The objective of this study was to examine the chemical composition of essential oil A. indica leaves and its nanoemulsion, and evaluation of potential for use as a biopesticide. Essential oil of A. indica extracted from fresh leaves was found to be 99.97% composed of 59 components, according to GC-MS analysis. A non-ionic surfactant Tween 80 and the essential oil of A. indica were combined to create a stable nanoemulsion using sonication method. A long durable stable nanoemulsion was obtained after thirty minutes of sonication, and it was examined using TEM (Transmission electron microscopy), DLS (Dynamic light scattering), and FTIR (Fourier transform infrared spectroscopy) analyses. To consider its practical applications, other factors such as stability, viscosity, pH, turbidity, and creaming index were also investigated. Antibacterial properties of essential oil and nanoemulsion were evaluated against bacterial blight plant pathogens Xanthomonas oryzae and Xanthomonas citri. The MIC (Minimum inhibitory concentration) was determined using the broth microdilution method, and the anti-Xanthomonas activity was assessed utilizing by agar disc diffusion method. Using the crystal violet (CV) technique, inhibition of biofilm formation and development were evaluated. Disturbance of bacterial membrane permeability was confirmed by estimating the proteins and nucleic acids (DNA, and RNA) leakage from the cell membrane. Greenhouse studies using rice/paddy plants were conducted to demonstrate the antibacterial potential of essential oil and nanoemulsion, using RNR-15048 rice cultivar by seed treatment and foliar spray groups. Results of the investigations suggest that, when the essential oil was transformed into a nanoemulsion, its antibacterial effect against bacteria was significantly increased, that might be attributed to the essential oil enhanced ability to penetrate the bacterial cells. Essential oil of A. indica and its nanoemulsion reduced rice bacterial blight disease by 84%and pathogen-virulence by 92%, and promoted the growth and development of rice/paddy seedlings. These results confirm that A. indica essential oil and its nanoemulsion possess anti-bacterial blight properties, without affecting growth and development of host plants. Essential oil of A. indica and its nanoemulsion could be used as biopesticide to control Xanthomonas induced bacterial blight diseases in crop plants either by using for seed treatment or as a foliar spray.