Green nanoparticles represent a sustainable and eco-friendly approach to developing larvicides targeted at controlling mosquito populations. These nanoparticles are synthesized using natural, non-toxic materials and environmentally benign processes, emphasizing their potential to combat mosquito larvae while minimizing adverse effects on the ecosystem. This approach aligns with the growing need for sustainable solutions in vector control, providing an effective alternative to traditional larvicides with reduced environmental impact. The aim of the present investigation we have utilized the leaf from Laureliopsis philippiana (LP) to synthesize silver (Ag) and palladium (Pd) nanoparticles (NPs) and studied toxicity on deadly disease vectors larvae of Aedes aegypti and Anopheles stephensi. Biosynthesized Ag and Pd NPs were extensively characterized through physical and chemical methods. Gas chromatography–mass spectrometry analysis of L. philippiana assisted in identifying the secondary metabolites of the extract's total flavonoid and phenolic compounds, which are in the reactants of the bio-reduction process during the production of NPs. The in-silico analysis was conducted on mosquito sterol carrier protein-2 (1PZ4) and mosquito odorant binding protein (2L2C). The biosynthesized Ag and Pd NPs revealed the UV absorption peak at 417 and 370 nm, and X-ray diffraction results showed the crystalline structure of NPs. At the same time, in transmission electron microscopy representation, the Ag and Pd NPs were spherical, clustered, and exhibited a noticeable average size of Ag NPs: 13.25 ± 2.53 nm and Pd NPs: 4.07 ± 1.64 nm. The biogenic Ag and Pd NPs showed concentration-dependent larvicidal mortality at 12 and 24-hour exposure with LC50 values of 29.596, 17.804, and 23.915, 14.878 µg/mL on Ae. aegypti and 23.946, 13.938, 19.457, and 12.876 µg/mL against An. stephensi, correspondingly. In-silico studies proved that Laureliopsine A and isoterpinolene showed significant docking scores compared to permethrin on mosquito proteins (1PZ4 and 2L2C). Besides, the LP-Ag and Pd NPs non-toxicity analysis was carried out on zebrafish (Danio rerio) embryos at 72 h post-fertilization. Overall, the findings clearly demonstrated the green synthesized Ag and Pd NPs from L. philippiana to develop environmentally secure and safe larvicides.
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