ABSTRACT Perchlorate () industrial wastewater requires efficient removal to prevent adverse environmental impacts, however, high concentration and low biodegradability give rise to poor bioreduction performance. -autotrophic granular sludge (-AuGS) was firstly cultivated for high concentration perchlorate () removal in the upflow anaerobic sludge blanket (UASB) reactor (: 150 mg L−1). Simultaneously, the was utilized to control the generation as electron donor, the effluent concentration (190 mg L−1) was satisfied with drinking water standard (250 mg L−1). Under the optimized condition of hydraulic retention time (HRT) (6 h) and / molar ratio (2.2), more EPS was secreted, which promoted the -AuGS formation and stability. Though acclimation of 146 d, the -AuGS was formed with a large average granular sludge size (612 μm) and an excellent settleability (sludge volume index: SVI5/SVI30 = 1). With the mature -AuGS formation, the highest and loading was increased to 1.06 and 0.75 kg m−3 d−1. Interestingly, Georgfuchsia, Methyloversatilis, Sulfurisoma, and Exiguobacterium were the main microbial community in the -AuGS. This study proposed to form a novel -AuGS for developing the high concentration removal performance and to utilize the as an electron donor for controlling the excessive generation.