Dormant Period Research Articles

МЕТОДОЛОГИЯ УПРАВЛЕНИЯ АГРОБИОЛОГИЧЕСКОЙ, АДАПТИВНОЙ И ПРОДУКЦИОННОЙ УСТОЙЧИВОСТЬЮ НАСАЖДЕНИЙ ВИНОГРАДА В НЕСТАБИЛЬНЫХ ПОГОДНЫХ УСЛОВИЯХ И ТЕХНОГЕННОЙ ИНТЕНСИФИКАЦИИ ПРОИЗВОДСТВА

The article presents the results of agrobiological and physiological-biochemical studies to ensure the stability of grape plantations in unstable weather conditions and anthropogenic intensification of production. Studies in the agroecological conditions of the Krasnodar region on the southern chernozems revealed the most productive norm of loading of grape bushes with shoots for the technical varieties Kurchanskiy, Dmitriy, Vladimir, Granatovyi, Antaris and Alkor, on leached chernozems – for the table seedless variety Centennial Seedless. According to the results of electrophoretic separation of peroxidases in polyacrylamide gel and an increased content of ascorbic acid, increased frost resistance was established in the varieties Kristall, Vostorg, Krasnostop AZOS. The Vostorg and Zarif varieties proved to be more adaptive to the stresses of the summer period in terms of leaf hydration, bound water content, the ratio of chlorophylls and carotenoids, the amount of proline and water-soluble sugars, membrane permeability. The role of physiologically active substances in increasing the adaptive potential of grape plants has been established. The treatment of Merlot vines with methyl jasmonate and epibrassinolide during deep dormancy period, as well as proline, salicylic acid and epibrassinolide during induced dormancy period had a positive effect on the condition of the vine: there was a decrease in electrolyte yield. Sustainable grape cultivation is achieved with the systemic use of the «Bioconcentrate-Z» effluent by the non-root method. Against the background of nonroot treatments of Merlot and Cabernet Sauvignon varieties, a higher level of plant water supply, an increase in potassium content, green pigments, organic acids and grape yields were established. Physiologically active compounds and the preparation Turinbash have a positive effect in intensive own-root plantations of native and promising introduced grape varieties against the background of phylloxera infection in the southern part of Dagestan. The created yield forecasting method based on the NDVI of the grape plant and the actual data of multispectral images of the phenophases of flowering and growth allows to increase the accuracy of the calculated yield capacity to the actual one with a deviation from 0 to 0.8 kg per bush, the accuracy of the forecast increases by 2 times by the end of the growth phase and by the beginning of the maturation phase. Key words: GRAPES, METHODS, MANAGEMENT, SUSTAINABILITY, PRODUCTIVITY, QUALITY, FORECASTING

Dynamics of alkaloid accumulation in Narcissus cv.Hawera: a source of Sceletium-type alkaloids.

The Sceletium-type alkaloids, known for their anxiolytic and antidepressant activities, have been recently found to be biosynthesized in Narcissus cv. Hawera, which is largely used as an ornamental plant. An alkaloid fraction enriched with Sceletium-type alkaloids from the plant has shown promising antidepressant and anxiolytic activities. In the present study, qualitative and quantitative analyses of the alkaloids in the plant organs were performed during one vegetation season by GC-MS. The alkaloid pattern and total alkaloid content was found to depend strongly on the stage of development and plant organ. The alkaloid content of bulbs was found to be highest during the dormancy period and lowest in sprouting bulbs. The leaves showed the highest alkaloid content during the intensive vegetative growth andlowest during flowering. In total, 13 alkaloids were detected in the methanol extracts of Narcissus cv. Hawera, six Sceletium-type and seven typical Amaryllidaceae alkaloids. Major alkaloids in the alkaloid pattern were lycorine, 6-epi-mesembrenol, mesembrenone, sanguinine, and galanthamine. The leaves of flowering plants were found to have the highest amount of 6-epi-mesembrenol. Mesembrenone was found to be dominant alkaloid in the leaves of sprouting bulbs and in the flowers. Considering the biomass of the plant, the dormant bulbs are the best source of alkaloid fractions enriched with 6-epi-mesembrenol. Theflowers and the young leaves can be used for preparation of alkaloid fractions enriched with mesembrenone. Theresults indicates that Narcissus cv. Hawera is an emerging source of valuable bioactive compounds and its utilization can be extended as a medicinal plant.

Abstract 5386: Increased sprouting angiogenesis in a microphysiological model of cisplatin-resistant recurrent lung cancers

Abstract Non-small cell lung cancer (NSCLC) constitutes 85% of total lung cancer cases and first-line therapies still often employ the platinum-based agents. Cisplatin therapy for NSCLC often leads to relapse of aggressive tumors with high metastatic potential after a period of dormancy. We hypothesize that cisplatin-resistant tumor cell phenotypes will increase features of aggressivity including sprouting angiogenesis in microphysiological models of NSCLC. First, we engineered various TME configurations using A549 cell spheroids and cancer associated fibroblasts (CAF) to establish a baseline of tumor cell proliferation, ECM deposition, and expression of proangiogenic and proinflammatory genes. Next, we generated cisplatin-resistant (Cis-R) A549 sub-lines to test the hypothesis that 3D tumor models engineered using these drug-resistant cells will capture hallmarks of highly aggressive recurrent tumors such as increased expression of progression-associated genes, increased induction of local stromal reactions and more profound angiogenesis. We treated parent A549 with 25 μM cisplatin for 96 hours followed by at least 14 days of recovery to generate Cis-R A549 cells. Interestingly, Cis-R spheroids exhibited significantly lower rates of proliferation (P<0.01) relative to parent A549 spheroids as measured by Ki67 index. Cis-R cells and spheroids exhibit increased genetic expression for genes involved in inflammation, EMT, proliferation and chemoresistance. Furthermore, Cis-R cells in 2D displayed a mesenchymal-like morphology and further exhibited dysmorphic spheroid morphology in 3D. We then developed a membrane free microphysiological system (MPS) comprised of two adjacent and contiguous tissue layers to investigate cancer mediated angiogenesis. We seeded one layer with lung fibroblasts and endothelial cells, which formed a vascularized network, while the other lane was cultured with no cells (control), parent A549 spheroids, or Cis-R A549 spheroids. Media void of VEGF was used throughout the duration of the experiment to isolate the effect of TME-derived factors. We measured increased angiogenetic sprouting induced by Cis-R tissues relative to parent using the following metrics: sprout density (Cis-R = 49.86 ± 10.93 µm/µm2, parent = 13.73 ± 4.12 µm/µm2, blank = 14.76 ± 4.22 µm/µm2), sprout length (Cis-R = 15006 ± 5507 µm, parent = 2466 ± 639.1 µm, blank = 4749 ± 1547 µm), and sprout area fraction (Cis-R = 0.10 ± 0.028, parent = 0.041 ± 0.010, blank = 0.032 ± 0.0095). Thus, the creation of drug resistant tumor cell sub lines enables the construction of more aggressive engineered tumor microenvironments. Future iterations of our MPS model will be used to investigate angiogenesis in the context of additional cancers, including breast and colon, while transitioning to the use of patient-derived organoids. Citation Format: Elisabet Olsen, G. Wills Kpeli, Omar M.K. Ahmad, Mark Mondrinos. Increased sprouting angiogenesis in a microphysiological model of cisplatin-resistant recurrent lung cancers [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 5386.

Abstract 515: Endocycling cancer cells undergo multiple S phases while repressing myc activity

Abstract Chemotherapy resistance remains a major challenge when treating patients with metastatic cancer. While intrinsic resistance to therapy has been well studied, phenotypic plasticity that drives entry and exit of resistant cell states is poorly understood. One of these plastic cell states used by cancer cells under chemotherapy treatment is characterized by the reprogramming of the mitotic cell cycle to enter a non-proliferative endocycle. Cells in this endocycling state decouple DNA synthesis from cell division by actively progressing through G1, S, and G2 phases of the cell cycle while repeatedly skipping mitosis. After a dormant period, cells can switch back to a mitotic cell cycle to repopulate a tumor. The underlying mechanisms that drive this cell cycle reprogramming remain unknown. C-myc is an oncogenic transcription factor that is dysregulated in ~70% of human cancers. C-myc is responsible for the activation of several cell cycle drivers, including cyclins and CDKs, and represses cell cycle inhibitors, such as CDK inhibitors. High levels of myc activity allow cells to move through the cell cycle quickly with a rapid doubling time. Documented endocycles in polyploid cells rely on high c-myc activity; however, our data shows that endocycling cancer cells have repressed levels of c-myc protein and transcriptional activity. Transcriptomic data shows that myc target genes are expressed at very low levels compared to the mitotic parental cells, and complementary data shows low protein levels. While myc downregulation is expected in non-proliferative cells, the compensatory mechanisms mediating the G1/S transition in the cancer endocycle remain unknown. Recent data has shown that myc suppression alone is not sufficient to induce a sustained endocycle at a population level, despite premature APCCdh1 activation during G2 in a subset of cells. We hypothesize that restoring myc activity to the levels of mitotic parental cells will drive endocycling cells to resume a proliferative mitotic cell cycle and produce mitotic progeny cells. Understanding the mechanisms that govern this transition is crucial to generate next-generation cancer therapeutics to disrupt endocycling cells, blocking acquired resistance and disease recurrence. Citation Format: Michael A. Loycano, Kenneth J. Pienta, Sarah R. Amend. Endocycling cancer cells undergo multiple S phases while repressing myc activity [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 515.

Abstract 5624: Dec2, a circadian rhythm gene facilitates pancreatic cancer dormancy through evasion of antitumor immunity

Abstract Metastatic recurrence following curative intent oncologic surgery is a major clinical problem in patients with pancreatic ductal adenocarcinoma (PDAC). These recurrences are from disseminated tumor cells (DTCs) and can sometimes occur years after surgery indicating that DTCs undergo a period of dormancy. The mechanisms that drive cancer dormancy and its awakening are poorly understood in part due to a lack of animal models that recapitulate dormant tumor cell biology. We created a novel model of PDAC dormancy where mice undergo surgery to remove the primary tumor and are followed for evidence of metastatic recurrence. Dormant DTCs can be isolated from mice without clinical evidence of recurrence for molecular studies. Single cell RNAseq of dormant DTCs detected that the circadian rhythm gene, Dec2 is upregulated. We knocked out (KO) Dec2 from murine pancreatic cancer cells and when implanted into the model we found almost no mice developed metastatic recurrence. When immunodeficient or immunocompetent mice depleted of CD4 and CD8 T cells were used as hosts, we observed no difference in survival in mice engrafted with Dec WT vs. KO cells indicating the survival benefit in the Dec KO group was due to an immune mediated mechanism. We performed a differential expression analysis on bulk RNAseq data comparing the Dec2 WT vs. KO cells and found antigen presentation a top upregulated pathway. We then observed that surface MHC-I was significantly upregulated in Dec2 KO cells as compared to wild type cells. Further mechanistic studies revealed that proteosome activity was significantly higher in Dec2 KO cells and treatment with proteosome inhibitors decreased surface MHCI levels in Dec2 KO cells. Cut and tag experiments revealed that Dec2 binds the promoter of a number of genes that regulate the proteosome. In conclusion, Dec2 promotes cancer dormancy by down regulating cell surface MHCI which facilitates immune evasion. Citation Format: Lan Wang, Chris Harris, Orjola Prela, Wade C. Narrow, Darren R. Carpizo. Dec2, a circadian rhythm gene facilitates pancreatic cancer dormancy through evasion of antitumor immunity [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 5624.

Abstract 4207: Zoledronic acid reduces quiescent bone-disseminated human ER+ breast cancer tumor cell burden and estrogen-responsiveness in a pre-clinical model

Abstract Unique to early-stage ER+ breast cancer, ER+ tumor cells already bone disseminated at time of diagnosis can remain dormant at this site until an initiating event (e.g., activating ESR1 mutation acquisition) allows for osteolytic bone metastases (BMET) progression even decades later. Adjuvant use of anti-resorptive bisphosphonates, but not denosumab, reduces BMET risk in post-menopausal women with early stage breast cancer, suggesting protective effects may be unrelated to changes in bone turnover. Pre-clinical modeling of dormant, bone-disseminated ER+ breast cancer cells may enhance our understanding and thus our ability to better target these inherently treatment-resistant cells. In studies reported here, a model of bone-disseminated ER+ dormancy was characterized and used to query effects of zoledronic acid (ZA) on dormant cells. When female nude mice were inoculated (intracardiac) with human ER+ MCF-7 cells labelled with DiD, a membrane dye that dissipates with each cell division, DiD-positive tumor cell number in harvested tibial bone marrow remained unchanged from 24 hours to 28 days post-tumor cell inoculation. When 17ß-estradiol (E2) treatment (0.72 mg 60-day pellets) was initiated on D28 following tumor cell inoculation, detectable DiD+ tumor cell number in harvested tibial bone marrow increased 1.8-fold within one week (D35 vs. D28, p < 0.0001), consistent with initiation of E2-stimulated proliferation, and osteolytic, histology-confirmed BMET appeared subsequently with 60% incidence by 6-weeks post-start of E2 treatment. When mice were instead zoledronic acid-treated during the 28d dormancy period (70 ug/kg subcutaneously on D7 and on D21 post-inoculation), DiD-positive cell number in marrow harvested on D28 was reduced by more than half vs. vehicle-treated controls (p< 0.05). Moreover, DiD-positive cell numbers on D35, one week after start of E2-treatment, remained unchanged in tibial marrow of ZA-treated mice and were thus substantially (3.8-fold) lower than controls (p< 0.001). When followed for 6-weeks after initiation of E2 treatment, no osteolytic BMET were detected in ZA-treated mice. In toto, these results recapitulate clinical findings, thus validating the methodologic approach, and suggest that in addition to reducing dormant ER+ tumor cell burden in bone, bisphosphonates may also alter their estrogen responsiveness. Acknowledgements: Special thanks to undergraduates Alyssa Magee and Geethika Ameneni for assistance with data acquisition, and the METAvivor Foundation and NIH-NCI (NCI R03 CA181893, R01 CA174926, P30 CA023074, T32 CA009213) for financial support. Citation Format: Julia N. Cheng, Jennifer B. Frye, Susan A. Whitman, Janet L. Funk. Zoledronic acid reduces quiescent bone-disseminated human ER+ breast cancer tumor cell burden and estrogen-responsiveness in a pre-clinical model [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 4207.

Genome-wide analysis of LOG family genes in castor and RcLOG5 enhances drought, salt, and cold stress tolerance in Arabidopsis thaliana

The gene encoding the specific phosphohydrolase LONELY GUY (LOG) plays an important role in the activation of cytokinin and the stress response in plant cells. However, the role of LOG genes in castor bean (Ricinus communis) has not been reported. In this study, we identified a total of nine members of the LOG gene family in the castor bean genome and investigated the upregulated expression of the RcLOG5 gene using transcriptome data analysis. We found that the RcLOG5 gene exhibited tissue-specific expression and was activated by polyethylene glycol, NaCl, low temperature, and abscisic acid stress. The subcellular localization results showed that the RcLOG5 gene is mainly located in the cytoplasm. Based on phenotypic and physiological indicators, namely root length, peroxidase activity, and malondialdehyde content, overexpression of the RcLOG5 gene not only improved the drought resistance, salt tolerance, and cold tolerance of transgenic Arabidopsis, but also shortened the dormancy period of the transgenic plants. Transcriptomic sequencing revealed that the overexpression of the RcLOG5 gene led to the enrichment of differentially expressed genes in the glutathione metabolism pathway in transgenic Arabidopsis. Moreover, the overexpression plants had higher levels of glutathione and a higher GSH/GSSG ratio under stress compared to the wild type. Therefore, we inferred that the RcLOG5 gene may be responsible for regulating cell membrane homeostasis by reducing the accumulation of reactive oxygen species through the glutathione pathway. Overall, the overexpression of the RcLOG5 gene positively regulated the stress resistance of transgenic Arabidopsis. This study provides valuable gene resources for breeding stress-tolerant castor bean varieties.

Dormancy of Macadamia Nut Seeds (Macadamia Integrifolia) by Drying and Soaking Water

Macadamia nut seeds are orthodox seeds that result in a dormancy period, so breaking the seed dormancy period is necessary. This study aims to determine the effect of drying and water immersion on dormancy breaking and macadamia bean seed germination and which drying and water immersion had the best impact on dormancy breaking and macadamia bean seed germination. The research was conducted in the soil laboratory and screen house of the Faculty of Agriculture, Winaya Mukti University, Tanjungsari District, Sumedang Regency, from June to November 2022. The experimental design used was a simple randomized block design (RBD) with a combination of drying and soaking seeds with nine treatment combinations. Each was repeated three times, so there were 27 experimental units. A = P 40oC + PA 45oC, B = P 45oC + PA 50oC, C = P 50oC + PA 55oC, D = P 50oC + PA 45oC, E = P 40oC + PA 50oC, F = P 45oC + PA 55oC, G = P 45oC + PA 45oC, H = P 40oC + PA 55oC, I = P 50oC + PA 50oC. The results showed that drying and soaking in water treatments at different temperatures had no significant effect on seed moisture content, seed cracking percentage, germination percentage, radicle length, and plumula length.

Dormancy Resistance of Macadamia Bean Seeds (Macadamia Integrifolia) by Drying and Water Immersion

Macadamia nut seeds are orthodox seeds that result in a dormancy period, so breaking the seed dormancy period is necessary. This study aims to determine the effect of drying and water immersion on dormancy breaking and macadamia bean seed germination and to determine which drying and water immersion had the best effect on dormancy breaking and macadamia bean seed germination. The research was carried out in the soil laboratory and screen house of the Faculty of Agriculture, Winaya Mukti University, Tanjungsari District, Sumedang Regency, from June to November 2022. The experimental design used was a simple randomized block design (RBD) with a combination of drying and soaking seeds with nine treatment combinations. Each was repeated three times, so there were 27 experimental units. A = P 40oC + PA 45oC, B = P 45oC + PA 50oC, C = P 50oC + PA 55oC, D = P 50oC + PA 45oC, E = P 40oC + PA 50oC, F = P 45oC + PA 55oC, G = P 45oC + PA 45oC, H = P 40oC + PA 55oC, I = P 50oC + PA 50oC. The results showed that drying and soaking in water treatments at different temperatures had no significant effect on seed moisture content, seed cracking percentage, germination percentage, radicle length, and plumula length.

Integrated proteomic, transcriptomic, and metabolomic profiling reveals that the gibberellin-abscisic acid hub runs flower development in the Chinese orchid Cymbidium sinense.

The seasonal flowering Chinese Cymbidium produce an axillary floral meristem and require a dormancy period during cold conditions for flower development. However, the bud activation mechanism remains elusive. This study evaluates the multi-omics across six stages of flower development, along with functional analysis of core genes to decipher the innate mechanism of floral bud initiation and outgrowth in the Chinese orchid Cymbidium sinense. Transcriptome and proteome analyses identified 10 modules with essential roles in floral bud dormancy and activation. Gene clusters in the early stages of flower development were mainly related to flowering time regulation and meristem determination, while the late stages were correlated with hormone signaling pathways. The metabolome identified 69 potential hormones in which gibberellin (GA) and abscisic acid (ABA) were the main regulatory hubs, and GA4 and GA53 exhibited a reciprocal loop. Extraneous GA application caused rapid elongation of flower buds and promoted the expression of flower development genes. Contrarily, exogenous ABA application extended the dormancy process and ABA inhibitors induced dormancy release. Moreover, CsAPETALA1 (CsAP1) was identified as the potential target of ABA for floral bud activation. Transformation of CsAP1 in Arabidopsis and its transient overexpression in C. sinense protoplasts not only affected flowering time and floral organ morphogenesis in Arabidopsis but also orchestrated the expression of flowering and hormone regulatory genes. The presence of ABA response elements in the CsAP1 promoter, rapid downregulation of CsAP1 after exogenous ABA application, and the activation of the floral bud after ABA inhibitor treatment suggest that ABA can control bud outgrowth through CsAP1.

Understanding the influence of ultrasonic power on the hydration of cement paste

This paper studied the dispersion and hydration of cement paste prepared by power ultrasound (PUS)-assisted mixing technology. The ultrasonic power used were 0 W, 480 W and 912 W. The hydration process and microstructure were tested by using isothermal calorimetry, X-ray diffraction (XRD), thermogravimetric-differential scanning calorimetry (TG-DSC), solid-state nuclear magnetic resonance silicon (29Si-NMR) spectroscopy, mercury intrusion porosimetry (MIP), attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, scanning electron microscopy (SEM) and energy dispersive spectroscopy (SEM‒EDS). The results indicated that the dynamic hydration kinetics of cement paste under ultrasonic irradiation can be categorized into crystallization nucleation and growth (NG), phase boundary (I) and diffusion (D) processes. The apparent rate constant KNG in the NG process and KI in the I process both increased as the ultrasonic power increased, suggesting that PUS-assisted mixing accelerated the early hydration process. PUS shortened the dormant period and enhanced the total hydration heat release. The capillary pores and gel pores were both reduced at 28 days. Similarly, compared with those of C0 and C480, the gel porosities of C912 decreased by 35.17% and 31.87%, respectively. In addition, the mean chain length of the C–S–H gel increased with increasing ultrasonic power, which effectively filled the nanoscale defects. PUS improved the early strength and increased the strength at late hydration ages. Compared with that in the reference group, the compressive strength in the C912 group was enhanced by 26.1% at 1 day and 18.3% at 28 days. These findings demonstrate that power ultrasound-assisted mixing is advantageous for enhancing the sustainability of building materials, thereby contributing to the development of future circular buildings.

On thermodynamics and heat and mass transfer aspects of CH4 adsorption onto coconut shell-based carbonaceous material

The current study investigates the equilibrium methane (CH4) adsorption capacity and the adsorption kinetics of a locally produced coconut shell-derived activated carbon (AC CARB 6X12 60) for pressure and temperatures ranging from 0 to 55 bar and -20 to 70 °C, respectively. The adsorption isotherms and kinetics are determined using an in-house developed adsorption test rig. A maximum methane adsorption uptake of 0.140 kg/kg is obtained at -20 °C and 40 bar pressure. The adsorption equilibrium isotherm data are fitted using Toth, Dubinin-Astakhov (DA), and Modified-DA isotherm models. Afterwards, the kinetics study is done considering pseudo first order, second order, Elovich, and intra-particle diffusion models. In continuation, based on the DA isotherm model, Clausius Clapeyron relation, and Maxwell's relations, the expressions for the crucial thermodynamic parameter of adsorbed phase, i.e., isosteric heat of adsorption, specific heat capacity, enthalpy, and entropy is derived, and same is estimated using DA isotherm parameters. Additionally, the activated carbon's thermophysical properties (i.e., thermal conductivity, thermal diffusivity, and specific heat) are determined using the TPS 2500 S analyzer, which works on the hot-disk principle. Using the most suitable isotherm (DA) and kinetic model (pseudo second order), heat and mass transfer analysis is carried out on novel reactor configuration with an external cooling jacket, double row of cooling pipes with external longitudinal fins for methane storage using a 3D transient model in Comsol Multiphysics. The charge characteristics are studied for gas charging pressure varying from 1 to 50 bar and cooling fluid temperature of -20 to 70 °C. Iso-concentration contours are plotted to identify the pressure-temperature combination resulting in identical storage capacity (i.e., including both gaseous and adsorbed phases). Further simulations are carried out for constant pressure charging and constant flow discharge conditions to expound upon the effects of the dormancy period, gas charging pressure, and cooling/heating fluid temperature. 14% improvement in total discharge amount is obtained with a dormancy period between charging and discharging compared to no dormancy case.

The Effect of Benzyl Amino Purin and NaphtalenaAcetic Acid Applications on Direct Shoot Organogenesis in Porang (Amorphophallus muelleri B)

Porang (Amorphophallus muelleri B.) is a tuberous plant with the potential to be the main source of carbohydrates and is rich in benefits. Porang proliferation is limited by a 6-month dormancy period per year, and generative propagation is unlikely due to the seeds being apomictic triploid. The aimed of my research to analyze the application of BAP and NAA in culture media for direct propagation of porang shoots. The explant used in this research was young leaves. The research was arranged in a completely randomized design with a combination of BAP and NAA hormones added to the MS medium. There were three BAP treatments, namely 1.0 mg/L, 2.0 mg/L, and 3.0 mg/L, while NAA treatments consisted of 2 levels, namely 2.0 mg/L and 4.0 mg/L. The addition of 1.0 mg/L BAP combined with 4.0 mg/L NAA was the best treatment that produced seven shoots with an average shoot length of 2.14 cm and root length of 3.6 cm, with the earliest bud emergence (9.7 weeks after planting).

Breaking Dormancy of Shallot (Allium ascolonicum L.) Bulb Using Hydrogen Peroxide

Shallot bulbs have a dormancy period, so they must be stored for 3-4 months. This condition inhibits the acceleration of planting, which has a short time, so efforts are needed to accelerate the dormancy period by using hydrogen peroxide. This research was carried out from June to July 2023 at the Screen House, Teaching Farm, Faculty of Agriculture, Hasanuddin University. The study was arranged in a randomized block design with a two-factor factorial design. The first factor is the variety of Mentes, Rubaru, Violetta 2 Agrihorti, Kramat 1, and Ambassador 3 Agrihorti. The second factor is hydrogen peroxide concentration with three levels, namely 0%, 10% and 20%. The results showed that breaking the dormancy of shallot bulbs using hydrogen peroxide at a concentration of 20% effectively triggered the process of bulb growth in the various shallot varieties tested, marked by an increase in the percentage of rooted and sprouted bulbs. The Rubaru and Mentes varieties had an excellent response to 20% hydrogen peroxide immersion, indicated by a shorter rooting and sprouting time, compared to other varieties and concentrations of hydrogen peroxide. Therefore, hydrogen peroxide with a concentration of 20% can be used to break dormancy in shallot bulbs. However, further studies are needed on growth and production in the field and the quality and safety of shallot bulbs. Keywords: Bulb, Dormancy, Hydrogen peroxide, Shallot.

Transcriptome Analysis Reveals the Molecular Mechanisms of BR Negative Regulatory Factor StBIN2 Maintaining Tuber Dormancy.

Potato is an important food crop. After harvest, these tubers will undergo a period of dormancy. Brassinosteroids (BRs) are a new class of plant hormones that regulate plant growth and seed germination. In this study, 500 nM of BR was able to break the dormancy of tubers. Additionally, exogenous BR also upregulated BR signal transduction genes, except for StBIN2. StBIN2 is a negative regulator of BR, but its specific role in tuber dormancy remains unclear. Transgenic methods were used to regulate the expression level of StBIN2 in tubers. It was demonstrated that the overexpression of StBIN2 significantly prolonged tuber dormancy while silencing StBIN2 led to premature sprouting. To further investigate the effect of StBIN2 on tuber dormancy, RNA-Seq was used to analyze the differentially expressed genes in OE-StBIN2, RNAi-StBIN2, and WT tubers. The results showed that StBIN2 upregulated the expression of ABA signal transduction genes but inhibited the expression of lignin synthesis key genes. Meanwhile, it was also found that StBIN2 physically interacted with StSnRK2.2 and StCCJ9. These results indicate that StBIN2 maintains tuber dormancy by mediating ABA signal transduction and lignin synthesis. The findings of this study will help us better understand the molecular mechanisms underlying potato tuber dormancy and provide theoretical support for the development of new varieties using related genes.

Classification of cultivars of common peach (Persica vulgaris Mill.)

The existing classifications of peach cultivars are based on the morphological characteristics of vegetative and generative organs, and are of economic importance. The classification by N. I. Ryabov (1939) gained the greatest popularity. The entire cultural assortment of Persica vulgaris Mill. was divided by him into two varieties distinguished by the type of flower. A variety with a rose-shaped flower, P. vulgaris var. rosaeflora Rjab., is divided into 4 groups: 1. American early ripers; 2. Northern Chinese peaches of the ‘Chinese Cling’ type (with cartilage-like flesh texture); 3. cultivars of the Turkestan-Transcaucasian type; 4. South Chinese ‘Honey’ type peaches (honey peaches). A variety with a bell-shaped flower, P. vulgaris var. campanuliflora Rjab., is divided into two groups: 1. Iranian, the ‘Phillips Cling’ type (with cartilage-like flesh texture); 2. Iranian, the ‘Crawford’ type (with a fibrous flesh texture). The work was carried out in the collection orchard of the Research Institute of Plant Genetic Resources of Uzbekistan.Research has shown that the groups do not have a specific geographical area and consist of cultivars from all around the world. Peach cultivars are grouped according to a set of morphological characteristics, therefore, the average values of basic biological properties within a group are very close and the differences are insignificant, which complicates the use of the classification for scientific purposes.The proposed classification of peach cultivars is based on the botanical-geographical principle (Vavilov, 1935). It is proposed to divide all common peach cultivars into groups according to their geographical origin: 1. Chinese; 2. Transcaucasian; 3. European; 4. American; and 5. Central Asian. Each of these groups has its own distribution area. The biological properties of cultivars were formed under the influence of various soil and climatic conditions. Chinese peaches are frost and winter hardy and have a long dormancy period. However, in terms of commercial and taste qualities they are inferior to modern cultivars. They can be used in breeding for creating large-fruited, frostresistant, late flowering varieties. European cultivars were forming under different conditions and they should be used differentially; they are good source material for breeding cultivars with high commercial and taste qualities of fruits. Transcaucasian varieties are superior to other groups in terms of transportability. These are cultivars for canning and high quality processed products. They are relatively resistant to fungous diseases. American cultivars are distinguished by high commercial and taste qualities of fruits. Most cultivars are highly frost-resistant, but they are poorly winterhardy. They can be used for breeding highly productive cultivars with high commercial and taste qualities of fruits, with different ripening periods. Central Asian varieties have a long dormancy period, they are frost-, drought-, and soil salinity resistant. However, they are weakly resistant to fungous diseases. They can be used in breeding according to the indicated characteristics

Genome-wide characterization of SDR gene family and its potential role in seed dormancy of Brassica napusL.

Rapeseed (Brassica napus L.) with short or no dormancy period are easy to germinate before harvest (pre-harvest sprouting, PHS). PHS has seriously decreased seed weight and oil content in B. napus. Short-chain dehydrogenase/ reductase (SDR) genes have been found to related to seed dormancy by promoting ABA biosynthesis in rice and Arabidopsis. In order to clarify whether SDR genes are the key factor of seed dormancy in B. napus, homology sequence blast, protein physicochemical properties, conserved motif, gene structure, cis-acting element, gene expression and variation analysis were conducted in present study. Results shown that 142 BnaSDR genes, unevenly distributed on 19 chromosomes, have been identified in B. napus genome. Among them, four BnaSDR gene clusters present in chromosome A04、A05、C03、C04 were also identified. These 142 BnaSDR genes were divided into four subfamilies on phylogenetic tree. Members of the same subgroup have similar protein characters, conserved motifs, gene structure, cis-acting elements and tissue expression profiles. Specially, the expression levels of genes in subgroup A, B and C were gradually decreased, but increased in subgroup D with the development of seeds. Among seven higher expressed genes in group D, six BnaSDR genes were significantly higher expressed in weak dormancy line than that in nondormancy line. And the significant effects of BnaC01T0313900ZS and BnaC03T0300500ZS variation on seed dormancy were also demonstrated in present study. These findings provide a key information for investigating the function of BnaSDRs on seed dormancy in B. napus.

Comparative transcriptomic evidence of physiological changes and potential relationships in vertebrates under different dormancy states.

Dormancy represents a fascinating adaptive strategy for organisms to survive in unforgiving environments. After a period of dormancy, organisms often exhibit exceptional resilience. This period is typically divided into hibernation and aestivation based on seasonal patterns. However, the mechanisms by which organisms adapt to their environments during dormancy, as well as the potential relationships between different states of dormancy, deserve further exploration. Here, we selected Perccottus glenii and Protopterus annectens as the primary subjects to study hibernation and aestivation, respectively. Based on histological and transcriptomic analysis of multiple organs, we discovered that dormancy involved a coordinated functional response across organs. Enrichment analyses revealed noteworthy disparities between the two dormant species in their responses to extreme temperatures. Notably, similarities in gene expression patterns pertaining to energy metabolism, neural activity, and biosynthesis were noted during hibernation, suggesting a potential correlation between hibernation and aestivation. To further explore the relationship between these two phenomena, we analyzed other dormancy-capable species using data from publicly available databases. This comparative analysis revealed that most orthologous genes involved in metabolism, cell proliferation, and neural function exhibited consistent expression patterns during dormancy, indicating that the observed similarity between hibernation and aestivation may be attributable to convergent evolution. In conclusion, this study enhances our comprehension of the dormancy phenomenon and offers new insights into the molecular mechanisms underpinning vertebrate dormancy.

Hydration of Early‐Age Composite Cement Paste Using Low‐Field Nuclear Magnetic Resonance

Low‐field nuclear magnetic resonance is adopted in this paper for the study of the hydration process of early‐age pure and composite cement paste by monitoring the longitudinal relaxation time (T1) of water constrained in the paste. Based on the correlation between the changes of weighted average T1 and the hydration process of paste, the hydration process can be divided into four stages, i.e., initial period, dormant period, accelerated period, and steady period. The effect of water‐to‐cement ratio (w/c) and admixtures to hydration is discussed in detail. Compared to WC04, the weighted average T1 at the end of the dormant period increased by 4.79, 1.74, and 0.05 ms when 30% fly ash, 30% slag, and 10% silica fume were added, respectively. Based on experimental data and the Avrami–Erofeev equation, a hydration model for both pure and composite cement pastes is proposed, with a correlation that is higher than 0.96, which can describe the hydration process of paste well.

Regulation of Seed Dormancy Genes in Triticeae Species.

Wild progenitors of Triticeae crops generally have long dormancy periods. Domesticated crops inherited these longer dormancy alleles from their wild progenitors, which have since been modified and selected during cultivation and utilization by humans. Thus, allelic combinations at different seed dormancy loci are currently represented in Triticeae germplasm preserved in seed repositories and gene banks as accessions and materials of breeding programs. Methods to evaluate seed dormancy are key to explore, analyze, and exploit optimal alleles in dormancy genes. Recent developments in genomics have accelerated the identification and analysis of seed dormancy loci in Triticeae species. Transgenic experiments have been conducted to validate if candidate genes affect seed dormancy and more recently have yielded an array of mutations derived from genome editing for practical applications. The information gathered on these seed dormancy loci provides a deeper knowledge of germplasm diversity and offers strategies to control seed dormancy in breeding programs in Triticeae crops.