Root Patterning Research Articles

Novel Insights Into the Hyperaccumulation Syndrome in Pycnandra (Sapotaceae).

The discovery of nickel hyperaccumulation, in Pycnandra acuminata, was the start of a global quest in this fascinating phenomenon. Despite recent advances in the physiology and molecular genetics of hyperaccumulation, the mechanisms and tolerance of Ni accumulation in the most extreme example reported to date, P. acuminata, remains enigmatic. We conducted a hydroponic experiment to establish Ni tolerance levels and translocation patterns in roots and shoots of P. acuminata, and analyzed elemental partitioning to gain insights into Ni regulation. We combined a phylogeny and foliar Ni concentrations to assess the incidence of hyperaccumulation within the genus Pycnandra. Hydroponic dosing experiments revealed that P. acuminata can resist extreme Ni concentrations in solution (up to 3,000 µM), and dosing at 100 µM Ni was beneficial to growth. All plant parts were highly enriched in Ni, but the latex had extreme Ni concentrations (124,000 µg g−1). Hyperaccumulation evolved independently in only two subgenera and five species of the genus Pycnandra. The extremely high level of Ni tolerance is posited to derive from the unique properties of laticifers. The evolutionary and ecological significance of Ni hyperaccumulation in Pycnandra is discussed in light of these findings. We suggest that Ni-rich laticifers might be more widespread in the plant kingdom and that more investigation is warranted.

Fructans Are Differentially Distributed in Root Tissues of Asparagus.

Inulin- and neoseries-type fructans [fructooligosaccharides (FOS) and fructopolysaccharides] accumulate in storage roots of asparagus (Asparagus officinalis L.), which continue to grow throughout the lifespan of this perennial plant. However, little is known about the storage of fructans at the spatial level in planta, and the degree of control by the plant is largely uncertain. We have utilized mass spectrometry imaging (MSI) to resolve FOS distribution patterns in asparagus roots (inner, middle, and outer tissues). Fructan and proteome profiling were further applied to validate the differential abundance of various fructan structures and to correlate observed tissue-specific metabolite patterns with the abundance of related fructan biosynthesis enzymes. Our data revealed an increased abundance of FOS with higher degree of polymerization (DP > 5) and of fructopolysaccharides (DP11 to DP17) towards the inner root tissues. Three isoforms of fructan:fructan 6G-fructosyltransferase (6G-FFT), forming 6G-kestose with a β (2–6) linkage using sucrose as receptor and 1-kestose as donor, were similarly detected in all three root tissues. In contrast, one ß-fructofuranosidase, which likely exhibits fructan:fructan 1-fructosyltransferase (1-FFT) activity, showed very high abundance in the inner tissues and lower levels in the outer tissues. We concluded a tight induction of the biosynthesis of fructans with DP > 5, following a gradient from the outer root cortex to the inner vascular tissues, which also correlates with high levels of sucrose metabolism in inner tissues, observed in our study.

The Plasticity of Root Systems in Response to External Phosphate.

Phosphate is an essential macro-element for plant growth accumulated in the topsoil. The improvement of phosphate uptake efficiency via manually manipulating root system architecture is of vital agronomic importance. This review discusses the molecular mechanisms of root patterning in response to external phosphate availability, which could be applied on the alleviation of phosphate-starvation stress. During the long time evolution, plants have formed sophisticated mechanisms to adapt to environmental phosphate conditions. In terms of root systems, plants would adjust their root system architecture via the regulation of the length of primary root, the length/density of lateral root and root hair and crown root growth angle to cope with different phosphate conditions. Finally, plants develop shallow or deep root system in low or high phosphate conditions, respectively. The plasticity of root system architecture responds to the local phosphate concentrations and this response was regulated by actin filaments, post-translational modification and phytohormones such as auxin, ethylene and cytokinin. This review summarizes the recent progress of adaptive response to external phosphate with focus on integrated physiological, cellular and molecular signaling transduction in rice and Arabidopsis.

Genome-wide identification and expression analysis of extensin genes in tomato

Extensins (EXTs) are major protein components in plant cell walls that play crucial roles in higher plants. The function of EXTs has been reported in several plants but is limited in tomato, especially in fruit ripening. In this study, we identified 83 EXTs in tomato, and divided them into seven groups. The gene intron-exon structure and protein-motif composition of SlEXTs were similar within each group but different among groups. SlEXT genes showed different expression patterns in roots, leaves, flowers and fruits, and some SlEXT gene expressions in flowers could be regulated by treatments of auxin, gibberellic acid and jasmonic acid. In particular, SlSEXT8 had higher and increased expression during tomato fruit ripening, and its expression could be induced by ethylene, suggesting SlSEXT8 may be involved in tomato fruit softening. The result provides insights into the function of EXTs, and will facilitate to further study EXT roles in tomato fruit ripening.

Deep Reinforcement Learning Designed Shinnar-Le Roux RF Pulse Using Root-Flipping: DeepRFSLR.

A novel approach of applying deep reinforcement learning to an RF pulse design is introduced. This method, which is referred to as DeepRFSLR, is designed to minimize the peak amplitude or, equivalently, minimize the pulse duration of a multiband refocusing pulse generated by the Shinar Le-Roux (SLR) algorithm. In the method, the root pattern of SLR polynomial, which determines the RF pulse shape, is optimized by iterative applications of deep reinforcement learning and greedy tree search. When tested for the designs of the multiband pulses with three and seven slices, DeepRFSLR demonstrated improved performance compared to conventional methods, generating shorter duration RF pulses in shorter computational time. In the experiments, the RF pulse from DeepRFSLR produced a slice profile similar to the minimum-phase SLR RF pulse and the profiles matched to that of the computer simulation. Our approach suggests a new way of designing an RF by applying a machine learning algorithm, demonstrating a "machine-designed" MRI sequence.

The plant beneficial rhizobacterium Achromobacter sp. 5B1 influences root development through auxin signaling and redistribution.

Roots provide physical and nutritional support to plant organs that are above ground and play critical roles for adaptation via intricate movements and growth patterns. Through screening the effects of bacterial isolates from roots of halophyte Mesquite (Prosopis sp.) on Arabidopsis thaliana, we identified Achromobacter sp. 5B1 as a probiotic bacterium that influences plant functional traits. Detailed genetic and architectural analyses in Arabidopsis grown in vitro and in soil, cell division measurements, auxin transport and response gene expression and brefeldin A treatments demonstrated that root colonization with Achromobacter sp. 5B1 changes the growth and branching patterns of roots, which were related to auxin perception and redistribution. Expression analysis of auxin transport and signaling revealed a redistribution of auxin within the primary root tip of wild-type seedlings by Achromobacter sp. 5B1 that is disrupted by brefeldin A and correlates with repression of auxin transporters PIN1 and PIN7 in root provasculature, and PIN2 in the epidermis and cortex of the root tip, whereas expression of PIN3 was enhanced in the columella. In seedlings harboring AUX1, EIR1, AXR1, ARF7ARF19, TIR1AFB2AFB3 single, double or triple loss-of-function mutations, or in a dominant (gain-of-function) mutant of SLR1, the bacterium caused primary roots to form supercoils that are devoid of lateral roots. The changes in growth and root architecture elicited by the bacterium helped Arabidopsis seedlings to resist salt stress better. Thus, Achromobacter sp. 5B1 fine tunes both root movements and the auxin response, which may be important for plant growth and environmental adaptation.

Surgically concerned anatomy of roots of lower third molar

Background and Objectives: Root anatomy of lower third molar (LTM) may have variable patterns. Root number, shape and curvature all affecting the process of surgery. To determine frequency of different root patterns of LTM. Patients and methods: Retrospectively, a total of 196 orthopantomograms of department of radiology were evaluated for LTM root number and curvature of roots. Results: two roots 173 (88.26%) one root 22 (11.74%). All single rooted teeth showed no curvature. Among two rooted teeth the study figured out that majority of roots not fused but converge 134 (77.45%), fused together 20 (11.5%), not fused but diverge 11 (≈ 6%), not fused with root curvature. Conclusion: Majority of LTM have two or more roots, they are convergent or divergent. Root anatomy evaluation before surgery is mandatory.

Differential induction of surface chemical compositional change on tooth structure by glass ionomer restorative materials.

Glass ionomer cement (GIC) is a restorative dental material capable of promoting mineral deposition on surrounding tooth substrates. However, it is unclear as to whether demineralization and remineralization due to an oral pH change of the tooth affect the dissolution pattern of tooth crown and root differently. It also remains to be elucidated how GIC alters superficial chemical compositions of tooth crown structurally known as enamel, in relation to the root. In this study, we investigated an effect of pH challenge on chemical compositional change of tooth crown and root, as well as the contribution of GIC restorations on a shift of elemental abundance on tooth crown and root. Our findings demonstrated that an exposure to a pH cycling resulted in a drastic change of elemental profile of the root, but not the crown. Modification of superficial elemental ingredients of GIC-restored cavities located on different anatomical part of the tooth was found after an acid attack. Notably, a differential induction of chemical compositional shift was dependent on the type of GIC used and the location of restored GIC. Our study highlights a susceptibility of root portion to acid-induced elemental dissolution, and that GIC use might be implicated in the delayed dissolution rate of the tooth structure.

Root and Pattern Effects in the Processing of Spoken Non-Words in Arabic

Words in Arabic are formed by mapping roots into patterns. Conducting a series of priming experiments, Boudelaa and Marslen-Wilson (2015, p. 955) have concluded that “root and word pattern morphemes function as abstract cognitive entities, operating independently of semantic factors and dissociable from possible phonological confounds” In the present study, plausibility of this conclusion is tested by investigating native Arabic speakers’ sensitivity to the presence of roots and patterns when processing spoken non-words in Arabic. 50 native Arabic speakers were given a 7-point word-likeness rating task. In this task, participants were asked to rate the word-likeness of 132 auditorily presented non-words in Arabic. 88 of these non-words were created by using real Arabic roots (e.g. /mlk/) that varied in their type and token frequencies and were mapped into two different types of pseudo patterns. Results have shown that native Arabic speakers are sensitive to the presence of roots in the non-words. Specifically, root type frequency had the strongest effect on subjects’ ratings of the non-words in both types of patterns. Implications of these findings to theories of the Arabic mental lexicon will be discussed.

Generic Sequences and the Generic Tonnetz

This article employs concepts from diatonic set theory, transformation theory, and neo-Riemannian theory in an investigation of sequences in generic (mod-7) pitch space. Sequences may be described using generic transposition operators, mod-7 analogs of the more familiar mod-12 chromatic transposition operators, and may be mapped in a generic Tonnetz analogous to the Tonnetz diagrams of neo-Riemannian theory. A complete classification of generic sequences with two-chord transposition blocks is derived from the paths described by the sequences in the Tonnetz. While it is tempting to regard generic space as “diatonic,” the examples demonstrate that generic structure governs many sequences that are not actually diatonic at all. The examples also show that the sequences found in the literature include many more types than are widely recognized and that generic sequence structure may apply in numerous other contexts besides patterns of chord roots.

A historical and comparative review of 50 years of root data collection in Puerto Rico

AbstractFine roots play an important role in plant nutrition, as well as in carbon, water, and nutrient cycling. Fine roots account for a third of terrestrial net primary production (NPP), and inclusion of their structure and function in global carbon models should improve predictions of ecosystem responses to climate change. However, studies focusing on underground plant components are much less frequent than those on aboveground structure. This is more marked in the tropics, where one‐third of the planet's terrestrial NPP is produced. Some tropical forests have been more represented in the literature than others, as demonstrated in the collective studies in Puerto Rico. This Caribbean island's biodiversity, frequency of natural disturbances, ease of access to forests, and long‐term plots have created an ideal place for the study of tropical ecological processes. This literature review emphasizes 50 years of root research and patterns revealed around Puerto Rico. The data in this review were compiled from scientific publications, conference reports, symposiums, and raw data shared by some researches. Emergent patterns include the shallow distribution of fine roots, the great variation in root biomass among different forest types, little variation in root phosphorus concentrations, the slow recovery of root biomass after Hurricane Hugo, and the fact that most data on roots come from the wet tropical Luquillo Experimental Forest, causing other habitat types to be underrepresented. This review also shows the gaps in knowledge about fine roots in the island's ecosystems, which should be used to promote and guide future studies.Abstract in Spanish is available with online material.

Rooting behaviour and soil properties in different bamboo species of Western Himalayan Foothills, India

Due to extensive root system, connected rhizome bamboos are considered suitable for improving soil properties within a short period, though most of the claims are anecdotal and need to be supported with quantified data. The study evaluates seven bamboo species viz., Bambusa balcooa, Bambusa bambos, Bambusa vulgaris, Bambusa nutans, Dendrocalamus hamiltonii, Dendrocalamus stocksii and Dendrocalamus strictus for their rooting pattern and impact on soil health properties. Coarse and fine root intensity was maximum in B. vulgaris. Coarse root biomass ranged from 0.6 kg m−3 in B. nutans to 2.0 kg m−3 in B. vulgaris and B. bambos. Fine root biomass ranged from 1.1 kg m−3 in B. nutans to 4.5 kg m−3 in D. hamiltonii. Contribution of fine roots in terms of intensity and biomass was much higher than coarse roots. Fine root biomass showed declining trend with increase in soil depth in all the species. During sixth year, the litter fall ranged from 8.1 Mg ha−1 in D. stocksii to 12.4 Mg ha−1 in D. hamiltonii. Among soil physical properties significant improvement were recorded in hydraulic conductivity, water stable aggregates and mean weight diameter. Soil pH, organic carbon and available phosphorus under different species did not reveal any significant changes, while significant reduction was observed in total nitrogen and potassium. Significant positive correlation was observed between WSA and iron content. Soil microbial population and enzyme activities were higher in control plot. Considering root distribution, biomass, soil hydraulic conductivity and water stable aggregates, B. bambos, B. vulgaris and D. hamiltonii are recommended for rehabilitation of degraded lands prone to soil erosion.

Comprehensive Analysis of the TIFY Gene Family and Its Expression Profiles under Phytohormone Treatment and Abiotic Stresses in Roots of Populus trichocarpa

The TIFY gene family is specific to land plants, exerting immense influence on plant growth and development as well as responses to biotic and abiotic stresses. Here, we identify 25 TIFY genes in the poplar (Populus trichocarpa) genome. Phylogenetic tree analysis revealed these PtrTIFY genes were divided into four subfamilies within two groups. Promoter cis-element analysis indicated most PtrTIFY genes possess stress- and phytohormone-related cis-elements. Quantitative real-time reverse transcription polymerase chain reaction (qRT–PCR) analysis showed that PtrTIFY genes displayed different expression patterns in roots under abscisic acid, methyl jasmonate, and salicylic acid treatments, and drought, heat, and cold stresses. The protein interaction network indicated that members of the PtrTIFY family may interact with COI1, MYC2/3, and NINJA. Our results provide important information and new insights into the evolution and functions of TIFY genes in P. trichocarpa.

Comparative analysis of basic helix\u2013loop\u2013helix gene family among Brassica oleracea, Brassica rapa, and Brassica napus

BackgroundThe basic helix–loop–helix (bHLH) is the second largest gene family in the plant, some members play important roles in pistil development and response to drought, waterlogging, cold stress and salt stress. The bHLH gene family has been identified in many species, except for Brassica oleracea and B. napus thus far. This study aims to identify the bHLH family members in B. oleracea, B. rapa and B. napus, and elucidate the expression, duplication, phylogeny and evolution characters of them.ResultA total of 268 bHLH genes in B. oleracea, 440 genes in B. napus, and 251 genes in B. rapa, including 21 new bHLH members, have been identified. Subsequently, the analyses of the phylogenetic trees, conserved motifs and gene structures showed that the members in the same subfamily were highly conserved. Most Ka/Ks values of homologous gene were < 1, which indicated that these genes suffered from strong purifying selection for retention. The retention rates of BrabHLH and BolbHLH genes were 51.6 and 55.1%, respectively. The comparative expression patterns between B. rapa and B. napus showed that they had similar expression patterns in the root and contrasting patterns in the stems, leaves, and reproductive tissues. In addition, there were 41 and 30 differential expression bHLH genes under the treatments of ABA and JA, respectively, and the number of down regulation genes was significantly more than up regulation genes.ConclusionIn the present study, we identified and performed the comparative genomics analysis of bHLH gene family among B. oleracea, B. rapa and B. napus, and also investigated their diversity. The expression patterns between B. rapa and B. napus shows that they have the similar expression pattern in the root and opposite patterns in the stems, leaves, and reproduction tissues. Further analysis demonstrated that some bHLH gene members may play crucial roles under the abiotic and biotic stress conditions. This is the first to report on the bHLH gene family analysis in B. oleracea and B. napus, which can offer useful information on the functional analysis of the bHLH gene in plants.

A square root pattern of changes in heart rate variability during the first year after circumferential pulmonary vein isolation for paroxysmal atrial fibrillation and their relation with long‑term arrhythmia recurrence.

An incidental lesion of the parasympathetic ganglia during circumferential pulmonary vein isolation (CPVI) may affect heart rate variability (HRV). We studied the pattern of changes in HRV parameters and the relationship between the 1‑year HRV change following CPVI and the recurrence of atrial fibrillation (AF). A total of 100 consecutive patients undergoing CPVI for paroxysmal AF were enrolled (mean [SD] age, 56 [11.2] years; 61 men). We measured HRV on the day before and after CPVI, and then at 1 month as well as 3, 6, and 12 months after CPVI using 24‑hour Holter monitoring. During the median follow‑up of 33 months, 38 patients experienced the late recurrence of AF (LRAF). Compared with the pre‑CPVI values, HRV was significantly attenuated on day 1 after CPVI in all patients. However, at 3 to 6 months after CPVI, all HRV parameters remained significantly decreased in LRAF‑free patients but not in those with LRAF. The multivariate Cox analysis showed that early AF recurrence within the blanking period (hazard ratio [HR], 4.87; 95% CI, 2.44–9.69; P <0.001) and a change in the standard deviation of normal‑to‑normal intervals (SDNN) observed 3 months after ablation (HR, 0.99; 95% CI, 0.98–1; P = 0.01) were associated with LRAF. The cumulative LRAF freedom after CPVI was greater in patients with an SDNN reduction of more than 25 ms reported 3 months after ablation than in those with a reduction of 25 ms or lower (log‑rank P = 0.004). Sustained parasympathetic denervation during 12 months after CPVI was a marker of successful CPVI, whereas a 3‑month post‑CPVI SDNN reduction of 25 ms or lower predicted LRAF.

Genome-wide identification and characterization of the soybean SOD family during alkaline stress.

BackgroundSuperoxide dismutase (SOD) proteins, as one kind of the antioxidant enzymes, play critical roles in plant response to various environment stresses. Even though its functions in the oxidative stress were very well characterized, the roles of SOD family genes in regulating alkaline stress response are not fully reported.MethodsWe identified the potential family members by using Hidden Markov model and soybean genome database. The neighbor-joining phylogenetic tree and exon-intron structures were generated by using software MEGA 5.0 and GSDS online server, respectively. Furthermore, the conserved motifs were analyzed by MEME online server. The syntenic analysis was conducted using Circos-0.69. Additionally, the expression levels of soybean SOD genes under alkaline stress were identified by qRT-PCR.ResultsIn this study, we identified 13 potential SOD genes in soybean genome. Phylogenetic analysis suggested that SOD genes could be classified into three subfamilies, including MnSODs (GmMSD1–2), FeSODs (GmFSD1–5) and Cu/ZnSODs (GmCSD1–6). We further investigated the gene structure, chromosomal locations and gene-duplication, conserved domains and promoter cis-elements of the soybean SOD genes. We also explored the expression profiles of soybean SOD genes in different tissues and alkaline, salt and cold stresses, based on the transcriptome data. In addition, we detected their expression patterns in roots and leaves by qRT-PCR under alkaline stress, and found that different SOD subfamily genes may play different roles in response to alkaline stress. These results also confirmed the hypothesis that the great evolutionary divergence may contribute to the potential functional diversity in soybean SOD genes. Taken together, we established a foundation for further functional characterization of soybean SOD genes in response to alkaline stress in the future.

Root interactions on sole crop and intercrop give different effects of competition

Root interactions on sole crop and intercrop give different effects of competition. Understanding on root interactions is very challenging due to the methodology limits in root studies especially when we intercrop between tree and crop components. The objectives of this study were (1) to measure the aboveground and belowground biomass of intercropped Acacia hybrid with Beta vulgaris (red beet) and (2) to determine the rooting pattern at each rhizotron depth. This study involved three (3) types of treatments, Acacia hybrid sole crop, Beta vulgaris sole crop, and intercropping of these two species. Root intensity and root biomass were measured. Root growth of red beet was affected by the presence of Acacia hybrid while Acacia hybrid was not affected for both either in sole or intercrop. However, the root intensity of Acacia hybrid was higher in deeper soil layer when intercrop with red beet. For the root biomass, Acacia hybrid was slightly affected when intercrop with red beet. In contract, red beet was higher when intercrop with Acacia hybrid. In terms of shoot biomass, both crops were not affected when they are in sole or intercrop. The rooting pattern showed that the root length density was dominant in the upper layer for both crops, and intercrop treatment was significantly higher compared to sole crops. In conclusion, Acacia hybrid is the competitor to the red beet in belowground interaction in terms of root growth and rooting pattern. There is no differences in terms of aboveground interactions.

Expression of the Legume-Specific Nod Factor Receptor Proteins Alters Developmental and Immune Responses in Rice

Legumes form symbiosis with rhizobia, which fix nitrogen for the benefit of host plant in return for carbon resources. Development of this unique symbiosis in legumes is triggered by rhizobia-secreted nodulation (Nod) factors (NFs). NFs, upon perception, activate Nod signaling cascade, leading to reprogramming of host cell (root) developmental networks to pave way for accommodating rhizobial symbionts. A long-cherished goal of legume-rhizobia symbiosis research is to extend this symbiotic nitrogen-fixing capacity to cereal plants such as rice. As a part of achieving this ultimate goal, in this work, initially we expressed legume-specific Nod factor receptor protein (NFRP) genes, MtNFP, MtLYK3, and LjLNP, in rice and assessed their impact on NF perception and consequently triggered biological responses in roots. RNA-seq analysis revealed that roots of both control and NFRP-expressing plants perceive NFs, but NFs elicited contrasting impacts on gene expression patterns in roots of these plants. In contrast to suppressive role of NFs on expression of several genes involved in innate immune response in roots of control plants, in NFRP-expressing plants, NFs triggered massive upregulation of a vast array of genes associated with signaling, defense response, and secondary metabolism networks in roots. Expression of NFRPs in rice also conferred root hairs the ability to respond to NFs in terms of exhibiting deformations, albeit at low levels. Together, results of the study demonstrated that rice plants have inherent ability to perceive NFs, but the expression of legume NFRPs rendered rice roots hypersensitive to NFs.

A Comparative Evaluation of Efficacy of Electronic Apex Locator, Digital Radiography, and Conventional Radiographic Method for Root Canal Working Length Determination in Primary Teeth: An In Vitro Study.

AimPulpectomy in the primary tooth has unique challenges due to morphological variations in root pattern and physiological root resorption. The electronic apex locator (EAL) is one of the recent methods to determine the estimation of working length without much radiation exposure to the patient as well as the operator. The present study was undertaken for evaluating and comparing the efficacy of EAL, conventional radiography, digital radiography, and actual visual method for the estimation of in the root canal working length (RCL) in extracted primary teeth.Materials and methodsNinety extracted, single-rooted primary teeth were selected. Working length estimation was done with an EAL, conventional, and digital radiographic method, and compared it with an actual visual method.ResultsAccuracy of EAL was observed to be 99.7% followed by digital radiograph (98.1%) and conventional radiograph (96.1%). Both EAL and digital radiographic methods showed a high correlation as compared to conventional. The comparative efficacy of an EAL with a visual method was found to be statistically non-significant (p > 0.005).ConclusionRoot canal working length determined through the electronic method was found to be an accurate and effective tool in single-rooted primary teeth and can be indicated for clinical implementation in endodontic treatment of primary teeth.How to cite this articleSahni A, Kapoor R, Gandhi K, et al. A Comparative Evaluation of Efficacy of Electronic Apex Locator, Digital Radiography, and Conventional Radiographic Method for Root Canal Working Length Determination in Primary Teeth: An In Vitro Study. Int J Clin Pediatr Dent 2020;13(5):523–528.

CBCT Assisted Endodontic Management of Teeth for Aberrant Roots: Case Series

Cone Beam Computed Tomography (CBCT) is a radiographic imaging technique which provides accurate, high-quality, three-Dimensional (3D) images of various parts of head and neck. They deliver images with a spatial resolution of teeth required for diagnosis, treatment planning and postoperative evaluation. The aim of this case series is to describe the importance of determining the root canal morphology with the help of CBCT. This case series presents the endodontic management of a three rooted maxillary first premolar, maxillary first molar with single palatal root and three canals and mandibular first molar with five canals using CBCT. The axial images from CBCT show Gulabivwala K Type I (3-1) canal pattern in distal root of mandibular first molar (36) and palatal root of maxillary first molar (26) according to Al-Quadah Awawdeh showed Type XXII (3-2-1) canal configuration. Biomechanical preparations of all the teeth were done with hand as well as rotary file systems and lateral compaction obturation technique was used. With the advancing technology in imaging, there are possibilities to detect and treat a number of variations particularly in posterior teeth leading to successful endodontic treatment.