One of the important genetic resources of livestock in West Java is Pasundan Cattle. The name of Pasundan Cattle has been determined by Minister of Agriculture of the Republic of Indonesia by Decree No. 1051/Kpts/RI/SR.120/10/2014 as uniformity for local cattle that previously had different names. The productivity of Pasundan Cattle until now has not optimum, especially in the community farmers and research centers. Many factors could be influenced of Pasundan Cattle productivities, including the semen quality of bull as a source and play important role in the breeding program of Pasundan Cattle. Based on this background, it was necessary to conduct this research on the exploration and evaluation of Pasundan Cattle semen quality in three different types of extender. The aim of the research is to find out the best of extender of Pasundan Cattle semen quality. This research was conducted to compare three types of semen extender (TRIS, Citrate and Skim Milk extenders) with addition 20% Egg yolk on each extender. Semen collected two times a week from 9 Pasundan Cattles (3-5 year old), consisted of 9 ejaculated semen and has been evaluated macroscopic and microscopic. Parameter of this research is motility, abnormality, intact plasm membrane and viability of Pasundan Cattle semen. Data analysed with Anova and advance analysis of different between treatment followed with Duncan test. Result of this research showed that there was no significantly differences between TRIS and Citrate (p> .05) on motilty but significant different with Skim milk extender. Otherwise there was significantly different between TRIS and SKIM on intact membrane plasm (p< .05) but not significant different with citrate extender. Meanwhile for abnormality and viability, there were no significantly different on each extenders. As an conclusion, TRIS as the best extender for Pasundan Cattle semen quality.