Part Of Machinery Research Articles

Role of the dynamin-related protein 2 family and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana.

Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth and development through controlling plasma membrane protein composition and cargo uptake. CME relies on the precise recruitment of regulators for vesicle maturation and release. Homologues of components of mammalian vesicle scission are strong candidates to be part of the scission machinery in plants, but the precise roles of these proteins in this process are not fully understood. Here, we characterised the roles of the plant dynamin-related protein 2 (DRP2) family (hereafter DRP2s) and SH3-domain containing protein 2 (SH3P2), the plant homologue to recruiters of dynamins, such as endophilin and amphiphysin, in CME by combining high-resolution imaging of endocytic events in vivo and characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive similarly late during CME and physically interact, genetic analysis of the sh3p123 triple mutant and complementation assays with non-SH3P2-interacting DRP2 variants suggest that SH3P2 does not directly recruit DRP2s to the site of endocytosis. These observations imply that, despite the presence of many well-conserved endocytic components, plants have acquired a distinct mechanism for CME.

The Iranian policy advisory system: Contained politicisation and emerging technicisation

AbstractA lot of literature on policy advisory systems (PASs) is based on Western liberal democracies, with scant attention paid to policymaking systems in authoritarian regimes. This paper examines the Iran's PAS and how it has changed between 1989 and 2023. The paper documents four distinctive phases in Iran's PAS: in‐house ‘institutionalisation’, transitional ‘politicisation’, technocratic ‘externalisation’, and ‘technicisation’. We examine 16 representative advisory organisations (1989–2023) and show that while the Iranian PAS is well institutionalised and increasingly becoming externalised, there is still a tradition of centralisation and limited bureaucratic openness. Further, Iran's PAS has become less political due to constitutional preferences preventing advisory organisations from dealing with politically sensitive issues, as well as the decline of political party traditions and institutions. Finally, the paper concludes with a discussion of the emerging ‘technicisation’ of Iran's PAS, indicating an expansion of and reliance on technology‐assisted tools and instruments by advisory organisations.Points for practitioners Regardless of differences in political systems, policy advisory systems are an indispensable part of public policymaking machinery. To enhance the quality of public policies, policymakers should support a diversified and competent advisory system. To keep externalised organisations alive and effective, they must be supported by public measures and protected by a legal framework. A form of technocratic‐political balance in PAS is necessary. A well‐developed policy advisory system not only includes non‐partisan and technocratic advisory institutions, it also consists of politicised institutions providing political advice for policymakers too.

Multiple metabolic signals in the CeA regulate feeding: The role of AMPK

BackgroundThe central nucleus of the amygdala (CeA) is part of the dopaminergic reward system and controls energy balance. Recently, a cluster of neurons was identified as responsive to the orexigenic effect of ghrelin and fasting. However, the signaling pathway by which ghrelin and fasting induce feeding is unknown. AMP-activated protein kinase (AMPK) is a cellular energy sensor, and its Thr172 phosphorylation (AMPKThr172) in the mediobasal hypothalamus regulates food intake. However, whether the expression and activation of AMPK in CeA could be one of the intracellular signaling activated in response to ghrelin and fasting eliciting food intake is unknown. AimTo evaluate the activation of AMPK into CeA in response to ghrelin, fasting, and 2-deoxy-D-glucose (2DG) and whether feeding accompanied these changes. In addition, to investigate whether the inhibition of AMPK into CeA could decrease food intake. MethodsOn a chow diet, eight-week-old Wistar male rats were stereotaxically implanted with a cannula in the CeA to inject several modulators of AMPKα1/2Thr172 phosphorylation, and we performed physiological and molecular assays. Key findingsFasting increased, and refeeding reduced AMPKThr172 in the CeA. Intra-CeA glucose injection decreased feeding, whereas injection of 2DG, a glucoprivation inductor, in the CeA, increased food intake and blood glucose, despite faint increases in AMPKThr172. Intra-CeA ghrelin injection increased food intake and AMPKThr172. To further confirm the role of AMPK in the CeA, chronic injection of Melanotan II (MTII) in CeA reduced body mass and food intake over seven days together with a slight decrease in AMPKThr172. SignificanceOur findings identified that AMPK might be part of the signaling machinery in the CeA, which responds to nutrients and hormones contributing to feeding control. The results can contribute to understanding the pathophysiological mechanisms of altered feeding behavior/consumption, such as binge eating of caloric-dense, palatable food.

The GTPase activating protein Gyp7 regulates Rab7/Ypt7 activity on late endosomes.

Organelles of the endomembrane system contain Rab GTPases as identity markers. Their localization is determined by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). It remains largely unclear how these regulators are specifically targeted to organelles and how their activity is regulated. Here, we focus on the GAP Gyp7, which acts on the Rab7-like Ypt7 protein in yeast, and surprisingly observe the protein exclusively in puncta proximal to the vacuole. Mistargeting of Gyp7 to the vacuole strongly affects vacuole morphology, suggesting that endosomal localization is needed for function. In agreement, efficient endolysosomal transport requires Gyp7. In vitro assays reveal that Gyp7 requires a distinct lipid environment for membrane binding and activity. Overexpression of Gyp7 concentrates Ypt7 in late endosomes and results in resistance to rapamycin, an inhibitor of the target of rapamycin complex 1 (TORC1), suggesting that these late endosomes are signaling endosomes. We postulate that Gyp7 is part of regulatory machinery involved in late endosome function.

Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms.

Anion transporters sustain a variety of physiological states in cells. Bestrophins (BSTs) belong to a Cl- and/or HCO3- transporter family conserved in bacteria, animals, algae, and plants. Recently, putative BSTs were found in the green alga Chlamydomonas reinhardtii, where they are upregulated under low CO2 (LC) conditions and play an essential role in the CO2-concentrating mechanism (CCM). The putative BST orthologs are also conserved in diatoms, secondary endosymbiotic algae harboring red-type plastids, but their physiological functions are unknown. Here, we characterized the subcellular localization and expression profile of BSTs in the marine diatoms Phaeodactylum tricornutum (PtBST1 to 4) and Thalassiosira pseudonana (TpBST1 and 2). PtBST1, PtBST2, and PtBST4 were localized at the stroma thylakoid membrane outside of the pyrenoid, and PtBST3 was localized in the pyrenoid. Contrarily, TpBST1 and TpBST2 were both localized in the pyrenoid. These BST proteins accumulated in cells grown in LC but not in 1% CO2 (high CO2 [HC]). To assess the physiological functions, we generated knockout mutants for the PtBST1 gene by genome editing. The lack of PtBST1 decreased photosynthetic affinity for dissolved inorganic carbon to the level comparable with the HC-grown wild type. Furthermore, non-photochemical quenching in LC-grown cells was 1.5 to 2.0 times higher in the mutants than in the wild type. These data suggest that HCO3- transport at the stroma thylakoid membranes by PtBST1 is a critical part of the CO2-evolving machinery of the pyrenoid in the fully induced CCM and that PtBST1 may modulate photoprotection under CO2-limited environments in P. tricornutum.

Structure of the human KMN complex and implications for regulation of its assembly

Biorientation of chromosomes during cell division is necessary for precise dispatching of a mother cell’s chromosomes into its two daughters. Kinetochores, large layered structures built on specialized chromosome loci named centromeres, promote biorientation by binding and sensing spindle microtubules. One of the outer layer main components is a ten-subunit assembly comprising Knl1C, Mis12C and Ndc80C (KMN) subcomplexes. The KMN is highly elongated and docks on kinetochores and microtubules through interfaces at its opposite extremes. Here, we combine cryogenic electron microscopy reconstructions and AlphaFold2 predictions to generate a model of the human KMN that reveals all intra-KMN interfaces. We identify and functionally validate two interaction interfaces that link Mis12C to Ndc80C and Knl1C. Through targeted interference experiments, we demonstrate that this mutual organization strongly stabilizes the KMN assembly. Our work thus reports a comprehensive structural and functional analysis of this part of the kinetochore microtubule-binding machinery and elucidates the path of connections from the chromatin-bound components to the force-generating components.

Structure-Based Optimization of Selective and Brain Penetrant CK1δ Inhibitors for the Treatment of Circadian Disruptions.

Neuropsychiatric disorders such as major depressive disorders and schizophrenia are often associated with disruptions to the normal 24 h sleep wake cycle. Casein kinase 1 (CK1δ) is an integral part of the molecular machinery that regulates circadian rhythms. Starting from a cluster of bicyclic pyrazoles identified from a virtual screening effort, we utilized structure-based drug design to identify and reinforce a unique "hinge-flip" binding mode that provides a high degree of selectivity for CK1δ versus the kinome. Pharmacokinetics, brain exposure, and target engagement as measured by ex vivo autoradiography are described for advanced analogs.

Abstract B058: Targeting mediator kinase CDK8/19 potentiates chemotherapeutic responses, reverses tumor growth, and prolongs survival from ovarian clear cell carcinoma

Abstract Ovarian clear cell carcinomas (OCCC) are a rare histological subtype of epithelial ovarian cancer with known treatment resistance, a feature associated with transcriptional reprogramming. CDK8 and CDK19 Mediator Kinases, which are isoforms of the Mediator Kinase – enzymatic components of a regulatory module that binds to the transcriptional Mediator complex –and not a part of the overall transcription machinery, have been previously reported to modify oncogenic transcription factors. Inhibition of the kinase activity of CDK8/19 was shown to prevent and reverse chemoresistance in other cancers with two CDK8/19 inhibitors currently in clinical trials for non–ovarian cancers. Based on the unmet need for OCCCs, we assessed CDK8/19 expression in a panel of OCCC primary and metastatic tissues and utilized cell line models for investigation into the therapeutic potential of CDK8/19 inhibitors in preventing tumorigenesis and synergizing with existing chemotherapeutics. The synergistic effects of SNX631-6, a preclinical CDK8/19 inhibitor drug candidate, were tested in vitro using three clear cell (ES2, TOV-21-G, RMG1) and one non-clear cell (SKOV3) ovarian carcinoma cell lines. A sub-inhibitory concentration 50 (IC50) concentration of SNX631-6 (500 nM) strongly potentiated effects of cisplatin, carboplatin, and paclitaxel. Additionally, cells demonstrating platinum resistance reverted to platinum sensitivity when exposed to the same concentration of SNX631-6. In vivo antitumor effects and tolerability of oral SNX631-6 were assessed in Foxn1nu homozygous female mice injected subcutaneously with ES2+luc cells. Mice were treated with a vehicle (n=10), intraperitoneal (IP) cisplatin (2 mg/kg) twice weekly (n=9), SNX631-6 medicated diet (330 ppm) orally ad libitum (n=10), or IP cisplatin combined with SNX631 diet (n=10) until tumors reached a predetermined critical volume of 2500mm3. In ES2+luc injected mice, significant tumor growth inhibition relative to vehicle occurred in mice treated with a combination of IP cisplatin and SNX631-6 (p<0.001) or SNX631-6 alone (p<0.01). Additionally, the use of IP cisplatin and SNX631-6 significantly delayed time-to-critical-volume compared to vehicle (p=0.0001). SNX631-6 alone and in combination with IP cisplatin was well tolerated in all mice based on body weight measurements. In summary, SNX631-6 potentiated platinum responses in vitro and demonstrated strong in vivo antitumor effects, including tumor regression, when used as monotherapy and in combination with platinum-based therapy. These data suggest that therapeutically achievable doses of SNX631-6 may provide clinical benefit, prevent tumorigenesis, and reverse platinum resistance in patients with OCCC. Citation Format: Wade Barton, Asha Kumari, Gary School, Zachary Mack, Liz Macias Quintero, Karthik Rangavajhula, Rebecca Arend, Mengqian Chen, Eugenia V. Broude, Karthikeyan Mythreye. Targeting mediator kinase CDK8/19 potentiates chemotherapeutic responses, reverses tumor growth, and prolongs survival from ovarian clear cell carcinoma [abstract]. In: Proceedings of the AACR Special Conference on Ovarian Cancer; 2023 Oct 5-7; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(5 Suppl_2):Abstract nr B058.

Molecular insights into codon usage analysis of mitochondrial fission and fusion gene: relevance to neurodegenerative diseases.

Mitochondrial dysfunction is the leading cause of neurodegenerative disorders like Alzheimer's disease and Parkinson's disease. Mitochondria is a highly dynamic organelle continuously undergoing the process of fission and fusion for even distribution of components and maintaining proper shape, number, and bioenergetic functionality. A set of genes governs the process of fission and fusion. OPA1, Mfn1, and Mfn2 govern fusion, while Drp1, Fis1, MIEF1, and MIEF2 genes control fission. Determination of specific molecular patterns of transcripts of these genes revealed the impact of compositional constraints on selecting optimal codons. AGA and CCA codons were over-represented, and CCC, GTC, TTC, GGG, ACG were under-represented in the fusion gene set. In contrast, CTG was over-represented, and GCG, CCG, and TCG were under-represented in the fission gene set. Hydropathicity analysis revealed non-polar protein products of both fission and fusion gene set transcripts. AGA codon repeats are an integral part of translational regulation machinery and present a distinct pattern of over-representation and under-representation in different transcripts within the gene sets, suggestive of selective translational force precisely controlling the occurrence of the codon. Out of six synonymous codons, five synonymous codons encoding for leucine were used differently in both gene sets. Hence, forces regulating the occurrence of AGA and five synonymous leucine-encoding codons suggest translational selection. A correlation of mutational bias with gene expression and codon bias and GRAVY and AROMA signifies the selection pressure in both gene sets, while the correlation of compositional bias with gene expression, codon bias, protein properties, and minimum free energy signifies the presence of compositional constraints. More than 25% of codons of both gene sets showed a significant difference in codon usage. The overall analysis shed light on molecular features of gene sets involved in fission and fusion.

Decolonizing allegory and anti-imperialist critique in the longue durée of extractivism

A re-thinking of the critical vocation of the Journal of Commonwealth Literature is long overdue. The British Commonwealth of Nations that was first established in 1949 has continued to provide a neo-colonial framework for Britain and its former dominions (particularly Australia and Canada) to extract raw materials, capital, and labour from former British colonies and commodity frontiers within settler colonies. For this reason, the British Commonwealth of Nations may be understood as a zombie-like system of extractivism, in which a moribund imperial power stumbles on by draining the postcolonial world of its lifeblood. Against the obfuscation of this system by the term “Commonwealth literature”, I suggest that one of the critical tasks of anti-imperialist critique in future issues of Literature, Critique, and Empire Today is to examine how a dynamic relationship between allegory and counter-allegory in decolonial world literatures works to foreground and contest the neo-colonial dynamics of extractivism, in order to imagine the conditions of possibility for bringing about the abolition of that system. At the core of the article is a consideration of how allegory and counter-allegory form part of the intricate allegorical machinery of two rather different cultural texts: M. NourbeSe Philip’s experimental poem Zong! (2008) and William Kentridge’s animated film Mine (1991). By giving form and meaning to the history and legacy of anti-systemic movements against racial extractivism, decolonizing allegories such as Zong! and Mine also demand a rethinking of predominant materialist approaches to modern allegory.

Tooth backlash inspired comb-shaped single-electrode triboelectric nanogenerator for self-powered condition monitoring of gear transmission

Gear transmissions are an integral part of most rotation machinery. Their abnormalities can affect the reliable operation of the equipment. Most sensors that monitor gear transmissions lack self-powered capability and have weak features due to the restricted mounting locations. In this study, the tooth backlash inspired triboelectric nanogenerator (TB-TENG) is proposed for self-powered condition monitoring of the gear transmissions. A series of comb-shaped copper foil electrodes is arranged on the non-load tooth sides to create a single-electrode TENG. Through the rational use of the tooth backlash space, the TB-TENG does not affect the tooth meshing or participate in the load transmission, ensuring both durability and structural compactness. The TB-TENG outputs are evaluated under various working conditions, structural optimizations, and different working environments based on the established TB-TENG test system. Consequently, the maximum output power density corresponding to the optimal resistance is obtained. Based on the output signal model, the effectiveness of the TB-TENG in self-powered condition monitoring is verified. Moreover, the denoising convolutional auto-encoder (DCAE) is trained based on the TB-TENG voltage signals, achieving a diagnostic accuracy of 98.4%, equivalent to accuracy based on vibration signals. The practical applicability of the proposed TB-TENG is demonstrated through deployment testing in an industrial parallel-gear transmission system. Specifically, TB-TENG can accurately monitor gear speed under variable speed conditions to assess operation stability and health status. Finally, the theoretical and experimental basis for the TB-TENG is provided with applications for self-powered condition monitoring.

Inter-subunit energy transfer processes in a minimal plant photosystem II supercomplex.

Photosystem II (PSII) is an integral part of the photosynthesis machinery, in which several light-harvesting complexes rely on inter-complex excitonic energy transfer (EET) processes to channel energy to the reaction center. In this paper, we report on a direct observation of the inter-complex EET in a minimal PSII supercomplex from plants, containing the trimeric light-harvesting complex II (LHCII), the monomeric light-harvesting complex CP26, and the monomeric PSII core complex. Using two-dimensional (2D) electronic spectroscopy, we measure an inter-complex EET timescale of 50 picoseconds for excitations from the LHCII-CP26 peripheral antenna to the PSII core. The 2D electronic spectra also reveal that the transfer timescale is nearly constant over the pump spectrum of 600 to 700 nanometers. Structure-based calculations reveal the contribution of each antenna complex to the measured inter-complex EET time. These results provide a step in elucidating the full inter-complex energy transfer network of the PSII machinery.

Vibration behavior of a 3D-printed hybrid polymer bearing with outer race defect

PurposeIn industry applications, polymer hybrid bearings have become widespread in recent years owing to the lack of lubricant requirements, particularly in areas requiring hygiene. The additive manufacturing method gives significant advantages to have complex machinery parts, and it has become popular in the industry in recent years. However, it has some inherent disadvantages caused by layered deposition/addition of the materials, and the probability of the localized defect is much higher than in the conventional manufacturing methods. This study aims to investigate the effect of the outer race defect on the characteristics of vibration and service lifetime of hybrid polymer ball bearings produced with the stereolithography (SLA) additive manufacturing method.Design/methodology/approachIn this study, polymer bearings’ races were produced with the additive manufacturing SLA method, and the outer race defect was analyzed with measured vibrations.FindingsThe results show that the additive manufacturing method suggests a practical solution for producing a polymer hybrid ball bearing. On the other hand, the hybrid three-dimensional-printed bearing, which has an outer race defect, worked for approximately 8 h without any problems under a 1 kg load and a shaft speed of around 1,000 rpm. In addition, when there is a defect in the outer and/or inner race of the ball bearing, the crest factor and kurtosis of the vibration are higher than faultless ball bearing, as expected.Originality/valueThis paper provides valuable information on the lifetime and vibration characteristics of polymer hybrid ball bearing produced by means of additive manufacturing.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-06-2023-0183/

Modeling the moving parts of the cellular machinery

Modeling the moving parts of the cellular machinery

The pH domain is an integral part of the catalytic machinery of the GTPase activating ASAP1

The pH domain is an integral part of the catalytic machinery of the GTPase activating ASAP1

Impact of optimization parameters on green sustainable oil from ripe palm kernel seeds for energy utilities: A machine learning approach

In this study, two predicting tools; Artificial Neural Networks, and Machine Learning model were used to optimize and predict the green sustainable oil synthesis from ripe palm kernel seeds utilizing the Soxhlet extractor with n-hexane. The Artificial Neural Networks result indicates that the highest predicted value obtained was 37.769 wt percent at 50 min of extraction time, 175 ml of solvent volume, and 40 g of sample weight, while the highest predicted value from Machine Learning approaches was 38.0354 wt percent at 40 min of extraction time, 200 ml of solvent volume, and 60 g of sample weight. Thus, Machine Learning produced higher yield. Also, the Artificial Neural Networks model's R2 was 0.99922, and the Machine Learning model's R2 was 1 which indicates the muscular nature of Machine Learning. Furthermore, the results of the sensitivity analysis from the machine learning approach based on Python instruction gave an easy decoding of the most crucial input factors and their impact on the predicted values, correlating with other findings from this study. Additionally, the physicochemical assets of green sustainable oil produced from ripe palm kernel seeds falls within acceptable range. Thus, this shows that the Obetim-Uno ripe palm kernel seeds could be used to cool and lubricate whirling machinery parts, in addition to being used to produce green-fuels, when treated with other materials.

Determining the Level of Structural and Mechanical Degradation of Steel in the Supporting Structure of Mining Excavation Machinery

The steels from which the components of mining machinery are constructed must be based primarily on knowledge of their behaviour under environmental conditions and of the excavation technology. Secondly, the structural changes undergone by the materials due to mechanical and thermal processing and the stresses generated by the action of excavation forces that can induce internal stresses at a level that is difficult to determine must be taken into account. Determining the level of structural and mechanical degradation involves sampling the load-bearing structure elements of the excavation and storage machinery for analysis of the mechanical and chemical properties of the component materials. The paper focuses on issues related to the structural and mechanical degradation of steel as a material for parts of mining machinery. In this paper, a methodology for evaluating the degree of structural degradation of three types of bucket wheel excavators with different operating hours (ERc 1400-30/7-07 with 50,400 h, ERc 1400-30/7-08 with 69,264 h, and SRs 1300-26/3.5 with 112,000 h) is proposed. The methodology aims to provide a quantitative assessment of the structural degradation by considering the specific elastic limit of the material used in the machines and the number of hours of operation. This method uses a minimum set of destructive mechanical tests, such as tensile, resilience for three working temperatures, chemical analysis, and durometry on resistance elements taken from the machine, as well as the use of Weibull analysis. By combining this information with the number of hours of operation, a comprehensive evaluation can be made to determine the extent of degradation and potential maintenance requirements. The use of a minimum set of mechanical tests ensures an efficient and cost-effective approach to assessing the structural integrity of these machines. The metallographic analysis highlighted a predominant and defined form of grain rearrangement in the E14-07 machine after only 50,400 h. The constituents identified in the samples taken from the three machines are ferrite and pearlite. The highest hardness of the samples was recorded for the E14-08 machine, with values between 162–165 HV10. For bucket wheel excavators E14-07 and E14-08, the material structure showed elongated grains in the direction of deformation, compared to the material structure of E13-04, which has finely equiaxed grains. As a result of the research, final conclusions were formulated.

Dynamic signal analysis based monitoring for ball screws preload deterioration

The ball screw feed system has been an important part of precision machinery and machine tools. Its preload degradation affects machining accuracy and the issue should be first coped with per manufacturers’ suggestion. The study aims to apply the adaptive order tracking technique and other spectrum analysis schemes as well to differentiate the preload conditions of ball screws. Here varied preload conditions (4 %, 1 % preload and 12um backlash) were considered for monitoring during normal operation. The sideband energy of higher harmonics of ball pass frequency (BPF) are featured to highlight the preload degradation. The control charts together with sideband ratios were established to differentiate preload conditions and single out backlash ball screws.

Genomic basis of melanin-associated phenotypes suggests colour-specific environmental adaptations in tawny owls.

Feathers comprise a series of evolutionary innovations but also harbour colour, a key biological trait known to co-vary with life history or complex traits. Those relationships are particularly true in melanin-based pigmentation species due to known pleiotropic effects of the melanocortin pathway - originating from melanin-associated phenotypes. Here, we explore the molecular basis of melanin colouration and expected co-variation at the molecular level in the melanin-based, colour polymorphic system of the tawny owl (Strix aluco). An extensive body of literature has revealed that grey and brown tawny owl colour morphs differ in a series of life history and behavioural traits. Thus, it is plausible to expect co-variation also at molecular level between colour morphs. To investigate this possibility, we assembled the first draft genome of the species against which we mapped ddRADseq reads from 220 grey and 150 brown morphs - representing 10 years of pedigree data from a population in Southern Finland - and explored genome-wide associations with colour phenotype. Our results revealed putative molecular signatures of cold adaptation strongly associated with the grey phenotype, namely, a non-synonymous substitution in MCHR1, plus 2 substitutions in non-coding regions of FTCD and FAM135A whose genotype combinations obtained a predictive power of up to 100% (predicting grey colour). These suggest a molecular basis of cold environment adaptations predicted to be grey-morph specific. Our results potentially reveal part of the molecular machinery of melanin-associated phenotypes and provide novel insights towards understanding the functional genomics of colour polymorphism in melanin-based pigmented species.

Tungsten coating technologies for agricultural machinery engine parts

The article reviews the possibilities of using tungsten for engine parts of agricultural machinery and the transition to electric motors. The use of electric motors for agricultural machinery and experience in the production of electric tractors is described. A description of the manufacturers of electric tractors and their advantages is given. The materials of motor parts are described, including the use of tungsten. CVD coatings are selected and described as a technology for electric motor applications. The technology of obtaining tungsten coatings on the electrodes of the engine of agricultural machinery by the method of obtaining CVD coatings is proposed. In this article, the CVD coating mode is selected and the properties of the obtained coating according to the selected mode are analysed. The advantages of the coating are identified and the future development of the technology is described.