In this paper, we describe the variation in myctophid schools characteristics at South Georgia using multi-frequency acoustic data collected annually between November 2007 and January 2012. We studied the relationship between the proximity of land and the distribution and schooling characteristics of myctophid fish. We also examined patterns in schooling behaviour in relation to oceanographic data. Fish schools were identified using a dual-frequency dB identification method (Sv120−38kHz), where negative Sv120−38kHz is indicative of gas-bearing organisms, such as swimbladdered fish. Available net data were used to provide information on the meosopelagic fish community in the region. School morphometrics (e.g. length, thickness, area) data were extracted and pooled according to their distance from the shore (0–30km, 30–60km, 60–90km, 90–120km). A total of 578 schools were detected in the survey region and there was high variation in school backscattering strength (NASC), length, height, perimeter, depth and horizontal distribution between surveys, including distinct inter-annual variation in these parameters when surveys were conducted in the same season (2009–2012). Schools were distributed predominantly on-shelf (0–500m isobaths) during some summer surveys (2007 and 2012) and predominantly off-shelf on others (>500m isobaths; 2009, 2010 and 2011). There was also evidence of bathymetric separation in the horizontal distribution of schools. Schools observed in the late-season in 2008 had the greatest NASC, largest height and the deepest depth distribution. Most schools occurred at depths between ∼60 and 300m, but they did not occupy the same water masses during each survey. Schools became progressively thinner, shallower and acoustically weaker with increasing distance to land, whilst school length and perimeter increased correspondingly. The change in fish schooling behaviour between environments could be a response to a combination of local predatory threats over short spatial/temporal scales and differences in oceanographic conditions, such as current velocity. The trend could also be evidence of spatial habitat partitioning of myctophids, with different schooling species, or different life-stages, orientating along a bathymetric gradient at South Georgia.