Peanut Production Research Articles

Immobilizing white-rot fungi laccase: Towardbio-derived supports as a circular economy approach in organochlorine removal.

Despite their high persistence in the environment, organochlorines (OC) are widely used in the pharmaceutical industry, in plastics, and in the manufacture of pesticides, among other applications. These compounds and the byproducts of their decomposition deserve attention and efficient proposals for their treatment. Among sustainable alternatives, the use of ligninolytic enzymes (LEs) from fungi stands out, as these molecules can catalyze the transformation of a wide range of pollutants. Among LEs, laccases (Lac) are known for their efficiency as biocatalysts in the conversion of organic pollutants. Their application in biotechnological processes is possible, but the enzymes are often unstable and difficult to recover after use, driving up costs. Immobilization of enzymes on a matrix (support or solid carrier) allows recovery and stabilization of this catalytic capacity. Agricultural residual biomass is a passive environmental asset. Although underestimated and still treated as an undesirable component, residual biomass can be used as a low-cost adsorbent and as a support for the immobilization of enzymes. In this review, the adsorption capacity and immobilization of fungal Lacon supports made from residual biomass, including compounds such as biochar, for the removal of OC compounds are analyzed and compared with the use of synthetic supports. A qualitative and quantitative comparison of the reported results was made. In this context, the use of peanut shells is highlighted in view of the increasing peanut production worldwide. The linkage of methods with circular economy approaches that can be applied in practice is discussed.

Characteristics of the modern nut market

Despite seasonal fl uctuations depending on annual climatic conditions, the nut market is showing steady growth. The increase in nut production is explained by the rise in their consumption as a raw material for confectionery products, as well as for direct consumption as an alternative source of basic and physiologically active substances. In total, 5,367,530 tons of nuts were produced in the 2022–2023 marketing year (excluding peanuts). Almonds lead the world production. It is followed by walnuts, cashews, pistachios, and hazelnuts. Pine nuts are the only nut produced in the Russian Federation on an industrial scale. According to Russian data, in the 2022/23 season, the country produced about 35 thousand tons of pine nuts in shell, which is half of the world production of this type. Global production of inshell peanuts annually is about 50 million tons.

Long-Term Maize Intercropping with Peanut and Phosphorus Application Maintains Sustainable Farmland Productivity by Improving Soil Aggregate Stability and P Availability

The intercropping of maize (Zea mays L.) and peanuts (Arachis hypogaea L.) (M||P) significantly enhances crop yield. In a long-term M||P field experiment with two P fertilizer levels, we examined how long-term M||P affects topsoil aggregate fractions and stability, organic carbon (SOC), available phosphorus (AP), and total phosphorus (TP) in each aggregate fraction, along with crop yields. Compared to their respective monocultures, long-term M||P substantially increased the proportion of topsoil mechanical macroaggregates (7.6–16.3%) and water-stable macroaggregates (>1 mm) (13.8–36.1%), while reducing the unstable aggregate index (ELT) and the percentage of aggregation destruction (PAD). M||P significantly boosted the concentration (12.9–39.9%) and contribution rate (4.1–47.9%) of SOC in macroaggregates compared to single crops. Moreover, the concentration of TP in macroaggregates (>1 mm) and AP in each aggregate fraction of M||P exceeded that of the respective single crops (p < 0.05). Furthermore, M||P significantly increased the Ca2-P, Ca8-P, Al-P, and Fe-P concentrations of intercropped maize (IM) and the Ca8-P, O-P, and Ca10-P concentrations of intercropped peanuts (IP). The land equivalent ratio (LER) of M||P was higher than one, and M||P stubble improved the yield of subsequent winter wheat (Triticum aestivum L.) compared with sole-crop maize stubble. P application augmented the concentration of SOC, TP, and AP in macroaggregates, resulting in improved crop yields. In conclusion, our findings suggest that long-term M||P combined with P application sustains farmland productivity in the North China Plain by increasing SOC and macroaggregate fractions, improving aggregate stability, and enhancing soil P availability.

Characterization of gene expression patterns in response to an orthotospovirus infection between two diploid peanut species and their hybrid.

Tomato spotted wilt orthotospovirus (TSWV) transmitted by thrips causes significant yield loss in peanut (Arachis hypogaea L.) production. Use of peanut cultivars with moderate field resistance has been critical for TSWV management. However, current TSWV resistance is often not adequate, and the availability of sources of tetraploid resistance to TSWV is very limited. Allotetraploids derived by crossing wild diploid species could help introgress alleles that confer TSWV resistance into cultivated peanut. Thrips-mediated TSWV screening identified two diploids and their allotetraploid possessing the AA, BB, and AABB genomes Arachis stenosperma V10309, Arachis valida GK30011, and [A. stenosperma × A. valida]4x (ValSten1), respectively. These genotypes had reduced TSWV infection and accumulation in comparison with peanut of pure cultivated pedigree. Transcriptomes from TSWV-infected and non-infected samples from A. stenosperma, A. valida, and ValSten1 were assembled, and differentially expressed genes (DEGs) following TSWV infection were assessed. There were 3,196, 8,380, and 1,312 significant DEGs in A. stenosperma, A. valida, and ValSten1, respectively. A higher proportion of genes decreased in expression following TSWV infection for A. stenosperma and ValSten1, whereas a higher proportion of genes increased in expression following infection in A. valida. The number of DEGs previously annotated as defense-related in relation to abiotic and biotic stress was highest in A. valida followed by ValSten1 and A. stenosperma. Plant phytohormone and photosynthesis genes also were differentially expressed in greater numbers in A. valida followed by ValSten1 and A. stenosperma, with over half of those exhibiting decreases in expression.

Produtividade da cultura do amendoim sob irrigação plena e deficitária na fase reprodutiva

ABSTRACT Peanut cultivation has national and global significance in agriculture and industry. Under water-limited conditions, its yield decreases to the extent that it compromises the success of the exploitation. This study focused on evaluating peanut crop yield and production components under full and deficit irrigation applied in the reproductive phase, to determine the impact of water supply on the yield of peanuts grown in the off-season in the State of São Paulo, Brazil. The experiment was conducted for two years and followed a randomized blocks design with five irrigation levels distributed in strips, with four repetitions. The treatments consisted of crop evapotranspiration replenishments from 100 to 10%. In the first year, under suitable temperatures, the maximum yield reached 3,922 kg ha-1 with the application of 277 mm of irrigation depth and decreased up to 35% with the lowest irrigation depth (48 mm). In the second year, suboptimal temperatures caused a delay in the growing cycle and caused a 33% reduction in crop yields. Despite the potential benefits of deficit irrigation in increasing water productivity, low temperatures pose a risk to peanut yield, especially during the off-season crop in the region.

Response of Diverse Peanut Cultivars to Calcium Fertilization under Clay Soil Conditions

The absence of calcium decreases the production of peanuts compared to any other element. To investigate the influence of calcium (Ca) applications on the production and seed quality of four diverse peanut cultivars from different regions—USA (NC-7), Egypt (Giza-6) and Nigeria (Samnut-23 and Samnut-24)—under clay soil conditions, two experiments were carried out at the Agricultural Experimental and Research Station, Cairo University, Egypt, during the two seasons of 2016 and 2017. The experimental design was a randomized complete block in a spilt-plot arrangement with three replications. The main plots were allocated to four peanut cultivars (Giza-6, Samnut-23, Samnut-24 and NC-7), and the sub-plots were devoted to calcium applications (soil application in the form of calcium sulfate dihydrate, foliar application in the form of calcium oxide and the control treatment of distilled water). Results indicated that all four peanut cultivars responded differently to the application of calcium fertilizers. The calcium application significantly enhanced peanut growth, yield components, biological, pod, seed, oil yields, seed oil, free fatty acids and seed calcium percentages in different cultivars. Soil calcium application significantly improved peanut production compared to foliar calcium application. NC-7 cultivar treated with the soil Ca application resulted in the maximum values of biological yield (92.9-ton ha−1), pod yield (6.8-ton ha−1), seed yield (4.4-ton ha−1), oil yield (2247.0 kg ha−1), pod index (203.2 g) and seed index (84.1 g). The interaction between the NC-7 cultivar and soil calcium applications is recommended to attain the best combination, leading to the highest yield and seed quality of peanuts.

Kaolinite reduced Cd accumulation in peanut and remediate soil contaminated with both microplastics and cadmium

Microplastics (MPs) increase the effective state of heavy metals (HMs) in soil and seriously threaten the yield and quality of peanuts (Arachis Hypogea L.). Kaolinite (KL) has the potential to ameliorate MP- and HM- contaminated soils, but the mechanism of action between them is not well understood. Therefore, 60-day experiments were conducted, where KL (1 %, 2 %) and MPs (0.1 %, 1 %) were individually or jointly mixed into soils with different cadmium (Cd) concentrations (0.5, 2.5, and 5.0 mg·kg−1) to cultivate peanuts in a greenhouse. Finally, soil-bioavailable Cd, peanut dry weight, peanut Cd concentrations, the pH, cation exchange capacity (CEC), dissolved organic carbon (DOC), microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) were determined. It was shown that MPs negatively affected the peanut dry weight and increased the content of soil-bioavailable Cd and Cd concentration in peanut. In the MP- and Cd-contaminated soils, KL mitigated the negative influence of MPs by increasing the dry weight of peanuts by 8.40 %−40.59 %, decreasing the soil-bioavailable Cd by 23.70–35.74 %, and significantly decreasing peanut Cd concentrations by 9.65–30.86 %. The presence of MPs decreased soil pH (7.69–7.87) and the CEC (20.96–23.95 cmol·L−1) and increased the soil DOC (1.84–2.26 mg·kg−1). KL significantly increased soil pH (7.79–8.03) and the CEC (24.96–28.28 cmol·L−1) and mitigated the adverse influence of MPs on the pH and CEC of Cd-contaminated soils. A regression path analysis (RPA) evidenced that KL decreased Cd accumulation in plants by changing the properties of soil contaminated with MPs and Cd. The research results revealed the mechanism of KL on peanut growth and Cd absorption in MP- and Cd-contaminated soil. The results of this study provide a foundation to improve the quality of MP- and HM-contaminated soils and realize safe peanut production.

Effect of Drought and Pluvial Climates on the Production and Stability of Different Types of Peanut Cultivars in Guangdong, China

The production and breeding of peanuts was restricted by the frequently extreme climatic conditions in Guangdong province, China. To understand the influence of drought and pluvial climates on peanut traits and yield, a phenotypic investigation of seventy peanut cultivars was conducted from 2018 to 2022; comprehensive field meteorological data collection, and typical drought (2021) and pluvial (2022) climates were recorded. The results revealed that the cultivars achieved the highest single plant pod weight (SPPW) and single plant seed weight (SPSW) of 61.03 g and 45.84 g, respectively, in drought conditions, followed by the control, and finally the pluvial. The SPPW, SPSW and eight agronomy traits exhibited significant differences across the different climatic conditions. Correlation analysis revealed the yield traits and key yield-related traits were positively or negatively correlated with soil water content (SWC), total global radiation (TGR), total precipitation (TP) and total net radiation (TNR). The intermediate and Spanish type cultivars were more stable and productive than the other botanical types of cultivars, commercial varieties exhibited better performance than landraces, and seven cultivars were identified with good production potential, under drought and pluvial conditions. Our study showed that pluvial climate was detrimental to peanut yield, and the SPPW and SPSW were significantly influenced by climates with genotype differences.

The combined effect of biochar and organic fertilizer (goat manure) on the growth and yield of peanut crop (Arachis hypogaea L.)

Sandy soils suffer from many problems with physical and chemical properties as well as low productivity of cultivated crops. Biochar and organic fertilizer have been used in agriculture to improve soil fertility, enhance crop productivity, and sequester carbon in the soil. A study was conducted to investigate the effect of biochar (B) from woody materials and gout manure (GM) on the growth and yield of peanut crops (Arachis hypogaea L.) in sandy soil. The experiment was in a randomized complete block design RCBD with three replicates. The treatments were controlled, 10 t ha-1 GM, 2 % B + 10 t ha-1 GM, and 5 % B + 10 t ha-1 GM. The result showed that the addition of 2 % B + 10 t ha-1 GM increased peanut yield by 50 % while the addition of 5 % B + GM 10 t ha-1 increased peanut yield by 82 %. Without biochar, goat manure had yield increases by 25 % compared to a control. Overall, our findings demonstrate that biochar-goat manure combinations have a better performance than GM alone, in terms of yield and plant growth. For future research, we tend to determine the optimal combination of biochar and goat manure for peanut production.

PERTUMBUHAN DAN PRODUKSI KACANG TANAH (Arachis hypogaea L.) PADA BERBAGAI DOSIS KALIUM ORGANIK

Potassium is a macro nutrient needed for plant growth, especially for the formation of pods and seeds. Organic potassium sources such as coconut fiber ash can be used as an alternative to reduce the use of chemical fertilizers. Cow urine contains nitrogen nutrients which can be used as a source of N nutrients to reduce the use of urea fertilizer. This research aims to determine the dose of organic potassium, the dose of liquid organic fertilizer (LOF) from cow urine on the growth and production of peanuts. This research was structured using a Randomized Block Design (RBD) with 2 treatments, namely doses of organic potassium and cow urine liquid organic fertilizer (LOF). The parameters observed were plant height (cm), number of leaf stalks, seed weight per 1000 (gr), number of pods (seeds), fresh weight (gr), and dry weight (gr). The results of the study showed that treatment with doses of organic potassium and cow urine LOF had no significant effect on the growth and production of peanuts. The best dose of organic potassium is organic potassium equivalent to a dose of 75 kg KCl/ha. The best dose of cow urine LOF is 75 ml/l water (U2). The best combination of potassium dose equivalent to 75 Kg KCl/ha with a LOF dose of cow urine for growth and production of peanuts LOF cow urine 50 ml/l (A2U1).

Sosialisasi Pembuatan Yoghurt Kacang Tanah sebagai Pemberdayaan Masyarakat untuk Peningkatan Ekonomi Di Desa Landungsari Kecamatan Dau Kabupaten Malang

The increase in public awareness of health during the Covid 19 pandemic was accompanied by an increasing need for functional food including probiotic drinks. One of the efforts to diversify yogurt is by using peanuts as a substitute for cow's milk. The production of peanuts which is quite a lot in Malang allows it to be processed into Plant-based yogurt. As an effort to increase independence in the field of entrepreneurship in peanut-producing area in Desa Landungsari Kecamatan Dau Malang, it was necessary to provide socialization on making peanut yogurt and business development through the digital economy by the program team serving Qaryah Thayyibah UIN Maulana Maliki Ibrahim Malang.

Impact of Variety and At-Planting Insecticides on Thrips (Thysanoptera: Thripidae) Management in Peanut

Tobacco thrips are one of the most prevalent early season insect pests in peanut production systems. Experiments were conducted in 2018 and 2019 in Stoneville Mississippi to evaluate integrated pest management approaches to mitigate negative effects of thrips. Experimental treatments included a normal and late planting date, varieties Georgia-06G and QR-14, and in-furrow insecticide treatments consisting of a non-treated control, phorate, and imidacloprid. Measurements included early and final plant stands, immature and adults thrips counts, and pod yield. In-furrow insecticide had a strong effect on number of thrips per plant with treatment thrips ranking of: phorate < imidacloprid < non-treated control. However, in-furrow insecticide had no interaction with variety or plating date. Although phorate provided greater thrips control than imidacloprid, pod yield for the two treatments was similar, and both in-furrow treatments had greater yield than the non-treated control. The variety QR-14 had greater pod yield than Georgia-06G when planted late. Overall, these results confirm that in-furrow insecticide applications of either imidacloprid or phorate are adequate in protecting against yield loss from thrips within a midsouthern peanut production environment.

Performance of Imidacloprid or Phorate Co-Applied In-Furrow with Superabsorbent Polymer and Evaluation of Aldicarb

Management decisions mitigating thrips injury and tomato spotted wilt infection are primarily limited to being enacted at the beginning of the growing season. Previous work explored corresponding potential management contribution of concurrent insecticide application with a superabsorbent polymer in-furrow but reported effects to be marginal or variable across experiments. To more thoroughly probe this potential, data from a collection of 40 experiments conducted from 2009 through 2022 was analyzed to examine the efficacy of insecticides (aldicarb, imidacloprid, imidacloprid plus fluopyram, and phorate, in addition to a nontreated check) applied in-furrow at planting for peanut production. In addition to treatments applied individually, experiments included imidacloprid (n = 11) or phorate (n = 18) applied in the presence of 2.2 kg/ha superabsorbent polymer. Results indicated that neither imidacloprid nor phorate alone significantly varied with regard to stand count (P > 0.35), thrips injury (P > 0.27), tomato spotted wilt incidence (P > 0.08), or yield (P > 0.37) when compared to their co-application with the polymer. These results were consistent across variety susceptibility levels to tomato spotted wilt. The fitted models were determined to be robust to the treatment composition of screened studies (P > 0.089). Where there were differences in projected returns above the nontreated check, phorate or aldicarb were the most consistently profitable treatments.

Managing Risk: a Decision Tool to Enhance Yield and Quality of Peanut (Arachis hypogaea) in Ghana

Peanut cultivation in Ghana is influenced by abiotic (e.g., drought, fertilization, etc.) and biotic (e.g., pests, diseases, etc.) factors that must be managed effectively to maximize yields. These factors differ across agro-ecologies and pose significant risks to yields and aflatoxin contamination and can limit financial returns to farmers. Knowledge of interactions among key production practices such as pest management, crop rotation sequence, varietal selection, tillage systems, and chemical inputs is required to mitigate these risks and to provide quality advice to peanut farmers by Agricultural Extension Agents (AEAs) and/or similar advisory groups. This paper describes the development of a comprehensive, Microsoft (MS) Excel based risk management tool to help farmers and their advisors implement practices that minimize the risk of yield loss and aflatoxin contamination while providing production cost estimates. This tool is based on a similar one developed for peanut production in North Carolina (USA). Three risk tools were developed; one for the interior savannah zone of northern Ghana which has a unimodal rainfall pattern, and two for the forest and transition zones of southern Ghana which are characterized by a bimodal rainfall pattern. These tools will aid AEAs and farmers to make informed decisions on the best combinations of production practices that will minimize risk and increase yields in Ghana. The development process considered the differences in selection of varieties, plant density, fertilization, and pest management between savannah and the forest-transition regions as well as the impact of their interactions on peanut yield and aflatoxin contamination. Overall, this risk management tool benefits extension service providers (government and private), breeding, agronomy, and plant protection programs as well as teaching in higher education institutions.

Arbuscular Mycorrhizal Fungi Restored the Saline-Alkali Soil and Promoted the Growth of Peanut Roots.

Peanut (Arachis hypogaea L.) is an important oil and cash crop. An efficient utilization of saline-alkali soil resources, the development of peanut planting in saline-alkali soil, and obtaining high and stable yield have become urgent needs to ensure peanut production. Arbuscular mycorrhizal fungi (AMF) have been reported to develop the potential productivity of host plants and improve their salt resistance and tolerance. However, there is still limited research on promoting the growth and morphology of peanut roots. Therefore, in this study, seeds of salt-tolerant peanut variety "HY 25" were coated with commercial AMF inoculant before being planted in saline-alkali and normal soils to investigate the effects of AMF on peanut root growth and rhizosphere soil. The results showed that root morphological characteristics were significantly increased by the use of AMF at the podding stage in saline-alkali soil and from the flowering and pegging stage to the maturity stage in normal soil. Of note, the total root volume of peanut inoculated with AMF significantly increased by 31.57% during the podding stage in saline-alkali soil. Meanwhile, AMF significantly increased the phosphatase and invertase activities in the peanut rhizosphere of saline-alkali soil from the flowering stage to maturity stage and soil CAT activity at the maturity stage (41.16~48.82%). In normal soil, soil phosphatase and urease activities were enhanced by AMF at the flowering stage and the podding stage, respectively. AMF also increased the contents of soil organic matter, available phosphorus, and hydrolysable nitrogen, but decreased soil EC in saline-alkali soil. In addition to the significant increase in soil available phosphorus content, AMF had no significant effect on the physical and chemical properties of the soil and other soil nutrients in normal soil. AMF significantly increased pod biomass and yield in saline-alkali soil and normal soil, and improved their agronomic characteristics. In conclusion, peanut seeds coated with AMF improved the root morphological characteristics of peanuts and improved the physical and chemical properties in peanut rhizosphere, especially in saline-alkali soil. The process of rhizosphere soil nutrient transformation was also enhanced. Finally, AMF improved plant agronomic traits to increase the pod yield (16.5~21.9%). This study provides the theoretical basis and technical support for the application of AMF in peanut production in saline-alkali soil.

Seasonal differences in yield and fertilizer use efficiency of different low-calcium-tolerant peanut varieties in response to the timing and splitting of calcium application in southern China

Soil Ca deficiency hinders the development of the peanut industry, and inappropriate Ca fertilizer application increases the risk of soil environmental degradation and reduces the fertilizer utilization rate and yield in peanuts. N fertilizer doses are frequently split and applied over different time periods. However, whether this widespread method is applicable to Ca fertilizer and improves peanut growth and yield in southern China requires further investigation. Thus, this study evaluated the effects of timing and splitting Ca applications on the dry matter accumulation and partition, fertilizer use efficiency, and yield of different low-Ca-tolerant peanut varieties (low-Ca-sensitive Jihua 16 and low-Ca-tolerant Yueyou 45) in the early and late seasons. The results showed that the early timing and splitting of Ca application significantly increased the pod yield of Jihua 16 by increasing nitrogen and calcium use efficiencies, dry matter accumulation, and assimilate transfer from vegetative parts to the pods in both years. The highest pod yield of Jihua 16 was obtained with 300 kg CaO ha−1 basal application before sowing and 300 kg CaO ha−1 top-dressing application at the flowering stage (B300:T300) in the early season and 600 kg CaO ha−1 basal application before sowing and 600 kg CaO ha−1 top-dressing application at the flowering stage (B600:T600) in the late season; the yields were significantly increased by 91.87 % in 2020, 92.15 % in 2021, and by 255.36 % in 2020, 248.22 % in 2021, respectively, compared with those obtained with no Ca fertilizer use. However, the Ca treatments did not significantly affect pod yield or the related parameters of Yueyou 45 in either season. The pod yield of both cultivars was higher in the early season than that in the late season because of the higher effective accumulated temperature and precipitation during the reproductive stage in the early season of both years. The results of this study indicate that the early season is most suitable for peanut production, and the B300:T300 and no Ca application strategies are appropriate for the Ca-sensitive and -tolerant cultivars, respectively, in the early season in southern China. Notably, additional Ca-tolerant cultivars should be selected for cultivation in southern China to reduce future fertilizer application. These results provide useful information for improving double-cropping systems, Ca fertilization strategies, and peanut cultivar breeding in southern China.

A study on creating highly yielded and drought-tolerant QTL gene-possessed lines of peanut

In the trend of global climate changes and heavily occurring drought, particularly resulting in decreased productivity and cultivated areas of peanut production, the breeding and screening of highly yielded and drought-tolerant QTL gene-possessed peanut lines is considered a strategic and effective solution. In this study, the on-field assessment in combination with drought-tolerant gene screening was implemented and 6 peanut cultivars named TK10, Chum Nghe An, Sen Nghe An, L20, L23, and L14 were regarded as hybrid parents for the crossing-breeding program. Of 5 cross-breeding combinations (Chum Nghe An played the role of mother; 5 others were the fathers) 15 elite peanut lines of the F3 generation with high yield (over 29 quintals/ha) and 5 of them coded F3-6, F3-12 (Chum Nghe An × L23), F3-9, F3-10 (Chum Nghe An × Sen Nghe An), and F3-13 (Chum Nghe An × L14) possessed the locus for drought tolerance determined by electrophoresis technology. It is also particularly mentioned that all these lines had a good recovery (92.8-94.6, 84.4-88.4, and 80.0-83.4%, respectively), average productivity loss (30.56-34.10%), and good drought tolerance (point 3: G=21-40%).

Paper-based microfluidic device for selective detection of peanut allergen Ara h1 applying black phosphorus-Au nanocomposites for signal amplification

This paper developed a portable microfluidic paper-based analysis device (μ-PAD) combined with the electrochemical technique for efficient and sensitive detection of peanut allergen Ara h1. The proposed μ-PAD works based on the variation of differential pulse voltammetry (DPV) response current induced by peanut allergen Ara h1. Black phosphorus (BP)–Au nanocomposites were introduced both to improve the electron transfer rate at the electrode interface for signal amplification, and to immobilize the specific Ara h1 aptamers through Au–S bonding to recognize the target in food matrices. This μ-PAD had good specificity and detection stability for Ara h1 allergen and could complete the entire analysis process within 20 min, achieving a wide linear response range (25–800 ng mL−1) and a low detection limit (LOD, 11.8 ng mL−1). In the Ara h1 allergen detection applied to real peanut products (cookies, milk, and bread), the constructed μ-PAD obtained acceptable recoveries (93.50%–101.86%) with relative standard deviations (RSDs) of 0.36–2.97% (n = 3), with a good correlation with the ELISA results (R2 = 0.9956). Therefore, the portable μ-PAD based on BP-Au nanocomposites was demonstrated to provide an effective strategy for rapid analysis and screening of Ara h1 allergen in food, which has broad application prospects.

Development of a Greenhouse Method to Evaluate the Peanut Resistance to Athelia rolfsii

Peanut stem rot (also known as white mold), caused by the fungus Athelia rolfsii, is one of the most damaging soilborne pathogens for U.S. peanut production. The disease is mainly controlled by fungicides and by adopting cultivars with moderate resistance. Field evaluation is the main approach for evaluating plant resistance, but it is costly and labor-intensive. Reliable methods for in vitro or greenhouse evaluations are desirable. Greenhouse, growth chamber, and in vitro methods have been tried to assess resistance, but they generally do not correlate well with field results. In this study, we developed a reliable method to assess resistance to stem rot on peanut under greenhouse conditions. Stem cuttings were taken from 60-day-old plants and placed into a cup filled with potting mix. Cuttings were inoculated with active A. rolfsii mycelial plugs above the soil line, with mycelium directly contacting the stem. Inoculated cuttings were put in a mist chamber to keep humidity high. Highly significant differences in disease resistance among genotypes were found at 7 days after inoculation ( P < 0.05). Moreover, evaluations done at 3, 5, 7, and 9 days after inoculation and the area under the disease progress curve were significantly correlated with field evaluations of the same genotypes. This greenhouse assay is a step forward to screen peanut germplasm more efficiently for stem rot resistance. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

Whole genome analysis of Bacillus amyloliquefaciens TA-1, a promising biocontrol agent against Cercospora arachidicola pathogen of early leaf spot in Arachis hypogaea L

BackgroundEarly leaf spot disease, caused by Cercospora arachidicola, is a devastating peanut disease that has severely impacted peanut production and quality. Chemical fungicides pollute the environment; however, Bacillus bacteria can be used as an environmentally friendly alternative to chemical fungicides. To understand the novel bacterial strain and unravel its molecular mechanism, De novo whole-genome sequencing emerges as a rapid and efficient omics approach.ResultsIn the current study, we identified an antagonistic strain, Bacillus amyloliquefaciens TA-1. In-vitro assay showed that the TA-1 strain was a strong antagonist against C. arachidicola, with an inhibition zone of 88.9 mm. In a greenhouse assay, results showed that the TA-1 strain had a significant biocontrol effect of 95% on peanut early leaf spot disease. De novo whole-genome sequencing analysis, shows that strain TA-1 has a single circular chromosome with 4172 protein-coding genes and a 45.91% guanine and cytosine (GC) content. Gene function was annotated using non-redundant proteins from the National Center for Biotechnology Information (NCBI), Swiss-Prot, the Kyoto Encyclopedia of Genes and Genomes (KEGG), clusters of orthologous groups of proteins, gene ontology, pathogen-host interactions, and carbohydrate-active enZYmes. antiSMASH analysis predicted that strain TA-1 can produce the secondary metabolites siderophore, tailcyclized peptide, myxochelin, bacillibactin, paenibactin, myxochelin, griseobactin, benarthin, tailcyclized, and samylocyclicin.ConclusionThe strain TA-1 had a significant biological control effect against peanut early leaf spot disease in-vitro and in greenhouse assays. Whole genome analysis revealed that, TA-1 strain belongs to B. amyloliquefaciens and could produce the antifungal secondary metabolites.