Periods Of Deficit Research Articles

THE INFLUENCE OF COMPLEX IMPLEMENTATION OF THE ERAS PROGRAM ON THE SURGICAL STRESS RESPONSE FACTORS IN PEDIATRIC PATIENTS UNDERGOING ELECTIVE LIMB SURGERY

Surgical stress response, fluid and nutritional balance, and pain management are among the key factors influencing on the postoperative period. These areas are reflected in the Enhanced Recovery After Surgery (ERAS) concept, which is based on a set of measures aimed at minimizing the surgical stress response. The lack of ERAS recommendations for the pediatric population of surgical patients prompts to search for a solution of adaptation and implementation of the ERAS programs in children. The aim of the study. To determine the effectiveness of the complex application of the enhanced recovery after surgery by studying its influence on the factors of the surgical stress response in pediatric patients undergoing limp surgery. Materials and methods. The prospective randomized study included 47 patients aged 1 to 17 years. In the study group (n = 22), the ERAS program was used, which consisted of 14 components of preoperative, intraoperative and postoperative measures. In the control group (n = 25), the complex of all ERAS components was not purposefully applied. We compared glycaemic stress index (GSI) and length of hospital stay after surgery (LOS) as a primary outcome; secondary outcomes were fasting time after drinking fluids before induction of anesthesia, estimated fluid deficiency just before induction of anesthesia, hemodynamic parameters, the need for opioids during and after surgery, blood glucose and beta-hydroxybutyrate in the beginning and in the end of the surgery and next day morning after surgery, postoperative nausea and vomiting. Results. GSI was significantly less in the study group (1,62 ± 0,78 vs 2,12 ± 0,93, р=0,046). LOS in the study group was 37.5% less than in the control group (p = 0.002). The period of preoperative fasting and fluid deficit in the patients of the study group were shorter. The needs of fentanyl intraoperatively in the study group was 47% less (p<0.001). At the beginning of surgery, the study group had a higher blood glucose concentration and a higher systolic blood pressure. At the end of surgery, blood glucose and beta-hydroxybutyrate in the study group was lower than in the control group. In the postoperative period, 9% of patients in the study group and 36% of patients in the control group complained of nausea (p = 0.041). Linear regression analysis showed a linear relationship between the LOS after surgery and the GSI (R=0,515, R2=0,266, B=2,156, 95% CI 1,04 – 3,27, p<0,001). Results. The implementation of the ERAS program to children 1-17 years old undergoing elective limp surgery may reduce the effect of the surgical stress reaction by reducing the period of preoperative fasting, fluid deficiency, the needs of opioids and postoperative nausea. Minimizing the surgical stress response may facilitate the achievement of hospital discharge criteria and shorten the length of hospital stay in the postoperative period.

The Effect of Water Stress on Total-Phenolic Content of Barley

Little is known about the relation between water stress and the accumulation of phenolics in plant tissues. The present study aimed to investigate the effect of water stress and maturation on the production of total-phenolics (TP) by four barley (Hordeum vulgare L.) varieties (‘Manel’, ‘Martin’, ‘Rihane’, ‘Espérance’). During three phenological stages (S-8, S-10.5, S-11), following Feekes scale, whole barley plants were pulled out of the field and separated into roots, stems, and leaves. Water extracts were prepared from plant parts and their TP contents were determined by spectrophotometer. To determine periods of water deficit (WD) at field, climatic characterization of the region was carried out. TP accumulated in barley plant and its parts under the influence of water deficit essentially at S8, which coincided with barley spring growth. However, TP content decreased when WD became more pronounced at the following stages. This response may be explained, partially by the biosynthesis of lignin from free phenols when the plant approached maturity. Results suggest that water stress stimulates the synthesis and accumulation of TP in barley tissues during active growth periods (spring growth) at S-8. This response doesn’t persist until the critical periods of WD where barley maturity favors a decrease in TP content for all plant parts. Regardless of growth stage and WD, barley accumulates preferentially phenolics in above-ground plant parts. The evolution of phenolic accumulation under water stress showed the same trends for the tested barley varieties, indicating a genetic control of phenolic production and their partitioning across plant parts.

Water use of selected cover crop species commonly grown in South African fruit orchards and their response to drought stress

Cover crops are widely planted in orchards for a variety of reasons. These include suppressing soil erosion, nutrient cycling, phytosanitary purposes, general orchard aesthetics etc. However, there is need to balance these benefits against use of scarce resources such as water and nutrients. Currently no information exists on how different cover crop species use water in orchards and how they cope with drought stress. The aim of this study was therefore to compare the transpiration dynamics of various cover crop types in order to identify species with conservative water use rates. Studied species included: 1) two exotic legumes i.e. Lupine (Lupinus albus L.), and Common vetch (Vicia sativa), 2) three exotic grasses i.e. Tall fescue (Festuca arundinacea), Rye grass (Loliumperenne), and Kikuyu grass (Pennisetum clandestium) and; 3) grasses that are indigenous to sub-Saharan Africa i.e. African Lovegrass (Eragrostis capensis) and Rhodes grass (Chloris gayana). The crops were planted in pots under controlled greenhouse conditions. Transpiration rates were quantified using miniature stem heat balance sap flow gauges and by manual weighing. Drought stress was imposed by withholding irrigation at selected intervals and the responses were quantified through changes in the water relations of the plants. The study showed that exotic legumes had the highest daily water use which peaked at about 2.4 L per square metre of leaf area per day, followed by exotic grasses at 1.5–2.0 L/m2/d. The indigenous grasses used the least water ranging from 0.8 to 1.2 L/m2/d. The indigenous grasses largely displayed an isohydric response to drought stress by maintaining their leaf water status with increasing soil water deficit. The exotic species, on the other hand, showed risk taking behaviour (anisohydry) wherein both the transpiration and leaf water status decreased sharply as drought stress increased. Consequently, some exotic species failed to recover when stress was relieved. From a water use perspective, this study demonstrates that indigenous grass species are more appropriate as cover crops in South African orchards because of their low transpiration rates and the ability to cope with extended periods of water deficit.

The Dry and the Wet Case: Tree Growth Response in Climatologically Contrasting Years on the Island of Corsica

Stem radial variations of Corsican Black pine (Pinus nigra Arnold subsp. laricio Maire) and Maritime pine (Pinus pinaster Aiton) were monitored to quantify the impact of two meteorologically contrasting consecutive years. On the French island of Corsica, in the western Mediterranean basin, the year 2017 was extremely dry, while 2018 was exceptionally wet. We attached electric band dendrometers to 36 pines along an east–west transect, spanning the central mountain range, and set up automated weather stations at all five sites, ranging from 10 m asl to 1600 m asl. Stem radial variations (SRV) were separated into irreversible growth (GRO) and tree water deficit (TWD) periods. During the drought of 2017, the most severe tree water deficits occurred in the western part of the island, whereas trees at higher elevations were more affected than at lower elevations. A prolonged decrease of SRV, even close to the tree line, suggests bimodal growth and reveals high plasticity of growth patterns in both Corsican pines. Stem radial variations correlated significantly with precipitation and temperature. The positive correlations of GRO with precipitation and the negative correlations of TWD with temperature imply that high evapotranspiration led to the intense period of TWD in 2017. A novel approach was used to further investigate the growth/climate relationship by including synoptic-scale pressure situations. This revealed that an elevation gradient in GRO per weather pattern was only present in the wet year and that even rarely occurring weather patterns can have a substantial impact on tree growth. This novel approach provides a more comprehensive insight into meteorological drivers of tree growth patterns by incorporating different scales of the climatic system.

Functional Diversity and Assembly Rules of Two Deciduous Seasonal Forests in Southeastern Brazil

Abstract Deciduous seasonal forests (DSFs) have a peculiar floristic composition, with species capable of surviving periods of high water deficit in a year. Our goal was to demonstrate that abiotic filters lead to the assembly of two DSF communities that have high floristic dissimilarity. For this, we characterized the environment of the areas and used the community-weighted mean (CWM), functional richness (FRic) and functional dispersion (FDis) indices for regional recognition of functional patterns. The local assessment of assembly rules was carried out using null models. We found differences in the FRic and FDis between the areas, which was attributed to the different floristic influences exercised on the communities. However, in both, the typical attributes of dry formations were dominant (CWM), indicating that, on the regional scale of study, the dry season acts as a filter in the composition of species in the communities. On a local scale, stochastic dispersion was identified as the most influential mechanism in the assembly of communities. We conclude that deterministic and stochastic processes act in the assembly of the studied communities, and the proportion of each of these depends on the scale, with abiotic filtration predominating on a regional scale and stochastic dispersion events on a local scale.

Characterization and Quantification of Meteorological Drought in the Oued El-Abid Watershed, Central High Atlas, Morocco (1980-2019)

The present study is focused on analysis of rainfall in the Oued El-Abid watershed, which is characterized by an important potential in water supply of the Bin El Ouidane dam and the recharging groundwater of the plains downstream. The aim of the present research is to characterize the meteorological drought in the Oued El-Abid watershed, located in the Beni Mellal-Khenifra region (Central High Atlas, Morocco). The study focused on the analysis of the meteorological drought detection indices such as the deviation from the mean (DM), the rainfall index (RI) and the standardized precipitation index (SPI) based on annual precipitation for the three stations (Tilouguit, Ait Ouchen and Tizi N'Isli) generally experienced alternating periods of surplus and deficit. The results of these indices allowed us to determine the most remarkable and common drought years are: 1981, 1983, 1990, 1998, 2001, 2005, 2017 and 2019. This study is helpful for water resource managers to make decisions and develop tools for adaptation and mitigation of climate change impacts.

The residual mass severity index – A new method to characterize sustained hydroclimatic extremes

Water supply management for hydroclimatic extremes has commonly been analysed using the cumulative difference between inflow and demand, a concept known as the Residual Mass Curve. This paper extends the residual mass concept to develop a new method to quantify water availability, termed the Residual Mass Severity Index (RMSI). The RMSI improves on the Residual Mass Curve by allowing extreme deficit periods to be easily identified without the need for manual inspection of the Residual Mass Curve. Another advantage of the RMSI is that it does not require a temporal window to be specified to characterise extreme deficit periods, which is needed for many drought indices such as the Standardized Precipitation Index (SPI). This paper develops the method for calculating the RMSI using precipitation time series and compares it to the SPI for different drought durations across Australia. Major hydroclimatic extremes during the instrumental period are assessed using RMSI, SPI, and by comparing the RMSI estimates with streamflow data from 467 catchments with varying sizes and climate across Australia. RMSI is a useful index for water managers interested in assessing water availability with potential applicability in summarising the features of historical hydroclimatic extremes as well as future changes in water availability due to anthropogenic climate change.

Drought and Competition With Ivyleaf Morningglory (Ipomoea hederacea) Inhibit Corn and Soybean Growth

Agricultural impacts of climate change include direct effects on crop plants and indirect effects, such as changes to the distributions and competitiveness of weed species. In the northeastern United States, warming temperatures are likely to result in periods of soil moisture deficit and changes to weed communities. Ivyleaf morningglory (IMG, Ipomoea hederacea Jacq.) is a summer annual vine that competes with field crops and interferes with harvesting. Climate change may increase the competitive effects of IMG on northeastern U.S. field crops. We conducted a greenhouse study to evaluate the effects of IMG on corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] under drought and non-drought conditions. The drought treatment was crossed against an IMG competition treatment with five levels: one crop plant without IMG plants, one crop plant with one, two, or three IMG plants, and one IMG plant without crop plants. Both drought and IMG (presence or biomass) reduced the biomass of corn and soybean (P < 0.05). Drought and IMG (presence) reduced soybean pod production (P < 0.001). IMG biomass was reduced by drought and the presence of corn (P < 0.001). Across all competition treatments, drought reduced IMG biomass by 71% in the corn experiment and 79% in the soybean experiment, compared with a corn biomass reduction of 50% and a soybean biomass reduction of 58%. Well-designed management programs should mitigate the risks associated with stressors such as IMG and drought, which may threaten northeastern U.S. field crop production under climate change.

CLIMATE VARIABILITY AND IMPACT VULNERABILITY STATUS OF IRRIGATION WATER RESOURCES ON RICE AND TOMATO PRODUCTION DOWNSTREAM OF TIGA DAM, NIGERIA

The focus of this empirical study is to investigate the trends of some hydro metrological parameters and Impact Vulnerability Status (IVS) of irrigation water resources on rice and tomato production in the downstream of Tiga station. Investigation was conducted using data on rainfall, temperature, evaporation and reservoir water level for 30 years in Tiga station. The data collected was used to show the trend fluctuations of each parameter for the period of study. The rainfall data was also used to analyze the Normalized Rainfall Index (NRI) in order to know periods of surplus, deficit and optimal water availability as against the required water for rice and tomato production. The rainfall pattern and water level showed increasing trend while temperature and evaporation showed a general decrease in trend. The NRI used to investigate the IVS in Tiga station downstream revealed that rice and tomato were not vulnerable to drought and flooding for 18 years while every other years were vulnerable or slightly vulnerable. However, only year 1993 appears to be very wet and highly susceptible to flooding. Findings from focus group revealed that 80% of the farmers reported floods occurrences during rainy season and deficit of water between January and March of each year. In conclusion, the IVS of farmers to climate change revealed periods of deficit, optimal and excess water availability for rice and tomato production and their vulnerability status. It was recommended that the government should strengthen laws and policies relevant in addressing climate change

Environmental flow sustainability in the Lower Limpopo River Basin, Mozambique

Study regionThis study focuses on the Lower Limpopo River basin (LLRB) in Mozambique, Africa. Study focusMaintaining environmental flows necessary for ecosystem sustainability represents a significant challenge to water resource management. In this study the sustainability of LLRB was evaluated by comparing hydrologic availability with ecological and anthropogenic needs. Current river ecological status was scored with a habitat integrity index verified through ground-truthing field surveys and aerial imagery data. Local stakeholder interviews were used to further evaluate the habitat index scores. Deficiencies between water availability and ecological-human requirements were assessed with a water scarcity index. New Hydrological Insights for the RegionFour environmental flow categories defined as “Excellent”, “Fair”, “Poor”, and “Degraded” coincided to approximately 50 %, 39 %, 27 %, and 14 % of the natural mean annual flow, respectively. Stakeholder interview responses indicated annual water shortages currently occur between August and November and coincide with “Poor” and “Degraded” environmental flow conditions. Water supplies appear to meet consumption needs when calculated on an annual basis with the water scarcity index. However, when calculated monthly, there is not enough to meet human water demand between August and October. This deficit period will likely expand from June to November due to projected increases in future water demands. As the greatest water use in the basin is agricultural irrigation, long-term environmental flows sustainability will likely depend upon effective irrigation management.

Aeration and Stirring in Yarrowia lipolytica Lipase Biosynthesis during Batch Cultures with Waste Fish Oil as a Carbon Source

Yarrowia lipolytica is one of the most studied non-conventional forms of yeast, exhibiting a high secretory capacity and producing many industrially important and valuable metabolites. The yeast conceals a great biotechnological potential to synthesize organic acids, sweeteners, microbial oil, or fragrances. The vast majority of bioprocesses are carried out in bioreactors, where suitable culture conditions are provided. In the current study, the effect of agitation speed (200–600 rpm) and air flow rate (0.0375–2.0 dm3/(dm3 × min)) on the biomass yield and lipase activity of Y. lipolytica KKP 379 is analyzed in a growth medium containing waste fish oil. The increase of aeration intensity limited the period of oxygen deficit in the medium. Simultaneously, an increase in lipolytic activity was observed from 2.09 U/cm3 to 14.21 U/cm3; however, an excessive agitation speed likely caused oxidative or shear stresses, and a reduction in lipolytic activity was observed. Moreover, it is confirmed that the synthesis of lipases is related to oxygen consumption, pH, and the yeast growth phase, and appropriate process selection may provide two advantages, namely, the maximum use of the waste carbon source and the production of lipolytic enzymes that are valuable in many industries.

PENGARUH PERUBAHAN IKLIM TERHADAP EKOFISIOLOGI TANAMAN KELAPA SAWIT (Elaeis guineensis Jacq.)

Distinction in oil palm growth and productivity are caused by the interaction of genotypes and the environment. Therefore, environmental changes, especially climate change, have a negative impact on oil palm growth and production. Climate change that affects oil palm yields in the tropics and sub-tropics consists of two important factors, namely water availability and temperature. The first impact of a period of water deficit is a decrease in gas exchange, during the dry season photosynthesis can be inhibited due to an increase in water pressure deficit which causes stomata to close. The dry season is associated with high temperatures, which causes a decrease in photochemical efficiency temperature, decreased photosynthesis and decreased transpiration. Water deficit affects oil palm male flower production. High temperatures increase photorespiration and dark respiration so that the total biomass production decreases. Oil palm breeding program must be a multidisciplinary effort that involves molecular tools, understanding the response of oil palm cultivation to limiting conditions caused by abiotic.

SOCIO-DEMOGRAPHIC AND MEDICO-BIOLOGICAL FACTORS AS PROGNOSTIC INDICATORS OF QUALITY OF LIFE IN EARLY CHILDHOOD

Patient’s quality of life (QL) measures are endowed with independent predictive value and these factors are considered to be more distinct than patient’s general somatic condition for predicting patient’s health condition. However, the number of researches devoted to QL prediction in the field of medical science is low. The aim of research is evaluation of predictive measure of QL of early aged children. Prospective observational study was carried out. The objects of the research were 2362 early age children (3months-3years old) from pediatric polyclinics of Yerevan. QL of children was evaluated with the international questionnaire “QUALIN”. Wald’s analytical method has been applied for predictive evaluation of QL criteria and formation of risk group. For the analysis and evaluation of the statistical material used SPSS Statistics software package. In social-hygienic factors more important were: family type, conflicts in family, disabled child and frequent morbidity families, presence of artificial nutrition since birthday. Among medico-biological factors the presence of two or more diseases in neonatal period, low and high levels of physical development, weight deficit and obesity, child’s health group and respiratory, nervous and digestive system diseases were more significant. In terms of predictive evaluation of QL, it can be stated that a number of medico-biological and socio-hygienic factors affect the overall formation of QL. By means of predictive evaluation of QL one can originally set apart targeted risk groups and if the score of predictive evaluation is +13 and higher, implement health measures, which may provide with improvements of QL criteria.

Scrutinizing the impact of water deficit in plants: Transcriptional regulation, signaling, photosynthetic efficacy, and management

Suboptimal availability of water limits plant growth, development, and performance. Drought is one of the leading factors responsible for worldwide crop yield reduction. In the future, owing to climate changes, more agricultural land will be affected by prolonged periods of water deficit. Thus, understanding the fundamental mechanism of drought response is a major scientific concern for improvement of crop production. To combat drought stress, plants deploy varied mechanistic strategies and alter their morphological, physiochemical, and molecular attributes. This helps plant to enhance water uptake and storage, reduce water loss and avoid wilting. Induction of several transcription factors and drought responsive genes leads to synthesis of stress proteins, regulation of water channels i.e. aquaporins and production of osmolytes that are essential for maintenance of osmotic balance at the cellular level. Self- and hormone-regulated signaling pathways are often stimulated by plants after receiving drought stress signals via secondary messengers, mitogen-activated protein kinases, and stress hormones. These signaling cascades often leads to stomatal closure and reduction in transpiration rates. Reduced carbon dioxide diffusion in chloroplast, lowered efficacy of photosystems, and other metabolic constraints limits the key regulatory photosynthetic process during water deficit. The impact of these stomatal and nonstomatal limitations varies with stress intensity, superimposed stresses and plant species. A clear understanding of the drought resistance process is thus important before adopting strategies for imparting drought tolerance in plants. These management practices at present include exogenous hormone application, breeding, and genetic engineering techniques for combating the water deficit issues.

Drought affects the rate and duration of organ growth but not inter-organ growth coordination.

Drought at flowering and grain filling greatly reduces maize (Zea mays) yield. Climate change is causing earlier and longer-lasting periods of drought, which affect the growth of multiple maize organs throughout development. To study how long periods of water deficit impact the dynamic nature of growth, and to determine how these relate to reproductive drought, we employed a high-throughput phenotyping platform featuring precise irrigation, imaging systems, and image-based biomass estimations. Prolonged drought resulted in a reduction of growth rate of individual organs-though an extension of growth duration partially compensated for this-culminating in lower biomass and delayed flowering. However, long periods of drought did not affect the highly organized succession of maximal growth rates of the distinct organs, i.e. leaves, stems, and ears. Two drought treatments negatively affected distinct seed yield components: Prolonged drought mainly reduced the number of spikelets, and drought during the reproductive period increased the anthesis-silking interval. The identification of these divergent biomass and yield components, which were affected by the shift in duration and intensity of drought, will facilitate trait-specific breeding toward future climate-resilient crops.

Ecophysiology of peanuts grown under water deficit regimes

This study evaluates physiological aspects of two peanut cultivars ( Arachis hypogaea L. IAPAR 25 Tição and IAC Tatu ST) grown under water regimes in order to verify drought tolerance. The randomized block experi­mental design was used in a 2 x 3 factorial design (two cultivars and three water regimes). The water regimes were, namely: control, in which plants were maintained at field capacity conditions (FC - without water deficit); moderate water deficit (MD - 50% of water deficit); and severe water deficit (SD - 75% of water deficit) in relation to control. Relative water content, gas exchange, dry matter, and leaf proline content were analyzed. Both cultivars developed physiological mechanisms of acclimatization to water deficit, such as reducing leaf relative water content, CO 2 assimilation rate, stomatal conductance, and transpiration in the initial period of water deficit, besides increasing proline production. Such physiological changes negatively affected dry matter accumulation in the grains and the number of grains, as well as dry matter accumulation in shoots and roots.

Quality of life and safety impact of COVID-19 associated smell and taste disturbances

ObjectiveThe association between COVID-19 and chemosensory loss has garnered substantial attention, however to date little is known about the real-life consequences of impairment in this unique patient population. The aim of this study is to evaluate the quality of life (QOL) and personal safety deficits experienced by patients with COVID-19 infection. Study designProspective, longitudinal questionnaires. SettingNational survey. MethodsA longitudinal web-based nationwide survey of adults with COVID-19 and/or a sudden change in smell and taste was launched April 10, 2020. Previously published questions on chemosensory-related QOL and safety events were asked at the 6-month follow-up survey. ResultsAs of February 10, 2021, 480 eligible respondents took the 6-month questionnaire, of whom 322 were COVID-19 positive. Impact on QOL was substantial with 96% of subjects reporting at least one of the defined deficits, and over 75% reporting at least 3 of these. “Reduced enjoyment of food” was the most common complaint (87%), while 43% of subjects self-reported depression. The prevalence of safety-related issues was common in this population, with over 57% reporting at least one, and 36% reporting 2 or more events. Of the events asked, the inability to smell smoke that others could perceive was the most common at 45%. ConclusionsCOVID-19 associated chemosensory losses have a real and substantial impact on both quality of life and safety, beyond mere inconvenience. The high prevalence of these issues despite a relatively short period of olfactory deficit should alert clinicians to the serious risks to an already vulnerable patient population.

Association of Body Condition Score with Ultrasound Measurements of Backfat and Longissimus Dorsi Muscle Thickness in Periparturient Holstein Cows

Simple SummaryAssociation of body condition scoring with the amount and mobilization of backfat and skeletal muscle around calving in dairy cows, although essential, has not been sufficiently investigated yet. The metabolic state of subcutaneous fat can be safely predicted by repeated body condition scorings, but the metabolic state of muscle tissue must be assessed by repeated ultrasound measurements. The results enhance our on-farm ability to assess the nutrient reserves in dairy cows transitioning from gestation to lactation.Most cows experience a period of nutrient deficit during the periparturient period. Body condition scoring (BCS) is widely used on farms to assess body nutrient reserves and mobilization. The aims of this study were to: (i) determine the association of BCS with ultrasound measurements of backfat (BFT) and longissimus dorsi muscle thickness (LDT) during the periparturient period of Holstein cows from different herds, accounting for potential sources of variation, such as herd, parity and period relative to calving and (ii) establish reference intervals (RIs) for BFT and LDT per BCS estimate. Two-hundred and fifty-two cows from six commercial farms were used. Body condition scores, BFT and LDT were assessed at seven time-points during the periparturient period. Assessments of BCS estimates as predictors of BFT and LDT and the contribution of BFT and LDT to BCS estimates were performed with the use of linear mixed models. Reference intervals for BFT and LDT per BCS estimate were established with the Reference Value Advisor. One unit of BCS change was associated with 8.2 mm of BFT and 10.9 mm of LDT pre- and postpartum. Range of BFT and LDT in established RIs per BCS was wide with significant overlap. Both subcutaneous fat and, to a lesser degree, skeletal muscle reserves contribute to BCS estimation. Repeated BCS estimations credibly predict energy balance status in periparturient dairy cows. The metabolic state of muscle tissue should be assessed by repeated ultrasound measurements.

Mitochondrial bioenergetic pathways in blood leukocyte transcriptome decrease after intensive weight loss but are rescued following weight regain in female physique athletes.

Prolonged periods of energy deficit leading to weight loss induce metabolic adaptations resulting in reduced energy expenditure, but the mechanisms for energy conservation are incompletely understood. We examined 42 healthy athletic females (age 27.5±4.0years, body mass index 23.4±1.7kg/m2 ) who volunteered into either a group dieting for physique competition (n=25) or a control group (n=17). The diet group substantially reduced their energy intake and moderately increased exercise levels to induce loss of fat mass that was regained during a voluntary weight regain period. The control group maintained their typical lifestyle habits and body mass as instructed. From the diet group, fasting blood samples were drawn at baseline (PRE), after 4- to 5-month weight loss (PRE-MID), and after 4- to 5-month weight regain (MID-POST) as well as from the control group at similar intervals. Blood was analyzed to determine leukocyte transcriptome by RNA-Sequencing and serum metabolome by nuclear magnetic resonance (NMR) platform. The intensive weight loss period induced several metabolic adaptations, including a prominent suppression of transcriptomic signature for mitochondrial OXPHOS and ribosome biogenesis. The upstream regulator analysis suggested that this reprogramming of cellular energy metabolism may be mediated via AMPK/PGC1-α signaling and mTOR/eIF2 signaling-dependent pathways. Our findings show for the first time that prolonged energy deprivation induced modulation of mitochondrial metabolism can be observed through minimally invasive measures of leukocyte transcriptome and serum metabolome at systemic level, suggesting that adaptation to energy deficit is broader in humans than previously thought.

Studi Analisa Kekeringan dengan Metode Standardized Precipitation Index (SPI) dan Palmer Drought Severity Index (PDSI) di DAS Kemuning Kabupaten Sampang

Drought is a natural disaster that occurs slowly but harms Sampang Regency people's life sustainability. Consider the negative impact of drought, analyze the drought index and its distribution are necessary. This study aims to know drought level using Standardized Precipitation Index (SPI) and Palmer Drought Severity Index (PDSI), then to know their correlation with Southern Oscillation Index (SOI) that can represent El Nino Southern Oscillation (ENSO). Drought index with bigger correlation mapped by Inverse Distance Weighting method to know the distribution. SPI method produced the most severe drought in April 2004 on one month deficit period (-3.651). PDSI method developed the most severe drought in September 2001 (-20.628). Based on the PDSI average analysis results for the 1998-2017 period, it is well-known that drought disasters generally start in July and end in October, while the peak of drought occurs in September. The PDSI method has 60% conformity to the SOI value derived from the graphical visualization of the annual average surplus and deficit index, better than the SPI method that only scores 53%. The visualization of the drought distribution map shows that the Districts of Sampang, Torjun, and Camplong need to become prioritized to mitigate future drought disasters because they have a great potential for drought disasters compared to other districts.