Abstract
Yarrowia lipolytica is one of the most studied non-conventional forms of yeast, exhibiting a high secretory capacity and producing many industrially important and valuable metabolites. The yeast conceals a great biotechnological potential to synthesize organic acids, sweeteners, microbial oil, or fragrances. The vast majority of bioprocesses are carried out in bioreactors, where suitable culture conditions are provided. In the current study, the effect of agitation speed (200–600 rpm) and air flow rate (0.0375–2.0 dm3/(dm3 × min)) on the biomass yield and lipase activity of Y. lipolytica KKP 379 is analyzed in a growth medium containing waste fish oil. The increase of aeration intensity limited the period of oxygen deficit in the medium. Simultaneously, an increase in lipolytic activity was observed from 2.09 U/cm3 to 14.21 U/cm3; however, an excessive agitation speed likely caused oxidative or shear stresses, and a reduction in lipolytic activity was observed. Moreover, it is confirmed that the synthesis of lipases is related to oxygen consumption, pH, and the yeast growth phase, and appropriate process selection may provide two advantages, namely, the maximum use of the waste carbon source and the production of lipolytic enzymes that are valuable in many industries.
Highlights
Lipases are widespread in nature and are synthesized by plants, animals, microorganisms, and even viruses [1,2]
The current study aims to analyze the effect of bioreactor culture conditions on the growth of biomass and the ability to biosynthesize extracellular lipolytic enzymes for Yarrowia lipolytica KKP 379
Chemicals were purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland) and Sigma-Aldrich (Poznań, Poland). p-Nitrophenyl laurate for lipase activity measurements was synthesized in the Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences-SGGW [24]
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Lipolytic enzymes (EC 3.1.1.3) belong to the hydrolase family and are responsible for the hydrolysis of triglycerides to di- and mono-glycerides, fatty acids, and glycerol. Lipases are widespread in nature and are synthesized by plants, animals, microorganisms, and even viruses [1,2]
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