Percentage Of Protein Content Research Articles

Effect of Seed Treatment of Boron, Cobalt and Molybdenum on Growth Parameter, Grain Yield and Seed Quality in Soybean (Glycine max L.)

This study aimed to evaluate the effect of seed treatment of boron, cobalt and molybdenum on growth parameter, grain yield and seed quality in soybean (Glycine max L.) during kharif, during July, 2022 at experimental farm, department of Soil Science and Agricultural chemistry, College of Agriculture, Badnapur. The experiment was laid out in Randomized Block Design (RBD) with 8 treatments which were replicated thrice.The treatments comprises (T1) Absolute control, (T2) RDF, (T3) RDF + Boron @ 2 gm kg-1 seed, (T4) RDF + Cobalt @ 1.5 gm kg-1 seed, (T5) RDF + Ammonium molybdate 1 gm kg-1 seed , (T6) RDF + Boron @ 2 gm kg-1 seed + Cobalt @ 1.5 gm kg-1 seed, (T7) RDF + Cobalt @ 1.5 gm kg-1 seed + Ammonium molybdate @ 1 gm kg-1 seed, (T8) RDF + Boron @ 2 gm kg-1 seed + Cobalt @ 1.5 gm kg-1 + Ammonium molybdate @ 1 gm kg-1 seed .To achieve the objectives the observations were recorded at various growth stages of soybean kharif, during 2022. The result indicates that, treatment (T8) receiving RDF + Boron @ 2 gm kg-1 seed + Cobalt @ 1.5 gm kg-1 seed + Ammonium molybdate @ 1 gm kg-1seed was found effective due to advantages effect on morpho-physiological growth, yield and yield attributing characters likeplant height, nodulation, fresh weight of nodules, no. of root nodules, root length, number of pods plant-1of soybean grain yield, Stover yield of soybean. In grain quality the results revealed that test weight, protein content percentage were significantly influenced with the application of treatment T8. On the other hand, in all the cases the lower response was found from the control treatment.

Physicochemical, Cooking Quality and Sensory Acceptability of Three Cowpea Varieties Grown in Botswana

Background: Information on the physicochemical, cooking and sensory qualities of cowpea seeds are required for nutrition information, to design processing and for consumer acceptance. In view of this, three cowpea varieties (Tswana, Blackeye and ER7) grown in Botswana were evaluated. Method: Proximate compositions, physical, gelatinization and sensory properties respectively were evaluated following AOAC, conventional, Amylograph and on 7-point hedonic sensory acceptability scale. Result: Significant variations (p less than 0.05) were observed in fat, fiber, ash and available carbohydrate (CHO) contents, length, thickness, hundred seeds weight (HSW), hydration capacity, hydration index, cooking time, flour starches maximum gelatinization temperature (Tm,°C) and viscosity at Tm. The percentage moisture, protein, fiber, fat, ash and available carbohydrate contents ranged 9.5-10.1, 21.7-22.8, 2.3-2.6, 1.1-2.0, 2.1-3.0 and 59.4-62.3, respectively. Initial gelatinization temperature (To,°C) and Tm (°C) were ranged 71.3(ER7)-73.7(Tswana) and 83.0(ER7)-84.7(Blackeye), respectively. Significant (p less than 0.05) variations in Tm and viscosity at Tm were observed because of differences in water uptake, swelling and thickening ability at high temperature. Longest cooking time was recorded for Tswana (43.57 min) while shortest was for Blackeye (36.10 min). Tswana recorded a longer cooking time probably because of low water hydration capacity, small seed size and low HSW. There was no significant difference in the sensory liking of the cooked cowpea seeds and were rated from slightly to moderately like. Cowpeas of high protein and fiber contents, short cooking time and high thickening ability on cooking are preferred for healthy diets. In terms of cooking time and thickening ability, Tswana variety appeared poor. Shorter cooking time, high protein content and high thickening ability of Blackeye cowpea is more acceptable. The ER7 is preferred for its large seed size.

Boosting Nutritional Proficiency of Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Agaricomycetes): A Selenium and Zinc Fortification Exploration.

The present study was aimed at evaluating the nutritional characteristics of bio-enriched oyster mushrooms. Cereal, leguminous and oilseed waste was used as substrates. Rice, soybean and mustard straw performed the best among all substrate fortified by adding Se and Zn salts individually and in combination at three different doses (25, 50 and 75 mg/kg) for nutrient analysis. The results showed that highest carbohydrate, 54.01 percent and protein content, 29.41 percent after fortification with Se and Zn at 75 mg/kg as compared to other doses on different substrates. Se at 75 mg/kg gave maximum carbohydrate (60.29%) and protein content (32.32%) in soybean straw while Zn supplementation at 50 mg/kg resulted in high fat content (2.74%) while rice straw had maximum fiber content (18.12%). Based on maximum yield, best dose of both the salts (25 mg/ kg of substrate) were combined and evaluated. The results indicated that soybean straw had the highest nutrient contents having 55.00 percent carbohydrate and 27.72 percent protein content. It was concluded that fortification with selenium and zinc increased the nutritional composition of Pleurotus ostreatus in comparison to control.

Peningkatan Kadar Serat Brownies Kukus dengan Penggunaan Tepung Kacang Merah (Phaseolus vulgaris L.)


 
 
 Brownies are a type of dark brown cake with a soft texture, a distinctive chocolate aroma and a sweet taste. Generally, flour-based brownies have a low fiber content. Therefore, red bean flour is expected to be used to increase fiber content. The purpose of this study was to determine the effect of using red bean flour on the fiber content of steamed brownies and produce steamed brownies with high fiber content and the best characteristics. This study used a completely randomized design (CRD) with the treatment ratio of wheat flour and red bean flour, namely: 100:0, 90:10, 80:20, 70:30, and 60:40 repeated 3 times to obtain 15 experimental units. The data obtained were analyzed by Analysis of Variance (ANOVA), followed by the Duncan Multiple Range Test (DMRT). The results showed that the use of red bean flour had a significant effect (P<0.05) on crude fiber content, moisture content, ash content, protein content, fat content, carbohydrate content, and sensory properties of steamed brownies, but had no significant effect (P > 0.05) on the sensory properties of the color of steamed brownies. Treatment of 70 percent wheat flour and 30 percent red bean flour was the best treatment with 5.08 percent crude fiber content, 23.66 percent moisture content, 2.20 percent ash content, 8.84 percent protein content, 33.81 percent fat content, carbohydrate content of 31.46 percent with color, aroma, texture, taste and overall acceptance favored by the panelists.
 
 

Impact of different levels of foamed sulfur and Nitrogen fertilizer (Urea) on soil reaction (pH) and yield of wheat (Triticum aestivum L.) cultivar (IBAA 99)

An experiment was conducted in one of the agricultural lands of Al-Muthanna Governorate for the agricultural season 2018_2019, to find out the effect of adding one of the soil conditioners represented by foamed sulfure by three levels (0, 1000 and 2000) kg S ha-1 , addition of nitrogen fertilizer (Urea) CO(NH2)2 at three levels (200, 400 and 600) kg N ha-1 to the soil in the degree of soil reaction (pH) and the yield of wheat cultivar (IPAA 99). The treatments within the experimental units were arranged according to the Randomized Complete Block Design (RCBD) with four replicates, so the total experimental units were 36 experimental units, where the level of addition of 2000 kg N ha-1 of foamed sulfur and the level of 600 kg N ha-1 of nitrogen fertilizer (Urea) were significantly higher, to give them the lowest averages for the soil reaction (pH) of 7.13 and 7.38 at germination and 7.19 and 7.29 at the flowering stage for each of the sulfur and nitrogen fertilizers, respectively, compared to the comparison treatment. Both levels were significantly superior in giving them the highest averages for some growth characteristics, and the yield amounted to(343 and 304 spikes m-2 , 65 and 62 grains of spike-2 , 43.58 and 42.20 gm, and 4.88 and 478 tons ha-1 and 25.60 and 24.32%, for the characteristics spikes numbers, the grains spike number, 1000 grain weights, grains yield and the protein content percentage in grains, respectively, for both levels. Keywords: foamed sulfur, Nitrogen fertilizer (Urea), soil reaction (pH), yield, wheat (Triticum aestivum L.), IBAA 99cultivar.

Effect of perinatal dietary protein deficiency on some neurochemicals and cytoarchitectural balance, in F1 and F2 generations of rats

ABSTRACT Protein deficiency, characterized by an inadequate intake of protein in the diet that fails to meet the body’s physiological requirements across various stages, can lead to detrimental outcomes. This is of interest due to the persistent low protein content in staple foods and suboptimal dietary patterns. The study sought to assess the intergenerational repercussions of dietary protein deficiency on specific neurochemicals and the cytoarchitecture of the brain within the F1 and F2 generations of rats. The rats were categorized into four groups based on the protein content percentage in their diets: 21% protein diet (21%PD), 10% protein diet (10%PD), 5% protein diet (5%PD), and control diet. Neurobehavior was assessed, while brain serotonin and dopamine levels were measured using HPLC. BDNF and GDNF expression in the hippocampal and prefrontal (PFC) sections, Immunohistochemical investigations of the morphological impact on the hippocampus and PFC, were also analyzed. The protein-deficient groups displayed anxiety, loss of striatal serotonin and increased dopamine levels, degenerated pyramidal cells in the hippocampus, and a prominent reduction in cellular density in the PFC. BDNF and GDNF levels in the PFC were reduced in the 5%PD group. GFAP astrocyte expression was observed to be increased in the prefrontal cortex (PFC) and hippocampal sections, indicating heightened reactivity. The density of hypertrophied cells across generations further suggests the presence of neuroinflammation. Changes in brain structure, neurotransmitter levels, and neurotrophic factor levels may indicate intergenerational alterations in critical regions, potentially serving as indicators of the brain’s adaptive response to address protein deficiency across successive generations.

A review and meta-analysis of selected plant protein sources as a replacement of fishmeal in the diet of tilapias

Abstract Numerous studies on the replacement of fishmeal with plant protein sources in tilapias have been undertaken. In order to quantify the effect of replacing fishmeal with plant protein sources on the growth performance of tilapias, a meta-analysis approach was applied. Despite the high heterogeneity and funnel plot asymmetry, the meta-analysis showed that replacing fishmeal with plant protein sources has a significant positive effect on the growth performance of tilapias. Thus, tilapias appear to be preadapted to utilizing plant protein sources. Furthermore, the feed value, nutritional quality and cost of the commonly used plant ingredients (soybean, sunflower, canola, cottonseed, kikuyu and azolla meals) were explored. The Solver function in Excel was used to formulate least-cost diets using the plant meals. Azolla had the highest nutritional index (9.7436). This was attributed to its excellent amino acid profile that exceeded the requirements of tilapias. Nutritional index and feed value were lowest in kikuyu because of its poor amino acid profile as it registered the lowest amino acid index (0.4918). These results indicate that the amino acid profile is more important in the determination of nutritional quality than the percent protein content. Azolla and soybean meal are good candidates for the replacement of fishmeal in the diets of tilapias.

Effects of Potassium Application on Yield, Protein and Oil Content of Selected Soybean Varieties in Trans Nzoia County, Kenya

There is low soybean production due to poor agronomic techniques, such as sub-optimal application of fertilizer among other factors. There are limited studies on the effects of potassium fertilizer application which is crucial in productivity, quality, and drought resistance. A study was conducted at the Kenya Agricultural Livestock and Research Organization, Kitale demonstration farm to determine the effects of potassium fertilizer application on the yield and quality of soybean varieties. A 3 x 4 factorial experiment was laid down in Randomised Complete Block Design and replicated three times. The study had two factors, that is three soybean varieties (DPSB19, Gazelle and SB24) and four levels of potassium (0 [control], 22.5, 45, and 67.5 kg K2O ha-1) applied as sulphate of potash fertilizer (50% K2O) at planting, making a total of 12 treatments. Data collected included number of pods per plant, number of seeds per plant, weight of seed per plant and 1000-seed weight, grain yield, protein and oil content. Data collected was subjected to analysis of variance using Statistical Analysis System version 9.3 and significantly different means separated using Least Significance Difference at α = 0.05. Application of potassium at the rate of 67.5 kg K2O ha-1 resulted to higher number of pods per plant and number of seeds per plant at 78.97 and 243.40, and 44.47 and 141.20 in the varieties DPSB19 and Gazelle, while in SB24 higher recording of 51.23 and 162.40 was observed with 22.5 kg K2­O ha-1. The higher 1000-seed weight was recorded in 22.5 kg K2O ha-1 in DPSB19 and Gazelle varieties, while for SB24 it was recorded in 67.5 kg K2O ha-1. The highest grain yield recorded was 9156.45 kg per ha, in DPSB19 in 22.5 kg K2O ha-1, 4517. 67.5 kg per ha in Gazelle variety in 67.5 kg K2O ha-1 and 6432.78 kg per ha in SB24 variety in 22.5 kg K2O ha-1. The higher percentage oil content was observed in 45 K2O kg ha-1 in DPSB19 (28.48%) and SB24 (23.38%) varieties, and in 22.5 kg K2O ha-1 in Gazelle (19.38%) variety and the higher percentage protein content was recorded in 45 K2O kg ha-1 in DPSB19 (42.99%) and Gazelle (40.56%), and in 45 K2O kg ha-1 in SB24 (48.87%). These findings show that soybean farmers should apply potassium fertilizer for appropriate growth, increased yield, protein and oil content in different soybean varieties. It is recommended that application of potassium at 67.5 kg K2O ha-1 be done for better soybean growth, yield and protein content and application of potassium at 45 kg K2O ha-1 is done for high oil content.

The Effect of Different Steaming Temperatures on Albumin Levels of Scad Fish (Decapterus ruselli)

Albumin is a plasma protein that consists of a single polypeptide chain and is used to accelerate the recovery of split/damaged cell tissue. Albumin is produced from human blood in the form of human serum albumin (HSA) which is fulfilled through imports at a relatively expensive price. The alternative albumin is needed to get the cheaper albumin but has the same clinical aspects, such as albumin from the scad fish (Decapterus ruselli). The purpose of this study is to determine the effect of different steaming temperatures on the albumin levels of scad fish (Decapterus ruselli) and get the optimal steaming temperature used to obtain albumin levels from scad fish (Decapterus ruselli). This research was conducted by the Completely Randomized Design (CRD) method which consists of 4 different steaming temperature treatments with 5 replications. The parameters observed were albumin content, protein content, and yield. The parameters were tested using One Way Analysis of Variance (ANOVA) followed by the Duncan Multiple Range Test. The results of this study showed that the treatment of different steaming temperatures had a significant effect (p<0.05) on the albumin levels and percentage of protein content. The optimum steaming temperature to obtain the highest albumin content from scad fish species Decapterus ruselli is 35℃ with an albumin content of 0.1394 g/L. Keywords: Scad fish, steaming, albumin, protein, yield

Comparison of milk and grass composition from grazing Irish dairy herds with and without milk fat depression

BackgroundThis study investigated the factors relating to pasture chemical and fatty acid (FA) composition that influence the milk fat percentage of spring calving, grazing dairy cows. The relationship between milk fat percentage and FA composition of the milk in these herds was also investigated.ResultsMilk protein percentage, milk casein percentage and cheddar cheese yield were increased in milk from HMF herds. Cows from LMF herds did not have negatively altered milk processability including rennet coagulation time (RCT), pH and ethanol stability. Crude protein, NDF, ADF, ether extract and total FA content of pasture was not different between LMF and HMF herds. Milk fat concentration of conjugated linoleic acid (CLA) t10, c12 was not different between HMF and LMF herds. Pre-grazing herbage mass and pasture content of crude protein, neutral detergent fibre (NDF) and total FA were similar between HMF and LMF herds. Pasture offered to LMF herds had a higher concentration of monounsaturated fatty acids (MUFA). A strong negative relationship (r = -0.40) was evident between milk fat percentage and pasture crude protein content for MMF herds (3.31–3.94% milk fat).ConclusionsThis research reports improved milk protein percentage, milk casein percentage and cheddar cheese yield from HMF herds compared to LMF herds. Milk processability was not impacted by low milk fat percentage. Pasture NDF and total fatty acid content was similar in HMF herds and LMF herds. Milk fat percentage had a strong negative association (r = -0.40) with pasture crude protein content in MMF herds (MF 3.31–3.94%). Correlation values between pasture chemical and FA composition and milk fat percentage in LMF herds and HMF herds were low, indicating that diet is not the only causative factor for variation in milk fat of grazing dairy cows. Comparison of milk fatty acid composition from herds with and without milk fat depression suggests that there may be other fatty acids apart from CLA t10, c12 that contribute to the inhibition of milk fat synthesis during milk fat depression in grazing herds.

Effect of summer legumes incorporation on quality parameters of succeeding Kharif rice

In Navsari, Gujarat, at the College Farm of Navsari Agricultural University, a field experiment was conducted in the summer and kharif of 2021 and 2022. With 4 main plots and 6 subplot treatments, the experiment comprised of twenty-four treatment combinations that were replicated 3 times in a split plot design. Results posed that significantly higher milling quality parameters and protein content of rice was found when rice grown in dhaincha incorporated plots (T3) and it was followed by green gram (T1), cowpea (T2) incorporated plots, and significantly lower values were seen in the fallow treatment (T4) for both years and in the pooled analysis. However, amylose and amylopectin content in rice was non-significant during the study. In the case of recommended levels tested during kharif in rice, significantly higher values of milling quality parameters, amylose, amylopectin, and protein content percentage were higher with application of 100% RDF (W1) and fb 75% RDF + 25% N from FYM (W4) during 2021-2022 and in pooled basis. Whereas the treatment No-fertilizer application (W6) recorded lower values throughout both the years and on a pooled basis. The experimentation revealed that dhaincha incorporation and 100% RDF proved as better in enhancing milling quality attributes and protein content whereas amylose and amylopectin percentages did not vary under residue incorporation.

The relationship between the end of the vegetation period and fruit properties in the walnut population in Eastern Serbia

The main objective of the study was to examine the walnut population from the region of Eastern Serbia for determining the relationship between the end of the vegetation period and fruit properties (fruit weight, kernel percentage, oil and crude protein contents). The walnut trees showed highly significant differences in the tested parameters. The walnut population was dominated by trees with a medium end of the vegetation period, moderate resistance to low temperatures (39.5%) and very small fruits (23.5%). The fruits of these trees had ? high kernel percentage (15.8%) and moderate oil and crude protein contents (25.8% and 38.3%, respectively). Contrary, the walnut population comprised the fewest trees of late ending of the vegetation period, with extremely large fruits (0.1%), exceptional kernel percentage (0.2%) and pronouncedly high oil and crude protein contents (0.07% and 0.5%, respectively). The studied walnut trees show great variability in the date of the end of vegetation and fruit properties, which indicates a high potential of the population in the selection of new genotypes, and the necessity for their conservation for further breeding, propagation and commercial cultivation.

Evaluation of light-emitting diode colors and intensities on slaughter performance, meat quality and serum antioxidant capacity in caged broilers.

This study was to evaluate the interaction of three different light-emitting diode (LED) light colors (white, green, and blue) and three intensities (5, 10, and 15 lx) on slaughter performance, meat quality and serum antioxidant capacity of broilers raised in three-layer cages. A total of 648 (8-days-old) male broiler chicks (Cobb-500) were randomly assigned in 3×3 factorially arranged treatments: three light colors (specifically, white, blue, and green) and three light intensities (namely, 5, 10, and 15 lx) for 35 days. Each treatment consisted of 6 replicates of 12 chicks. The test lasted for 35 days. The semi-eviscerated weight percentage (SEWP) in 5 lx white was higher than that in 15 lx (p<0.01). The eviscerated weight percentage (EWP) (p<0.05) and water-loss percentage (WLP) (p<0.01) decreased in 10 lx white light than those in green light. Under blue light, the content of hypoxanthine (Hx) in muscle was lower than that under white and green light (p<0.01). The content of malondialdehyde (MDA) in 15 lx blue light was higher than that in 10 lx green light (p<0.05). Light color had an extremely significant effect on thigh muscle percentage, WLP, Hx, and crude protein content (p<0.01). Light intensity had a significant effect on SEWP (p<0.05), EWP (p<0.05), lightness (L*) value (p<0.05), WLP (p<0.01), and the contents of superoxide dismutase (p<0.05), MDA (p<0.01), glutathione peroxidase (p<0.01). Using white LED light with 10 lx light intensity can significantly improve the chicken quality of caged Cobb broilers, improve the content of inosine acid in chicken breast and enhance the antioxidant capacity of the body. We suggest that the broiler farm can use 10 lx white LED light source for lighting in 8 to 42 days.

Variations of Nutrient and Antinutrient Components of Bambara Groundnut (Vigna subterranea (L.) Verdc.) Seeds

Bambara groundnut (BGN) fits the bill when it comes to an acceptable level of nutrient and mineral composition. BGN is a balanced food that can help eradicate food and nutritional insecurity if it is incorporated into the major food system. However, there is a large degree of variation in nutrient composition and antinutritional factors among BGN accessions. Here, we show the degree of variability of nutrient and antinutrient components such as percentage ash, moisture, protein, fat, tryptophan, tannin, and phytate contents in seeds of 95 accessions of BGN. Data were subjected to analysis of variance (ANOVA), followed by correlation and principal component analysis. Clustering was done to show the relatedness between the accessions in response to the various traits. A high level of heterogeneity was observed among the accessions for the various traits studied. PC1 and PC2 show 41.2% of the total observed variations. Cluster analysis grouped accessions into four main clusters. This study was able to confirm the high level of diversity in the components of nutrients and antinutrients previously reported in BGN. The results of this study are expected to aid in identifying parent lines for improved breeding programs.

Physicochemical and adulteration study of fresh milk collected from different locations in Pakistan

The present research was carried out to assess raw milk's quality as collected from the commercial markets of the Mardan district (Khyber Pakhtunkhwa). The locality from which milk samples were collected included; Bijligar (BG), Manga (M), Chamthara (CM), Main Bazar Mardan (MB), Mahidherai (MD), and Sharif Abad (SB), located in district Mardan, Khyber Pakhtunkhwa (KP). A total of 36 milk samples were collected at the rate of 6 samples per location. The outcome of the data exhibited that the percentage of protein content was highest (3.34%) in MB and SA (3.30%), while lower percentages were recorded in sample M (3.03%) and CM 93.06%). Maximum pH were shown in M and BG to be 7.55 and 7.33, respectively. For fats content, the highest percentage of fats was witnessed in MB as 4.04%, and minimum fats content was noted in Sample M as 3.57%. Water content was highest in Sample SA and BG at 15.85% and 15.64%, respectively. Qualitative analysis of adulterants like detergents, Formalin, starch, and Hydrogen peroxide was also carried out for all the collected samples. Adulteration results were positive for all the milk samples, with the highest being in samples MB (30%), while all the remaining samples had adulteration at 20% each. Both MB and CM samples were adulterated with urea, while the remaining 4 were adulterated with neutralizers. Thus, it may be summarized from the whole analysis that the milk available in commercial markets of district Mardan was adulterated with different adulterants and is not recommended for consumption.

Potential of selected plant essential oils in management of Sitophilus oryzae (L.) and Rhiyzopertha dominica (F.) on wheat grains

BackgroundTwo plant essential oils, basil (Ocimum basilicum L.) and petitgrain mandarin (Citrus reticulate L.), and one pyrethroid chemical insecticide, lambda-cyhalothrin, were evaluated against two of the most important stored product insect pests, Sitophilus oryzae and Rhyzopertha dominica, on wheat grains under laboratory conditions using mixing with feeding method. The effect of the tested materials was studied on F1 progeny of the tested insects. Chemical components of the oils and side effects on protein content percentage, total carbohydrates of wheat grain and germination were investigated.ResultsResults obtained showed that the two oils had a good insecticidal effect on the tested insects. Petitgrain mandarin oil was the premier. The two tested essential oils reduced the F1 progeny. In addition, the progressive of periods after storage (0–180 days) significantly reduced protein content (%) and total carbohydrates compared to control. Nearly, there were no significant differences between treatments (LC50 and LC95) of basil and (petitgrain) mandarin on the studied parameters. Germination percentages from 0 to 180 days post-storage had slight differences between control and treatments for oils. Lambda-cyhalothrin significantly reduced germination, especially with the LC95 from 60 to 180 days. Chemical analysis demonstrated that the main components of basil oil were linalool, eugenol, methyl chavicol and cani, while the main components of petitgrain mandarin oil were dimethyl panthenalate, α-terpinene, β-cinem and citral accounting for 71.1 and 95% of the two oils, respectively.ConclusionsThe results obtained proved that the two tested plant essential oils had an obvious role in suppressing the activity of the two tested insects and can be used in an integrated pest management program. The two tested essential oils can be used as green pesticides and can be effective alternatives for chemical pesticides.

A Comparative Study on Total Muscle Protein Content of Different Fish Species in Fresh and Smoked - Dried Condition and to Analyze their Banding Pattern through SDS- PAGE

Fish plays an important role in human nutrition. They are known to be a very healthy food item. They are excellent protein sources that also deliver various minerals and vitamins necessary for good health. Due to the high content of polyunsaturated fatty a cid, fish flesh and fish oil are beneficial in reducing the serum cholesterol. In addition to that, the special type of fatty acid, omega -3 polyunsaturated fatty acid, is recognized as an important component to prevent a number of coronary heart diseases. Fish proteins contain all the essential amino acids in the required proportion and hence have a high nutritional value, which contribute to their high biological value. In fresh fi sh muscles, the water content is strongly bound to the proteins and cannot be easily removed even under higher pressure. For the present study, three different fish species, Clarias batrachus (E1), Mystus tengara (E2) and Puntius ticto (E3) were taken to study their protein content and analyze the electrophoretic protein banding pattern for both fresh and smoked dried conditions. Pr otein extract was made from the muscle tissue of the fishes taken. The protein content was measured by Lowry’s method using BSA protein as standard. The optical density was measu red at 660nm. The electrophoretic protein banding patterns were determined using SDS-PAGE gel electrophoresis. The results showed that the total protein concentration of samples for fresh condition E1F, E2F and E3F was found to be 7.15±0.247 mg/ml, 7.20±0.224 mg/ml and 4.98±0.283 mg/ml respectively and for smoked dried condition E1D, E2D and E3D was 8.48±0.273 mg/ml, 8.31±0.222 mg/ml and 5.81±0.335 mg/ml respectively. The SDS-PAGE electrophoresis showed significant increase in the protein bands of three types of smoked dried fish samples when compared to fresh ones. Therefore, the percentage of protein content in selected smoked dried fishes was found more.&#x0D;

Influence of Treated Wastewater on the Percentage of Protein Content during Fodder Intercropping

This study aims to explore the potential use of treated wastewater in irrigating fodder crops and its effects on protein contents. A comparison of the protein contents in intercropped fodder plants irrigated with fresh water, and rainfall water, against those irrigated with treated grey water was performed under Palestinian climate conditions. Field experiments with different intercropping mixing ratios were carried out in 2017–2019 at the National Agricultural Research Centre in Palestine (NARC). Measurements of the nutritional value of each mixture specifically the protein contents were carried out to get the optimal and best conditions for preparing animal feed crops with three different water sources used. For alfalfa with vetch, the best result for protein percentages was (on average) obtained from the rain-fed experiment (17.1% protein) followed by the freshwater experiment (12.9% protein) and then by the treated grey-water experiment (12.6% protein). It appears that the best result for alfalfa with barley for protein percentages was (on average) obtained from the treated grey-water experiment (13.0% protein) followed by the freshwater experiment (11.1% protein) and then by the rain-fed experiment (10.5% protein). Statistical analysis of the data showed that percent protein for each specific mixing ratio resulted in significant differences in the protein % for the those irrigated with fresh water compared with the other types of water. The highest protein % was found to be for that irrigated with fresh water (31.9 for 10/90 alfalfa/barley ratio) followed by that irrigated with treated grey water (28.4 for 20/80 alfalfa/barley ratio) and then for the 30/70 ratio irrigated with treated wastewater (22.5%), and then for the 100/0 ratio of alfalfa/barley irrigated with rainwater (19.0). Overall, results of this study showed that cereal-legume intercropping irrigated with treated grey water can be used as a suitable management strategy for producing high-quality and high-quantity forage. Furthermore, the use of treated water can reduce the already strained demand on fresh water due to increase in population among other factors.

Relationship between Grain Yield and Quality Traits under Optimum and Low-Nitrogen Stress Environments in Tropical Maize

Breeding for nitrogen use efficiency (NUE) is important to deal with food insecurity and its effect on grain quality, particularly protein. A total of 1679 hybrids were evaluated in 16 different trials for grain yield (GY), grain quality traits (protein, starch and oil content) and kernel weight (KW) under optimum and managed low soil nitrogen fields in Kiboko, Kenya, from 2011 to 2014. The objectives of our study were to understand (i) the effect of low soil N stress on GY and quality traits, (ii) the relationship between GY and quality traits under each soil management condition and (iii) the relationship of traits with low-N versus optimum conditions. From the results, we observed the negative effects of low N on GY, KW and the percentage of protein content, and a positive effect on the percentage of starch content. The correlation between GY and all quality traits was very weak under both soil N conditions. GY had a strong relationship with KW under both optimum and low soil N conditions. Protein and starch content was significantly negative under both optimum and low-N conditions. There was no clear relationship among quality traits under optimum and low N, except for oil content. Therefore, it seems feasible to simultaneously improve GY along with quality traits under both optimum and low-N conditions, except for oil content. However, the negative trend observed between GY (starch) and protein content suggests the need for the regular monitoring of protein and starch content to identify varieties that combine both high GY and acceptable quality. Finally, we recommend further research with a few tropical maize genotypes contrasting for NUE to understand the relationship between the change in grain quality and NUE under low-N conditions.

Identification of growth‐related indicators affecting the appearance and protein content of rice grains

AbstractTo facilitate the stable production of rice (Oryza sativa L.) in a changing climate, it is imperative to establish optimal N application rate via growth diagnosis. In this study, field experiments were conducted to identify useful indicators of white‐back grains and protein content of rice. During the reproductive stages in 2018 and 2019, we applied N at different rates and recorded the soil and plant analyzer development (SPAD) readings and normalized difference vegetation index (NDVI) measurements. The white‐back grain percentage and grain protein content were more strongly correlated with SPAD readings and NDVI during the reproductive stage when the 2018 and 2019 data were combined than with the separate data for each year. In the combined 2018 and 2019 data, SPAD readings were the most dominant factor affecting white‐back grain percentage at 4–2 wk before heading, whereas NDVI was the most dominant factor at 1 wk before heading. Similarly, in the combined 2018 and 2019 data, SPAD readings were the most dominant factors for grain protein content on all measurement days, and NDVI was one of the dominant factors at 1 wk before heading. These results suggest that SPAD readings at 4–2 wk before heading and SPAD readings and NDVI at 1 wk before heading are useful indicators for determining the optimal N application rates during the reproductive stage to reduce the occurrence of white‐back grains and to regulate the grain protein content.