Pb Concentrations In Plants Research Articles

Sepiolite, Palygorskite, and Triple Super Phosphate on the Uptake of Heavy Metals by Two Iranian Wheat Cultivars Inoculated With Piriformospora indica Fungus

Background and Purpose: Soil remediation in contaminated soils is an important factor of environmental research. This research evaluated the effect of sepiolite, palygorskite, and triple super phosphate (TSP) on decreasing the heavy metals uptake by wheat cultivars inoculated with Piriformospora indica (P. indica). Materials and Methods: Treatments included applying sepiolite, palygorskite (at the rate of 0% and 5% (W/W), and TSP (0% and 0.5% W/W) in the Pb- and Zn-polluted soil under cultivation of 2 wheat cultivars (Mihan and Pishgam cultivars) that inoculated with P. indica fungus. Plants were harvested after 90 days, and Pb and Zn concentrations in soils and plants were measured using atomic absorption spectroscopy. Furthermore, the catalase (CAT) enzyme activity was also measured. Results: With applying 5% (W/W) sepiolite and palygorskite, the plant Pb concentration was significantly reduced by 11.8%, while the plant Zn concentration increased by 13.9%. Plant Zn and Pb concentrations considerably increased and decreased following plant inoculation. However, plant cultivars showed different results. Our results showed that the Pb and Zn concentrations were lower in Mihan than in the Pishgam wheat cultivar. In addition, increasing soil Pb availability caused a significant increase in CAT enzyme activity. Soil contamination with heavy metals had a negative impact on plant root colonization. Conclusion: The interaction of plant cultivars and P. indica significantly affected plant heavy metal uptake by plants. However, environmental studies have found that applying organic amendments like nan-clays or TSP can reduce the absorption of Pb by plants.

Effect of Sulfur Granular Municipal Solid Waste, Humic Acid, and Nano Fe-Oxide on Lead Uptake by Plants in a Calcareous Soil in the Presence of Thiobacillus

Soil poisoning with heavy metals is one of the most significant aspects of environmental research. In the soil treated with Thiobacillus, the effect of sulfur granular municipal solid waste (sulfur granular MSW), humic acid, and nano Fe-oxide on reducing Pb absorption by plants was investigated. Treatments consisted of applying sulfur granular MSW (0 and 30 t/ha), spraying humic acid (0 and 1.5 mmol/L), and using nano Fe-oxide (0 and 2% w/w) in the soil contaminated with Pb (0, 600, and 1200 mg/kg-soil). Plants were collected after 5 months, and the Pb content in the soil and plants was estimated by atomic absorption spectroscopy (AAS). The activity of ascorbate peroxidase (APX) and peroxidases (POX) enzymes were also determined. The use of 30 t/ha of sulfur granular MSW in the Pb-polluted soil reduced Pb concentration in the soil and plant by 13.4% and 15.1 %, respectively, while it increased Fe concentration by 15.2% in the plant. Using nano Fe-oxide (2% w/w) in the Pb-contaminated soil (600 and 1200 mg/kg soil) considerably enhanced Fe content in the plant. Pb absorption by plants was dramatically reduced by foliar application of humic acid. According to the findings of this study, the interaction effects of sulfur granular MSW, nano Fe-oxide, and humic acid significantly reduced the Pb absorption by plants. However, the impacts of soil physico-chemical characteristics and the kind of soil pollutant on plant Pb concentration cannot be ignored.

Reducing lead uptake by plants as a way to lead-free food

In this research, an attempt was made to produce safe food from lead-contaminated soil. It was assumed that an increased amount of calcium (Ca) in plants would prevent them from lead (Pb) uptake. A new-generation agricultural product − an activator of Ca transport in plants “InCa” (from Plant Impact) − was used. The study was conducted on several crop species, Cucumis sativus L., Linum usitatissimum L., Medicago sativa L. and Solanum lycopersicum L., cultivated in mineral medium. The leaves were sprayed with InCa activator while the roots received Pb from the substrate in the form of Pb(NO3)2 dissolved in the medium. It was shown that spraying the leaves with InCa reduced Pb concentration in the roots of S. lycopersicum to 73%, in C. sativus to 60%, and in L. usitatissimum to 57%. Finally, it was found that foliar application of InCa reduced the concentration of Pb in plant roots by 53%, and in plant shoots by 57% (on average by about 55%). These observations were confirmed using histochemical and electron microscopy techniques. It was shown that one of the InCa activator components – Ca(NO₃)₂ – is responsible for such effects. This result was verified by using another experimental method – the Allium epidermis test. Visualization of Pb in epidermal cells of Allium cepa. L. using the Leadmium™Green fluorescent probe (confocal microscopy) showed a reduction in the amount of Pb that entered the epidermal cells after the application of the tested solutions. For the first time, it was shown that it is possible to reduce Pb uptake by plants by up to 55%. In the future, this offers the possibility of developing a foliar calcium preparation aimed at lowering the concentration of Pb in plants and thereby reducing the amount of Pb in the food chain.

Vermicompost Amendment in Soil Affects Growth and Physiology of Zea mays Plants and Decreases Pb Accumulation in Tissues

Minimization of the possible harmful effects of soil pollution on agricultural production and food safety are the major challenges in modern agriculture. There is great scientific interest in the detailed understanding of the physiology of lead uptake and toxicity in Zea mays, together with the search for approaches to minimizing Pb accumulation in tissues. The aim of the present study was to explore the possibility of reducing Pb accumulation in Z. mays plants cultivated in Pb-contaminated soil, by means of vermicompost amendment. Z. mays plants were cultivated at three soil vermicompost amendment rates (10, 20, and 30%), with the addition of 1000 mg L−1 of Pb in the form of Pb(NO3)2 or an equivalent amount of nitrogen in the form of NH4NO3. Additional nitrogen had a significant stimulatory effect on plant growth and physiology, but only for control plants, and at a low vermicompost amendment rate. Independently, Pb had an insignificant negative effect on plant growth and biomass partitioning, but significantly negatively affected the mineral nutrition of Z. mays plants. At a 10 and 20% soil vermicompost amendment rate, the Pb concentration in plant leaves and roots decreased by 65%, while plant biomass increased four to five times in comparison to soil-grown control plants, together with accelerated flowering. It was concluded that vermicompost is one of the most promising soil amendments for reducing heavy metal uptake and accumulation in crop plants, while also being an efficient organic fertilizer.

Pb Transfer Preference of Arbuscular Mycorrhizal Fungus Rhizophagus irregularis in Morus alba under Different Light Intensities.

Arbuscular mycorrhizal (AM) fungi can improve the lead (Pb) tolerance of host plants and accumulate intensive Pb in mycorrhizal roots. However, the detailed contribution of AM fungal extraradical hyphae to the plants' Pb uptake remains unknown. In this study, mulberry (Morus alba) colonized by the AM fungus (Rhizophagus irregularis) with light treatments were linked by fungal extraradical hyphae using a three-compartment system (pot test), and their differences in responding to Pb application were compared. Shading inhibited mulberry photosynthesis and the growth of mulberry. In this study, Pb application did not affect the colonization of R. irregularis when symbiosis had already formed as the root was not exposed to Pb during the colonization and formation of the AM fungal hyphae network. The R. irregularis preferred to transfer more Pb to the unshaded mulberry than to the shaded mulberry, a condition capable of providing more C supply for fungal survival than to low-light mulberry. The Pb transferred through the mycorrhizal pathway to mulberry had low mobility and might be compartmented in the root by R. irregularis until exceeding a threshold. The relatively high expressions of MaABCG16 with high Pb concentrations in plants suggest that MaABCG16 might play an important role in Pb translocation.

Trace Elements Uptake in Brassica rapa chinensis Cultivated in Ultrabasic (Oxisol) and Ultisol Soils, North Borneo

The study aims to investigate the soil physico-chemical properties and trace elements uptake of Brassica rapa chinensis plants cultivated in ultrabasic (oxisol) and ultisoil soil of Ranau, North Borneo, Malaysia by inductively coupled plasma-optical emission spectrometry (ICP-OES). This study can contribute to propose a more sustainable soil management practice. All selected trace elements bioavailability in ultrabasic soil was significantly higher than ultisols except for Ca and Cd. There was no significant amount of difference in soil pH (slightly acidic) and electrical conductivity (slightly saline) between these soil types. However, a significant amount of difference between the percentage of soil moisture and organic matter between ultrabasic and ultisol soils was observed. These might contribute to the uptake of elements into the studied plant. The B. rapa chinensis is a hyperaccumulator of Cd and Pb for both type of soils. The B. rapa chinensis is an accumulator of As when grown in ultrabasic soil, while an accumulator of Co when grown in ultisol soil. Although Pb was not detected in ultisol soil as compared to ultrabasic soil, the concentration of Pb in plants cultivated in ultisol soil had exceeded the maximum permissible limit of Malaysia Food Regulation 1985. Soil amendment in frequently use agriculture sites needs to be conducted such as the addition of organic matter to reduce transfers of trace elements from soil to plants that can pose toxicity risk.

Foliar and soil applications of nanosilicon affected the growth, metals accumulation, and volatile oils of Houttuynia cordata grown in contaminated soils

Cadmium (Cd) and lead (Pb) are the most concerning heavy metals that can cause direct and indirect damage to humans, animals and plants. Thus, there is a need to reduce Cd and Pb accumulation in plants grown in Cd and Pb cocontaminated soils and ensure the quality and safety of foods and plant medicines. The objective of this study was to compare and evaluate the effects of foliar and soil applications of nanosilicon (nano-Si) on plant biomass, metal accumulation and volatile oils in Houttuynia cordata grown in Cd and Pb cocontaminated soil. The results showed that foliar and soil applications of nano-Si significantly increased Si concentration and the dry weight of roots, as well as shoots and leaves in Houttuynia cordata, whereas the Cd and Pb concentrations decreased obviously, except the Cd concentration in the roots. High doses of nano-Si were more effective in reducing Cd and Pb concentrations. Compared with soil application, foliar application of nano-Si was not only more effective but also more economical due to 85% less nano-Si application. In addition, the applications of nano-Si affected the volatile oils in H. cordata by increasing the contents of alkanes and aromatic compounds and reducing the ester contents. Overall, nano-Si foliar application was a suitable approach to simultaneously reduce both the Cd and Pb concentrations in plants grown in Cd and Pb cocontaminated soil by alleviating Cd and Pb toxicity in plant and increasing the plant growth and biomass.

Mitigation of lead (Pb) toxicity in rice cultivated with either ground water or wastewater by application of acidified carbon

Toxicity induced by a high concentration of lead (Pb) can significantly decrease plant's growth, gas exchange, and yield attributes. It can also causes cancer in humans. The use of organic amendments, especially biochar, can alleviate Pb toxicity in different crops. The application of biochar can decrease the uptake of Pb by plant roots. However, the high pH of thermo-pyrolyzed biochar makes it an unfit amendment for high pH soils. As Pb is an acute toxin and its uptake in rice is a major issue, the current experiment was conducted to explore the efficacy of chemically produced acidified carbon (AC) to mitigate Pb toxicity in rice. Lead was introduced in concentrations of 0, 15, and 30 mg kg−1 soil in combination with 0, 0.5, and 1% AC, underground water (GW) and wastewater (WW) in rice plants. The addition of 1% AC significantly improved the plant height (52 and 7%), spike length (66 and 50%), 1000 grains weight (144 and 71%) compared to 0% AC under GW and WW irrigation, respectively at 30 mg Pb kg−1 soil (30 Pb) toxicity. Similar improvements in the photosynthetic rate, transpiration rate and stomatal conductance also validated the effectiveness of 1% AC over 0% AC. A significant decrease in electrolyte leakage and plant Pb concentration by application of 0.5 and 1% AC validates the effectiveness of these treatments for mitigating 30 Pb toxicity in rice compared to 0% AC under GW or WW irrigation. In conclusion, 1% AC is an effective amendment in alleviating Pb toxicity in rice irrigated with GW or WW at 30 Pb.

Derivation of human health risk-based thresholds for lead in soils promote the production of safer wheat and rice

A reliable and accurate soil threshold helps prevent excessive dietary Pb intake risks to consumers of locally grown wheat and rice crops. Based on a three-year investigation of 206 wheat fields and 358 rice fields throughout China, this study aimed to improve the soil protection guidelines by investigating Pb accumulation in soil-wheat and soil-rice systems and by assessing Pb exposure risks through the soil-grain-human pathway. A site-specific bioconcentration factor (BCF, ratio of Pb concentration in plant to that in soil) was calculated and used to assess grain Pb intake risks instead of a generic BCF value to reduce data uncertainty. In addition to soil pH, cation-exchange capacity exerted a major influence on the Pb BCF variations in wheat, whereas the organic carbon dynamics affected the BCF variations in rice. Once normalized BCF against those soil variables, the distributions of BCF were log-normal in nature. Optimizing the pH and cation-exchange capacity of wheat soils would help protect 49.8% of local adults from excessive Pb dietary intake. The scenario soil thresholds linked to soil variables and grain Pb intake risks were then derived and validated by independent data from field surveys and published articles. Poor production practices in the wheat fields under study included using soils with low fertility.

Immobilization of Pb by organic and inorganic phosphate and calcium sources in an acidic Pb-polluted soil amended with cow manure

Background: Chemical stabilization of heavy metals in acidic soil is one of the important points in environmental pollution. Thus, this research was conducted to investigate the effect of organic and inorganic amendments on lead (Pb) immobilization in the Pb-polluted soil. Methods: Treatments were consisted of applying cow manure (0, 15, and 30 t/ha), and applying cow bone and phosphate rock (5% (W/W)) in the Pb (0, 800, and 1600 mg Pb/kg soil)-polluted soil. The plant used in this experiment was canola. After 70 days, the plants were harvested and soil and plant Pb concentration was measured using atomic absorption spectroscopy (AAS). Results: Applying 15 and 30 t/ha of cow manure in the Pb (1600 mg Pb/kg soil)-polluted soil significantly decreased the soil Pb concentration by 14.3 and 17.2%, respectively. For plant Pb concentration, it was increased by 11.8 and 15.1%, respectively. A significant decrease in plant Pb concentration was measured, when the soil under cultivation of the plant was amended with 5% (W/W) phosphate rock powder. For the plants grown on the soil, which was amended with 5% (W/W), the plant Pb concentration decreased by 17.6%. In addition, applying organic and inorganic amendment significantly decreased the bio-concentration factor (BCF), while the soil microbial respiration increased. Conclusion: The results of this study showed that applying 15 and 30 t/ha cow manure or calcium and phosphorus sources such as cow bone and phosphate rock powder (5% (W/W) can decrease the soil Pb availability and prevent the Pb translocation from soil to plants.

Lead (Pb)-resistant bacteria inhibit Pb accumulation in dill (Anethum graveolens L.) by improving biochemical, physiological, and antioxidant enzyme response of plants.

The accumulation of heavy metal in the soil is a serious concern for sustainable food production due to their toxic effects on plants and other living things. The strategies are required on urgent bases for the management of metal-contaminated soils. Thus, the microbes from the genus Pseudomonas were characterized for different traits and lead (Pb)-resistant ability and their effects were assessed on growth, photosynthesis, antioxidant capacity, and Pb uptake by dill (Anethum graveolens L.). Furthermore, soil basal respiration and induced respiration in soil were also assessed under microbes and Pb stress. Among the tested three strains, Pseudomonas P159 and P150 were more tolerant to Pb stress than Pseudomonas P10, whereas P159 showed the highest values for phosphorus (P), siderophore, auxin, and hydrogen cyanide production. The bacterial inoculation increased the plant shoot dry weights, carbohydrates, proline, and chlorophyll contents under Pb stress. The catalase (CAT) and peroxidase (POD) activities of the plants were higher in bacterial-treated plants than control. The bacterial inoculation decreased Pb concentration in plants, and the response varied with the type of microbes. The bacterial strains enhanced the soil basal and induced respiration than respective Pb treatments alone. Overall, Pseudomonas P159 is potentially suitable for the remediation of Pb-contaminated soils. Graphical abstract.

Promotion of growth and phytoextraction of cadmium and lead in Solanum nigrum L. mediated by plant-growth-promoting rhizobacteria

Plant growth-promoting rhizobacteria (PGPR) are a specific category of microbes that improve plant growth and promote greater tolerance to metal stress through their interactions with plant roots. We evaluated the effects of phytoremediation combining the cadmium accumulator Solanum nigrum L. and two Cd- and Pb-resistant bacteria isolates. To understand the interaction between PGPR and their host plant, we conducted greenhouse experiments with inoculation treatments at Nanjing Agricultural University (Jiangsu Province, China), in June 2018. Two Cd- and Pb-resistant PGPR with various growth-promoting properties were isolated from heavy metal-contaminated soil. 16S rRNA analyses indicated that the two isolates were Bacillus genus, and they were named QX8 and QX13. Pot experiments demonstrated that inoculation may improve the rhizosphere soil environment and promote absorption of Fe and P by plants. Inoculation with QX8 and QX13 also enhanced the dry weight of shoots (1.36- and 1.7-fold, respectively) and roots (1.42- and 1.96-fold) of plants growing in Cd- and Pb-contaminated soil, and significantly increased total Cd (1.28–1.81 fold) and Pb (1.08–1.55 fold) content in aerial organs, compared to non-inoculated controls. We also detected increases of 23% and 22% in the acid phosphatase activity of rhizosphere soils inoculated with QX8 and QX13, respectively. However, we did not detect significant differences between inoculated and non-inoculated treatments in Cd and Pb concentrations in plants and available Cd and Pb content in rhizosphere soils. We demonstrated that PGPR-assisted phytoremediation is a promising technique for remediating heavy metal-contaminated soils, with the potential to enhance phytoremediation efficiency and improve soil quality.

Influence of biochar and organic soil amendments on bioavailability and immobilization of copper and lead to common cocklebur in acidic sandy loam soil

Application of amendments may help to alter soil physicochemical properties and the bioavailability of metals. A pot experiment was carried out using a metal accumulator plant common cocklebur (Xanthium strumarium L.). The plant was grown in contaminated soil with copper (Cu) and lead (Pb) at four different concentrations of each metal (150, 250, 300, and 350 mg kg−1 soil). Soil amendments hardwood biochar (HWBC), farmyard manure (FYM), and vermicompost (VC) were applied at 2.77 g kg−1 rate. The amendments were effective in maintaining plant health under metal exposure. VC improved the chlorophyll stability index and dry biomass of plants, which was followed by FYM and HWBC. Decreased in peroxidase activity and metal concentration in plant tissues under the amendments disclosed their effectiveness to reduce metal toxicity. VC application reduced Pb concentration in plant up to 52.94%, while FYM reduced Cu uptake up to 30.03 % by the plant. The speciation study revealed the potentiality of the tested amendments to cause a reduction in bioavailable form of the metals. The order of efficiency to reduce exchangeable fraction of Cu was HWBC (29.2–69.0 %) > FYM (5.9–44.5 %) > VC (5.7–33.8 %). However, to reduce exchangeable fraction of Pb the order was VC (41.8–74.9 %) > FYM (30.2–60.0 %) > HWBC (18.5–26.9 %). Less bioavailable fractions (excluding exchangeable and carbonate bound) of Cu and Pb was found maximum at FYM and VC amendment respectively indicate higher efficacy of them to immobilize Cu and Pb in acidic sandy loam soil inducing reduce possibility of metal contamination in the food chain.

Pertumbuhan, Hasil dan Translokasi Timbal (Pb) pada Tanaman Kangkung (Ipomea reptans) dan Bayam (Amaranthus tricolor L.) Dengan Penambahan Pupuk ZA Berbeda Dosis

Lead (Pb) is the heavy metal that causes the most pollution in the environment. This study was conducted to compare the ability of water spinach and spinach plants to absorb lead due to the administration of various doses of ZA as a heavy metal suppressor on polluted land. This research is a pot experiment using a factorial randomized block design (RBD) with two factors. The first factor is the type of plant (Kangkung and Spinach). The second factor was the dose of ZA fertilizer (0; 1.19; 2.38 3.57; 4.76 g / pot). The variables observed included growth, plant yield, and Pb concentration in plants. The results showed that the growth of kale plants increased with the increasing dose of ZA application up to 2.38 g / pot. Increasing the dose gave an insignificant increase in growth (P <0.05), likewise in spinach plants. However, higher doses caused a significant reduction in the number of spinach leaves (P <0.05). The fresh weight of kale roots and shoots significantly increased by an average of 50.74% and 36.57% compared to the control. While the spinach plant experienced an increase in the average root and shoot fresh weight significantly by 50.76% and 53.10% compared to the control. Water spinach plants accumulated more Pb in the crown with an average Translocation Factor (TF) value of 1.09. Meanwhile, spinach plants accumulated more Pb in their roots with an average TF value of 0.75. These results suggest that the application of ZA increases the Pb uptake of plant hyperaccumulators from vegetables. However, spinach is safer to eat because heavy metals are accumulated in the roots. Keywords: Phyitoremediation, Lead, water spinach, spinach, ZA, Translocation.

Foliar application of silicon nanoparticles affected the growth, vitamin C, flavonoid, and antioxidant enzyme activities of coriander (Coriandrum sativum L.) plants grown in lead (Pb)-spiked soil.

Lead (Pb) is among the most abundant toxic trace elements which causes direct and indirect negative effects on humans, animals, and plants. Thus, there is a need to alleviate the Pb toxicity in plants for good quality food production especially from marginal soils. In this study, the effects of silicon nanoparticles (Si NPs) were investigated on coriander (Coriandrum sativum L.) biomass, vitamin C, flavonoid, antioxidant enzyme activities (i.e., catalase (CAT), peroxidase (POD), and super oxide dismutase (SOD)), malondialdehyde (MDA), and Pb concentration in plants subjected to different Pb concentrations. Treatments included four levels of Pb (0, 500, 1000, and 1500 mg/kg of soil), and two levels of Si NPs (0 and 1.5 mM) in all combinations. The Pb treatments alone decreased the plant biomass and vitamin C while increased the flavonoid, MDA, antioxidant enzyme activities, and Pb concentration in tissues depending upon the Pb treatments. The foliar-applied 1.5 mM Si NPs alleviated the adverse impacts of Pb on coriander plants which were due to the minimization of Pb concentration in plants and improvements in the plant defense system. Si NPs minimized accumulation of MDA in plant tissues and adjusted the activities of POD, CAT, and SOD in plants under Pb stress. Overall, Si NP foliar application might be a suitable approach in reducing the Pb concentrations in plants. However, field studies with various plant species and environmental conditions are required to highlight the role of Si NPs on the plant under toxic trace element stress.

Effects of salicylic acid, humic acid, and EDTA chelate on the increasing Pb concentration in the barley inoculated with PGPR

The present study aimed to investigate the effects of salicylic acid (SA), humic acid (HA), and EDTA chelate on the increasing Pb concentration in a plant inoculated with plant growth-promoting rhizobacteria (PGPR). Treatments consisted of applying two levels of EDTA (0 (EDTA0) and 3 (EDTA3) mmol/kg soil) and soil application of HA (0 (HA0) and 200 (HA200) mg/kg soil). In addition, foliar application of SA at the rates of 0 (SA0) and 1.5 (SA1.5) mmol/lit was also sprayed on the inoculated plant with and without PGPR cultivated in the Pb-polluted soil. After 9 weeks, barley plants were harvested and plant Pb concentration was measured using atomic absorption spectroscopy (AAS). The soil Pb concentration and plant Pb biomass was also measured. The least significant difference (LSD) test was used to determine the differences between the means (P=0.05). The results indicated that application of HA or EDTA had significantly (P=0.05) increased the Pb phytoremediation efficiency, as, applying 3 mmol EDTA/kg soil increased the Pb phytoremediation efficiency by 14.1%. In addition, a significant increasing (P=0.05) in plant biomass and Pb phytoremediation efficiency was observed by 12.2 and 13.6%, respectively, in the inoculated plant cultivated in the soil that received the greatest rates of EDTA and HA together with the highest rate of SA foliar application. Plant growth regulators such as SA or humic acid can increase plant resistance to Pb toxicity and help to increase Pb phytoremediation efficiency that is important in environmental studies.

Decreasing Plant Abiotic Stress in the Soil Treated with Sewage Sludge Using Piriformospora indica Fungus and Multi Walled Carbon Nanotubes

Today, finding suitable methods to decrease plant abiotic stress such as heavy metals or salinity is very necessary in arid and semi-arid regions. Thus, this research was conducted as a factorial experiment in the layout of a completely randomized block design to evaluate the role of Piriformospora indica fungus and multi-walled carbon nanotubes (MWCNTs) on decreasing plant abiotic stress in the soil treated with lead (Pb)-polluted sewage sludge. The treatment consisted of applying sewage sludge at the rates of 0, 15, and 30 t/ha, 3 levels of cadmium spiked in to the applied sewage sludge (0, 800 and 1600 mg Pb/kg), as well as 0%, 0.5%, and 1.5% (W/W) MWCNTs in the presence and absence of P. indica. The plant used in this experiment was barely. After 90 days of the experiment, the plant was harvested and its Pb concentration was measured using atomic absorption spectroscopy. Applying 0.5 and 1% (W/W) MWCNTs in the soil treated with 15 t/ha sewage sludge significantly decreased plant Pb concentration by 8.1% and 12.3%, respectively. In addition, the presence of P. indica had significant effects on decreasing plant Pb concentration since the lowest plant Pb concentration was observed in soil amended with 30 t/ha sewage sludge (800 mg Pb/kg) by receiving 1.5% (W/W) MWCNTs in the presence of P. indica. The results of this study showed that applying MWCNTs has significant effects on decreasing soil heavy metals or soil salinity that is a positive point in environmental studies.

Effects of biochar on availability and plant uptake of heavy metals – A meta-analysis

Biochar can be an effective amendment for immobilizing heavy metals in contaminated soils but has variable effects depending on its chemical and physical properties and those of the treated soil. To investigate the range of biochar's effects on heavy metal accumulation in plants in responses to the variation of soil, biochar and plant, we carried out a meta-analysis of the literature that was published before March 2016. A total of 1298 independent observations were collected from 74 published papers. Results showed that across all studies, biochar addition to soils resulted in average decreases of 38, 39, 25 and 17%, respectively, in the accumulation of Cd, Pb, Cu and Zn in plant tissues. The effect of biochar on heavy metal concentrations in plants varied depending on soil properties, biochar type, plant species, and metal contaminants. The largest decreases in plant heavy metal concentrations occurred in coarse-textured soils amended with biochar. Biochar had a relatively small effect on plant tissue Pb concentrations, but a large effect on plant Cu concentrations when applied to alkaline soils. Plant uptake of Pb, Cu and Zn was less in soils with higher organic carbon contents. Manure-derived biochar was the most effective for reducing Cd and Pb concentrations in plants as compared to biochars derived from other feedstock. Biochar having a high pH and used at high application rates resulted in greater decreases in plant heavy metal uptake. The meta-analysis provides useful guidelines on the range of effects that can be anticipated for different biochar materials in different plant-soil systems.

Lead Concentrations in Soils and Some Wild Plant Species Along Two Busy Roads in Pakistan.

This study assessed the level of Pb in soil and five wild plant species (Calotropis procera, Datura alba, Parthenium hysterophorus, Cenchrus ciliaris and Ricinus communis) during all the four seasons. Two busy roads varying in age and traffic volume were selected i.e., Faisalabad-Sargodha road (FSR) and Pindi Bhattian to Lillah (M-2) in the Punjab, Pakistan. Results showed raised levels of Pb in both plants and soil samples along both roads. The range of Pb concentration in plants was 0.08-3.98 and 1.95-4.74mg kg- 1 for soil. Higher Pb contamination was recorded along FSR road as compared to M-2. Among seasons, the higher Pb concentration was found during summer, probably due to very high temperature. Among all the plants studied, Calotropis procera accumulated the highest level (3.98mg kg- 1 dry wt.) of Pb; Thus, it can be used as good biomonitor/phytoremediator at Pb contaminated areas.

Phytoremediation of As, Ag, and Pb in contaminated soils using terrestrial plants grown on Gumuskoy mining area (Kutahya Turkey)

Gumuskoy Ag (As, Zn and Pb) deposits are located about 25km west of Kutahya, Turkey and one of the largest silver deposits in the country. This study investigated the accumulation and transport of As, Ag, and Pb from the soil into 11 terrestrial plants. Plant and soil samples were collected from the field and As, Ag, and Pb content was measured with inductively coupled plasma mass spectroscopy. The mean values in the soil, roots, and shoots of the studied plants were, respectively, 4771, 2320, and 1340mgkg−1 for As; 35.93, 10.19, and 11.51mgkg−1 for Ag; and 4180, 1424, and 1050mgkg−1 for Pb. The plants collected from the study area were separated into different groups based on the As, Ag and Pb concentrations in plants and their soils. The results showed that Glaucium root, Phlomis root and shoot, Verbascum shoot for As, Glaucium root, Silene root and shoot, Verbascum shoot for Ag and Phlomis root and shoot for Pb could be good a bioaccumulator because of their higher ECRs and ECSs. Therefore, these plants can be useful for cleaning or rehabilitating the soils polluted by Ag, As and Pb.