Performance Profile Research Articles

Effects of feeding rumen-protected lysine during the postpartum period on performance and amino acid profile in dairy cows: A meta-analysis

Lysine is one of the limiting AA in the diets of dairy cows and is typically fed as rumen-protected Lys (RPL). We hypothesized that supplementation of RPL during the postpartum period would improve the productive performance in dairy cows. Objectives were to use meta-analytic methods to explore the effects of feeding RPL on performance and blood AA profile in lactating dairy cows. An additional objective was to identify an optimal concentration (%) of Lys in MP (LYSMP) and determine if responses to LYSMP were associated with the concentration (%) of Met in MP (METMP). The literature was systematically reviewed, and 13 experiments, comprising 40 treatment means and 594 lactating cows, were included in the meta-analysis. All experiments had a nonsupplemental control (CON; n = 17 treatment means), or a group supplemented with RPL (n = 23 treatment means). Cows supplemented with RPL were supplied additionally with a mean (±standard deviation) 19.3 ± 10.3 g/d metabolizable Lys (5.1-40.6 g/d). Meta-analytical statistics were used to estimate the weighted mean difference in STATA. Mixed models were fitted to the data to investigate the linear and quadratic effects of LYSMP, METMP, and interactions between LYSMP and METMP. All models included the random effect of experiment and weighting by the inverse of the SE of the means squared. Cows that began receiving RPL in early lactation (≤90 DIM) or for an extended duration (≥70 DIM) produced 1.51 kg/d more milk compared with CON cows. Increasing digestible LYSMP from 6.5% to 8.5% linearly increased yields of milk, FCM, ECM, and milk fat by 1.8, 2.5, 2.4, and 0.10 kg/d, respectively, and tended to increase milk protein yield and body weight gain by 0.07 and 0.09 kg/d, respectively, without a concurrent increase in DMI. Interactions between the linear effects of LYSMP and METMP were observed for FCM/DMI or ECM/DMI. In a diet with low METMP (e.g., 1.82% of MP), a digestible supply of 7.40% LYSMP would result in 1.46 and 1.47 kg/kg FCM/DMI or ECM/DMI, respectively; however, with high digestible METMP (e.g., 2.91% of MP), supplying 7.40% of digestible LYSMP would result in 1.68 and 1.62 kg/kg FCM/DMI or ECM/DMI, respectively. Increasing digestible LYSMP from 6.5% to 8.5% linearly increased blood concentrations of Lys by 16.6 µM, whereas blood concentrations of Met and Ala decreased by 4.6 and 6.0 µM, respectively. Nevertheless, an interaction was also observed between LYSMP and METMP for blood concentrations of total EAA because as METMP increased, the positive response to LYSMP on total EAA was also increased, suggesting a competitive mobilization of AA and their utilization in various body tissues. Only 4 out of the 13 experiments in this meta-analysis involved primiparous cows; thus, insufficient data were available to understand the role of supplemental RPL in primiparous cows. Collectively, feeding RPL improved productive performance, and the increments were maximized up to 9.25% of LYSMP in multiparous dairy cows.

Patients with first-episode psychosis in northern Taiwan: neurocognitive performance and niacin response profile in comparison with schizophrenia patients of different familial loadings and relationship with clinical features

BackgroundExamining patients with first-episode psychosis (FEP) provides opportunities to better understand the mechanism underlying these illnesses. By incorporating quantitative measures in FEP patients, we aimed to (1) determine the baseline distribution of clinical features; (2) examine the impairment magnitude of the quantitative measures by comparing with external controls and then the counterparts of schizophrenia patients of different familial loadings; and (3) evaluate whether these quantitative measures were associated with the baseline clinical features.MethodsPatients with FEP were recruited from one medical center, two regional psychiatric centers, and two private clinics in northern Taiwan with clinical features rated using the Positive and Negative Syndrome Scale (PANSS) and Personal and Social Performance (PSP) scale. Quantitative measurements included the Continuous Performance Test (CPT), Wisconsin Card Sorting Test (WCST), niacin response abnormality (NRA), and minor physical anomalies and craniofacial features (MPAs). To evaluate the relative performance of the quantitative measures in our FEP patients, four external comparison groups from previous studies were used, including three independent healthy controls for the CPT, WCST, and NRA, respectively, and one group of treatment-resistant schizophrenia patients for the MPAs. Additionally, patients from simplex families and patients from multiplex families were used to assess the magnitude of FEP patients’ impairment on the CPT, WCST, and NRA.ResultsAmong the 80 patients with FEP recruited in this study (58% female, mean age = 25.6 years, mean duration of untreated psychosis = 132 days), the clinical severity was mild to moderate (mean PANSS score = 67.3; mean PSP score = 61.8). Patients exhibited both neurocognitive and niacin response impairments (mean Z-scores: −1.24 for NRA, − 1.06 for undegraded d', − 0.70 for degraded d', − 0.32 for categories achieved, and 0.44 for perseverative errors) but did not show MPAs indicative of treatment resistance. Among these quantitative measures, three of the four neurocognitive indices were correlated with the baseline clinical features, whereas NRA did not show such correlation.ConclusionsThis FEP study of Taiwanese patients revealed the presence of neurocognitive performance and niacin response and their different relationships with clinical features, rendering this sample useful for future follow-up and incorporation of multiomics investigation.

Hybrid Modified Accelerated Gradient Method for Optimization Processes

This research reveals a hybrid variant of the modified accelerated gradient method. We prove that derived iteration is linearly convergent on the set of uniformly convex functions. Performance profiles of the introduced hybrid method were numerically compared with its non-hybrid version. The analyzed characteristics were CPU time, the number of iterations and the number of function evaluations. The results of the numerical experiments show a better performance in favor of the derived hybrid accelerated model compared with its forerunner.

A multilevel investigation of factors related to achievement in Ireland and Spain using PISA data

The Program for International Assessment (PISA) is a methodology for making comparative judgments about the quality of education systems. Celebrated by proponents as a transparent process that allows policy makers produce data informed judgments about relative quality of their national education system PISA – and through it the OECD – has become a key vehicle for informing and explaining educational policy development. This paper explores the Irish and Spanish outcomes of the 2018 round of PISA. It examines the contextual factors that are associated with performance at student and school level while at the same time developing a multi-level statistical model to explain divergent school performance profiles It finds that issues associated with the socio-economic level of the students, the repetition rate, and student age are common across all domains in both countries. It suggests that the socio-economic status of Spanish students at school level is not significant, that the shortage of teachers in Ireland affects student performance, and that immigrant status does not disadvantage Spanish student performance. It concludes by suggesting that studies involving a wider application of the model be undertaken to assess possible social, economic, and cultural causes that may explain the differences in variable significance in each country.

Bacillus amyloliquefaciens Probiotics Mix Supplementation in a Broiler Leaky Gut Model.

The supplementation of antimicrobial growth promoters (AGPs) has been banned in many countries because of the emergence of antimicrobial-resistant pathogens in poultry products and the environment. Probiotics have been broadly studied and demonstrated as a promising AGP substitute. Our study is centred on the effects of a multi-strain Bacillus-based probiotic product on broiler production performance and gut microbial profile in a dexamethasone-induced leaky gut challenge. Two hundred and fifty-six broiler chicks were hatched and randomly assigned into four groups (wheat-soybean meal basal diet (BD) = non-supplemented control (C), BD supplemented with dexamethasone in week 4 (CD), BD containing a probiotic from day one (P), and BD containing a probiotic from day one and supplemented with dexamethasone during challenge week 4 (PD)). The production performance and caecal, gizzard, jejunal lumen and jejunal mucosa swab microbiota were studied by 16S rRNA gene sequencing. The Bacillus probiotic product significantly improved production performance and altered caecal gut microbiota (p ≤ 0.05), but no significant impact on microbiota was observed in other gut sections.

Engineering Features from Raw Sensor Data to Analyse Player Movements during Competition.

Research in field sports often involves analysis of running performance profiles of players during competitive games with individual, per-position, and time-related descriptive statistics. Data are acquired through wearable technologies, which generally capture simple data points, which in the case of many team-based sports are times, latitudes, and longitudes. While the data capture is simple and in relatively high volumes, the raw data are unsuited to any form of analysis or machine learning functions. The main goal of this research is to develop a multistep feature engineering framework that delivers the transformation of sequential data into feature sets more suited to machine learning applications.

Organizational changes and research performance: A multidimensional assessment

Abstract This paper analyzes the research performance evolution of a scientific institute, from its genesis through various stages of development. The main aim is to obtain, and visually represent, bibliometric evidence of the correlation of organizational changes on the development of its scientific performance; particularly, structural and leadership changes. The study involves six bibliometric indicators to multidimensionally assess the evolution of the institution’s performance profile. For a case study, we selected the Renewable Energy Institute at the National Autonomous University of Mexico, created 35 years ago as a small laboratory, then it evolved to a research center and finally to a formal institute, which over the last 8 years changed from the traditional departmental structure to a network-based structure. The evolution of the multidimensional performance profiles is analyzed, and graphically represented, using a novel artificial intelligence-based approach. We analyzed the performance profiles evolution yearly, using Principal Components Analysis, and a self-organizing neural network mapping technique. This approach, combining bibliometric and machine learning techniques, proved to be effective for the assessment of the institution’s evolution process. The results were represented with a series of graphs and maps that clearly reveal the magnitude and nature of the performance profile evolution, as well as its correlation with each of the structural and leadership transitions. These exploratory results have provided us data and insights into the probable effects of these transitions on academic performance, that have been useful to create a dynamical model.

Integrating dietary supplementation with poppy (Papaver somniferum L.) seed meal: effects on growth performance, nutrient digestibility, and faecal microbiota in broilers

Abstract. The study investigated the effects of supplementing broiler diets with poppy (Papaver somniferum L.) seed meal (PSM) on growth performance, nutrient digestibility, faecal microbiota, and blood profiles. A total of 250 unsexed 1 d old broilers were allocated to five distinct treatment groups. PSM was incorporated into the diets at varying levels: 0 % (PSM0), 5 % (PSM5), 10 % (PSM10), 15 % (PSM15), and 20 % (PSM20). The findings indicated that growth performance, including weight gain, feed intake, and feed conversion ratio (FCR), was significantly improved (P<0.05) in the PSM20 group compared to the control. However, carcass weight experienced a notable decrease (P<0.05) in the PSM20 group. Regarding nutrient digestibility, PSM supplementation led to reduced crude protein digestibility. Nevertheless, apparent metabolizable energy and ash content were significantly enhanced (P<0.05) in the PSM15 and PSM20 groups. Notably, faecal microbiota also experienced substantial improvement (P<0.05) in the PSM20 group. In conclusion, the study demonstrates that incorporating poppy seed meal at a rate of 20 % in broiler diets enhances growth performance, improves nutrient digestibility, and positively influences faecal microbiota composition.

Research Note: Effect of different photoperiodic programs from rearing period on the reproductive performance and hormone secretion of White King pigeons

The photoperiod is an important factor during rearing and laying period that affects age and body weight at sexual maturation and reproductive performance in poultry; however relevant research on this factor in pigeons is still lacking. Thus, this study investigated the effects of different photoperiodic programs on the reproductive performance and hormonal profile in White King pigeons. From 101 d of age, the pigeons in the control group were exposed to a natural photoperiod until 160 d, and then to a photoperiod of 16 h (16 light [L]: 8 dark [D]) and lasted for 200 d. Pigeons in the 3 experimental groups were exposed to a short photoperiod of 8L: 16D until 160 d, and then to 14L: 10D, 16L: 8D, and 18L: 6D, respectively. The results showed that light-restriction (8L: 16D) during the rearing period and then 14L: 10D or 16L: 8D photostimulation delayed the age at first egg laying in pigeons. However, 16L: 8D after an 8L: 16D photoperiod during the breeding period ensured maximum photosensitivity, and significantly improved the reproductive performance (egg production and fertility rates) in pigeons. Moreover, the highest reproductive performance in group under16L: 8D after 8L: 16D photoperiodic program was accompanied by improved follicle-stimulating hormone and estradiol levels and reduced prolactin hormone levels. The results indicated that photoperiodic programs from rearing to laying period are closely related to the reproductive performance of White King pigeons. The results provide information that 8L: 16D during rearing period and 16L: 8D during laying period can be used to enhance reproductive performance in the pigeon industry.

Upper Extremity Musculoskeletal Profiles in Tennis Players: A Systematic Review.

Tennis-specific musculoskeletal (MSK) screening can assess range of motion (ROM) and muscular imbalances. Identifying normative values before implementing a MSK screen is essential in contributing to athlete performance and injury risk profiles. To review upper extremity MSK data in healthy tennis players across age, sex, and level of play. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were followed for this review. A search was conducted in MEDLINE, SPORTDiscus, Embase, and CINAHL. This review included shoulder, elbow, and wrist ROM, isometric strength, or isokinetic strength in a tennis population. Each article was critically appraised to help identify the internal and external validity of each study. Systematic review. Level 3. A total of 41 studies met the search criteria. Each contributor organized the data elements of interest into data tables, with a second contributor assigned for review. Data elements of interest included player and study characteristics: ROM, isometric dynamometry, and isokinetic strength. A total of 3174 players were included in the final studies. Most of the players included were competitive adolescents and young adults; 15 studies included ROM data. Male tennis players consistently had more external rotation (ER) gain (range, 1.8º to 8.8º) and internal rotation (IR) loss (range, -15.3º to -3.0º) when compared with their female counterparts (ER range, -2.5º to 5.8º; IR range, -10.4º to -3º). Shoulder IR and ER strength were measured in the majority of all the strength studies, with the external rotators generating at least two-thirds the strength of the internal rotators. Overall MSK data of tennis players indicate that shoulder strength values are often larger than nontennis players, but equal to or slightly lower than comparable athletes in other overhead sports. Adaptive changes of the glenohumeral joint and subsequent rotational motion are similar to those of other overhead athletes.

Effect of a probiotic blend in broiler chicken diets and its effect on growth performance, carcass traits, and haematological profile

The study aimed to assess the effect of different dietary levels of a probiotic blend (Probio Enzyme®) during the first 14 days of age (DOA) or up to 42 DOA, on growth performance, carcass and digestive tract traits, and haematological profiles of broiler chicks. A total of 540, one-day-old broiler chicks were randomized assigned to nine treatments: four dietary probiotic blend levels (250, 500, 750, and 1,000 g/ton) within two feeding periods (0–14 and 0–42 d, respectively), whereas the ninth treatment was a control diet without any dietary probiotic supplementation. The feed intake (FI) was found to be higher in broilers fed 1,000 g/ton of probiotic blend when fed during the first 14 d; however, BW gain and feed efficiency were not influenced by the treatments. Carcasses (deplumed and full) from broilers fed 250 g/ton of probiotic blend (0–14 DOA) were heavier than the other groups; the same was observed for leg portions. Broiler duodenum, jejunum and ileum weights, and ileum percentage were greater when fed diets without any supplementation. The haematological profile of broilers was not affected by the dietary treatments.

Growth, Hematological Evaluation and Heavy Metal Bioaccumulation in Nile Tilapia (Oreochromis niloticus) from A Municipal Waste Landfill Reservoir

The aim of this study was to evaluate the growth performance, mortality rate, and blood profile of Cd-, Pb-and Cr-contaminated Nile tilapia from the Khon Kaen municipal waste landfill reservoir and compare the parameters with those of fish from unpolluted sites. Human bioaccumulation factor (BAF) determination was also performed. Thirty samples of Nile tilapia were taken from the Khon Kaen municipal waste landfill reservoir (CN) and from unpolluted (TN) ponds. Water and soil were collected from these sites, and tilapia muscle, gill, and liver were obtained for heavy metal evaluation. The Cd concentrations in the muscle, gill and liver were 0.003 ± 0.001, 0.006 ± 0.008, and 0.004 ± 0.003 mg/kg; the Pb concentrations were 0.078 ± 0.173, 0.880 ± 1.360 and 1.110 ± 1.862 mg/kg; and the Cr concentrations were 0.067 ± 0.135, 0.572 ± 1.098 and 0.636 ± 1.272 mg/kg, respectively. The total fish length, body weight and survival rate were significantly lower in the TN group (P <0.05), and alanine aminotransferase and creatinine were significantly (P <0.05) higher in the TN group. The hematocrit count of the TN group was also lower than that of the CN group but without a significant difference. The BAF ranged from 0.1-4.3, especially in the gills and liver, which are major heavy metal accumulation organs. Heavy metal contamination in landfill leachate negatively affects fish health and its production. Keywords: Aquaculture, Nile tilapia, Heavy metal, Pathology, Toxicity

Large‐Area Processable Ultrathin Organic Transistors with High Mobility and Mechanical Stabilities

AbstractFor applications involving the implantation of organic thin‐film transistors (OTFTs) in flexible environments, maintaining uniform device performance and ultrathin profiles with excellent mechanical stability is crucial for dynamic deformable conditions. This study presents high‐performance, mechanically flexible ultrathin OTFTs with a thickness under 5 µm, designed to be scalable for utilization in large areas. The OTFTs utilized an organosilicon polymer dielectric layer synthesized via initiated chemical vapor deposition combined with the crystalline organic semiconductor 2‐Decyl‐7‐phenyl[1]benzothieno[3,2‐b][1]benzothiophene (Ph‐BTBT‐10). Compared with conventional organic transistors, the OTFTs exhibited exceptional features, such as a charge carrier mobility of up to 7 cm2 V−1 s−1 and a near‐zero threshold voltage. Their performance is showcased across 900 devices on a 10 cm × 10 cm substrate to ensure uniformity and scalability. Additionally, the OTFTs demonstrate remarkable resilience to mechanical deformation, with a 97.4% mobility retention rate under a 20% tensile compression. After 1000 cycles of repeated deformation stress, they maintain a robust 94.6% performance level compared with the initial state. In summary, the ultrathin flexible OTFTs exhibit exceptional resilience to mechanical deformation, extended air exposure, uniformity in large arrays, and high mobility. This makes them promising for diverse applications, such as in vivo organ monitoring and skin displays.

Assessment of Full-Fat Tenebrio molitor as Feed Ingredient for Solea senegalensis: Effects on Growth Performance and Lipid Profile.

Tenebrio molitor (TM) is considered as one of the most promising protein sources for replacing fish meal in aquafeeds, among other things because it is rich in protein, a good source of micronutrients and has a low carbon footprint and land use. However, the main drawback of TM is its fatty acid profile, in particular its low content of n-3 PUFA. This study evaluates the effects of partially replacing plant or marine-derived with full-fat TM meal at two different levels on growth performance and lipid profiles of Senegalese sole (Solea senegalensis). For this purpose, a control diet (CTRL) and four experimental isoproteic (53%) and isolipidic (16%) diets were formulated containing 5 and 10% TM meal replacing mostly fish meal (FM5 and FM10), or 10 and 15% TM meal replacing mostly plant meal (PP10 and PP15). Fish (215 g) were fed at 1% of their body weight for 98 days. The final body weight of fish fed the experimental diets containing TM meal was not different from that of fish fed the CTRL diet (289 g). However, the inclusion of TM meal resulted in a gradual improvement in growth rate and feed efficiency in both cases (replacement of fish or plant meals), and significant differences in specific growth rate (SGR) were observed between fish fed the CTRL diet (SGR = 0.30% day-1) and those fed diets with the highest TM meal content (PP15; SGR = 0.35% day-1). The experimental groups did not show any differences in the protein content of the muscle (19.6% w/w). However, significant differences were observed in the total lipid content of the muscle, with the FM10, PP10, and PP15 groups having the lowest muscle lipid contents (2.2% ww). These fish also showed the lowest neutral lipid content in muscle (6.6% dw), but no differences were observed in the total phospholipid content (2.6% dw). Regarding the fatty acid profile, fish fed FM10, PP10 and PP15 had lower levels of linoleic acid (18:2n-6) and higher levels of oleic acid (18:1n-9) in liver and muscle compared to fish fed CTRL. However, no differences were found between fish fed CTRL and TM-based diets for docosahexaenoic acid (22:6n-3) and total n-3 PUFA in liver and muscle. In conclusion, our study demonstrated that full-fat TM inclusion up to 15% in S. senegalensis diets had no negative effects or even some positive effects on fish survival, growth performance, nutrient utilization and flesh quality.

Differential Morpho-Physiological, Ionomic, and Phytohormone Profiles, and Genome-Wide Expression Profiling Involving the Tolerance of Allohexaploid Wheat (Triticum aestivum L.) to Nitrogen Limitation.

Common wheat (Triticum aestivum L.) is a global staple food, while nitrogen (N) limitation severely hinders plant growth, seed yield, and grain quality of wheat. Genetic variations in the responses to low N stresses among allohexaploid wheat (AABBDD, 2n = 6x = 42) genotypes emphasize the complicated regulatory mechanisms underlying low N tolerance and N use efficiency (NUE). In this study, hydroponic culture, inductively coupled plasma mass spectrometry, noninvasive microtest, high-performance liquid chromatography, RNA-seq, and bioinformatics were used to determine the differential growth performance, ionome and phytohormone profiles, and genome-wide expression profiling of wheat plants grown under high N and low N conditions. Transcriptional profiling of NPFs, NRT2s, CLCs, SLACs/SLAHs, AAPs, UPSs, NIAs, and GSs characterized the core members, such as TaNPF6.3-6D, TaNRT2.3-3D, TaNIA1-6B, TaGLN1;2-4B, TaAAP14-5A/5D, and TaUPS2-5A, involved in the efficient transport and assimilation of nitrate and organic N nutrients. The low-N-sensitivity wheat cultivar XM26 showed obvious leaf chlorosis and accumulated higher levels of ABA, JA, and SA than the low-N-tolerant ZM578 under N limitation. The TaMYB59-3D-TaNPF7.3/NRT1.5-6D module-mediated shoot-to-root translocation and leaf remobilization of nitrate was proposed as an important pathway regulating the differential responses between ZM578 and XM26 to low N. This study provides some elite candidate genes for the selection and breeding of wheat germplasms with low N tolerance and high NUE.

Replacing conventional concentrates with sprouted barley or wheat: Effects on lactational performance, nutrient digestibility, and milk fatty acid profile in dairy cows

Finite natural resources, rising human population, and climate change pose challenges to traditional crop production. Hydroponically grown fodder (i.e., sprouted grains) can be an alternative feed source for dairy cows; however, only sprouted barley has been investigated in low-producing cows. We aimed to evaluate the effect of replacing conventional concentrates with sprouted barley or wheat, grown using hydroponics, on milk production, nutrient digestibility, and milk fatty acid profile in high-producing cows. Twenty-four multiparous Holstein cows (3.25 ± 1.33 lactations; 102 ± 23 DIM; 49 ± 4 kg/d of milk) were used in a replicated 3 × 3 Latin square design with 21-d experimental periods. Following a 2-wk covariate period, cows were fed 1 of 3 experimental diets: a TMR (1) without sprouted grains (control), or with (2) 10% sprouted barley, or (3) 10% sprouted wheat on a DM basis. Experimental diets were formulated to be isoenergetic and isonitrogenous with sprouted grains that replaced ground corn, soybean meal, canola meal, and dextrose. Sprouted grains were grown using a semi-automatic hydroponic system and harvested after 6 d of growth. Data and sample collection occurred during the last 3 d of the covariate and experimental periods. Wide ranges were observed for the DM percent of sprouted grains (12.1%-22.9% and 13.3%-25.7% for barley and wheat, respectively) and the ratio of sprouted fodder to seed (0.67-1.07 for both barley and wheat). Feeding sprouted grains did not modify the yield of milk or ECM; however, DMI were lower for barley, relative to control. Feed efficiencies were greater for barley than for control (1.49 ± 0.03 vs. 1.43 ± 0.03 for milk yield/DMI; 1.85 ± 0.03 vs. 1.73 ± 0.04 for ECM/DMI). Yields and concentrations of milk components (i.e., fat, true protein, and lactose) were not affected by treatment. Milk urea N concentrations were greater for wheat, relative to control or barley. Body weight (752 ± 3 vs. 742 ± 3 kg) and BW gains (6.53 ± 2.99 vs. -9.33 ± 2.91 kg/21 d) were higher for wheat than for control. Apparent total-tract digestibility of organic matter was greater for wheat relative to barley. Digestibilities of NDF and starch were higher for wheat and control, relative to barley, and CP digestibility was greater for wheat, relative to barley and control. Rumination and physical activity were not affected by treatment. In summary, replacing traditional concentrates with sprouted grains grown using hydroponics improved milk production efficiency (barley sprouts) or enhanced body weight gain (wheat sprouts). A life-cycle assessment needs to be conducted to determine the net effect of this feeding strategy for the dairy industry.

"Now I Get It!": Eureka Experiences During the Acquisition of Mathematical Concepts.

Many famous scientists have reported anecdotes where a new understanding occurred to them suddenly, in an unexpected flash. Do people generally experience such "Eureka" moments when learning science concepts? And if so, do these episodes truly vehicle sudden insights, or is this impression illusory? To address these questions, we developed a paradigm where participants were taught the mathematical concept of geodesic, which generalizes the common notion of straight line to straight trajectories drawn on curved surfaces. After studying lessons introducing this concept on the sphere, participants (N = 56) were tested on their understanding of geodesics on the sphere and on other surfaces. Our findings indicate that Eureka experiences are common when learning mathematics, with reports by 34 (61%) participants. Moreover, Eureka experiences proved an accurate description of participants' learning, in two respects. First, Eureka experiences were associated with learning and generalization: the participants who reported experiencing Eurekas performed better at identifying counterintuitive geodesics on new surfaces. Second, and in line with the firstperson experience of a sudden insight, our findings suggest that the learning mechanisms responsible for Eureka experiences are inaccessible to reflective introspection. Specifically, reports of Eureka experiences and of participants' confidence in their own understanding were associated with different profiles of performance, indicating that the mechanisms bringing about Eureka experiences and those informing reflective confidence were at least partially dissociated. Learning mathematical concepts thus appears to involve mechanisms that operate unconsciously, except when a key computational step is reached and a sudden insight breaks into consciousness.

Identifying and distinguishing cognitive profiles among virally suppressed people with HIV.

Cognitive deficits are common among people with HIV (PWH), even when virally suppressed. We identified cognitive profiles among virally suppressed PWH and determined how sociodemographic, clinical/behavioral, and HIV disease characteristics distinguish profile membership. Participants included 704 virally suppressed PWH (Mage = 43.9 [SD = 10.2], 88% male, 58.9% non-Hispanic White) from the HIV Neurobehavioral Research Program. Demographically adjusted T scores were derived from a neuropsychological evaluation comprised of 13 tests. We implemented a pipeline involving dimension reduction and clustering to identify profiles of cognitive performance. Random forest models on a 70/30 training/testing set with internal cross-validation were used to identify sociodemographic, clinical/behavioral, and HIV disease correlates of profile membership. Six cognitive profiles were identified: (a) "unimpaired" (19.9%); (b) weakness in verbal learning and memory (15.5%); (c) weakness in executive function and learning (25.8%); (d) weakness in motor, processing speed, and executive function (8.1%); (e) impaired learning and recall with weak-to-impaired motor, processing speed, and executive function (13.1%); (f) global deficits (17.6%). The most discriminative sociodemographic, clinical/behavioral, and HIV disease characteristics varied by profile with self-reported mood symptoms and cognitive/functional difficulties (e.g., language/communication, memory, and overall everyday function complaints) most consistently associated with profile membership. Cognitive profiles and their associated factors among PWH are heterogeneous, but learning/memory deficits were most common and self-reported mood, and cognitive/functional difficulties were most consistently related to profile membership. This heterogeneity in cognitive profiles and their correlates in PWH suggests that differing mechanisms contribute to cognitive deficits and, thus, underscores the need for personalized risk reduction and therapeutic strategies among PWH. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Exploring metabolic responses and pathway changes in CHO-K1 cells under varied aeration conditions and copper supplementations using 1 H NMR-based metabolomics.

The optimization of bioprocess for CHO cell culture involves careful consideration of factors such as nutrient consumption, metabolic byproduct accumulation, cell growth, and monoclonal antibody (mAb) production. Valuable insights can be obtained by understanding cellular physiology to ensure robust and efficient bioprocess. This study aims to improve our understanding of the CHO-K1 cell metabolism using 1 H NMR-based metabolomics. Initially, the variations in culture performance and metabolic profiles under varied aeration conditions and copper supplementations were thoroughly examined. Furthermore, a comprehensive metabolic pathway analysis was performed to assess the impact of these conditions on the implicated pathways. The results revealed substantial alterations in the pyruvate metabolism, histidine metabolism, as well as phenylalanine, tyrosine and tryptophan biosynthesis, which were especially evident in cultures subjected to copper deficiency conditions. Conclusively, significant metabolites governing cell growth and mAb titer were identified through orthogonal partial least square-discriminant analysis (OPLS-DA). Metabolites, including glycerol, alanine, formate, glutamate, phenylalanine, and valine, exhibited strong associations with distinct cell growth phases. Additionally, glycerol, acetate, lactate, formate, glycine, histidine, and aspartate emerged as metabolites influencing cell productivity. This study demonstrates the potential of employing 1 H NMR-based metabolomics technology in bioprocess research. It provides valuable guidance for feed medium development, feeding strategy design, bioprocess parameter adjustments, and ultimately the enhancement of cell proliferation and mAb yield.

Long-term tadalafil once daily in Chinese men with erectile dysfunction: a 2-year final analysis of a post-marketing, multicenter, randomized, open-label trial.

The long-term safety and effectiveness of once-daily tadalafil is crucial, but limited data are available in Chinese patients with erectile dysfunction (ED). In this post-marketing, multicenter, randomized, open-label trial with 2-year follow-up, 635 ED cases were randomized to receive daily oral tadalafil 2.5 mg or 5 mg for 3 months, of whom 580 continued once-daily tadalafil 5 mg for 21 months. Treatment-emergent adverse events in the 12-month and 24-month period were similar, with the most common being viral upper respiratory tract infection, upper respiratory tract infection, and headache. Significant improvement from baseline in the International Index of Erectile Function-Erectile Function (IIEF-EF) score was detected at month 12 (least squares mean [LSM] change: 7.9, 95% confidence interval [CI]: 7.5-8.4, P < 0.001) and was maintained to month 24 (LSM change: 8.6, 95% CI: 8.1-9.0, P < 0.001). The proportions of patients regaining normal erectile function (IIEF-EF score ≥26) were 43.7% and 48.0% at months 12 and 24, respectively. Global Assessment Questionnaire results showed improved erection function in 97.5% of patients and improved ability to engage in sexual activity in 95.9% of patients at month 12; these values were 96.1% and 95.0% at month 24, respectively. The quality of sexual life score based on the Sexual Life Quality Questionnaire (SLQQ) was increased by 52.2% at month 12 and by 55.3% at month 24 (both P < 0.001). The treatment satisfaction score determined by SLQQ (mean ± standard deviation) was 62.4 ± 21.0 at month 12 versus 65.9 ± 20.2 at month 24. Two-year daily application of tadalafil 5 mg in Chinese men with ED showed a favorable safety profile and durable improvement in sexual performance and satisfaction.