Objective. To explore the application of evidence-based nursing (EBN) intervention combined with PBL teaching mode in acute myocardial infarction (AMI) nursing and the effects on quality of life (QOL) and satisfaction of patients. Methods. Ninety AMI patients enrolled in our hospital (January 2019-January 2021) were chosen as the study subjects and equally randomized into group M and group N. Group N received conventional nursing, and group M received EBN combined with PBL teaching mode. The cardiac function indexes, self-care ability, disease awareness and nursing satisfaction were compared between the two groups. The self-care ability was scored by the Exercise of Self-Care Agency Scale (ESCA). The QOL of both groups before and after intervention was scored by the Generic Quality of Life lnventory-74 (GQOLI-74). The emotional state before and after intervention was evaluated by the Hospital Anxiety and Depression Scale (HAD). Results. After nursing, cardiac function indexes were markedly higher in group M than group N (P<0.001). Compared with group N, group M achieved notably higher ESCA scores (P<0.001), higher awareness of disease knowledge (P<0.001) and higher nursing satisfaction (P<0.05). After intervention, the GQOLI-74 score in group M was obviously higher while the HAD score was lower compared with group N (P<0.001). Conclusion. The implementation of EBN combined with PBL teaching model in AMI patients can effectively improve nursing satisfaction and QOL as well as alleviate the negative emotions, so as to create a harmonious nurse-patient relationship. Therefore, it is worth applying and promoting.