Time Period Research Articles

Research on the duplication and death probability of the DNA of Escherichia coli within different generational time periods

Since the discovery of Escherichia coli during the 17th century, scientists have made substantial efforts to investigate the trends in the advanced combination probability of the Escherichia coli culture. This paper puts forward a combination method for models, via Escherichia coli. Here this paper develops a Markov-chain model for its population in different generational time periods and predicts the chance for duplication and loss from their stationary phase. Understanding the Duplication rate of Escherichia coli is crucial for various fields, including microbiology, medicine, and biotechnology. Our hypothesis believes that there is a correlation between the generational time intervals and the probability of Escherichia coli l duplication. This research contributes to our understanding of its Duplication dynamics and provides valuable information for various applications including the development of antimicrobial strategies, optimization of Escherichia coli l growth in biotechnological process, and the study of its evolution.

Spillover effects, lead and lag relationships, and stable coins time series

This research explores plausible spillover effects among S&P 500 Index, stable coins, and selected cryptocurrency time series by examining observable lead and lag interrelationships among the series. Considering the heteroscedastic and “fat-tailed” nature of the data distributions underlying the empirical analyses, we employ quantile Granger Causality tests to improve the validity of our statistical findings. Our test results suggest that stable coins, USDT, and USDC offer diversification benefits by decreasing portfolio risk. The log returns of the S&P 500 Index, stable coins, Bitcoin, Ethereum, and Binance coins demonstrate clear bidirectional causality and spillover effects in low and high quantiles. Interestingly, however, stable coin and USDT returns strongly lead S&P 500 Index returns in nearly all quantiles for post COVID-19 time periods. These findings indirectly support intuition based upon market co-movement or integration assertions and suggest that investors can obtain added diversification benefits deriving from causality or spillover effects of holding stable coins when forming investment portfolios.

ON THE PERFECTION OF THE APPROACH TO THE FORMATION OF A PROGRAM FOR INCREASING THE RELIABILITY OF FIELD PIPELINES IN OC ROSNEFT

The article describes an approach for ranking and selecting field pipelines based on the total cost of ownership of the facility for the purpose of timely repairs and minimization of accidents, as well as its software implementation. The principle of the proposed approach is to apply a technical and economic model of the total cost of ownership of a pipeline, which takes into account the costs of maintaining the pipeline over a long period of time, which include the costs of pipeline maintenance activities, pipeline failures and replacement of pipeline sections. This model allows determining which replacement year corresponds to the minimum accumulated maintenance costs, by varying the year of pipeline replacement from the start of operations to the planning horizon. The model also allows to take into account the impact of operational technological measures on the cost of pipeline ownership and the selection of the optimal year of repair.In order to take into account the level of pipeline accidents in the process of operation on the basis of operational data, a mathematical probabilistic model based on the Weibull distribution was developed. The mathematical model allows to take into account for each pipeline the influence of technical characteristics (diameter, length, wall thickness, material, coating, design features), operating conditions (type of pumped medium, technological mode of pipeline operation) and information on inspection and diagnostics. The accuracy of failure prediction using this model is at least 90 %.The approach to the formation of programs to improve the reliability of field pipelines with regard to optimal repair periods based on the total cost of ownership model is described, taking into account various objectives and constraints, among which the main ones are:- required achievable level of accidents;- acceptable amounts of financing for re-pairs;- permissible physical volumes of repairs along the length.The approach to forming reliability improvement programs described in this article has been implemented in Rosneft's corporate software system RN-SM. The implementation of this software package in production activities is being implemented in 2023–2024.

A pilot protection based on low frequency component model for HVDC transmission lines

AbstractReliable and fast identification of high‐voltage direct current (HVDC) transmission line faults is important to the safety of power system. In traditional DC transmission line protection, differential protection is invulnerable to high fault resistance, but it needs long time delay for the influence of distributed capacitance current. In order to solve this problem, a novel pilot protection is proposed. The characteristics of the voltage and current processed by low‐pass filter under different fault conditions are analysed, and based on it the protection criteria is proposed. When external fault occurs, it reflects the line capacitance where the first forward fault traveling wave passes, which is increasing until equal to the whole line capacitance, then remains constant. However, when internal fault occurs, its value is negative in the initial stage, and after that its value is very different from the whole line capacitance. The analysis proves that this pilot protection method has fast operation speed, and can be applied for a relatively long period of time. What is more, the applicable time of superposition principle is considered in the analysis, which is not considered in many literatures. An UHVDC system is modelled for simulation in PSCAD/EMTDC, and the correctness and effectiveness of the proposed scheme is verified.

A 30 YEAR (1991-2020) RETROSPECTIVE MORTALITY REVIEW OF GIRAFFIDS (GIRAFFA SPP. AND OKAPIA JOHNSTONI) IN MANAGED CARE.

Identifying common causes of mortality in zoo giraffe (Giraffa spp.) and okapi (Okapia johnstoni) provides an opportunity to help improve welfare and population management for these endangered species. Mortality reports from 1,024 giraffe and 95 okapi in zoos were compiled from the Species 360 Zoological Information Management Software (ZIMS) utilizing the Morbidity & Mortality Analysis tool. Thirty years of mortality reports (1991-2020) were evaluated to help identify trends and evaluate the impacts, if any, of changes over time in husbandry and management practices. The most common causes of death for giraffe from 1991 to 2015 were neonatal issues (234/845, 27.7%), trauma (213/845, 25.2%), noninfectious disease (190/845, 22.5%), and infectious disease (188/845, 22.2%). In comparison, the most common causes of mortality for giraffe from 2016 to 2020, were noninfectious disease (78/179, 43.6%), trauma (39/179, 21.8%), neonatal issues (39/179, 21.8%), and infectious disease (17/179, 9.5%). The most common cause of death for okapi from 1991 to 2015 were neonatal issues (29/64, 45.3%), infectious disease (13/64, 20.3%), noninfectious disease (11/64, 17.2%), and trauma (10/64, 15.6%). In comparison, the most common cause of death for okapi from 2016 to 2020 was noninfectious disease (15/31, 48.4%), neonatal issues (8/31, 25.8%), and infectious disease (5/31, 16.1%). The results suggest that zoo giraffids have had a relative decrease in mortality from infectious diseases in recent years, whereas death from noninfectious causes has increased significantly. Trauma-related giraffe mortalities and neonatal mortality in both giraffe and okapi, although decreasing in prevalence between time periods, continue to be important causes of death in zoos. This is the first descriptive mortality review for the Giraffidae family and provides data on potential giraffe and okapi health issues that zoos could proactively address.

Long‐term differences in population structure, body size and distribution patterns of amphipods and isopods in wetlands with declining hydroperiods

Abstract In many regions, climatic drying is shortening hydroperiods and freshwater biodiversity is declining. Aquatic species that lack a desiccation‐resistant life stage are predicted to have the least ability to persist in drying climates, yet such species may occur in intermittent waterbodies. We examined the distribution of two crustacean species that lack desiccation‐resistant life stages: the isopod Paramphisopus palustris and amphipod Chiltoniidae sp.nov., before and after the commencement of severe climatic drying in Swan Coastal Plain wetlands, south‐western Australia. Historical distribution data for these species were obtained from studies of the same set of wetlands in 1989–1990. We determined whether population or body size differed between hydrological regimes (perennial, semi‐perennial, seasonal) between 1989–1990 and 2018–2019 for both species. Amphipods, isopods and environmental variables were sampled from 42 wetlands in 2018–2019. Thirty‐three wetlands (22 perennial, 11 seasonal) persisted with the same hydrological regime in both time periods and eight wetlands that were perennial in 1989–1990 had shifted to a drier hydrological regime (four became semi‐perennial, four became seasonal) by 2018–2019. Drying wetland hydrological regimes did not correspond to more limited spatial distributions for either species. Hydrological regime had no effect on amphipod counts, although abundances were lower in 2018–2019 than 1989–1990. In 2018–2019, male amphipods were larger than females in seasonal wetlands, but sexes were the same size in perennial wetlands and females were larger than males in semi‐perennial wetlands. Furthermore, amphipods of both sexes were considerably smaller in semi‐perennial than seasonal or perennial wetlands. Isopod distributions differed between the two sampling periods, with populations primarily lost from wetlands that remained perennial. This unexpected result was likely caused by prolonged periods of high summer–autumn water temperatures and stratification in shallow perennial wetlands that caused anoxia in bottom waters and made surface waters excessively hot for isopods. By contrast, drying seasonal wetlands seemingly offered suitable habitat in summer–autumn. Mean isopod head and body length did not differ between hydrological regimes or sexes, but became smaller between 1989–1990 and 2018–2019 (15 mm average body length in 1989 vs. 8.3 mm in 2018–2019; 17 mm maximum size in 1989–1990 vs. 13.2 mm in 2018–2019). The absence of population losses associated with drying hydrological regimes indicates that the current level of drying is within the tolerance range of both species. However, increased temperatures may be causing body size (and possibly fecundity) to decline for isopods. As global warming continues to shorten wetland hydroperiods in many regions, numerous species will struggle to complete their life cycles, leading to extirpation. Our findings suggest that climate change may also cause conditions in perennial wetlands to exceed the tolerances of species that lack desiccation resistance traits. This emphasises the need to better understand both environmental and habitat changes along the drying trajectory in wetlands and the fundamental life‐history and physiological traits that enable the survival of aquatic invertebrates that lack desiccation resistance. This includes not only species responses to drying, but also responses to other stressors (e.g., prolonged stratification) caused by global warming.

Kilometre-scale simulations over Fennoscandia reveal a large loss of tundra due to climate warming

Abstract. The Fennoscandian boreal and mountain regions harbour a wide range of vegetation types, from boreal forest to high alpine tundra and barren soils. The area is facing a rise in air temperature above the global average and changes in temperature and precipitation patterns. This is expected to alter the Fennoscandian vegetation composition and change the conditions for areal land use such as forestry, tourism and reindeer husbandry. In this study we used a unique high-resolution (3 km) climate scenario with considerable warming resulting from strongly increasing carbon dioxide emissions to investigate how climate change can alter the vegetation composition, biodiversity and availability of suitable reindeer forage. Using a dynamical vegetation model, including a new implementation of potential reindeer grazing, resulted in simulated vegetation maps of unprecedented high resolution for such a long time period and spatial extent. The results were evaluated at the local scale using vegetation inventories and for the whole area against satellite-based vegetation maps. A deeper analysis of vegetation shifts related to statistics of threatened species was performed in six “hotspot” areas containing records of rare and threatened species. In this high-emission scenario, the simulations show dramatic shifts in the vegetation composition, accelerating at the end of the century. Alarmingly, the results suggest the southern mountain alpine region in Sweden will be completely covered by forests at the end of the 21st century, making preservation of many rare and threatened species impossible. In the northern alpine regions, most vegetation types will persist but shift to higher elevations with reduced areal extent, endangering vulnerable species. Simulated potential for reindeer grazing indicates latitudinal differences, with higher potential in the south in the current climate. In the future these differences will diminish, as the potentials will increase in the north, especially for the summer grazing grounds. These combined results suggest significant shifts in vegetation composition over the present century for this scenario, with large implications for nature conservation, reindeer husbandry and forestry.

When and how many: Factors associated with campus sexual assault reforms

AbstractInstitutions of higher education have faced increasing pressure to comply with federal regulations and reform their response to campus sexual assault. This study explores institutions of higher education employees’ perceptions on whether decoupling, or organizational resistance to change, is associated with the number and timing of campus sexual assault reforms adopted. Early captured reforms instituted before the “Dear Colleague Letter” in 2011, mid included reforms instituted after the Dear Colleague Letter in 2011 but before the 2015–2016 academic year and during the period of heightened attention to campus sexual assault, and late which included reforms instituted during or after the 2015–2016 academic year. A web‐based survey of institutions of higher education employees familiar with sexual assault policy implementation on their campuses asked about types of reforms, timing, decoupling, and campus characteristics. Correlations and t‐tests were run to examine the types of reforms across time periods, and regression assessed the degree to which decoupling was associated with the number and timing of reforms. Higher decoupling was associated with fewer reforms in the early period and more in the late period, though not with the overall number of reforms adopted. Findings highlight the importance of understanding factors that influence change on campuses.

Chest X-ray image classification using transfer learning and hyperparameter customization for lung disease diagnosis

ABSTRACT Lung diseases often result in severe damage to the respiratory tract, and lead to a high risk of mortality within a short period of time. DL models based on ViT are considered to have promising advantages over CNN architectures in terms of computational efficiency, and accuracy when trained on large ImageNet datasets. In this study, we present a new DL approach based on the combination of CNN with ViT to improve the efficiency of pneumonia diagnosis using medical images. In the first stage, raw images are passed through a local filter to capture local relations on the inputs. The local filter block includes two convolutional layers with kernel 3 × 3. This local filtering method aims to enhance rich features before being fed into the patching layer of the ViT block. The proposed method is experimented on the benchmark chest X-ray dataset. The proposed method is evaluated and compared to some well-known models, which include ViT, VGG19, Resnet50, Densnet201. Experimental results demonstrated that the proposed approach based on CNN and ViT reaches higher efficiency with about 1% accuracy to the standard ViT model, and about 2% higher with VGG19, Resnet50, Densnet201 and smaller in model architecture.

Comparison of the oncolytic activity of a replication-competent and a replication-deficient herpes simplex virus 1.

In 2015, the oncolytic herpes simplex virus 1 (HSV-1) T-VEC (talimogene laherparepvec) was approved for intratumoral injection in non-resectable malignant melanoma. To determine whether viral replication is required for oncolytic activity, we compared replication-deficient HSV-1 d106S with replication-competent T-VEC. High infectious doses of HSV-1 d106S killed melanoma (n = 10), head-and-neck squamous cell carcinoma (n = 11), and chondrosarcoma cell lines (n = 2) significantly faster than T-VEC as measured by MTT metabolic activity, while low doses of T-VEC were more effective over time. HSV-1 d106S and, to a lesser extent T-VEC, triggered caspase-dependent early apoptosis as shown by pan-caspase inhibition and specific induction of caspases 3/7, 8, and 9. HSV-1 d106S induced a higher ratio of apoptosis-inducing infected cell protein (ICP) 0 to apoptosis-blocking ICP6 than T-VEC. T-VEC was oncolytic for an extended period of time as viral replication continued, which could be partially blocked by the antiviral drug aciclovir. High doses of T-VEC, but not HSV-1 d106S, increased interferon-β mRNA as part of the intrinsic immune response. When markers of immunogenic cell death were assessed, ATP was released more efficiently in the context of T-VEC than HSV-1 d106S infection, whereas HMGB1 was induced comparatively well. Overall, the early oncolytic effect on three different tumour entities was stronger with the non-replicative strain, while the replication-competent virus elicited a stronger innate immune response and more pronounced immunogenic cell death.

Acknowledging and understanding the contributions of nature to human sense of time

Abstract There is growing evidence that interacting and connecting with nature are essential to maintain human health and well‐being. The benefits of specific nature experiences and the cultural ecosystem services they provide are increasingly being recognized, but many others remain to be discovered and explored. In this perspective piece, I argue that there is a need to better explore the pivotal role of nature experiences in shaping human time perception—our sense of time. Specifically, I outline the main elements of human time perception and the key factors that shape it, describe recent evidence showing that human time perception changes between urban and natural environments and discuss the potential societal gains from developing a better understanding of this relationship. Human time perception is complex and involves at least three key dimensions related to temporal succession, temporal duration and temporal perspective. Time perception is shaped by various contextual factors, including the contents of the time period and the cognitive, emotional and bodily characteristics of the experiencer. There is growing evidence that nature experiences can influence human sense of time by (i) extending human perception of temporal duration and (ii) shifting time perspectives. People who spend time in nature tend to overestimate the length of that experience and show a more positive outlook of the past, present and future, with less focus on a single‐time perspective. In the context of increasing time scarcity in modern urban societies with dire consequences for human health and well‐being, I argue we need a better understanding of how nature experiences shape our sense of time and suggest how future research can provide actionable insights to help restore a healthier and more balanced relationship with time and nature. Read the free Plain Language Summary for this article on the Journal blog.

Admission avoidance hospital at home.

Admission avoidance hospital at home provides active treatment by healthcare professionals in the patient's home for a condition that would otherwise require acute hospital inpatient care, and always for a limited time period. This is the fourth update of this review. To determine the effectiveness and cost of managing patients with admission avoidance hospital at home compared with inpatient hospital care. We searched the Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, Embase, and CINAHL on 24 February 2022, and checked the reference lists of eligible articles. We sought ongoing and unpublished studies by searching ClinicalTrials.gov and WHO ICTRP, and by contacting providers and researchers involved in the field. Randomised controlled trials recruiting participants aged 18 years and over. Studies comparing admission avoidance hospital at home with acute hospital inpatient care. We followed the standard methodological procedures expected by Cochrane and the Effective Practice and Organisation of Care (EPOC) Group. We performed meta-analysis for trials that compared similar interventions, reported comparable outcomes with sufficient data, and used individual patient data when available. We used the GRADE approach to assess the certainty of the body of evidence for the most important outcomes. We included 20 randomised controlled trials with a total of 3100 participants; four trials recruited participants with chronic obstructive pulmonary disease; two trials recruited participants recovering from a stroke; seven trials recruited participants with an acute medical condition who were mainly older; and the remaining trials recruited participants with a mix of conditions. We assessed the majority of the included studies as at low risk of selection, detection, and attrition bias, and unclear for selective reporting and performance bias. For an older population, admission avoidance hospital at home probably makes little or no difference on mortality at six months' follow-up (risk ratio (RR) 0.88, 95% confidence interval (CI) 0.68 to 1.13; P = 0.30; I2 = 0%; 5 trials, 1502 participants; moderate-certainty evidence); little or no difference on the likelihood of being readmitted to hospital after discharge from hospital at home or inpatient care within 3 to 12 months' follow-up (RR 1.14, 95% CI 0.97 to 1.34; P = 0.11; I2 = 41%; 8 trials, 1757 participants; moderate-certainty evidence); and probably reduces the likelihood of living in residential care at six months' follow-up (RR 0.53, 95% CI 0.41 to 0.69; P < 0.001; I2 = 67%; 4 trials, 1271 participants; moderate-certainty evidence). Hospital at home probably results in little to no difference in patient's self-reported health status (2006 patients; moderate-certainty evidence). Satisfaction with health care received may be improved with admission avoidance hospital at home (1812 participants; low-certainty evidence); few studies reported the effect on caregivers. Hospital at home reduced the initial average hospital length of stay (2036 participants; low-certainty evidence), which ranged from 4.1 to 18.5 days in the hospital group and 1.2 to 5.1 days in the hospital at home group. Hospital at home length of stay ranged from an average of 3 to 20.7 days (hospital at home group only). Admission avoidance hospital at home probably reduces costs to the health service compared with hospital admission (2148 participants; moderate-certainty evidence), though by a range of different amounts and using different methods to cost resource use, and there is some evidence that it decreases overall societal costs to six months' follow-up. Admission avoidance hospital at home, with the option of transfer to hospital, may provide an effective alternative to inpatient care for a select group of older people who have been referred for hospital admission. The intervention probably makes little or no difference to patient health outcomes; may improve satisfaction; probably reduces the likelihood of relocating to residential care; and probably decreases costs.

The straw that broke the nurse's back-Using psychological contract breach to understand why nurses leave.

To deepen our understanding of why nurses decide to leave their occupation instead of changing jobs, we examined the antecedents that led to this decision through the theoretical lens of psychological contract breach. A qualitative design with semi-structured interviews. We collected 28 interviewees from our social networks and a social media platform. We included professional nurses who had decided to leave or had left the occupation. We analysed our data with reflexive thematic analysis, thereby giving space for the interviewee voices to rise. We identified various experiences of dissonance between interviewee expectations and reality. The interviewees expressed unfulfilled expectations or psychological contract breaches in relation to their occupation on different levels and over extended time periods. The psychological contract breaches and decisions to leave the occupation were built up over time because of continuous disappointment and dissonance between expectations and reality. The frustration, dissonance and unfulfilled expectations were expressed towards the institution of nursing rather than a specific employer or organization. Unfulfilled expectations over a longer period might cause psychological contract breach, leading to turnover intentions. Our research brings novel insights into the psychological contract, as our findings indicate that the psychological contract can be formed and breached, also between the employee and the occupation. This means that turnover intentions might result in nurses leaving the profession rather than seeking work in new organizations. The study addresses the problem of nurse shortage by showing the root reasons for deciding to leave the occupation. Our findings show how psychological contract breaches over time erupt as turnover intentions regarding the occupation rather than a job. The results guide healthcare managers and decision-makers to recognize factors leading to a psychological contract breach, thereby enabling the retention of nurses. No patient or public contribution.

Tourism Development and Multidimensional Poverty Alleviation

Tourism development is often used as a strategy to fight poverty, but in the tourism literature it remains controversial as a tool for poverty alleviation in a poor region. This study adopts a multidimensional framework to measure regional poverty. Specifically, we examine four dimensions, including economics, education, social security, and medical service, to reflect poverty alleviation for a region. Then we establish econometric models to explore the link between tourism and poverty alleviation. The result shows that tourism development has a positive influence on multidimensional poverty alleviation in 73 counties in Guizhou province, a typically poor region in China. The consideration of the multidimensional nature of poverty in this study is more convictive to conclude that tourism can reduce poverty. This study further demonstrates that political pressure, indicated by the time period near the end of the fight-against-poverty campaign and by whether a county has red-culture heritages, affects tourism-led poverty alleviation in China.

Surface Albedo Feedback, Seasonal Heat Storage and Meridional Heat Transport Determine the Seasonality of Recent Warming in Antarctica

AbstractThe reanalysis data suggest that recent surface warming over Antarctica start in 2016. In this study, using reanalysis data and numerical simulations, I attempt to determine the important mechanisms accounting for seasonal surface warming in Antarctica. The results suggested that seasonal surface warming in Antarctica is mainly determined by the surface energy budget over the Antarctic via horizontal heat advection. The surface energy budget anomaly over the Antarctic, which is mainly determined by anomalous solar radiation absorption, anomalous ocean heat content, and anomalous meridional atmospheric heat transport (AHT), is triggered by Antarctic sea‐ice loss and thus determines the observational seasonality of recent warming in Antarctica via surface horizontal heat advection. In austral summer (December–January–February), additional solar radiation absorption induced by sea‐ice loss and additional AHT from lower latitudes increase the energy budget over the Antarctic. Surface warming, more longwave radiation, and additional energy stored in the upper (deeper) ocean for short (long) time periods explain the additional energy sinks. During austral autumn‐winter (March–August), additional seasonal heat storage (SHS; mainly stored in the upper ocean) is released to the atmosphere and warms the surface. Although the AHT anomaly contributes similarly to the solar radiation absorption/SHS anomaly during April–August, the poleward AHT largely decreased in June due to the weaker eddy activity induced by strong warming at Southern Hemisphere midlatitudes, which counteracts the additional SHS release and cools the Antarctic(a).

Development at the cost of unsustainable degradation of wetlands: Unraveling the dynamics (historic and future) of wetlands in the megacity Dhaka

Despite the recognized role of wetlands in providing ecological benefits for human wellbeing, ∼70% of global wetland ecosystems have been destroyed since the 1990s. Further intensive studies revealed that 3.4 million km2 of natural wetland has declined since 1700. In particular, wetland habitats in the world's megacities have been replaced unsustainably by faster economic, urban, and population growth, and have received less attention in research and policy. However, wetlands degradation in the megacities of developing countries is not quantified and the trends of Land Surface Temperature (LST) are not well understood. Therefore, we are making our first attempt to unravel the historical and future spatiotemporal dynamics of wetlands and the trends of LST in the megacity of Dhaka. The results show that Dhaka lost ∼69% of wetlands and LST has increased between 3.44°C and 9.35°C from 1990 to 2020. An environmental Kuznets curve analysis implies that the point has not yet been reached for wetlands when economic development feeds back to the sustainability of the environment. This assumption coincides with our model-based prediction, as respectively ∼74% and ∼90% of wetlands area of Dhaka city will be decreased by 2050 in Business as Usual (BAU) and development scenarios, whereas, ∼66% of wetlands area will be decreased under conservation scenario over the time period of next 30 (2020 to 2050) years. Our findings suggest that it will be incredibly challenging to restore wetlands to their 1990s condition. Efforts to preserve them should be made, as they potentially provide a nature-based alternative for coping with wetland sustainability and climate change.

Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B

Temperature is a vital environmental factor affecting organisms’ survival as they determine the mechanisms to tolerate rapid temperature changes. We demonstrate an experimental system for screening chemicals that affect cold tolerance in Caenorhabditis elegans. The anticancer drugs leptomycin B and camptothecin were among the 4000 chemicals that were screened as those affecting cold tolerance. Genes whose expression was affected by leptomycin B or camptothecin under cold stimuli were investigated by transcriptome analysis. Abnormal cold tolerance was detected in several mutants possessing genes that were rendered defective and whose expression altered after exposure to either leptomycin B or camptothecin. The genetic epistasis analysis revealed that leptomycin B or camptothecin may increase cold tolerance by affecting a pathway upstream of the insulin receptor DAF-2 that regulates cold tolerance in the intestine. Our experimental system combining drug and cold tolerance could be used for a comprehensive screening of genes that control cold tolerance at a low cost and in a short time period.

Test-retest reliability of a mobile application of the patient reported outcomes burdens and experiences (PROBE) study.

The Patient Reported Outcomes, Burdens, and Experiences (PROBE) questionnaire is a patient-reported outcome tool that assesses quality of life and disease burden in people with haemophilia (PWH). To assesses the test-retest reliability of PROBE when completed using the mobile phone application. We recruited PWH, including carriers, and individuals with no bleeding disorders who attended haemophilia-related workshops or via social media. Participants completed PROBE three times (twice on the app: T1 and T2, and once on the web, T3). Test-retest reliability was analysed for T1 versus T2 (app to app, time period one) and T2 versus T3 (app to web, time period two). We enrolled 48 participants (median age=56 [range 27-78] years). Eighteen participants (37.5%) were PWH and seven (14.6%) were carriers. On general health domain questions, we found almost perfect agreement, except for a question on the frequency of use of pain medication in the last 12 months [Kappa coefficient (κ).72 and.37 for time period one and two, respectively] and any use of pain medications (κ.75) for time period two. For haemophilia-related questions, we found substantial to perfect agreement, except for the questions on the number of joint bleeds in the previous 6 months for time period one (κ.49) and the number of bleeds in the previous two weeks for time period two (κ.34). The results demonstrate the reliability of the PROBE app. The app can be used interchangeably with the paper and web platforms for PROBE administration.

De Novo Design of a Highly Stable Ratiometric Probe for Long-Term Continuous Imaging of Endogenous HClO Burst.

Long-term continuous imaging of endogenous HClO burst is of great importance for the elucidation of various physiological or pathological processes. However, most of the currently reported HClO probes have failed to achieve this goal due to their insufficient photobleaching resistance under a laser source. Herein, a highly stable ratiometric probe, HFTC-HClO 1, which is capable of continuously monitoring endogenous HClO burst over a long period of time, has been judiciously developed. Briefly, the de novo development of HFTC-HClO 1 mainly involved three main steps: (1) novel coumarins (HFTC 1-5) were designed and synthesized; (2) the most stable scaffold, HFTC 3, was selected through dye screening and cell imaging validation; and (3) based on HFTC 3, three candidate HClO probes were constructed, and HFTC-HClO 1 was finally selected due to its superior sensing properties toward HClO. Furthermore, HFTC-HClO 1 can quantitatively measure HClO levels in various real samples with excellent recovery (>90.4%), and the use of HFTC-HClO 1-coated test strips for qualitative analysis of HClO in real samples was also achieved. In addition, the application of HFTC-HClO 1 for long-term continuous monitoring of intracellular HClO burst was successfully demonstrated. Significantly, HFTC-HClO 1 was able to visualize HClO generated in the rheumatoid arthritis mouse model.

Economic Depression and Recovering from the Trauma of the World War: The Social Reconstruction of Britain after World War II

The passage focuses on the reconstruction of Britain after World War II. The background of the research was the time period after WWII. In order to analyze the situation more precisely, the background information of the research had a time span from the 1920s to approximately the 1950s, starting from the Roaring Twenties to the publication of the Green Belt Policy. The passage focuses on the reorganization of cities and the reconstruction of the welfare system. The two examples listed in the following passage are the Green Belt Policy and the NHS, which represents the reconstruction of cities in England and the reconstruction of the welfare system of England. Other examples include data recorded in 1931 and several government documents by the British government. By answering the question of the advantages of Britain in the reconstruction of World War II, the passage hopes to provide some characteristics of the successful reconstruction of Britain to help reconstruction in other countries like Palestine.