One way to increase the production and quality of cayenne pepper seeds is by proper fertilization. The purpose of this study was to obtain the best concentration of NPK (16-16-16) fertilizer for the production and quality seeds of Bonita and Loblita varieties. This study used a nested design with a basic design of randomized group design consisting of 4 levels of NPK (16-16-16) fertilizer concentrations (0 g/l (control), 5 g/l, 10 g/l, and 20 g/l) and 2 varieties of cayenne pepper (Bonita and Loblita) using 3 replications. The concentration of 5 g/l NPK fertilizer (16-16-16) on the Bonita variety gave the best results on germination (85,33%), seed growth speed (24,73%/ethmal), and seed growth uniformity (84,33%). While in the Loblita variety, the concentration of NPK fertilizer (16-16-16) 10 g/l gives the best results on germination (83%), seed growth speed (11,31%/ethmal), and seed growth uniformity (64,33%).