Percentage Of Total Lipids Research Articles

Lipid profiling in sewage sludge

High value-added reutilization of sewage sludge from wastewater treatment plants (WWTPs) is essential in sustainable development in WWTPs. However, despite the advantage of high value reutilization, this process must be based on a detailed study of organics in sludge. We used the methods employed in life sciences to determine the profile of lipids (cellular lipids, free fatty acids (FFAs), and wax/gum) in five sludge samples obtained from three typical WWTPs in Beijing; these samples include one sludge sample from a primary sedimentation tank, two activated sludge samples from two Anaerobic-Anoxic-Oxic (A2/O) tanks, and two activated sludge samples from two membrane bioreactor tanks. The percentage of total raw lipids varied from 2.90% to 12.3%. Sludge from the primary sedimentation tank showed the highest concentrations of lipid, FFA, and wax/gum and the second highest concentration of cellular lipids. All activated sludge contained an abundance of cellular lipids (>54%). Cells in sludge can from plants, animals, microbes and so on in wastewater. Approximately 14 species of cellular lipids were identified, including considerable high value-potential ceramide (9567–38774 mg/kg), coenzyme (937–3897 mg/kg), and some phosphatidylcholine (75–548 mg/kg). The presence of those lipid constituents would thus require a wider range of recovery methods for sludge. Both cellular lipids and FFAs contain an abundance of C16–C18 lipids at high saturation level, and they serve as good resources for biodiesel production.

Fatty acid composition of five Algerian bisexual and parthenogenetic strains of Artemia (Anostraca, Crustacea) and their antimicrobial activity

The brine shrimp Artemia remains among the most important food used in fish and crustacean larviculture, and the study of its cysts’ nutritional quality is of great importance for products selection. Fatty acids (FAs) from decapsulated cysts of two bisexual (A. salina) and three parthenogenetic strains (A. parthenogenetica) from Algeria have been analyzed by gas chromatography. The aim was, first, to check the suitability of these strains as food for aquaculture and, second, to evaluate the FAs’ efficiency in inhibition of some of the fish pathogen’s activities. Percentages of total lipids were between 7.78 and 24.55% for the parthenogenetic strains of Bethioua and Sidi Bouziane salterns, respectively. Twenty-three FAs have been detected, and a high value of eicosapentaenoic (EPA; 20:5n-3) was observed in the parthenogenetic strain of Sidi Bouziane with 16.25 ± 0.08% (39.89 mg g−1 dry weight). The highest value of α-linolenic (ALA; 18:3n-3) was observed in A. salina from Chott Marouane with 22.28 ± 0.16%, (30.72 mg g−1 dry weight). The results of the antimicrobial screening assay of FA extracts showed their inhibitory activity against six fish pathogens. In comparison with ampicillin (Amp) and kanamycin antibiotics, all the studied strains of Artemia, except Bethioua, had better antimicrobial effect of their FAs against Vibrio anguillarum (gram-negative) than Amp. Streptococcus agalactiae (gram-positive) was the most resistant against Artemia’s FAs. Decapsulated cysts with the highest proportions of PUFAs and rich in ALA and linoleic were the most effective against the bacterial growth. Regarding the FAs’ composition and their antibacterial properties, Algerian strains of Artemia are good candidates for use as food in larviculture.

Lake whitefish ( Coregonus clupeaformis ) energy and nutrient partitioning in lakes Michigan, Erie and Superior

A concurrent decrease in lake whitefish (Coregonus clupeaformis) condition and Diporeia spp. abundance in Lake Michigan has spurred investigations into possible links between the two phenomena. We examined female lake whitefish δ13C and δ15N stable isotopes, growth, reproductive investment, dorsal muscle total lipid and docosahexaenoic acid (DHA) contents from lakes Erie, Michigan and Superior to determine whether differences in food source were correlated with measures of stock success. Stocks with higher somatic growth rates and mean reproductive potential had higher energy stores in terms of percent total lipid. Stocks with low muscle lipid concentration also had smaller egg sizes as egg number increased. Diet varied among stocks as evidenced by δ13C and δ15N stable isotope analyses; however, muscle total lipid and DHA were not correlated to apparent Diporeia spp. prey use. When compared to stocks from lakes Erie and Superior, Lake Michigan stocks had lower growth, reproduction, and lipid stores. While stocks in Lake Michigan with access to declining Diporeia spp. populations may still feed on the amphipod, it appears that they are unable to consume the quantities necessary to maintain historical growth and reproduction. Stable isotope analyses of lakes Erie and Superior stocks, with higher growth rates and lipid values, indicated different feeding strategies with no indication of reliance on Diporeia spp. While differences in prey resources may have an effect on lake whitefish stocks, differences in Diporeia spp. abundance alone cannot explain differences in lake whitefish condition observed among the Great Lakes included in this study.

Preservation of anthocyanins in solid lipid nanoparticles: Optimization of a microemulsion dilution method using the Placket–Burman and Box–Behnken designs

Anthocyanins are the main polyphenol components from red cabbage (Brassica oleracea L. Var. Capitata f. Rubra) extracts that have inherent antioxidant activities. Anthocyanins are effectively stable in acidic gastric digestion conditions, with nearly 100% phenol content recovery. However, the total phenol content recovery after simulated pancreatic digestion was approximately 25%. To protect anthocyanins against harsh environmental conditions (e.g., pH and temperature), solid lipid nanoparticles were prepared by the dilution of water in oil (w/o) microemulsions containing anthocyanins in aqueous media. The formulations were characterized for particle size and encapsulation efficiency. The formulation parameters (e.g., volume of the internal aqueous phase, homogenization time and the percentages of total lipid, total surfactant or stabilizer) were optimized using the Placket–Burman and Box–Behnken experimental designs. Entrapment efficiency (89.2±0.3%) was calculated when the mean particle size was 455±2nm. A scanning electron microscopy study revealed the spherical morphology of the particles.

Observation on the Biochemical Constituents of Threadfin Bream Nemipterus japonicus during Gonad Maturation from Suez Gulf, Red Sea, Egypt

The present study revealed that the biochemical constituent with respect to different maturity stages of Nemipterus japonicus fish is associated with reproductive cycle, storage and utilization of reserves. Gonadosomatic index N. Japonicus recorded high values (3.5 and 0.51) in May and low values (0.4 and 0.25) in February for female and male, respectively. The maturity stages was classified into 5 maturity stages as follow, 1) immature stage, 2) maturing stage, 3) mature stage, 4) ripe stage and 5) spent stage. The spawning season of N. japonicus extend from May to September. The biochemical composition in muscle, liver and gonad in both sexes are found higher in early stages of maturity and decreases during gonad maturation. The muscle had more fat and crude protein content during the immature stage. Early stages of maturation, hence offer greater food value in these species. With the advancement of maturity, a drop in the fat and soluble crude protein was observed, whereas a linear relationship with moisture was noted from immature to mature stage. The composition of muscle always demonstrated an inverse relationship with hepatic and gonadal composition. Highest crude protein percentage was in stage IV (72.0 ± 2.22) in ovary. The total crude lipid percentage of the ovary showed a gradual increase from Stage I (5.1 ± 0.47) to Stage IV (21.8 ±1.02).

The effect of supplementing rumen undegradable unsaturated fatty acids on marbling in early-weaned steers.

The objective of this study was to determine if supplementation with a rumen undegradable unsaturated fatty acids (FA) source improved marbling deposition in early-weaned steers. All steers (Angus, = 23; Angus × Hereford, = 24) were weaned at 150 ± 5 d of age. Steers were blocked by BW and breed and then randomly assigned to either control (CON; average 1.5 kg of corn gluten feed [CGF], = 23) or isocaloric supplementation containing a rumen undegradable fat source (RUF; 200 g of Megalac-R added to an average of 1.06 kg of CGF, = 24) offered 5 d/wk for 110 d. All steers had ad libitum access to pastures throughout treatment and received supplements individually. Steer BW and blood samples were collected at 0, 55, and 110 d of supplementation, and real-time ultrasound measurements were collected at d 110. Following treatment, steers were transported to Oklahoma State University for finishing and subsequent harvesting at a commercial plant. All data were analyzed using the PROC MIXED procedure of SAS either as repeated measures or ANOVA depending on parameters. There were no significant changes in BW from the beginning of treatment to harvest due to treatment. Ultrasound data showed that RUF steers tended ( = 0.08) to have more i.m. fat than CON at d 110. Serum concentrations of FA showed a treatment × day interaction ( < 0.02) for 16:0, 18:0, 18:1-9, 18:2, 20:4, and total FA. These specific FA concentrations slightly increased in CON steers, but there was a more pronounced increase in the concentration of these FA across the supplementation period in RUF steers. Serum triglyceride and cholesterol concentrations were increased ( < 0.01) on d 55 and 110 in RUF steers compared with those in CON steers. Serum leptin concentration in RUF steers was greater ( < 0.01) than CON steers at d 110. After harvest, RUF carcasses had greater ( = 0.01) marbling scores than those of CON carcasses. All other carcass measures were similar between treatments. The percentage of total lipids was increased ( = 0.011) in steaks from RUF compared to CON. There was a decrease ( < 0.05) in adipocyte diameter in i.m. fat depot of RUF steers compared to CON. There was also a tendency ( = 0.06) for RUF steers to have a greater percentage of 20 to 30 μm adipocytes in their i.m. depot than CON steers. This study indicates that supplementation of unsaturated FA can positively impact marbling deposition in early-weaned steers without impacting other carcass measures.

Effects of different vaccine combinations against Mycoplasma gallisepticum on the internal egg and eggshell characteristics of commercial layer chickens , ,

Live F-strain Mycoplasma gallisepticum (FMG) vaccines are presently being used to help control field-strain MG outbreaks. However, they may exert some adverse effects on egg production. Live strains of MG of lesser virulence as well as killed vaccines have little or no effect on egg production, but afford lower levels of protection. This has led to research investigating their use in combination with a subsequent overlay vaccination of FMG given later in the production cycle. In the present study, 2 trials were conducted to investigate the effects of prelay vaccinations of live and killed MG vaccines or their combination, in conjunction with an FMG vaccine overlay after peak production, on the egg characteristics of commercial layers. The following vaccination treatments were administered at 10 wk of age (woa): 1) unvaccinated (Control), 2) MG–Bacterin (MGBac) vaccine, 3) ts-11 strain MG (ts11MG) vaccine, and 4) MGBac and ts11MG combination (MGBac + ts11MG). At 45 woa, half of the birds were overlaid with an FMG vaccine. In each trial, internal egg and eggshell parameters including egg weight (EW), Haugh unit score (HU), eggshell breaking strength (EBS), percentage yolk weight (PYW), percentage albumen weight (PAW), percentage eggshell weight (PSW), eggshell weight per unit surface area (SWUSA), percentage yolk moisture (PYM), and percent total lipids (PYL) were determined at various time periods between 21 and 52 woa. At 28 woa, SWUSA was lower in the ts11MG and MGBac + ts11MG groups compared to the Control group. Conversely, at 43 woa, SWUSA was higher in the ts11MG than in the MGBac group. Between 23 and 43 woa, PYL was higher in the MGBac and ts11MG groups in comparison to the Control group. In conclusion, vaccination with MGBac alone or in combination with ts11MG at 10 woa with or without an FMG vaccine overlay at 45 woa does not adversely affect the internal egg or eggshell quality of commercial layers throughout lay.

Detection of Interactions of Inositol Phospholipids with Ion Channels

Inositol phospholipids account for only a small percentage of total lipids in cell membranes. But they play crucial roles in diverse cellular processes such as signal transduction, cytoskeleton organization, membrane trafficking and membrane permeability and transport. While some of the functions involve regulated hydrolysis to generate second messengers, others are thought to involve direct interactions with proteins. The repertoire of phosphoinositide-binding proteins has been dramatically expanded in recent years. These include many ion channels, from the first identified prototype inward rectifier potassium channel (Kir) to the more recent transient receptor potential channels. However, in most cases the roles of the lipids are implicated from pharmacological maneuvering of signaling pathways. A direct observation of the interaction between lipids and proteins has rarely been possible. The recognition sites on the proteins are often interfered by structural-functional studies using site-directed mutagenesis. Owing to the complexity of gating mechanisms, the molecule sites identified in this manner have been less reliable. In this work we present a FRET approach for direct detection of interactions between phosphoinositide lipids and ion channel proteins. Using an inward rectifier potassium channel and a transient receptor potential channel as prototype models, we show the feasibility to generate recombinant proteins incorporating genetic donors. We use fluorescent lipids as acceptors and show that the energy transfer is limited to only peripheral lipids around channel proteins. Our experiments demonstrate that the assay has a resolution to probe specifically bound lipid molecules.

Análisis cuantitativo de la ingesta de nutrientes en niños menores de 3 años. Estudio ALSALMA

ObjectiveThe objective of the study was to analyze the nutritional patterns of children under three years of age and to compare the results against the recommendations for energy and nutrient intake. Patients and methodsIn this cross-sectional epidemiological study, parents completed a dietary diary on their food intake of their children on 4 non-consecutive days. The percentage of children with mean intakes below the recommendations for each age and nutrient was analyzed using the “Estimated Average Requirement (EAR) cut-point method.” ResultsA total of 186 pediatricians included 1701 children in the study. A total of 95.9% (n=1320) of the children between 7 and 36 months had a protein consumption more than twice that of the Recommended Daily Allowances. The deficiencies observed (% < EAR) in the age groups 13-24 months and 25-36 months, respectively, were: vitamin D in 81.7% and 92.1%; vitamin E in 39.3% and 53.4%; folic acid in 12.5% and 14.8%; calcium in 10.1% and 5.5%; iodine in 27.1% and 31%. It was observed that a higher percentage in the daily intake of proteins (P=.013) and of carbohydrates (P<.0001), and a lower percentage of total lipids (P<.0001), were related to a greater body mass index, regardless of energy intake. ConclusionsThe study presents a very detailed view of the eating patterns of Spanish children less than three years of age. The encouragement of healthy feeding should be directed towards the correction of the dietary imbalances detected, in order to promote the future health of children.

Physiological and hormonal changes during prolonged starvation in fish

Starvation periods are common for many animals, including fish, birds, and mammals. Many fish species exhibit extraordinary resilience to prolonged starvation, but the reasons for that and the mechanisms employed during these periods are poorly understood. This study shows that similar to mammals and birds, many fish species undergo three phases during starvation: (I) a short transient phase, (II) a long, protein conservation steady state phase with mainly fat oxidation as the primary energy source, and (III) a shift to protein mobilization as a main energy source. These starvation states and their transitions were quantified by a meta-analysis of large empirical data available in the literature, revealing that low critical levels of fat reserves trigger the transition to the third state. The critical fat level, denoted as γ (percentage of total lipid in body mass), ranges between 0.7% and 5%, depending on the species. It appears that these transitions in the energy mobilization phases are regulated by hormonal changes, including growth hormone (GH), leptin, cortisol, and ghrelin, but the exact mechanisms are still unclear and should be further investigated. Simulations in silico of starvation periods at various temperatures using a dynamic model indicated that temperature affects the length of Phase II because of changes in protein metabolism, with consequences on the ability to withstand prolonged starvation.

Effect of phosphate on lipid metabolism in Fusarium oxysporum

The effect of different phosphate levels in the growth medium on lipid metabolism in &lt;i&gt;Fusarium oxysporum&lt;/i&gt;, was studied. An increase in the phosphate level led on to an increase in the phosphorus content of the mycelium, as well as in that of total lipids. However, higher phosphate concentrations, had no profound effect on the total lipid percentage. Among phospholipid compounds phosphatidic acid (PA) and phosphatidyl inositol were the most susceptible while phisphatidyl choline (PC) was found to be the least affected by changes in the phosphate content of the medium. In the pulse and chase experiments, higher specific activities were observed for PA, PGP and cardiolipin (CL) fractions.

The bioenergetic fuel for non-feeding larval development in an endemic palaemonid shrimp from the Iberian Peninsula, Palaemonetes zariquieyi

Palaemonetes zariquieyi, an endemic palaemonid species of shrimp that lives in freshwater and brackish coastal habitats in eastern Spain, shows an abbreviated, non-feeding larval development comprising only three zoeal stages. To identify the endogenous bioenergetic fuel that allows for food-independent development from hatching to metamorphosis, larvae were reared under controlled laboratory conditions, and ontogenetic changes in dry weight (W), elemental (CHN), and lipid composition (total lipids, principal lipid classes, and fatty acids [FA]) were quantified at the onset of each zoeal stage and in the first juvenile. Values of W, C, and H per larva and per mass unit of W decreased throughout the time of larval development, while the N content showed only a weak decline (suggesting strong lipid but only little protein degradation). Correspondingly, directly measured values of total lipids (both in μg/larva and in % of W) decreased gradually, with neutral lipids (NL) consistently remaining the predominant and most strongly used fraction; sterol esters and waxes were not detected. In contrast to the NL, the fraction of polar lipids (PL) per larva remained stable and, as a consequence, tended to increase as a percentage of total lipids. Likewise, other important lipid fractions such as free FA and cholesterol remained stable throughout the time of larval development. Among the FA, palmitic (16:0), oleic (18:1n–9), linoleic (18:2n–6), and eicosapentaenoic (20:5n–3) acid were predominant, showing a significant decrease during larval development; stearic (18:0), vaccenic (18:1n–7), and arachidonic acid (20:4n–6) were found only in small amounts. Our results indicate that the lecithotrophic development of P. zariquieyi is primarily fuelled by the utilization of lipids (especially triacylglycerides and other NL), which is reflected by a decreasing carbon content. Proteins and PL, by contrast, are preserved as structurally indispensable components (nerve and muscle tissues, cell membranes). The abbreviated and non-feeding mode of larval development of P. zariquieyi may have an adaptive value in land-locked freshwater habitats, where planktonic food limitation is likely to occur. The patterns of reserve utilization are similar to those previously observed in other palaemonid shrimps and various other groups of decapod crustaceans with lecithotrophic larvae. This suggests a multiple convergent evolution of bioenergetic traits allowing for reproduction in food-limited aquatic environments.

Effects of microalgal diet on growth, survival, biochemical and fatty acid composition of Ruditapes decussatus larvae

Successful bivalve larval growth and survival depend on stored reserves provided by diet. The aim of this study was to evaluate the effects of microalgal diet on growth, survival, together with biochemical and fatty acid composition during larval development in grooved carpet shell (Ruditapes decussatus). Four mono- and multi-species diets were tested: (1) Isochrysis galbana, Pavlova lutheri and Chaetoceros muelleri (1:1:1, IPC); (2) I. galbana, P. lutheri and C. muelleri (1:1:2, IP2C); (3) I. galbana and P. lutheri (1:1, IP); (4) C. muelleri (C). Our results showed that feeding regime greatly influenced larval growth and survival, as well as biochemical and fatty acid composition. Higher growth and survival rates were observed in the IPC, IP2C and C diets, which all include C. muelleri. Despite the fact that the larvae fed with IP exhibited the lowest length at settlement and survival, they stored more total lipids than any of the diets including C. muelleri. The IP and IPC diets exhibited a higher percentage of total lipids, whereas IP2C and C showed a higher proportion of proteins. Lipid and carbohydrate concentrations in these diets negatively affected growth and growth and survival of the larvae, respectively. Protein content of the larvae was however positively related to larval survival, whereas lipid content of the larvae negatively affected growth. Fatty acid (FA) profile of neutral lipids (NL) and polar lipids (PL) of 22-day-old larvae clearly reflected the FA proportion of each diet. Dietary DHA and DHA in NL of the larvae were negatively related to larvae growth. DHA incorporation into PL in larvae fed with DHA-deficient diets was compensated by an enrichment in EPA, 18:0dma, 22:5(n-3) and 22:2 non-methylene-interrupted dienoic FA. According to these results, the presence of C. muelleri in the diet seems to promote better growth and survival of R. decussatus larvae.

Phytochemical analysis of Convolvulus hystrix Vahl and its biological effects in rats

"Convolvulus hystrix Vahl" is a plant that has been known to Ancient Egyptians and to Arabs and its root was used traditionally as a purgative. Our attention was directed to the root bark as we observed that the largest part of the plant is deeply impeded underground. The work plan involved testing experimental animals for the influence of aqueous root bark extract on carbohydrate, fat and protein metabolisms as reflected on the growth and relevant laboratory metabolic assessment parameters. Proximate analysis showed high percentages of moisture (85%) and total lipids (7.2%) and surprising amounts of elements such as barium, strontium, rhodium and tellurium (1.7mg, 3.1mg, 8mg and 9.1mg/g ash, respectively). Random serum glucose value showed a significant decrease in the treated female group (p<0.05). Serum total proteins of treated female group were found to be increased significantly (p<0.001), while it was found to be decreased in the relevant treated male group (p<0.01), together with a significant decrease in blood urea nitrogen (p<0.05), with a significant increase in the serum creatinine (p<0.05). Concerning serum fat metabolic parameters, a significant decrease in the serum triglycerides and high-density lipoproteins (p<0.01 and p<0.01, respectively) were found. We concluded that the presence of huge amount of polyphenolics such as tannins is responsible for the overall results documented as growth retarding and antinutritional factors. The results were motivating and pointed out to the possible opening of vast areas of research in the field of natural products. We recommend a series of biochemical and pharmacological studies concerning different parts of the plant as well.

Carcass analog addition enhances juvenile Atlantic salmon (Salmo salar) growth and condition

Our study used historic marine-derived nutrient (MDN) delivery timing to simulate potential effects of restored connectivity on juvenile Atlantic salmon (ATS; Salmo salar) growth and condition. Four headwater streams were stocked with ATS young of the year (YOY) and received carcass analog additions (0.10 kg·m–2wetted area) in treatment reaches to match the timing of sea lamprey (Petromyzon marinus) spawning. Individual ATS mass was 33%–48% greater and standard length was 9%–15% greater in treatment reaches relative to control reaches for 4 months following nutrient additions. Percent total lipids in YOY ATS were twice as great in treatment reaches 1 month following carcass analog additions and remained elevated in treatment fish for 2 more months. Absolute growth rates, based on otolith microstructure analysis, correlated with water temperature fluctuations in all reaches and were elevated by an average of 0.07 mm·day–1in treatment reaches for 1 month following carcass analog additions. Simulated sea lamprey MDNs increased juvenile ATS growth, which, via potential increases in overwinter survival and decreases in smolt age, may contribute to population persistence and ecosystem productivity.

Development of Biscuit Type Cookie with Partial Replacement of Fat by Inulin

Cookies are bakery products rich in fat and sugars. Lipids have in general important nutritional and technology factors, but excessive intake can bring health risks. The reduced caloric value and lipid content is a common demand among biscuits consumers, which come to own up to 60% fat. This study aimed at developing formulations of cookies with partial replacement (Standard-0%, R1-25%, R2-50% and R3-75%) of fat by inulin as well as evaluating physical, physicochemical and sensory characteristics. Through the present study, it was observed that the partial replacement of fat by inulin in the production of cookies was effective in reducing the percentage of total lipids in the final product, with maximum reduction of 86% in formulation R3, without changing the parameters for moisture, crude fiber and instrumental color. Once insulin was increased, the size of the cookies after baking decreased, therefore increasing the crispness of the cookies made from the formulation with the highest level of replacement, represented by R3, which negatively impacted the sensory evaluation of flavor and purchase intention for the product, while formulation R2, with 50% fat replacement by inulin, presented similar results to the standard product, being the formulation with the greatest sensory acceptance and physical characteristics. Therefore formulation R2 was been good in nutritional and sensorial performance, and then it is best formulation.

Insulin effect on lipogenesis and fat distribution in three genotypes of ducks during overfeeding

In waterfowl, the response to overfeeding differs from one genotype to the other. Pekin ducks generally store lipids in the peripheral tissues while Muscovy and mule ducks promote hepatic lipid storage. A possible reason for these various susceptibilities to hepatic steatosis could be a difference in insulin sensitivity. We suggest a resistance to insulin in Pekin ducks. In the present work we investigate the action of insulin on glucose and lipid metabolisms for the three overfed genotypes. Regardless of the kind of genotype, all ducks appear to be sensitive to insulin: their glycemia is lower when the animals are treated with insulin. Insulin-treated Muscovy and Pekin ducks present a lower increase in total body weight (−16.5% for Muscovy; −8.3% for Pekin); and a significantly lower liver weight than the controls (−9.6% and −18.3%). The percentage of total lipids in the liver is higher in the controls than in the insulin-treated Pekin and mule ducks (respectively −40.4% and −34.7%), which means a decreased hepatic lipogenesis. Pekin ducks present a higher pectoral muscle weight when the individuals are insulin-treated (+9.7%). Lipoprotein lipase (LPL) activity appears to be significantly higher in insulin-treated Pekin and Muscovy ducks (1.39 and 3.38 times greater than controls). Insulin-treated mule ducks present a decrease of muscle and abdominal lipid storage compared to controls (−11.6% and −13.8%). In this experiment, exogenous insulin has induced an increase of lipid oxidation and has led to a less favorable use and storage of dietary glucose. The hypothesis of insulin-resistance of Pekin ducks is not verified.

Cultivating Microalgae in Domestic Wastewater for Biodiesel Production

The objective of this study was to evaluate the growth of nine species of microalgae (green and blue green microalgae) on domestic wastewater obtained from Zenein Wastewater Treatment Plant (ZWWTP), Giza governorate, Egypt. The species were cultivated in different wastewater treatments namely: without treatment; after sterilization; with nutrients with sterilization and with nutrients without sterilization. The experiment was conducted in triplicate and cultures were incubated at 25±1°C under continuous shaking (150 rpm) and illumination (2000 Lux) for 15 days. Algal growth parameters i.e., pH, electric conductivity (EC), optical density (OD), dry weight (DW) and chlorophyll-a (Ch-a) were measured at zero-time and at the end of the experimental period; while, the percentages of total lipids, biodiesel and the residual sediments (glycerine, pigments, etc) were determined in the harvested algal biomass. The data revealed that domestic wastewater with nutrients and with sterilization (T 3 ) was promising for cultivating five algal species as compared to the synthetic media. Moreover, the sterilized-domestic wastewater (T 2 ) was the selective medium for cultivating Oscillatoria sp. and Phormedium sp; however, T 1 medium (wastewater without treatment) was the promising medium for cultivating Nostoc humifusum. Biodiesel production from the algal species cultivated in synthetic media was ranging between 3.90% (Wollea saccata) and 12.52% (Nostoc muscorum). On the other hand, the highest biodiesel production from algal biomass cultivated in wastewater was obtained by Nostoc humifusum (11.80%) when cultivated in wastewater without treatment (T 1 ) and the lowest (3.80%) was recorded by Oscillatoria sp. when cultivated on the sterilized-domestic wastewater (T 2 ). The results of this study suggest that cultivating microalgae on domestic wastewater combines nutrients removal and algal lipid production for potential use as a biodiesel feedstock. Additionally, using the domestic wastewater, as nutrient media for microalgae cultivation, is suitable and non-expensive method as compared to the conventional cultivation methods for sustainable biodiesel and glycerol production.

Significance of lipid binding on the functional and nutritional profiles of single and multigrain matrices

Lipid fractions and starch- and protein-lipid binding of single and blended oat, rye, buckwheat and wheat flour, dough and bread matrices were investigated, and results correlated with the functional and nutritional properties of the grain matrices during mixing and baking. Non-starch lipid was the most prominent fraction in terms of absolute content and as a percentage of total lipids. Free lipids, starch lipids and bound lipids were, respectively, the major, intermediate and minor lipid fractions in flours, doughs and breads. Great differences in total lipid content due to sampling result in divergences amongst lipid fraction content and distribution, especially for starch and bound lipid fractions. Lipids bound to proteins during dough mixing are translocated and bound to starch during baking. In blended samples, the higher fibre content seems to provoke a reduction of the lipid–protein and lipid–starch linkages due to interactions between fibres and endogenous biopolymers. Starch lipid showed the most significant correlations with parameters related to dough and bread performance during breadmaking, especially over the mixing step. Valuable fresh bread functional characteristics, such as high specific volume and high sensory score for softness and overall acceptability, correspond to a starch lipid’s increase due to mixing. The higher the free and starch lipids decrease by reason of temperature treatment—baking—the larger the starch hydrolysis and the higher β-glucans and total dietary fibre contents.

EFFECT OF FEEDING WITH MIXTURE OF COLOURD RICE ON SERUM LIPID PROFILE AND ANTIOXIDANT STATUS IN EXPERIMENTAL RATS

The present investigation was carried out to study the influence effect of colourd rice mixture on serum lipid parameters and hepatic enzyme activities in rats and effect of protein, total lipids, crude fiber and ash of rice samples.The data pointed to the following results: Milling processes led to decrease the percentage of protein, total lipids, crude fiber and ash of rice samples. Whereas it was increased the carbohydrate content in white rice samples. The total phenolic compound content (TPC) of Brown rice varieties had the highest value (1.6 and1.1 mg GAE/g) of Black and Giza 178, respectively, whilst milled rice varieties had the lowest ones (0,8 and 0.5 mg GAE/g). Antioxidant activity was determined in brown black, white black, brown and white Giza 178 extracts. The present study cleared that brown black extract had the highest the antioxidant activity among the investigated extracts followed by brown Giza 178, white black and white Giza 178 extracts. There are nine phenolic acids where identified. Ferulic acid was the major phenolic compound in all rice samples. Furthermore, the highest concentration of ferulic acid that found in brown black rice (4.82 mg/100g). Results of the biological experiment revealed that, the final body weight and weight gain of rats fed on mixture of brown rice and black rice were significantly lower, but food intake was higher compared with the rats fed on basal diet at the end of the experimental period (8 weeks), As for total Cholesterol (T.C), Low density liboprotien cholesterol (LDL-C) and Plasma Triglyceride (T.G) of rats fed on white rice dite were higher than other groups. Furthermore, HDL-C significantly higher in rats fed on mixture of (white rice + black rice) and (brown rice + black rice) diets compared to those fed on white rice and mixture of white rice and brown rice ones. The antioxidant levels in the rat livers ranged from highest in those fed on ( brown rice + black rice) diet, followed by white rice + black rice then white rice + brown rice . Whilst, rice had the lowest level . Finally, it can be concluded that, using brown and black rice have the pronounced effect for lowering cholesterol levels of the blood in experimental rats.