Comprehensive Analysis of the TIFY Gene Family and Its Expression Profiles under Phytohormone Treatment and Abiotic Stresses in Roots of Populus trichocarpa

Hanzeng Wang,Xue Leng,Chenghao Li,Xuemei Xu

doi:10.3390/f11030315

Abstract

The TIFY gene family is specific to land plants, exerting immense influence on plant growth and development as well as responses to biotic and abiotic stresses. Here, we identify 25 TIFY genes in the poplar (Populus trichocarpa) genome. Phylogenetic tree analysis revealed these PtrTIFY genes were divided into four subfamilies within two groups. Promoter cis-element analysis indicated most PtrTIFY genes possess stress- and phytohormone-related cis-elements. Quantitative real-time reverse transcription polymerase chain reaction (qRT–PCR) analysis showed that PtrTIFY genes displayed different expression patterns in roots under abscisic acid, methyl jasmonate, and salicylic acid treatments, and drought, heat, and cold stresses. The protein interaction network indicated that members of the PtrTIFY family may interact with COI1, MYC2/3, and NINJA. Our results provide important information and new insights into the evolution and functions of TIFY genes in P. trichocarpa.

Highlights

The TIFY gene family is specific to land plants and was first found in Arabidopsis and annotated as a transcription factor [1,2]
P. trichocarpa genome; in our study, besides the founded 24 PtrTIFY genes, we found another PtrTIFY
WoLF PSORT was used to predict the subcellular location of each candidate TIFY protein in P. trichocarpa

Summary

Introduction

The TIFY gene family is specific to land plants and was first found in Arabidopsis and annotated as a transcription factor [1,2]. This gene family contains a core motif, TIF[F/Y]XG, previously known as ZIM (a zinc-finger protein expressed in inflorescence meristem) [1], and includes 20 members in. Depending on the conservative domain, the proteins are divided into two groups: Group I containing the C2C2-GATA (CX2CX20CX2C). The TIFY family can be divided into four subfamilies: TIFY, JAZ, PPD, and ZML [4]. The TIFY subfamily contains only the TIFY domain. The JAZ subfamily is named for its conserved Jas motif with 22 amino acids, which contains the TIFY domain and a C-terminal conserved domain, as well as the distinctive motif of SLX2FX2KRX2RX5PY [6,7]

Methods

Results

Discussion

Conclusion