Peanut Crop Research Articles

Peanut Plant Development Strategy as a Leading Commodity in South Tinangkung District, Banggai Islands Regency

Agricultural development aims to improve the living standards of farmers and the welfare of their people. The prospects for the development of peanut farming business are very bright in order to increase the income and welfare of farmers, so there is a need for a strategy in the development of peanuts as a leading commodity. Thus, this study aims to develop a strategy for developing peanuts as a leading commodity in South Tinangkung District, Banggai Islands Regency. The research was conducted from June to November 2022 in South Tinangkung District, Banggai Islands Regency. The data used in this study is primary data. Primary data were obtained by conducting structured interviews and direct observations from certain natural places by circulating questionnaires to respondents. Furthermore, the research used the SWOT method, namely SO (Strength-Opportunity), WO (Weakness-Opportunity), ST (Strength-Threats), and WT (Weakness-Threats). The results obtained in this study are that the development of peanut crop commodities in South Tinangkung District is in the second tier (diversification). so that the most appropriate strategy to be applied is Strategy-ST, thus the main strategy for the development of peanuts as a superior commodity is to utilize the experience of farmers in farming peanut crops with the ability to carry out land management to overcome the shortage of Agriculture machinery needs, in addition to that, it can maximize the role of Agricultural cooperation and can reduce high labor wages.

Genome-Wide Investigation of Apyrase (APY) Genes in Peanut (Arachis hypogaea L.) and Functional Characterization of a Pod-Abundant Expression Promoter AhAPY2-1p

Peanut (Arachis hypogaea L.) is an important food and feed crop worldwide and is affected by various biotic and abiotic stresses. The cellular ATP levels decrease significantly during stress as ATP molecules move to extracellular spaces, resulting in increased ROS production and cell apoptosis. Apyrases (APYs) are the nucleoside phosphatase (NPTs) superfamily members and play an important role in regulating cellular ATP levels under stress. We identified 17 APY homologs in A. hypogaea (AhAPYs), and their phylogenetic relationships, conserved motifs, putative miRNAs targeting different AhAPYs, cis-regulatory elements, etc., were studied in detail. The transcriptome expression data were used to observe the expression patterns in different tissues and under stress conditions. We found that the AhAPY2-1 gene showed abundant expression in the pericarp. As the pericarp is a key defense organ against environmental stress and promoters are the key elements regulating gene expression, we functionally characterized the AhAPY2-1 promoter for its possible use in future breeding programs. The functional characterization of AhAPY2-1P in transgenic Arabidopsis plants showed that it effectively regulated GUS gene expression in the pericarp. GUS expression was also detected in flowers of transgenic Arabidopsis plants. Overall, these results strongly suggest that APYs are an important future research subject for peanut and other crops, and AhPAY2-1P can be used to drive the resistance-related genes in a pericarp-specific manner to enhance the defensive abilities of the pericarp.

Promotion of Peanut (Arachis hypogaea L.) Growth by Plant Growth-Promoting Microorganisms

Brazil is an important peanut producer, but despite its high production, there still needs to be an inoculant for the peanut crop. In addition, the use of microorganisms that promote plant growth (PGPM) is not common, and this crop is highly dependent on chemical fertilizers. An excellent alternative to reduce the use of fertilizers and chemical inputs in peanut crops while reducing the production cost and environmental impact is the use of PGPM. The objective of this study was to evaluate the effects of Azospirillum brasilense, Bacillus subtilis, Bradyrhizobium japonicum, and Trichoderma harzianum as single inoculants and co-inoculants on the growth promotion and productivity of peanuts in greenhouse and field conditions. In the greenhouse, the experiment was conducted with 12 treatments with six repetitions. In the field conditions, the experiment was conducted with five treatments with four repetitions. Both experiments were conducted in randomized blocks. In general, all the microorganisms evaluated in the present study promoted increases in root dry mass, shoot dry mass, phosphorus concentrations, and plant height in the greenhouse and under field conditions compared with the control. Interestingly, the mixtures of microorganisms inoculated in peanut plants did not promote greater plant growth and development compared with inoculations of the microorganisms separately. Specifically, in the field, the highest productivity was found for the inoculation of B. japonicum alone. The PGPM evaluated in the present study for peanut crops generally promoted some increases in productivity in greenhouse and field conditions.

Inventarisasi Gulma Pada Pertanaman Kacang Tanah (Arachis hypogaea L.) Di Kabupaten Ogan Komering Ilir Sumatera Selatan

Research on the inventory of weeds on peanuts crops (Arachis hypogaea L.) in the village of Tanjung Merindu sub district Tanjung Lubuk Ogan Komering Ilir, has been carried out paper March to April 2019. This study aims to determine what kind of weed found in peanut crop (Arachis hypogaea L.) in the village of Tanjung Merindu sub district Tanjung Lubuk Ogan Komering Ilir. The research used descriptive survey method with exploring technique. Weeds that can be made key determination, identification, and made herbarium. Result of research got 2 Division, 3 Class, 7 Ordo, 7 Familia, 9 Genus, and 9 Species weeds are: Cyperus rotundus L., Axonopus compressus Swartz., Imperata cylindrica L., Brachiaria mutica Forsk., Euphorbia hirta L., Ageratum conyzoides L., Mimosa pudica Duchass., Cleome rutidosperma Backer., dan Neprholepis exaltata Schott. Keywords: Inventory, Weeds, Peanuts.Â

Enterobacter sp. J49: A Native Plant Growth-Promoting Bacteria as Alternative to the Application of Chemical Fertilizers on Peanut and Maize Crops

In agricultural soils the productivity is determined by several factors and among them are the metabolic activities of the microorganisms that reside in it. The inoculation of plants with these bacteria is an alternative to the use of agrochemicals in crops. In particular, in those soils in which P levels are low, phosphate-solubilizing bacteria became an important group of soil microorganisms. In order to propose a potential P-biofertilizer to replace chemical fertilizers, the objective of this study was to evaluate the response of peanut and maize plants to the inoculation with the phosphate solubilizer Enterobacter sp. J49 individually or in combination with chemical fertilizers on growth, yield, and nutrient contents on peanut and maize plants in field trials. Two field assays in the peanut growing region of Córdoba Province (Argentina) were carried out. The inoculation of peanut with Enterobacter sp. J49 showed an increase in the yield with respect to the other treatments. Maize plants inoculated with this strain, alone or combined with half dose of chemical fertilizer, presented the highest yields. The results indicated that Enterobacter sp. J49 has a growth-promoting effect on the yield of peanut and maize mainly under drought stress. In conclusion, the inoculation with this strain would be a more sustainable agricultural practice for improving yield of peanut and maize crops in Argentinian agricultural area.

Negative effects on the development of Chrysodeixis includens and Spodoptera cosmioides fed by peanut plants inoculated with entomopathogenic fungi.

Recent studies have shown that entomopathogenic fungi, as endophytes, can have beneficial effects on plants, protecting them from defoliating insects. The potential of endophytic association by entomopathogenic fungi with the peanut crop has been little explored. In our study, we conducted experiments by inoculation of peanut seeds through a soil drench method with nine strains/species of entomopathogenic fungi of the genera Metarhizium, Beauveria and Cordyceps, subsequently these plants were consumed by two larval pests, Chrysodeixis includens and Spodoptera cosmioides. The parameters of larval growth rates, mortality, foliar consumption and larval period were observed during the development of larvae. In addition, the endophytic capacity of these fungi in peanut plants and their persistence in soil were investigated. In two replicate greenhouse trials for each larva, peanut plants were inoculated with fungi by the soil-drench method. We evaluated the performance of C. includens and S. cosmioides feeding on inoculated peanut plants starting at the 2nd larval instar. The larval and pupal weights of C. includens and S. cosmioides were significantly different among the fungal treatment groups, where insects feeding on control plants exhibited higher larval and pupal weights than insects feeding on treated plants. The differences in larval period showed that Control larvae pupated faster than the larvae fed on fungal-inoculated plants, fungal treatments had a larval period of 3 to 5 days more than the control. The mortality rates of C. includens and S. cosmioides were significantly different among the fungal treatment groups, insects fed on Control plants exhibited higher survival than insects fed on fungal-inoculated plants. The persistence of all Metarhizium fungi was higher in the soil compared to other fungi, and only Metarhizium and B. bassiana IBCB215 emerged from the phyllosphere of peanut plants. Although the fungus Cordyceps presented the worst performance among the fungal treatments. Overall, our results demonstrate the negative effects on the development of C. includens and S. cosmioides that were fed on fungal-inoculated peanut plants, the best results recorded were for Metarhizium strains and the fungus B. bassiana IBCB215.

Genome-wide characterization of phospholipase D family genes in allotetraploid peanut and its diploid progenitors revealed their crucial roles in growth and abiotic stress responses.

Abiotic stresses such as cold, drought and salinity are the key environmental factors that limit the yield and quality of oil crop peanut. Phospholipase Ds (PLDs) are crucial hydrolyzing enzymes involved in lipid mediated signaling and have valuable functions in plant growth, development and stress tolerance. Here, 22, 22 and 46 PLD genes were identified in Arachis duranensis, Arachis ipaensis and Arachis hypogaea, respectively, and divided into α, β, γ, δ, ε, ζ and φ isoforms. Phylogenetic relationships, structural domains and molecular evolution proved the conservation of PLDs between allotetraploid peanut and its diploid progenitors. Almost each A. hypogaea PLD except for AhPLDα6B had a corresponding homolog in A. duranensis and A. ipaensis genomes. The expansion of Arachis PLD gene families were mainly attributed to segmental and tandem duplications under strong purifying selection. Functionally, the most proteins interacting with AhPLDs were crucial components of lipid metabolic pathways, in which ahy-miR3510, ahy-miR3513-3p and ahy-miR3516 might be hub regulators. Furthermore, plenty of cis-regulatory elements involved in plant growth and development, hormones and stress responses were identified. The tissue-specific transcription profiling revealed the broad and unique expression patterns of AhPLDs in various developmental stages. The qRT-PCR analysis indicated that most AhPLDs could be induced by specific or multiple abiotic stresses. Especially, AhPLDα3A, AhPLDα5A, AhPLDβ1A, AhPLDβ2A and AhPLDδ4A were highly up-regulated under all three abiotic stresses, whereas AhPLDα9A was neither expressed in 22 peanut tissues nor induced by any abiotic stresses. This genome-wide study provides a systematic analysis of the Arachis PLD gene families and valuable information for further functional study of candidate AhPLDs in peanut growth and abiotic stress responses.

Genome-wide identification, evolutionary and expression analysis of the cyclin-dependent kinase gene family in peanut

BackgroundCyclin-dependent kinases (CDKs) are a predominant group of serine/threonine protein kinases that have multi-faceted functions in eukaryotes. The plant CDK members have well-known roles in cell cycle progression, transcriptional regulation, DNA repair, abiotic stress and defense responses, making them promising targets for developing stress adaptable high-yielding crops. There is relatively sparse information available on the CDK family genes of cultivated oilseed crop peanut and its diploid progenitors.ResultsWe have identified 52 putative cyclin-dependent kinases (CDKs) and CDK-like (CDKLs) genes in Arachis hypogaea (cultivated peanut) and total 26 genes in each diploid parent of cultivated peanut (Arachis duranensis and Arachis ipaensis). Both CDK and CDKL genes were classified into eight groups based on their cyclin binding motifs and their phylogenetic relationship with Arabidopsis counterparts. Genes in the same subgroup displayed similar exon–intron structure and conserved motifs. Further, gene duplication analysis suggested that segmental duplication events played major roles in the expansion and evolution of CDK and CDKL genes in cultivated peanuts. Identification of diverse cis-acting response elements in CDK and CDKL genes promoter indicated their potential fundamental roles in multiple biological processes. Various gene expression patterns of CDKs and CDKLs in different peanut tissues suggested their involvement during growth and development. In addition, qRT-PCR analysis demonstrated that most representing CDK and CDKL gene family members were significantly down-regulated under ABA, PEG and mannitol treatments.ConclusionsGenome-wide analysis offers a comprehensive understanding of the classification, evolution, gene structure, and gene expression profiles of CDK and CDKL genes in cultivated peanut and their diploid progenitors. Additionally, it also provides cell cycle regulatory gene resources for further functional characterization to enhance growth, development and abiotic stress tolerance.

Bacteroid Development, Transcriptome, and Symbiotic Nitrogen-Fixing Comparison of Bradyrhizobium arachidis in Nodules of Peanut (Arachis hypogaea) and Medicinal Legume Sophora flavescens.

Bradyrhizobium arachidis strain CCBAU 051107 could differentiate into swollen and nonswollen bacteroids in determinate root nodules of peanut (Arachis hypogaea) and indeterminate nodules of Sophora flavescens, respectively, with different N2 fixation efficiencies. To reveal the mechanism of bacteroid differentiation and symbiosis efficiency in association with different hosts, morphologies, transcriptomes, and nitrogen fixation efficiencies of the root nodules induced by strain CCBAU 051107 on these two plants were compared. Our results indicated that the nitrogenase activity of peanut nodules was 3 times higher than that of S. flavescens nodules, demonstrating the effects of rhizobium-host interaction on symbiotic effectiveness. With transcriptome comparisons, genes involved in biological nitrogen fixation (BNF) and energy metabolism were upregulated, while those involved in DNA replication, bacterial chemotaxis, and flagellar assembly were significantly downregulated in both types of bacteroids compared with those in free-living cells. However, expression levels of genes involved in BNF, the tricarboxylic acid (TCA) cycle, the pentose phosphate pathway, hydrogenase synthesis, poly-β-hydroxybutyrate (PHB) degradation, and peptidoglycan biosynthesis were significantly greater in the swollen bacteroids of peanut than those in the nonswollen bacteroids of S. flavescens, while contrasting situations were found in expression of genes involved in urea degradation, PHB synthesis, and nitrogen assimilation. Especially higher expression of ureABEF and aspB genes in bacteroids of S. flavescens might imply that the BNF product and nitrogen transport pathway were different from those in peanut. Our study revealed the first differences in bacteroid differentiation and metabolism of these two hosts and will be helpful for us to explore higher-efficiency symbiosis between rhizobia and legumes. IMPORTANCE Rhizobial differentiation into bacteroids in leguminous nodules attracts scientists to investigate its different aspects. The development of bacteroids in the nodule of the important oil crop peanut was first investigated and compared to the status in the nodule of the extremely promiscuous medicinal legume Sophora flavescens by using just a single rhizobial strain of Bradyrhizobium arachidis, CCBAU 051107. This strain differentiates into swollen bacteroids in peanut nodules and nonswollen bacteroids in S. flavescens nodules. The N2-fixing efficiency of the peanut nodules is three times higher than that of S. flavescens. By comparing the transcriptomes of their bacteroids, we found that they have similar gene expression spectra, such as nitrogen fixation and motivity, but different spectra in terms of urease activity and peptidoglycan biosynthesis. Those altered levels of gene expression might be related to their functions and differentiation in respective nodules. Our studies provided novel insight into the rhizobial differentiation and metabolic alteration in different hosts.

Pest Detection and Classification in Peanut Crops Using CNN, MFO, and EViTA Algorithms

Pest Detection and Classification in Peanut Crops Using CNN, MFO, and EViTA Algorithms

Population dynamics, vertical distribution and damage characterization of burrower bug in peanut

Although the burrower bug (Cyrtomenus mirabilis) is considered a vitalsoil pest in peanut crops, Arachis hypogaea L., in South America, there is little information on its occurrence and damage characterization. This study aimed to evaluate the vertical distribution and fluctuation of the burrower bug in the soil and the damage this species causes in peanuts. Two peanut cultivars (IAC OL3 and IAC 503) were evaluated in three locations in the state of São Paulo: Pindorama, Ribeirão Preto and Votuporanga, in the 2017/18 and 2018/19 harvests. Trenches were dug 0.5 m long, 0.3 m wide and 0.3 m deep, along plant lines at four spots on each sampling date, and stratified in layers 10 cm deep. Plant samples (0.5 m) were collected and evaluated for number of pods and percentage of kernels with symptoms of insect damage. More than 85 % of the C. mirabilis population was found in depths of up to 10 cm, especially after plant fructification, and an increase in nymphs from 100 days after sowing (DAS) was seen when an increased number of maturing pods was observed. The occurrence of nymphs and adults of C. mirabilis and their damage to peanut kernels was similar in both cultivars (IAC OL3 and IAC 503), when these were harvested according to their developmental cycles.

Functional traits and phylogenetic analysis of top-soil inhabiting rhizobacteria associated with tea rhizospheres in North Bengal, India

Rhizobacteria associated with cultivated crops are known to stimulate plant growth through various indirect or direct mechanisms. In recent years, the host list of plant growth promotion/promoting rhizobacteria has expanded to include bean, barley, cotton, maize, rice, vegetables, peanut, rice, wheat, and several plantation crops. However, interaction of rhizobacteria with tea plants of organic and conventional tea gardens is poorly understood. In the present study, rhizobacterial species associated with tea rhizosphere were isolated from 14 tea gardens located in North Bengal, India. In total, 16 rhizobacterial isolates isolated from collected soil samples were assessed for antagonistic and plant growth promotion/promoting activity under laboratory conditions. Molecular characterization based on sequencing of 16S rRNA gene revealed dominance of Bacillus with five species followed by Pseudomonas with two species. Interestingly, only one isolate was affiliated with actinobacteria, i.e., Microbacterium barkeri. Out of 16 isolates, isolates Bacillus subtilis OKAKP01, B. subtilis BNLG01, B. paramycoides BOK01, M. barkeri BPATH02, and Stenotrophomonas maltophilia BSEY01 showed highest growth inhibition against Fusarium solani (68.2 to 72.8%), Pseudopestalotiopsis theae (71.1 to 85.6%), and Exobasidium vexans (67.4 to 78.3%) causing respective Fusarium dieback, gray blight, and blister blight diseases in tea crop. Further, these five isolates also possessed significantly greater antifungal (siderophore producer, protease, chitinase, and cellulase activity) and plant growth promotion/promoting (indole-3-acetic acid production, ACC deaminase, ammonia, and phosphate solubilization) traits over other eleven rhizobacterial isolates. Therefore, these five isolates of rhizobacteria were chosen for their plant growth promotion/promoting activity on tea plants in nursery conditions. Results from nursery experiments revealed that these five rhizobacteria significantly improved growth rates of tea plants compared with the control. Therefore, this study suggests that these rhizobacteria could be used to formulate biopesticides and biofertilizers, which could be applied to sustainable tea cultivation to improve crop health and reduce disease attack.

Climatological impact on groundnut crop over Junagadh district

The present study investigated the climatic vulnerability of the peanut cropping system in Gujarat's Junagadh district. The Junagadh district is situated in western Gujarat between the latitudes of 70.150 and 70.550 and the longitudes of 20.470 and 21.450. One of the significant oil seed crops in India is the groundnut crop. Gujarat is India's top groundnut producer. Gujarat's Junagadh district produces the majority of the state's ground net crops. Planning for climate change mitigation efforts requires an understanding of the past, present, and projected temperatures and precipitation in the Junagadh district. IMD provided historical daily temperature data with a resolution of 100 km for the years 1951 to 2019.Historical daily temperature data from 1951 to 2019 is downscaled to 1 km from IMD at a resolution of 100 km x 100 km. Data on historical precipitation was downscale from Cru's 25 km x 25 km resolution to 10 km. The temperature projections (RCP 4.5 and RCP 8.5) for five South Asian codex domain models at resolutions of 50 km x 50 km and scaled down to 1 km are displayed. 50 km x 50km and 10km downscale daily data from five South Asian codex domain models. The Taylor diagram demonstrated that, when tested against cru data, all models perform better in terms of rainfall and temperature. The cm5a model outperformed the other models in terms of performance. Maximum temperatures are seen to rise from 32.760 C to 35.50 C (RCP 4.5) and 37.20 C (RCP 8.5) and minimum temperatures are seen to rise from 23.920 C to 25.300 C (RCP 4.5) and 27.480 C between 1970 and 2100 (RCP 8.5). June, a crucial month for the germination stage of groundnut crops, sees an increase in both the maximum and minimum temperatures. From 1970 to 2100, June to November total precipitation increased from 853.2 mm to 1029 (RCP 4.5) and 1097 mm, respectively (RCP 8.5).

Influence of biochar amendments on the soil quality indicators of sandy loam soils under cassava–peanut cropping sequence in the semi-arid tropics of Northern Lombok, Indonesia

Low nutrient retention and soil organic matter depletion are the major challenges of the cropping system in the sandy loam soils of Northern Lombok, Indonesia. A field experiment was conducted to evaluate the influence of biochar-based organic amendments on the soil quality of sandy loam soils under cassava (Manihot Esculenta, Crants)–peanut (Arachis Hypogeae L.) cropping sequence. The treatments were as follows: biochar (10 ton ha<sup>-1</sup>) and rice straw (3 ton ha<sup>-1</sup>) (B1); biochar (10 ton ha<sup>-1</sup>), cattle manure (10 ton ha<sup>-1</sup>), and rice straw (3 ton ha<sup>-1</sup>) (B2); biochar (10 ton ha<sup>-1</sup>) and cattle manure (10 ton ha<sup>-1</sup>) (B3); biochar (10 ton ha<sup>-1</sup>) and cattle manure (10 ton ha<sup>-1</sup>) plus rice straw mulch (3 ton ha<sup>-1</sup>) applied on surface soils (B4), and without organic amendments (B0) as control. Results showed that the biochar-based organic amendments significantly improved several soil quality indicators such as SOC, total N, available P, Ca, cation-exchange capacity (CEC), and aggregate stability but had no significant effect on pH, K, and Mg. Improvement in soil quality was strongly indicated by an increase in the growth and yield of cassava and peanuts. Treatments B1, B2, B3, and B4 generally had a comparable effect on soil parameters and tended to improve the growth and yield of cassava and peanuts. Cassava was responsive to treatments B2 (biochar, cattle manure, and rice straw) and B3 (biochar and cattle manure) with its actual yield of 27 tons ha<sup>−1</sup>, which is a 40% increase compared with that in the control. As a secondary crop growing after cassava, peanuts also exhibited higher yields in all amended plots compared with that in the control. The highest yield was obtained in B2 (1.38 ton ha<sup>−1</sup>), followed by B4 (1.36 ton ha<sup>−1</sup>), B1 (1.33 ton ha<sup>−1</sup>), and B3 (1.25 ton ha<sup>−1</sup>). In conclusion, the incorporation of biochar, cattle manure, and crop residues (rice straw) into soils is a promising option to maintain soil quality and sustainably produce cassava and peanuts in the sandy loam soils of the semi-arid tropics of Lombok, Indonesia.

EFFECT OF PLANTING DISTANCE AND PRODUCTION OF PEANUT (Arachis hypogea L) SUBJECTED WITH ORANGE PLANTS IN KEPPO VILLAGE,

This study aims to find out the influence of planting distance on the growth and yield of peanut crops and optimal planting distances that can increase peanut growth. This research was conducted in Keppo Village of Pamekasan District Galis District November 2021 - January 2022. The research design uses a RandomIzed Group Design (RAK) which consists of 5 types of planting systems with 4 repeats so that there are 20 plots of treatment. Data that has been obtained using diversity analysis (ujiF) with a 5%. If you get a real influence, then continue to use BNT (Small Difference) with a 5% The results showed that the influence of the planting distance of peanuts that are superimposed with citrus plants has a noticeable influence on variable number of pods, weight of land pods, weight of 100 pods and crop yield perhektar. The most optimal peanut planting distance of all treatments is JT 3 (40 x 20 cm).

A Nationwide Study of Residual Fate of Fluxapyroxad and Its Metabolites in Peanut Crops Across China: Assessment of Human Exposure Potential.

Elaborating on the residual fate of fluxapyroxad and its metabolites based on their nationwide application was vital to protect the human population from their hazardous effects. In this study, a rapid and sensitive analytical method was developed to trace fluxapyroxad and two of its metabolites in peanut matrices using an ultrahigh chromatography method coupled with mass spectrometry (UHPLC−MS/MS) within 3.5 min. The occurrence, pharmacokinetic degradation and terminal magnitudes of fluxapyroxad were reflected in the original deposition of 8.41−38.15 mg/kg, half−lives of 2.5−8.6 d and final concentrations of 0.004−37.38 mg/kg in peanut straw. The total concentrations of fluxapyroxad in peanut straw (0.04−39.28 mg/kg) were significantly higher than those in peanut kernels (<0.001−0.005 mg/kg) and an obvious concentration effect was observed in fresh (0.01−11.56 mg/kg) compared dried peanut straw (0.04−38.97 mg/kg). Fluxapyroxad was demethylated to 3−(difluoromethyl)−N−(3′,4′,5′−trifluoro[1,1′−biphenyl]−2−yl)−1H−pyrazole−4−carboxamide (M700F008, 0.02−5.69 mg/kg) and further N−glycosylated to 3−(difluoromethyl)−1−(ß−D−glucopyranosyl)−N−(3′,4′,5′−triflurobipheny−2−yl)−1H−pyrzaole−4−carboxamide (M700F048, 0.04−39.28 mg/kg).The risk quotients of the total fluxapyroxad for the urban groups were significantly higher than those for the rural groups, and were both negatively correlated with the age of the groups, although both acute (ARfD%, 0.006−0.012%) and chronic (ADI%, 0.415−1.289%) risks are acceptable for the human population. The high-potential health risks of fluxapyroxad should be continuously emphasized for susceptible toddlers (1−3 years), especially those residing in urban areas.

Evaluation of the ef fect of native arbuscular mycorrhizal fungi an vermicompost leachate on the yield and quality of f ield-grown peanuts

The improvement of the symbiosis generated by arbuscular mycorrhizal fungi (AMF) against pathogenic microorganisms has been described, however, the ef fect of AMF in peanut crops is of interest due to the value of the productive chain that has not been fully attended. Therefore, this research work evaluated six treatments: (T1) Control plants without AMF, (T2) Plants with vermicompost leachate, (T3) Plants inoculated with commercial mycorrhizae, (T4) Plants inoculated with native mycorrhizae, (T5) Plants inoculated with commercial mycorrhizae + leachate and (T6) Plants inoculated with commercial mycorrhizae + native mycorrhizae; in this sense, mycorrhizal fungi were isolated, identifying Rhizoglomus clarum, Acaulospora alpina and Acaulospora af f. bireticulata, and inoculated in peanut plants in an area of 144 m2 in the open, so that, to corroborate the incidence of the treatments evaluated, agronomic variables such as height, width of biomass, foliar damage by Cercospora personata, number of f lowers, were evaluated during the pre-harvest every 15 days and the post-harvest variables were; leaf area index, root length, fruit and lipid yield, as well as the prof ile of fatty acids present in the fruit. Finally, peanut quality was evaluated according to CODEX STAN 200-1995, showing that the best treatment was leaching + commercial mycorrhizae, reporting yields of 65.05% of fruits and 58% of total lipids, mostly monounsaturated fatty acids (52 % oleic acid) followed by polyunsaturated (21% linoleic acid), f inally according to CODEX STAN 200-199 the peanuts obtained correspond to the basic quality with dimensions of 4 to 4.5 cm wide and long respectively, these peanuts cannot be exported but they can be marketed in the country for agro-industrial transformation, allowing improved agricultural practices by reducing the use of agrochemicals.

Metabolomics combined with transcriptomics analyses of mechanism regulating testa pigmentation in peanut.

Peanut testa (seed coat) contains large amounts of flavonoids that significantly influence seed color, taste, and nutritional qualities. There are various colors of peanut testa, however, their precise flavonoid components and regulatory mechanism of pigmentation remain unclear. In this study, a total of 133 flavonoids were identified and absolutely quantified in the seed coat of four peanut cultivars with different testa color using a widely targeted metabolomic approach. Black peanut skin had more types and substantial higher levels of cyanidin-based anthocyanins, which possibly contribute to its testa coloration. Procyanidins and flavan-3-ols were the major co-pigmented flavonoids in the red, spot and black peanuts, while flavanols were the most abundant constitutes in white cultivar. Although the concentrations as well as composition characteristics varied, the content ratios of procyanidins to flavan-3-ols were similar in all samples except for white peanut. Furthermore, MYB-like transcription factors, anthocyanidin reductases (ANR), and UDP-glycosyltransferases (UGT) were found to be candidate genes involved in testa pigmentation via RNA-seq and weighted gene co-expression network analysis. It is proposed that UGTs and ANR compete for the substrate cyanidin and the prevalence of UGTs activities over ANR one will determine the color pattern of peanut testa. Our results provide a comprehensive report examining the absolute abundance of flavonoid profiles in peanut seed coat, and the finding are expected to be useful for further understanding of regulation mechanisms of seed coat pigmentation in peanut and other crops.

Prospects for the use of natural transgenic cultivated peanut (&lt;i&gt;Arachis hypogaea&lt;/i&gt; L.) in breeding

Common peanut (Arachis hypogaea L.), or peanut, is an annual herbaceous plant from the legume family (Fabaceae). Russia is one of the largest buyers of peanuts, and at the same time, in the south of the country, a number of zones meet the requirements for the cultivation of this crop. However, at present there are no commercial peanut crops in Russia, and selection work is almost not carried out. It is necessary to identify a new source material for breeding and breeding new high-yielding varieties. In the genomes of species of the genus Arachis, homologues of agrobacterial opine synthase genes, cucumopine synthase (cus) and deoxyfructosylglutamine synthase (mas2`), have been identified. The expression of these genes can affect the economically valuable traits of the plant, since the synthesis of various opines affects the composition of the microbiome in the rhizosphere. We have analyzed the expression of the cus gene in various organs of 9 peanut lines from the VIR collection, which have different geographical origin, belong to different cultivar types, and differ in morphological characters. As a result of the analysis, the organ-specific expression was shown; samples were identified that were contrasting in terms of the level of cus gene expression, including those with a high level of expression in the roots (kk-168, 416, 751). For the first time, data were obtained on the work of the cus gene at different stages of plant development on accessions k-168 and k-1157. An increase in the level of expression in the roots during flowering was revealed; during seed germination, the expression is lower. Further analysis and search for a correlation between the expression level of the cus gene and the manifestation of economically valuable traits in peanuts can provide new material for creating promising varieties.&#x0D; The studies were carried out using the equipment of the resource center of the Science Park of Saint Petersburg State University Development of molecular and cellular technologies.&#x0D; This work was supported by the Russian Science Foundation grant No. 21-14-00050.

Incidence and post-embryonic development of Raoiella macfarlanei Pritchard and Baker (Acari: Tenuipalpidae) on Syzygium Jambos

The genus Raoiella represents one of the important genera of the family Tenuipalpidae, which comprises half a dozen species. Members of this group are known to infest a variety of economically important plants such as coconut, aereca nut, Eucalyptus, olive, peanut and various fruit crops. Among these, the species Raoiella macfarlanei Pritchard and Baker has been recorded on olive, Syzygium jambos and widely distributed in India and Africa.The report of the present study revealed that R. macfarlanei has been recognized as a minor pest and widely distributed in various localities of Kerala, India. A perusal of available literature shows a lack of information on its incidence and biology as it has been given the status of an economic species. But in the present study a high infestation was noticed in the field by the development of brownish patches on the infected leaves, causing severe damage to the host plant. Older leaves were more susceptible to mite attack than the younger ones. Maximum population of R. macfarlanei was found attained during the months of February, March and April. The study also revealed that R. macfarlanei completed its development from egg to adult within 16 days on the host plant, S. jambos.&#x0D;