have uncovered the insect's ability to tolerate MP, and consider the possibility that they may be able to degrade polymers. For the first time in Ecuador, the present study characterized H. illucens using the sequences of different molecular markers. Finally, H. illucens' degrading capacity was evaluated in the presence of MP and decaying food residues, resembling landfill conditions.

Efektivitas Beberapa Jenis Feromon Organik Sebagai Atraktan Lalat Buah Pada Tanaman Cabai

Red chili (Capsicum annuum L.) is a commodity that has high economic value, but the potential loss experienced in chili cultivation is also quite high, because chili is quite vulnerable to fruit fly pests. This study aims to determine the response of fruit flies to the use of pheromones derived from various kinds of fruit peels and flesh added with yeast (organic pheromones). This research took place on chili farmers' land located in the village of Tambak Langsat, West Ulin Platform, Banjarbaru City. This study used a randomized block design (RBD) with 11 treatments including the control. The results showed that the treatment given organic pheromones was able to trap fruit flies. Of all the pheromones that were most effective at getting lots of fruit flies was the chili pheromone (0.2 ml) with a catch of 25 individuals and for organic pheromones that were less effective was the mango pheromone (0.2 ml) with a catch of 4 individuals. The type of fruit fly B. dorsalis dominates of the four species with the Diversity Index (H´) of fruit flies being classified as moderate, namely 1.1082, the Dominance Index (D) being high 1 and for fruit flies dominating B. dorsalis.

Comparison of Methyl Bromide and Ethyl Formate for Fumigation of Snail and Fly Pests of Imported Orchids.

Invasive snails and flies are major pests of imported orchids, controlled by methyl bromide (MB) fumigation in Korea. We compared the efficacy and phytotoxicity of ethyl formate (EF) and MB on four species of imported orchids using juvenile stages of Achatina fulica and third and fourth instars of Lycoriella mali. EF was as effective as MB. The LCt99 values of EF were 68.1 and 73.1 g h/m3 at 15 °C; and those of MB were 95.9 and 78.4 g h/m3 at 15 °C for A. fulica and L. mali, respectively. In the scale-up trials, EF treatment at 35 g/m3 for 4 h at 15 °C resulted in complete control of both pests. MB treatment based on the current treatment guidelines for imported orchids (48 g/m3, 2 h, at >15 °C) resulted in complete control of L. mali but not of A. fulica. Chlorophyll content and hue values of treated orchids were not affected by EF treatment but significantly changed by MB (p-value < 0.05). All four treated species of orchids died within 30 d of MB treatment, while only one species died from EF treatment. Our results suggest that EF is a potential alternative to MB in phytosanitary treatment of imported orchids.

Effect of Application Intervals of Various Types of Insecticides in Controlling Fruit Fly (Bactrocera sp.) Pests on Production of Curly Red Chili (Capsicum annum L.)

The study's full title was "The Effect of Application Intervals of Different Types of Insecticides in Controlling Fruit Fly (Bactrocera sp.) Pests on Production of Curly Red Chili (Capsicum annum L.)." From July 2021 to October 2021, it was conducted in the dormitory area of the Riau Islamic University. The goal of this study was to ascertain how different vegetable insecticide kinds interacted with treatment intervals to control fruit fly pests and curly red chili plant production. This study employed a two-factor, entirely randomized design. The first factor was a combination of four different doses of pesticides, including isoprocarb 2 g/l, betel leaf extract 100 cc/l, neem leaf extract 100 cc/l, and lime leaf extract 100 cc/l. The application time interval, which has four levels—3 days, 6 days, 9 days, and 12 days—is the second element. 48 experimental units were created by combining 16 treatment combinations, each of which had three replications. There were 192 plants total because there were 4 plants per experimental unit and 2 plants were used as samples. The data is statistically analyzed before being subjected to the BNJ test. The outcomes demonstrated that not all parameters were affected by interactions and combinations of application intervals and different insecticide types, including the percentage of flowers to fruit, age at first harvest, fruit weight per plant, fruit weight per fruit, percentage of fruit flies attacked, other types of pests that attack, and age of disease across all treatments.

Tri-trophic interactions among Fopius arisanus, Tephritid species and host plants suggest apparent competition.

When several polyphagous herbivore species share a parasitoid, the tri-trophic interaction networks can be difficult to predict. In addition to direct effects, the parasitoid may influence the herbivore community by mediating indirect interactions among hosts. The plant species can also modulate the parasitoid preference for a specific host. One of the indirect effects is apparent competition, a negative interaction between individuals as a result of the action of shared natural enemies. Here, we focus on the interactions between the parasitoid Fopius arisanus (Braconidae) and two generalist fruit fly pests: Bactrocera dorsalis and Bactrocera zonata (Tephritidae). This parasitoid was introduced into La Réunion in 2003 to control populations of B.zonata and can also interact with B.dorsalis since its invasion in 2017. Our main objective is to characterize the tri-trophic interactions between F.arisanus, fruit fly and host plant species. We developed a long-term field database of fruit collected before and after the parasitoid introduction and after the B.dorsalis invasion in order to compare parasitism rate and fruit fly infestation for the different periods. In laboratory assays, we investigated how the combination of fruit fly species and fruit can influence the preference of F.arisanus. In the field, before the invasion of B.dorsalis, the parasitism rate of F.arisanus was low and had a little impact on the fruit fly infestation rate. After the B.dorsalis invasion, we observed an increase in parasitism rate from 5% to 17%. A bioassay showed that females of F.arisanus could discriminate between eggs of different fruit fly and host plant species. The host plant species preference changed in relation to the fruit fly species inoculated. Field observations and laboratory experiments suggest the possible existence of apparent competition between B.dorsalis and B.zonata via F.arisanus.

JENIS DAN INTENSITAS KERUSAKAN HAMA UTAMA TANAMAN CABAI PADA SISTEM BUDIDAYA YANG BERBEDA

The research aims to determine the main types of pests and the intensity of their damage to chili plants with different cultivation systems. This research was conducted in Konda Satu Village, Konda District, South Konawe Regency. This study consisted of 3 treatments with 6 replications, so there were 18 experimental units. Observational data were analyzed using analysis of variance (ANOVA) and if the results showed significant differences then it was continued with the Least Significant Difference Test (LSD) at the 95% confidence level to find out the best treatment by testing the differences between all treatments. Then test further LSD0.05. This study used three cultivation systems as treatments, namely the Conventional Cultivation System (Without Application of Biofresh Fertilizer + 100% Inorganic recommended dose + Use of Plastic Mulch), Semi-Organic Cultivation System (Application of Biofresh Fertilizer 50% recommended dose + 50% Inorganic of recommended dose + Application Phymar C Botanical Pesticides + Use of Plastic Mulch), and Organic Cultivation Systems (100% recommended dosage of Biofresh Fertilizer Application + Phymar C Botanical Pesticide Application + Organic Mulch). The results showed that the main pests that attack chili plants are armyworm pests (Spodoptera litura), fruit fly pests (Bactrocera sp.) and Aphids pests (Aphididae). The lowest damage intensity of armyworm pests was in the organic cultivation system of 18.08%, the lowest intensity of fruit fly pest attacks was in the semi-organic cultivation system of 31.81%, the lowest intensity of attack by aphids was in the semi-organic cultivation system of 12.59%.

Larval nutritional-stress and tolerance to extreme temperatures in the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae)

ABSTRACTWithin the factors affecting insect tolerance to extreme environmental conditions, insect nutrition, particularly of immature stages, has received insufficient attention. In the present study, we address this gap by investigating the effects of larval nutrition on heat and cold tolerance of adult Bactrocera zonata – an invasive, polyphagous fruit fly pest. We manipulated the nutritional content in the larval diet by varying the amount of added yeast (2–10% by weight), while maintaining a constant sucrose content. Adults derived from the different larval diets were tested for their tolerance to extreme heat and cold stress. Restricting the amount of yeast reduced the efficacy of the larval diet (i.e. number of pupae produced per g of diet) as well as pupal and adult fresh weight, both being significantly lower for yeast-poor diets. Additionally, yeast restriction during the larval stage (2% yeast diet) significantly reduced the amount of protein but not lipid reserves of newly emerged males and females. Adults maintained after emergence on granulated sugar and water for 10 days were significantly more tolerant to extreme heat (i.e. knock-down time at 42 oC) when reared as larvae on yeast-rich diets (8% and 10% yeast) compared to counterparts developing on a diet containing 2% yeast. Nevertheless, the composition of the larval diet did not significantly affect adult survival following acute cold stress (exposure to −3°C for 2 hrs.). These results are corroborated by previous findings on Drosophilid flies. Possible mechanisms leading to nutrition-based heat-tolerance in flies are discussed.

Transcriptome-Based Selection and Validation of Reference Genes for Gene Expression in Goji Fruit Fly (Neoceratitis asiatica Becker) under Developmental Stages and Five Abiotic Stresses.

Goji fruit fly, Neoceratitis asiatica, is a major pest on the well-known medicinal plant Lycium barbarum. Dissecting molecular mechanisms of infestation and host selection of N. asiatica will contribute to the determination of best management practices for pest fly control. Gene expression normalization by Real-time quantitative PCR (qPCR) requires the selection and validation of appropriate reference genes (RGs). Hence, 15 candidate RGs were selected from transcriptome data of N. asiatica. Their expression stability was evaluated with five algorithms (∆Ct, Normfinder, GeNorm, BestKeeper, and RefFinder) for sample types differing in the developmental stage, sex, tissue type, and in response to five different abiotic stresses. Our results indicated that the RGs β-Actin + GST for sex, RPL32 + EF1α for tissue type, RPS13+ EF1α for developmental stages along with odor stimulation, color induction, and starvation-refeeding stresses, EF1α + GAPDH under insecticide stress, RPS13 + RPS18 under temperature stress, respectively, were selected as the most suitable RGs for qPCR normalization. Overall, RPS18 and EF1α were the two most stable RGs in all conditions, while RPS15 and EF1β were the least stable RGs. The corresponding suitable RGs and one unstable RG were used to normalize a target odorant-binding protein OBP56a gene in male and female antennae, different tissues, and under odor stimulation. The results of OBP56a expression were consistent with transcriptome data. Our study is the first research on the most stable RGs selection in N. asiatica, which will facilitate further studies on the mechanisms of host selection and insecticide resistance in N. asiatica.

Pannier is a key regulator of embryogenesis, pupal development and female reproduction in the insect pest Bactrocera dorsalis.

Most arthropods are famous for their large reproductive capacity, with the ovary playing a vital role in the process. The study of the regulatory mechanisms of ovarian development may have the potential for a reproduction-based pest management strategy. GATA-binding transcription factors (GATAs) as important regulatory factors mediate many physiological processes, including development, immunity, insecticide resistance and reproduction. The Pannier (pnr), a member of GATA family, was confirmed to be involved in ovarian development of Bactrocera dorsalis in our previous study. However, the direct evidence of pnr regulating the fly ovarian development is still lacking. We used CRISPR/Cas9 to create Bdpnr loss-of-function mutations. Homozygous Bdpnr-/- mutants were nonviable, with most individuals dying during embryogenesis, some surviving to the larval stages, and the remaining few dying during pupation. In contrast, heterozygous individuals reached the adult stage, but ovarian development was disrupted, with concomitant decreases in egg laying and hatching rates. We also found that two genes encoding vitellogenin proteins (BdVg1 and BdVg2) and the vitellogenin receptor (BdVgR) were significantly down-regulated in heterozygous mutants compared to wild-type controls. These results indicate that Bdpnr is required for embryonic and post-embryonic development, including the formation of ovaries. Bdpnr could therefore be considered as a molecular target for tephritid fly pest control. © 2022 Society of Chemical Industry.

Bactrocera dorsalis in the Indian Ocean: A tale of two invasions.

An increasing number of invasive fruit fly pests are colonizing new grounds. With this study, we aimed to uncover the invasion pathways of the oriental fruit fly, Bactrocera dorsalis into the islands of the Indian Ocean. By using genome-wide SNP data and a multipronged approach consisting of PCA, ancestry analysis, phylogenetic inference, and kinship networks, we were able to resolve two independent invasion pathways. A western invasion pathway involved the stepping-stone migration of B.dorsalis from the east African coast into the Comoros, along Mayotte and into Madagascar with a decreasing genetic diversity. The Mascarene islands (Reunion and Mauritius), on the contrary, were colonized directly from Asia and formed a distinct cluster. The low nucleotide diversity suggests that only a few genotypes invaded the Mascarenes. The presence of many long runs of homozygosity (ROH) in the introduced populations is indicative of population bottlenecks, with evidence of a more severe bottleneck for populations along the western migration pathway than on the Mascarene islands. More strict phytosanitary regulations are recommended in order to prevent the further spread of B.dorsalis.

Rapid and transient evolution of local adaptation to seasonal host fruits in an invasive pest fly.

Both local adaptation and adaptive phenotypic plasticity can influence the match between phenotypic traits and local environmental conditions. Theory predicts that environments stable for multiple generations promote local adaptation, whereas highly heterogeneous environments favor adaptive phenotypic plasticity. However, when environments have periods of stability mixed with heterogeneity, the relative importance of local adaptation and adaptive phenotypic plasticity is unclear. Here, we used Drosophila suzukii as a model system to evaluate the relative influence of genetic and plastic effects on the match of populations to environments with periods of stability from three to four generations. This invasive pest insect can develop within different fruits, and persists throughout the year in a given location on a succession of distinct host fruits, each one being available for only a few generations. Using reciprocal common environment experiments of natural D.suzukii populations collected from cherry, strawberry, and blackberry, we found that both oviposition preference and offspring performance were higher on medium made with the fruit from which the population originated than on media made with alternative fruits. This pattern, which remained after two generations in the laboratory, was analyzed using a statistical method we developed to quantify the contributions of local adaptation and adaptive plasticity in determining fitness. Altogether, we found that genetic effects (local adaptation) dominate over plastic effects (adaptive phenotypic plasticity). Our study demonstrates that spatially and temporally variable selection does not prevent the rapid evolution of local adaptation in natural populations. The speed and strength of adaptation may be facilitated by several mechanisms including a large effective population size and strong selective pressures imposed by host plants.

Schedule Making and Implementation of Preventive Maintenance on Fruit Fly Pest Catching Equipment

Schedule Making and Implementation of Preventive Maintenance on Fruit Fly Pest Catching Equipment

Competition: A Missing Component of Fruit Fly (Diptera: Tephritidae) Risk Assessment and Planning.

Tephritid fruit flies are internationally significant pests of horticulture. Because they are also highly invasive and of major quarantine concern, significant effort is placed in developing full or partial pest risk assessments (PRAs) for fruit flies, while large investments can be made for their control. Competition between fruit fly species, driven by the need to access and utilise fruit for larval development, has long been recognised by researchers as a fundamental component of fruit fly biology, but is entirely absent from the fruit fly PRA literature and appears not be considered in major initiative planning. First presenting a summary of the research data which documents fruit fly competition, this paper then identifies four major effects of fruit fly competition that could impact a PRA or large-scale initiative: (i) numerical reduction of an existing fruit fly pest species following competitive displacement by an invasive fruit fly; (ii) displacement of a less competitive fruit fly pest species in space, time or host; (iii) ecological resistance to fruit fly invasion in regions already with competitively dominant fruit fly species; and (iv) lesser-pest fruit fly resurgence following control of a competitively superior species. From these four major topics, six more detailed issues are identified, with each of these illustrated by hypothetical, but realistic biosecurity scenarios from Australia/New Zealand and Europe. The scenarios identify that the effects of fruit fly competition might both positively or negatively affect the predicted impacts of an invasive fruit fly or targeted fruit fly control initiative. Competition as a modifier of fruit fly risk needs to be recognised by policy makers and incorporated into fruit fly PRAs and major investment initiatives.

Sexual competitiveness of sterile Ceratitis capitata males exposed to essential oils from non‐host plant species native to Argentina

AbstractThe sterile insect technique (SIT) is used to control fruit fly pests, such as the Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Enhancing sexual competitiveness of mass‐reared sterile males can contribute to making this technique more effective. It has been shown that exposure to volatiles from essential oils (EOs), such as ginger root oil (GRO) and those from host fruits, increases male mating success. We evaluated the effect of EOs from non‐host species native to Argentina, Schinus polygama (Cav.) Cabrera (Anacardiaceae) and Baccharis spartioides (Hook. & Arn.) Remy (Asteraceae), on the sexual competitiveness of sterile C. capitata males. In field cage experiments, sterile males exposed to S. polygama EO increased their mating success. In addition, sterile males exposed to this EO achieved more matings on trees than non‐exposed males, thus suggesting the former are more prone to locate and defend the pheromone‐calling territory. Deprivation of water and/or food significantly reduced males' life span, but exposure to S. polygama EO and GRO did not affect their survival. Schinus polygama EO is composed of mono‐ and sesquiterpenes with behavioral and electroantennographic responses in medflies, indicating that semiochemicals that boost medfly sexual competitiveness combine in this EO.

Analysis of pest flies in cattle and their effect on behavioural response in cattle

India is an agriculture-based county. People in India mostly practices mixed farming. Mixed farming constitutes farming along with rearing of livestock. Animal husbandry is a significant source of income for many farmers in India. Numerous vertebrates, including domestic animals such as cows, are severely harmed by ectoparasites, resulting in anaemia, weakness, and even mortality of the host animal. Among the ectoparasites, flies are important carriers of diseases including trypanosomiasis and the bovine leucosis virus, among others. Bovine Leukaemia Virus which is spread by horn flies, is the most prevalent neoplastic illness in cattle. Ectoparasitic infection is the major threat to the maintenance and health of live cattle. Hence, the present study was conducted to analyse some pest flies in cattle and their effect on behavioural response in cattle of the Gavandgaon village of Degloor taluka of Nanded district (M.S.) during July 2022 to September 2022. In this study a total 16 cattle were physically observed to study different pest flies in cattle and their impact on behavioural response in cattle.

Double minute chromosomes in Anastrepha fraterculus (Wiedemann) (DIPTERA: Tephritidae): a model for cancer studies

Anastrepha fraterculus (Wied.) is known as the South American Fruit Fly. In order to develop efficient control strategies, we need deep knowledge on its biology along with periodical studies on population dynamics. Citogenetic studies on natural populations of this pest fly made it possible to reveal the presence of double minute chromosomes (DMs) in several natural populations of this insect. Cytogenetic studies performed in our insect laboratory, allowed the genetic analysis through a genealogical methodology. The establishment of families made it possible the genetic studies allowing the rigorous identification, characterization and confirmation of new chromosomal variants, frequently missinterpreted when the materials analyzed only come from nature. Double minute chromosomes -considered a type of chromosomal rearrangement- are extra chromosomal gene copies. This study sought to answer: What role do DMs chromosomes play in laboratory populations of the pest derived from natural populations? How are they transmitted from one generation to another? To answer our questions, we analyzed for 25 generations, two laboratory populations derived from Tucuman (T) and Buenos Aires (BA -tester) guava,. Data were recovered from cytological analysis of ganglia preparations revealed with H33258. The natural population from BA didn’t carry DMs. Results showed DMs in flies of both laboratory populations. DMs were transmitted from parents to progenies through 25 generations and their transmission was randomized in number. DMs are the cytological expression of resistance mechanisms used by the pest as a response to environmental stress. DMs in BA strain marked the change to the laboratory environment. A. fraterculus is a model insect for the study of cancer.