Percentage Of Acids Research Articles

High-Fat Diet Enhances Platelet Activation and Is Associated with Proprotein Convertase Subtilisin Kexin 9: An Animal Study.

Platelet activation and proprotein convertase subtilisin kexin 9 (PCSK9) play pivotal roles in the progression of atherosclerosis to cardiovascular events. It has been reported that hyperlipidemia, a well-documented risk factors for cardiovascular diseases, tends increase platelet activation and PCSK9 expression. However, little is known about this specific mechanism, particularly how nutrition affects platelet activation and PCSK9 levels in hyperlipidemia conditions. This study aimed to assess how a high-fat diet influences platelet activation, its association with PCSK9, and the effects on blood pressure in an animal model. Here, male Wistar rats were divided into four groups, subjected to different high-fat diets for ten weeks with varying nutrient components. The results showed that high-fat diet-induced hypercholesterolemia and hypertriglyceridemia significantly increased the plasma levels of β-thromboglobulin (β-TG), p-selectin, and platelet factor 4 (PF-4). The blood pressure readings were also elevated post high-fat diet induction. Interestingly, the group with the highest percentage of saturated fatty acid and trans-fat exhibited the highest PCSK9 levels, along with the highest increase in plasma cholesterol, triglycerides, and platelet activation parameters. These findings confirm that high-fat diet-induced hypercholesterolemia and hypertriglyceridemia stimulate platelet activity and PCSK9 levels. Moreover, our results suggest that PCSK9, implicated in hypercholesterolemia and hypertriglyceridemia, may synergistically mediate platelet hyperactivity, aligning with clinical studies. Notably, our results highlight the association between a high-fat diet and PCSK9, providing insights for drug discovery targeting platelet activation in atherosclerosis-induced cardiovascular diseases.

Implication of Mn-cofactored superoxide dismutase in the tolerance of swarmer Pseudomonas aeruginosa to polymixin, ciprofloxacin and meropenem antibiotics.

The protective role of superoxide dismutase (Sod) against oxidative stress, resulting from the common antibiotic pathway of action, has been studied in the wild type and mutant strains of swarmer Pseudomonas aeruginosa, lacking Cytosolic Mn-Sod (sodM), Fe-Sod (sodB) or both Sods (sodMB).Our results showed that inactivation of sodB genes leads to significant motility defects and tolerance to meropenem. This resistance is correlated with a greater membrane unsaturation as well as an effective intervention of Mn-Sod isoform, in antibiotic tolerance.Moreover, loss of Mn-Sod in sodM mutant, leads to polymixin intolerance and is correlated with membrane unsaturation. Effectivelty, sodM mutant showed an enhanced swarming motility and a conserved rhamnolipid production. Whereas, in the double mutant sodMB, ciprofloxacin tolerance would be linked to an increase in the percentage of saturated fatty acids in the membrane, even in the absence of superoxide dismutase activity.The overall results showed that Mn-Sod has a protective role in the tolerance to antibiotics, in swarmer P.aeruginosa strain. It has been further shown that Sod intervention in antibiotic tolerance is through change in membrane fatty acid composition.

Influence of bacterial strains and incubation conditions on the viability and quality of biological silage for use in animal production

The objective of this estudio was to determine the viability of biological silage (EB) using two native BAL strains of chicken or pigs, molecularly identified, as fillet residue raw material (Rp) Chelidonichthys obscurusor Diplectrum conceptione, incubated at room temperature (RT) or compared with 30°C and 40°C. The BALs were evaluated biochemically. For the EB, 70% (Rp), 25% (molasses) and 5% (BAL inoculum) were used. The EB viability was measured according to the pH and percentage of titratable acidity (%A), (according to day 3-5-10-15-30). In incubation at RT, EB using Lactobacillus brevisfrom pigs and Enterococcus aviumfrom chickens, with two Rp, was viable from day 10. In incubation at different temperatures, fermentation is affected at 40°C (faster). The stability from day 10 with an EB temperature of 32 °C, the amount of BAL and undesirable microorganisms, were within the acceptable limit for fermented food, the protein value from 33,8% to 36,7%. in D. conceptioneand 33% to 33,16% in C. obscurus. The raw material and incubation temperature modifies the process and final product where the BAL strains used in the incubation are indifferent to the fermentation process.

Comparison between Amino Acid Profiling of Structural Proteins of earliest and recent omicron strain of SARS-CoV-2 and Nutritional Burden on COVID-19 patients

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has four structural proteins. Inside human host cell, it replicates, for which each requires all the structural proteins, and to generate these, entire requirement of amino acids is borne by the host. There is no data regarding amino acid profiling, variation in strains, and its actual burden on the human host. Profiling, with its therapeutic utility, was studied using genomics and proteomics of earliest Wuhan strain and was compared with the recent emerging heavily mutated variant of concern, Omicron. With each replicating SARS-CoV-2 consuming a considerable percentage of essential amino acids (EAAs), coronavirus infection causes huge burden of EAAs on the entire human body in generating billions and billions of SARS-CoV-2 and can cause depletion of body protein stores. The complete amino acid profiling of the structural proteins of SARS-CoV-2 can act as benchmark for designing the correct nutritional supplements for the management of nutritional burden on the patients with SARS-CoV-2 infection.

Low and high temperatures promote docosahexaenoic acid accumulation in Crypthecodinium sp. SUN by regulating the polyunsaturated fatty acid synthase pathway and the expression of saturated fatty acid preferred diacylglycerol acyltransferases

Low (15 °C) and high (35 °C) temperatures significantly increased DHA as a percentage of total fatty acids (TFAs) to 43.6 % and 40.46 %, respectively (1.28- and 1.18-fold of that at 25 °C, respectively). The incompleteness of the FAS pathway indicates that DHA synthesis does not occur via this pathway. Meanwhile, Comparative transcriptome analysis showed that the PUFA synthase pathway might be responsible for DHA synthesis in C. sp. SUN. Additionally, the three diacylglycerol acyltransferases all had a substrate preference for saturated fatty acid (SFA)-CoA, which also contributed to the decreased SFA and increased DHA at both low and high temperatures. Additionally, WGCNA analysis identifies key regulatory genes that may be involved in temperature-regulated DHA proportion. The findings of this study indicate the mechanisms of temperature-regulated DHA accumulation in C. sp. SUN and shed light on the manipulation of DHA proportion by changes in temperature.

Galbibacter pacificus sp. nov., isolated from surface seawater of the western Pacific Ocean and transfer of Joostella marina to the genus Galbibacter as Galbibacter orientalis nom. nov. and emended description of the genus Galbibacter.

Two Gram-stain-negative, non-motile, non-spore-forming, strictly aerobic and rod-shaped bacterial strains, CMA-7T and CAA-3, were isolated from surface seawater samples collected from the western Pacific Ocean. Phylogeny of 16S rRNA gene sequences indicated they were related to the genera Galbibacter and Joostella and shared 95.1, 90.9 and 90.8% sequence similarity with G. mesophilus Mok-17T, J. marina DSM 19592T and G. marinus ck-I2-15T, respectively. Phylogenomic analysis showed that the two strains, together with the members of the genera Galbibacter and Joostella, formed a monophyletic clade that could also be considered a monophyletic taxon. This distinctiveness was supported by amino acid identity and percentage of conserved proteins indices, phenotypic and chemotaxonomic characteristics and comparative genomics analysis. Digital DNA‒DNA hybridization values and average nucleotide identities between the two strains and their closest relatives were 18.0-20.8 % and 77.7-79.3 %, respectively. The principal fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, Summed Feature 3 (C16 : 1 ω7c/C16 : 1 ω6c or C16 : 1 ω6c/C16 : 1 ω7c), Summed Feature 9 (iso-C17 : 1 ω9c or C16 : 0 10-methyl), and C15 : 0 3-OH. The predominant respiratory quinone was MK-6. The polar lipids were phosphatidylethanolamine, aminolipid, aminophospholipid, phospholipid, phosphoglycolipid, glycolipid and unknown polar lipid. The genomic DNA G+C content of strains CMA-7T and CAA-3 was both 38.4 mol%. Genomic analysis indicated they have the potential to degrade cellulose and chitin. Based on the polyphasic evidence presented in this study, the two strains represent a novel species within the genus Galbibacter, for which the name Galbibacter pacificus sp. nov. is proposed. The type strain is CMA-7T (=MCCC M28999T = KCTC 92588T). Moreover, the transfer of Joostella marina to the genus Galbibacter as Galbibacter orientalis nom. nov. (type strain En5T = KCTC 12518T = DSM 19592T=CGMCC 1.6973T) is also proposed.

SAT009 Investigating The Role Of Hepatic Small Heterodimer Partner In Regulating Gallstone Formation

Abstract Disclosure: N.M. Dang: None. S. Anakk: None. Nuclear receptor, Small Heterodimer partner (Shp) regulates bile acid homeostasis by inhibiting transcription of Cholesterol 7 alpha-hydroxylase (Cyp7a1), the rate-limiting enzyme involved in converting cholesterol to bile acids, within the liver. Bile acid regulation prevents the formation of gallstones as cholesterol is excreted into bile along with bile acids and phospholipids. How hepatic Shp affects the development of gallstones and the mechanisms underlying these effects remain unclear. Here we used a liver-specific knockout mouse model and a lithogenic diet model to uncover potential liver-to-gallbladder crosstalk that contributes to gallstone development. Previously, we had shown that the knockout of Shp alters bile acid composition, resulting in higher percentages of secondary bile acid, deoxycholic acid. This increased the hydrophobic index of hepatic bile acids and also implicated a trend for a subsequent increase in the hydrophobicity of biliary bile. Higher hydrophobicity is sufficient to increase susceptibility to developing gallstones by promoting cholesterol nucleation. Of note, the expression of transporter genes Abcg5 and Aqp1 were altered in the gallbladder of hepatic Shp knockout mice, indicating a potential change in their bile composition. The enterokine, Fgf15 is known to stimulate gallbladder filling through its interaction with its receptor, Fgfr4. Recently, the Fgf15-Fgfr4 axis was shown to prevent degradation of the SHP protein such that impeding this axis led to higher Cyp7a1 transcript, increased bile acid levels, and altered gallbladder filling. Taken together, these findings suggest that hepatic Shp acts as a central node that regulates bile acid transport, homeostasis, and composition within the gall bladder and thus can prevent gallstone development. Elucidating Shp-mediated liver-to-gallbladder crosstalk is important for understanding the contribution of liver dysfunction towards gallbladder diseases and gallstones. Presentation: Saturday, June 17, 2023

ULTRASONIC CAVITATION EFFECT ON THE FATTY ACID COMPOSITION OF LINSEED OIL-BASED EMULSIONS

Ultrasonic homogenization is a promising method of emulsion formation. Linseed oil containing polyunsaturated fatty acids in the optimal ratio was used as a control sample and the fat base of the emulsions. The effect of ultrasonic treatment can lead to a change in the percentage of fatty acids due to the acceleration and initiation of several chemical reactions. To assess the feasibility of using ultrasound in food production, the fatty acid composition of natural linseed oil and emulsions obtained from it, treated by ultrasound with different durations were studied. The study monitored the formation of radicals since increasing temperature and pressure during the collapse of cavitation bubbles initiates the formation of free hydrogen H- and hydroxyl OH- radicals within and next to them. These may initiate the oxidation of bioorganic compounds in the food product. The research results show that emulsions exposure to the ultrasound at a frequency of 20 kHz for 10, 20 and 30 minutes are capable to maintain the ratio of fatty acids while obtaining a homogeneous, finely dispersed and stable food emulsion. Deviations in the values characterizing the fatty acids content in the emulsions treated by ultrasound for 10, 20 and 30 minutes as compared with the control sample – linseed oil are insignificant (0.02±0.015% to 0.83±0.015). Using the method of electron paramagnetic resonance, the absence of free radicals was established both in the control sample – flax oil, and in emulsions obtained on its basis, homogenized using ultrasonic exposure.

Interactions of arsenic exposure, arsenic metabolism with red blood cell folate on the risk of hypertension: NHANES 2007-2016.

Arsenic exposure was associated with hypertension, and arsenic metabolism might be influenced by folate concentrations. Thus, this study aimed to explore the interaction between arsenic exposure and metabolism with folate concentrations on hypertension. We studied 6643 adults aged 20 years and older who participated in the National Health and Nutrition Examination Survey from 2007 through 2016. Urinary total arsenic (UTAs), the percentage of urinary dimethylarsinic acid (DMA%), serum and red blood cell (RBC) folate were collected. Logistic regression and restricted cubic spline (RCS) analyses were performed to determine the association and dose-response relationship. Interaction analyses were conducted on both additive and multiplicative scales. UTAs (median: 7.05μg/L) was positively associated with hypertension risk, and the adjusted OR was 1.44 (95% CI: 1.06-1.95) when comparing the third with the lowest quartile. And participants with the highest quintile of RBC folate had increased hypertension risk than those with the lowest quintile (adjusted OR=1.43, 95% CI: 1.06-1.94). Significant additive interaction was observed between excessive RBC folate with high UTAs (AP=0.323, 95% CI: 0.083-0.564) and low DMA% (AP=0.381, 95% CI: 0.119-0.643) on hypertension risk. Our results suggested significant interactions between high UTAs and low DMA% with excessive RBC folate on hypertension risk.

Synthesis of Cu-1,4-Benzene Dicarboxylate Metal-Organic Frameworks (Cu-BDC MOFs) from Plastic Waste and Its Application as Catalyst in Biodiesel Production

The increasing of PET plastic waste causes various environmental problems because decomposing it is difficult to compose. This study aims to utilize PET plastic waste as a source of terephthalic acid organic linker in the solvothermal synthesis of Cu-1,4-Benzene Dicarboxylate metal-organic frameworks (Cu-BDC MOFs). Cu-BDC MOFs were characterized using FTIR, XRD, and SEM-EDX. The characterization results showed that the crystals of Cu-BDC MOFs had an irregular cubic morphology with an average crystal size of 67.96 nm. Furthermore, Cu-BDC MOFs were applied as catalysts for the esterification reaction to produce biodiesel from coconut oil. The conversion percentage of free fatty acids in the esterification process was 43.75 %, while in the transesterification process, it was 68.90 %. Analysis using GC-MS showed the presence of 8 peaks, with the largest percentage of areas identified as fatty acid methyl esters. The major components were methyl laurate (31.17 %), methyl myristate (18.77 %), methyl palmitate (12.50 %), methyl caprylate (10.76 %), methyl elaidate (10.07 %), methyl caprate (7.34 %), methyl stearate (5.67 %), and methyl linoleate (2.26 %). The quality of the biodiesel was tested, and the results were as follows: The density parameter was measured at 842.5 kg/m3, the viscosity measurement showed 2.9572 cSt, and the water content was found to be 0.09 %. Additionally, the acid number parameter was determined to be 0.2799 mg-KOH/g. HIGHLIGHTS Cu-BDC MOFs are produced through the hydrolysis of PET waste using a solvothermal mixture of distilled water, ethanol, and DMF at 85 °C for 24 h. The micrograph of the synthesized Cu-BDC MOFs reveals a clustered topography of Cu-BDC MOFs crystals with irregular cubic (hexahedral) shapes. FTIR analysis of biodiesel displays distinctive peaks corresponding to the -C-H, -C=O, -CH2, -CH3, and -C-O functional groups. The GC-MS analysis of biodiesel from coconut oil shows the presence of 8 major peaks, namely methyl caprylate, methyl caprate, methyl laurate, methyl myristate, methyl palmitate, methyl linoleate, methyl elaidate, and methyl stearate, with the largest percent area. The quality test results of biodiesel indicate a density parameter of 842.5 kg/m3, a biodiesel viscosity measurement of 2.9572 cSt, a water content of 0.0898 %, and an acid number of 0.2799 mg-KOH/g. GRAPHICAL ABSTRACT

The antimicrobial peptide database is 20 years old: Recent developments and future directions.

In 2023, the Antimicrobial Peptide Database (currently available at https://aps.unmc.edu) is 20-years-old. The timeline for the APD expansion in peptide entries, classification methods, search functions, post-translational modifications, binding targets, and mechanisms of action of antimicrobial peptides (AMPs) has been summarized in our previous Protein Science paper. This article highlights new database additions and findings. To facilitate antimicrobial development to combat drug-resistant pathogens, the APD has been re-annotating the data for antibacterial activity (active, inactive, and uncertain), toxicity (hemolytic and nonhemolytic AMPs), and salt tolerance (salt sensitive and insensitive). Comparison of the respective desired and undesired AMP groups produces new knowledge for peptide design. Our unification of AMPs from the six life kingdoms into "natural AMPs" enabled the first comparison with globular or transmembrane proteins. Due to the dominance of amphipathic helical and disulfide-linked peptides, cysteine, glycine, and lysine in natural AMPs are much more abundant than those in globular proteins. To include peptides predicted by machine learning, a new "predicted" group has been created. Remarkably, the averaged amino acid composition of predicted peptides is located between the lower bound of natural AMPs and the upper bound of synthetic peptides. Synthetic peptides in the current APD, with the highest cationic and hydrophobic amino acid percentages, are mostly designed with varying degrees of optimization. Hence, natural AMPs accumulated in the APD over 20 years have laid the foundation for machine learning prediction. We discuss future directions for peptide discovery. It is anticipated that the APD will continue to play a role in research and education.

JcSEUSS1 negatively regulates reproductive organ development in perennial woody Jatropha curcas.

Overexpression of JcSEUSS1 resulted in late flowering, reduced flower number, wrinkled kernels, and decreased seed yield in Jatopha curcas, while downregulation of JcSEUSS1 increased flower number and seed production. The seed oil of Jatropha curcas is suitable as an ideal alternative for diesel fuel, yet the seed yield of Jatropha is restricted by its small number of female flowers and low seed setting rate. Therefore, it is crucial to identify genes that regulate flowering and seed set, and hence improve seed yield. In this study, overexpression of JcSEUSS1 resulted in late flowering, fewer flowers and fruits, and smaller fruits and seeds, causing reduced seed production and oil content. In contrast, the downregulation of JcSEUSS1 by RNA interference (RNAi) technology caused an increase in the flower number and seed yield. However, the flowering time, seed number per fruit, seed weight, and size exhibited no obvious changes in JcSEUSS1-RNAi plants. Moreover, the fatty acid composition also changed in JcSEUSS1 overexpression and RNAi plants, the percentage of unsaturated fatty acids (FAs) was increased in overexpression plants, and the saturated FAs were increased in RNAi plants. These results indicate that JcSEUSS1 played a negative role in regulating reproductive growth and worked redundantly with other genes in the regulation of flowering time, seed number per fruit, seed weight, and size.

Agronomic Comparisons of Herbicides with Different Active Ingredients and Mechanical Hoeing for Weed Control in Oleic and Linoleic Type Sunflower (Helianthus annuus L.) Hybrids

Weeds cause serious yield losses in sunflower production. The most common methods for weed control are herbicide application and mechanical hoeing. The objective of this study was to determine the effects of five traditional herbicides containing different active ingredients compared with mechanical hoeing for weed control on seed yield components, seed oil content, and fatty acid composition in sunflower. Field experiments were conducted in the sunflower growing seasons of 2014 and 2015 on farmer fields in Karamusul village of Lüleburgaz, Kırklareli, located in the Northwest of Turkey (40°24’ N, 27021 E and elevation 46 m). Pre-plant herbicide Bonoflan WG with benfluralin, pre-emergence herbicide Stomp®Extra with pendimethalin, and post-emergence herbicides Challenge600 with aclonifen and Targa Super with quizalofop-p-ethyl active were applied on non-Clearfield sunflower hybrids (P64LL05–Linoleic and P64H34–High oleic) while post-emergence herbicide Intervix®Pro with imazamox was applied on Clearfield hybrids (LG5542CL-Linoleic and Colombi–High oleic). In the results, the year effects were statistically significant on plant height, head diameter, number of days from planting to 50% flowering, and percentage of stearic acid of Clearfield cultivars while it was significant on plant height, head diameter, stem diameter, 1000 seed weight, number of days from planting to 50% flowering, seed yield, seed oil content, percentage of oleic and linoleic acids of non-Clearfield cultivars. The effect of genotype was significant for all seed yield and oil components except seed weight and seed yield for both groups’ cultivars. Intervix®Pro caused significant decreases in plant height, stem diameter, and percentage of stearic acid. Stomp®Extra decreased the number of days from planting to 50% flowering. Challenge 600 had a negative effect on the seed oil content of P64H34 in 2015. There was no significant difference between herbicide applications and mechanical hoeing for seed yields except for increasing seed yield of P64H34 by Bonaflan WG in 2015. For all herbicide applications, residue in seeds was not exceeded international acceptable limits. According to the results, herbicides especially post-emergence applications under stress conditions can adversely affect agronomic yield and seed oil components in sunflower.

Effects of Postharvest Chemical Preservatives on Shelf Life of Tomato (Lycopersicon esculentum cv. Srijana)

In recent years, the practice of increasing the shelf life of post-harvest crops is gaining attention worldwide due to the failure of proper techniques to increase post-harvest shelf life. Tomatoes are fragile and have a low shelf life. It fetches low market prices during on-season production and fetches high market prices during off-season production. To address this scenario, research was conducted to study the effect of different preservatives on various physiochemical attributes of tomato (Lycopersicum esculentum). The effects of preservatives were studied on shelf life, disease infestation days, total soluble solids (TSS), titratable acidity (TA), pH, and weight loss percentage (WLP) at 2-day intervals during the storage period. The 7 treatments used were 2% CaCl2, 4%, CaCl2, 1% GA3, 3% GA3, 1000 ppm sodium benzoate, 2000 ppm sodium benzoate, and control in distilled water with 3 replications each. Each replication was immersed in a chemical preservative for 20 minutes and kept in a polyethylene bag. Among the treatments, fruits treated with 3% GA3 recorded the longest shelf life of 31.33 days, followed by 1% GA3 (27 days) and 4% CaCl2 (22 days) over the control (15.667 days). Disease incidence days were highest for 3% GA3 (32.33 days) followed by 1% GA3 (28.33 days) and 4% CaCl2 (23 days) over control (16.667 days). The percentage of physical weight loss on the day of data recording was minimum for 3% GA3 treated fruits and maximum for control. Similarly, TA, TSS, and pH of treated fruits show significant results over control.

Cashew peduncle pulp in the production of Berliner Weisse craft beer: Sensory and physicochemical aspects

Among the extant beer types, Berliner Weisse is mainly characterized by its blend of barley and wheat malts, Saccharomyces cerevisiae for alcoholic fermentation, and Lactobacillus spp. for lactic fermentation. In this study, three formulations of Berliner Weisse were developed with various concentrations of barley, wheat malts, and hops. No variations were made in the concentrations of S. cerevisiae, Lacticaseibacillus casei, or cashew pulp. A L. casei-free formulation was used as a control. Physicochemical and sensory parameters were evaluated to characterize the formulations. The physicochemical data allowed for differentiation of the beverages in all evaluated parameters, except for the percentages of titratable acidity and diacetyl. From a sensory perspective, panelists classified the beer as acidic or fruity. The cashew peduncle pulp was proven to be a viable and attractive alternative for the production of Berliner Weisse-style beer with national characteristics and versatility in physicochemical and sensory parameters.

Putrescine, in Combination with Gibberellic Acid and Salicylic Acid, Improves Date Palm Fruit Quality via Triggering Protein and Carbohydrate Accumulation and Enhancing Mineral, Amino Acid, Sugar, and Phytohormone Acquisition

AbstractPutrescine (Put), gibberellic acid (GA3), and salicylic acid (SA) are involved in improving fruit growth and development. This is the first study investigating the effect of 1 mM Put, 100 mg L-1 GA3, and 100 mg L-1 SA on Phoenix dactylifera, cv. Zaghloul fruits. The experiment was conducted in a completely randomized block design during two successive seasons and five treatments [control (distilled water spray), Put, Put + GA3, Put + SA, and Put + GA3 + SA] were sprayed at the Hababouk (cell division) stage and Kimri [unripe green (cell elongation)] stage. Our results showed that all treatments significantly improved the yield and quality of ‘Zaghloul’ fruit by increasing the dry matter, crude fiber, ash, total soluble solids, (reducing, non-reducing, and total soluble) sugars, carbohydrate, protein, nitrogen, phosphorus, potassium, calcium, magnesium, sodium, zinc, iron, and manganese content as well as the peroxidase and catalase activity. Compared with the control treatment, exogenous applications also enhanced the amino acid (glutamic acid, aspartic acid, proline, glycine, alanine, arginine, cysteine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, valine) acquisition and phytohormone (indole-3-acetic acid, cytokinins, GA3, SA) content, while decreasing the percentage of moisture, total acidity, total phenols, and tannins. The novel evidence indicates that among all treatments, application of diamine (Put), in combination with phytohormones (GA3 and SA), has the greatest effect on improving ‘Zaghloul’ fruit yield by up-regulating the nutrient acquisition, sugar accumulation, amino acid profile, antioxidant response, and phytohormone performance. These findings support the use of Put in conjunction with GA3 and SA to improve fruit yield and quality.

Oryzibacter oryziterrae gen. nov., sp. nov., isolated from rice paddy soil.

A novel Gram-stain-negative, aerobic, motile and pleomorphic rod-shaped bacterial strain, designated COJ-58T, was isolated from rice paddy soil. Strain COJ-58T grew optimally at 20-30 °C, at pH 5.0-8.0 and with 0-1.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain COJ-58T forms a distinct lineage within the family Pleomorphomonadaceae, with highest similarity to Pleomorphomonas carboxyditropha SVCO-16T (95.9 %), Pleomorphomonas koreensis Y9T (95.8 %), Pleomorphomonas oryzae F-7T (95.7 %) and Pleomorphomonas diazotrophica R5-392T (95.6 %), respectively. The average nucleotide identity, digital DNA-DNA hybridization, average amino acid identity and percentage of conserved proteins values between the genomes of strain COJ-58T and its closely related taxa are ≤77.2 %, ≤21.6 %, ≤68.3 % and ≤61.3 %, respectively. The genome size of strain COJ-58T is 4.9 Mb and the genomic DNA G + C content is 63.7 mol%. The major fatty acids are C18 : 1 ω7c, C16 : 0 and summed feature 2 (C14 : 0 3-OH and/or iso-C16 : 1 I). The differential phenotypic and genotypic characteristics of strain COJ-58T indicate that it represents a novel genus and species, for which the name Oryzibacter oryziterrae gen. nov., sp. nov. is proposed, with strain COJ-58T (=KACC 22108T=JCM 34744T) as the type strain.

Optimization of chromatographic conditions via Box‒Behnken design in RP-HPLC-PDA method development for the estimation of folic acid and methotrexate in bulk and tablets

Simultaneous estimation of folic acid and methotrexate in bulk and tablet dosage form by RP-HPLC-PDA was conducted via Box‒Behnken design application. Three-factor numerical values were finalized from the graphical and numerical optimization with built-in ANOVA in BBD. Sharp and symmetric peaks were observed at 4.138 and 6.929 min for folic acid and methotrexate, respectively. The mobile phase composition was methanol and 0.1% formic acid in water with a ratio of 31:69 and a flow rate of 1.1 ml/min. Both drugs were detected at a wavelength of 291 nm. The developed method was validated according to ICH guidelines. The results of the validation parameters were within acceptable limits. Stress stability studies have been performed under acidic, alkali, oxidation, neutral and photolytic conditions. Three different brand-marketed tablets were assessed with the developed method (MGXT, FOLTNAX and TRUXOFOL). In the tablet formulations, chromatogram percentages of folic acid and methotrexate were calculated at 99.13% and 99.50 in MGXT, 99.17% and 99.47 in FOLTNAX, and 99.91 and 100.05 in TRUXOFOL.

Improving chemical, rheological and sensory properties of commercial low-fat cream by concentrate addition of whey proteins

The present study was conducted to determine the effect of whey protein concentrate (WPC) addition on the commercial low-fat cream's chemical, rheological and sensory properties. WPC was added to the low-fat cream (10% fat) in ratios of 1.0, 2.5, and 5.0% to represent the treatments C1, C2, and C3, respectively.In addition, a fat-rich, positive control treatment (C +) with a fat percentage of 30% and a negative low-fat control treatment (C-) with a fat percentage of 10% were investigated without adding WPC. Chemical tests were carried out, including the percentage of moisture, protein, fat, carbohydrates, total acidity, and pH, as well as rheological tests that included; hardness, springiness, and Cohesiveness. Also, a sensory evaluation was conducted. The results showed a decrease in the moisture percentage of the added treatments with the increment of the added WPC quantity. Also, a decrease in the fat percentage and pH of the WPC addition treatments was observed, combined with an increment in the percentage of protein, carbohydrates, ash, and total acidity. Regarding the microbiological properties, no bacterial or mycological contamination was observed during the manufacturing and storage periods. The results also showed the improvement of rheological and sensory characteristics by increasing the percentage of WPC addition compared with the positive and negative control treatments. The present study would be helpful in the production of low-fat cream fortified with whey proteins with high nutritional value.

Amelioration of Reproductive and Productive Performance in Animal and Poultry by Moringa Oleifera Free or Nano-Encapsulated

Improvement of reproductive performance is an honorable goal and is closely associated with increasing productivity. Therefore, as the reproductive efficiency improves, the herd net return and national income increase. The high cost of animal forage especially in dry or semi-arid countries, is a main limitation to animal production which is raised under extensive systems. Hence, utilization of alternative feed resources may be a reasonable and appropriate approach in low-input systems. Moringa Oleifera is a natural plant referred to as a nutrient, which contains substances that might have a favorable influence on animal reproduction and production. Leafy Moringa is considered a virtuous origin of proteins that are very consumable. Moreover, they are rich in Vitamin C, Vitamin A, iron, and Calcium which are necessary for the growth of livestock animals. Moreover, they include the percentage of fatty acid which is greater as compared to any woody plant fodder. Principally, almost all of the fatty acids present in Moringa are unsaturated fatty acids which are highly susceptible. Nowadays, nano-encapsulation technology is considered a promising strategy for drug delivery and is efficient in preserving bioactive components in products rich in nutrients. So that this review affords a collection of significant data and enlightens the information on the development of Moringa Oleifera Free or its nano-encapsulated form in amelioration of animal and poultry reproduction and production performance.