In recent years, nanozymes have increasingly attracted the attention of researchers due to their low cost, high stability, and straightforward preparation when compared with natural enzymes. Furthermore, the biocompatibility of nanozymes has been enhanced through surface adsorption, hence broadening their application scope. In this work, Co3O4 nanozyme was adsorbed with diverse DNA, and the catalytic performance was significantly boosted compared with the pure Co3O4 nanozyme. Peroxidase-like activity assays revealed three key findings. First, the adsorption of single-stranded DNA proved to be more effective than that of double-stranded DNA. Second, the 50-nucleotide single-stranded DNA sequence yielded the stronger catalytic signal. Ultimately, guanine rich nucleotide exhibited the higher catalytic efficiency in single-stranded DNA and cytosine-guanine rich nucleotide exhibited the stronger catalytic efficiency in double-stranded DNA. The kinetic results of its catalytic activity showed that the catalysis of the adsorbed Co3O4 nanozyme followed the Michaelis-Menten kinetics. In parallel, the Km value of 50-nucleotide single-stranded DNA adsorption was the lowest of diverse DNA, which was 6.11 mM and 54.8% of the pure Co3O4 nanozyme. These findings establish DNA adsorption as an effective strategy for enhancing the catalytic activity of Co3O4 nanozymes, presenting significant potential for applications in medical diagnostics and antioxidant-related fields.

Nanoarchitectured biomass-waste derived activated charcoal nanozymes and its application in visual analysis of nitrite in pickled food.

Multi-functional Fe-metallohydrogel with peroxidase/oxidase activities for dye degradation and nitrite detection.

Near infrared light-enhanced peroxidase-like catalytic activity of copper tannic acid nanosheets for ultrasensitive detection of volatile sulfur compounds.

Bioinspired amino acid-functionalized carbon dots nanozymes with enhanced peroxidase-like activity for sensitive detection of GSH in food.

One fell swoop strategy to detect and degrade malathion by an ALP-sensitive and Pt-Co-N-C nanozyme-enhanced chemiluminescent 3D μPAD.

Colorimetric detection of tetracycline by enhancing peroxidase-like activity of polymerized MnO2@ZIF-67 nanocomposites.

Synergistic self-calibration strategy based on nano-cornucopia MOFs for accurate HER-2 detection in precision breast cancer diagnosis.

Pyrococcus furiosus Argonaute biosensing platform based on Hemin@ZIF-90 nanozyme-modulated signal amplification for sensitive detection of Salmonella typhimurium.

Multifunctional nanozyme-embedded hydrogels for advanced diabetic wound management.

Dual enzyme-mimicking bimetallic MOF for selective SERS detection of L-DOPA in human serum based on cascade catalytic reaction.

Green synthesis of hawthorn polysaccharide-stabilized Pd@Ru nanozymes for sensitive colorimetric detection of organophosphorus pesticides.

Colorimetric sensor array utilizing copper based MOF composite with peroxidase-like and laccase-like dual-enzyme mimetic activities for discrimination of multiple pesticides

Pt nanoparticles modified single-atoms nanozymes with tunable metal valence for rapid chlorpyrifos colorimetric detection.

Microfluidic engineering of AgNPs@ZIF-8 composites enables tunable peroxidase-like catalytic activity for enhanced bacterial inactivation

Ultrasensitive photoelectrochemical biosensor of amyloid-β oligomer detection via porphyrin-based covalent organic frameworks enhanced by iron single-atom catalysts.

Advancing sustainable graphene production is crucial to unlocking its potential in biomedicine and environmental technology. Although graphene offers exceptional electrical, mechanical, and surface properties, its large-scale utilisation is hindered by conventional synthesis methods such as chemical vapour deposition (CVD), which are costly, energy-intensive, and environmentally harmful. In this study, we report an eco-friendly, scalable, and cost-effective protein-assisted approach for exfoliating graphite into high-quality graphene in aqueous media using bovine serum albumin (BSA) as a biodegradable dispersant. BSA not only enables efficient exfoliation but also introduces abundant protein-derived functional groups, improving aqueous stability (zeta potential: -28.8 mV at pH 8) and ensuring long-term colloidal dispersion. These moieties enhance dispersibility while imparting intrinsic peroxidase-like activity under acidic conditions. Utilizing this nanozymatic activity, we developed a colorimetric sensing platform for rapid and sensitive detection of oxytetracycline (OTC). The assay is based on the inhibition of biographene-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of hydrogen peroxide, where OTC suppresses radical-mediated oxidation. The sensor exhibited high selectivity with a low detection limit of 2.66 µM and showed good biocompatibility, underscoring its biomedical potential. The green strategy enables sustainable production of functional graphene, positioning biographene as a promising material for diverse sensing applications.

Atomic-shell engineering in manganese-doped palladium@iridium nanozymes: d-Band optimization for enhanced peroxidase-like activity in ultrasensitive lateral flow immunoassays

Oxygen vacancies rich yttrium doped-Co3O4 nanosheets with peroxidase-like activity for sensitive rutin detection

Ratiometric fluorescent aptasensor for determination of Golgi Protein 73 based on boron, nitrogen co-doped carbon quantum dots and copper metal-organic framework.