Parts Catalog Research Articles

Identification of novel broad host-range promoter sequences functional in diverse Pseudomonadota by a promoter-trap approach.

Finding novel promoter sequences is a cornerstone of synthetic biology. To contribute to the expanding catalog of biological parts, we employed a promoter-trap approach to identify novel sequences within an Antarctic microbial community that act as broad host-range promoters functional in diverse Pseudomonadota. Using Pseudomonas putida KT2440 as host, we generated a library comprising approximately 2,000 clones resulting in the identification of thirteen functional promoter sequences, thereby expanding the genetic toolkit available for this chassis. Some of the discovered promoter sequences prove to be broad host-range as they drove gene expression not only in P. putida KT2440 but also in Escherichia coli DH5α, Cupriavidus taiwanensis R1T, Paraburkholderia phymatum STM 815T, Ensifer meliloti 1021, and an indigenous Antarctic bacterium, Pseudomonas sp. UYIF39. Our findings enrich the existing catalog of biological parts, offering a repertoire of broad host-range promoter sequences that exhibit functionality across diverse members of the phylum Pseudomonadota, proving Antarctic microbial community as a valuable resource for prospecting new biological parts for synthetic biology.

Implementing Semantic Correlation Rules in the Domain of Energy Technology for Transportation Systems

This research explores the correlation between topics in a content delivery portal (CDP) and spare parts catalogue (SPC) instances, focusing the domain of energy technology for transportation systems. It is based on use cases that require further textual content in the CDP as well as reference links to corresponding instances in the SPC. Furthermore, this paper aims to show the process of creating Semantic Correlation Rules (SCR) – rule-based links between instances in a semantic network – based on the previously elaborated ontology. Moreover, it aims to assess, whether SCR meet users’ needs and discusses the strengths and difficulties of this implementation approach. In summary, this paper shows the general implementation process of SCR as well as the associated requirements in the field of energy technology for transportation systems.

A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae

The synthetic biology toolkit for baker's yeast, Saccharomyces cerevisiae, includes extensive genome engineering toolkits and parts repositories. However, with the increasing complexity of engineering tasks and versatile applications of this model eukaryote, there is a continued interest to expand and diversify the rational engineering capabilities in this chassis by FAIR (findable, accessible, interoperable, and reproducible) compliance. In this study, we designed and characterised 41 synthetic guide RNA sequences to expand the CRISPR-based genome engineering capabilities for easy and efficient replacement of genomically encoded elements. Moreover, we characterize in high temporal resolution 20 native promoters and 18 terminators using fluorescein and LUDOX CL-X as references for GFP expression and OD600 measurements, respectively. Additionally, all data and reported analysis is provided in a publicly accessible jupyter notebook providing a tool for researchers with low-coding skills to further explore the generated data as well as a template for researchers to write their own scripts. We expect the data, parts, and databases associated with this study to support a FAIR-compliant resource for further advancing the engineering of yeasts.

25 Years Ago July 1995

Competitiveness initiatives; manual/CNC technology’s benefits; Renishaw’s new machine shop impresses; TS Harrison & Sons’ Alpa is claimed unique; UK CNC units get support; 3D printing association; CAD-based parts catalogue

Maintenance Framework for Repairing Partially Damaged Parts Using 3D Printing

Additive manufacturing via 3D printing can enable the efficient and cost-effective replacement of damaged parts that can be produced at any manufacturing location and even when the parts are discontinued but their 3D CAD models are available. In addition, damaged portions of a part can be reconstructed by utilizing 3D printing. However, using a 3D printer requires a skilled operator with knowledge of this technology and other technical aspects. Hence, in this research, a user-friendly maintenance framework has proposed for any operator to repair partially damaged parts using 3D printing without requiring expert technical support. The framework includes a parts catalog with information necessary for 3D printing, a search module for automatically identifying damaged parts without prior knowledge about the part, and a shape comparison module for validation of the repaired part through damage detection and error measurement. Design and implementation of a maintenance support system based on the proposed framework are explained and the result of conducted experiment with a damaged ball from a valve to verify its performance is presented.

Constructions Of 10-Storey Residential Buildings With Steel Frame

Nowadays residential and civil buildings in our country are often erected by using the monolithic frame without collar beams. The disadvantages of this system are the result of the rejection of the basic principles of the national school of design, implemented in the 1970s in the method of a Single catalog of unified parts. This method is based on using prefabricated reinforced concrete constructions for savings of steel, reducing the labor input, erection time and cost of construction. The essence of the catalog system is a set of unified products and parts, from which buildings of different purposes are mounted with individual space-planning solutions, architecture, number of storeys, etc. À powerful scientific, personnel and production base, which made it possible to move to the industrial method of construction, was created under the method of a Single catalog in our country. Herewith, this system has replaced other structural solutions, first of all, the steel structures of frames. The latter have well-known advantages and are most consistent with the basic principles of design. Therefore, recently there has been an increasing interest in the use of steel frames. The evidence of using such constructions as a bearing system when constructing 10-storey residential buildings in Maykop is given in this article. Planning and constructive decisions of buildings, features of their realization, when using a braced framework with precast reinforced concrete panels of floor slabs and walls, are considered. The analysis of the stress-strain state of structures and indicators of steel consumption, including structural elements is carried out. It is also noted that the requirements of standards for strength and stiffness are met with a margin and the system has reserves for its improvement.

개인제조를 위한 부품 카탈로그 시스템 개발

This paper proposed a part catalog system for personal manufacturing that emerges recently based on personal fabrication machines, social media and a set of accessible engineering software. To support deficiency of personal manufacturing, the lack of human resources, it aims to integrate design and customer support from the view of product life cycle management. Extending the previous study on integration of design and manufacturing to customer support, it provides a parts catalog system that consists of a parts catalog editor and a viewer. The editor itself is an extended product data management (PDM) system that manages product data for design and customer support in an unified user environment. The viewer allows easy access of customers or customer support agents to the parts catalog through Web environment. The editor and viewer also provide social media that can directly connect personal manufacturers and customers to support efficiency of customer support in personal manufacturing environment. The proposed system was implemented as a prototype parts catalog system based on an existing research and education purpose PDM system. This paper also applied industrial example data to the implemented system to verify the feasibility of the proposed parts catalog system.

Book Review: The Diener: Poems

Diener: Poems. By Martha Serpas. Baton Rouge, La.: Louisiana State University Press, 2015. 78 pp. $17.95 (paper).Martha Serpas's new poetry book, draws on a pastoral tradition that associates obligations of Christian with caring for flocks in pastures. This tradition originated in Bible, which construed pastor according to its Latin definition; pastor originally meant or who provided food. When prophet Jeremiah declares, I will give you pastors according to mine heart, which shall feed you with knowledge and (Jer. 3:15 KJV), he makes use of ancient association of and shepherd. As if fulfilling Jeremiah's promise about pastors, Jesus in John's Gospel assumes role of the good shepherd who feeds his flocks with spiritual understanding and giveth his life for sheep (John 10:11 KJV). Having worked as a hospital chaplain, Serpas gravitates to this pastoral tradition, and as an environmental activist she stresses importance of actual pastures-and of natural world in general-that must be kept healthy to feed flocks.Serpas s book begins with an introductory poem, The Diener, which focuses on related concepts of health and wholeness, and way an individual's body, like world's body, is an ecosystem in which different parts must work together as a whole for there to be health. As director of a morgue, Serpas s is all too familiar, with parts breaking down and wholes dying and decomposing. If one endorses Serpas's proposition that God's image is (p. 1), which she asserts before giving a catalog of body parts, one should also endorse ideal of composite whole. We like to see ourselves as whole, she writes, diener piling legs on a cot / despite pruned artery, tied and cut (p. 1). Like Frost counseling his readers to drink and be whole beyond confusion with a makeshift communion goblet like Grail at end of Directive, Serpas throughout Diener directs those who find their lives in confusing fragments to pursue ideal of wholeness. At times, her urge to counsel makes her didactic. Education is answer / to our social woes, she says at start of Pearl Snap (p. 10), a poem drawing on her experiences as a hospital chaplain. Poems such as Badlands, in which a patient tattooed with wolves boasts he is an atheist, or Asperges, in which a nurses washes a dead baby boy whose green skin sloughs off // like bruised fruit skins (pp. …

The new German soundproofing standard: Comparison between DIN 4109:1989 and E DIN 4109:2013 / Die neue Schallschutznorm: Vergleich DIN 4109:1989 und E DIN 4109:2013

AbstractIn 2013, a new draft of a complete standard for DIN 4109 “Soundproofing in buildings” was issued in 4 parts – “Requirements”, “Method of calculation”, “Parts catalogue” and “Testing acoustics in buildings”. It includes several increases in the requirements for protection against airborne noise and impact sound by comparison to DIN 4109:1989. The increases are based on the grades from monitored buildings of standard construction periodically noted in recent years. Significant changes are also included in Part 2 “Input data for verifying the requirements by calculation”. A detailed computation of all transmission paths is carried out here, taking into account the special vibration reducing indices. For a brick wall, the specific indices for the weighted sound reduction index R’w,R are shown that arise for various formations of the joints. When compared with the calculated values according to DIN 4109:1989 there are differences of up to 10 dB.

Combinatorial Design Optimization of Automotive Systems by Connecting System Architecture Models with Parts Catalog

Combinatorial Design Optimization of Automotive Systems by Connecting System Architecture Models with Parts Catalog

How synthetic biology will reconsider natural bioluminescence and its applications.

As our understanding of natural biological systems grows, so too does our ability to alter and rebuild them. Synthetic biology is the application of engineering principles to biology in order to design and construct novel biological systems for specific applications. Bioluminescent organisms offer a treasure trove of light-emitting enzymes that may have applications in many areas of bioengineering, from biosensors to lighting. A few select bioluminescent organisms have been well researched and the molecular and genetic basis of their luminescent abilities elucidated, with work underway to understand the basis of luminescence in many others. Synthetic biology will aim to package these light-emitting systems as self-contained biological modules, characterize their properties, and then optimize them for use in other chassis organisms. As this catalog of biological parts grows, synthetic biologists will be able to engineer complex biological systems with the ability to emit light. These may use luminescence for an array of disparate functions, from providing illumination to conveying information or allowing communication between organisms.

Reconstrucción virtual del mobiliario doméstico romano a través de las fuentes antiguas

<p>The aim of this paper is the recreation of the various domestic environments of the Romandomus, with particular attention to the furniture and dishes that are linked very closely with each one of the rooms within it. First, there has been a collection of ancient sources, with particular attention to the inscriptions, wall paintings and mosaics, then there has been a catalog of parts in response to various aspects such as the type of object or stay they occupied.Secondly we have proceeded to the digitization of images for late reconstruction using SketchUp8. In conclusion, we intend to virtually rebuild the Roman household furniture to make it understandable to these spaces.</p>

The Poet as Translator: Mary Wortley Montagu Approaches the Turkish Lyric

The Poet as Translator: Mary Wortley Montagu Approaches the Turkish Lyric

The National Shipbuilding Research Program Contribution in Advancing Information Technology in the Shipbuilding Enterprise

The purpose of the National Shipbuilding Research Program is to apply information technologies that can reduce the cost of building and maintaining US Navy warships. The program targets solutions to consensus priority issues that exhibit a compelling business case to improve the efficiency of the US Shipbuilding and Ship Repair Industry and involves US shipyards, academia, technology companies, and government agencies. The projects are funded by the government with equal cost shared by project participants. The Systems Technology area projects involve all portions of the industry and cover a wide array of projects including: Product Information Modeling, Business Process Technologies, Common Parts Catalog (CPC), Automatic Generation of Control Program for Robotic Welding, Shipyard Design Tool Enhancement, Capturing As-Built Models For 3D CAD Systems, Shipyard Equipment Wireless Monitoring And Control, Open Architecture Standards, and Other Shipyard Computer Applications.

Meta-ontology for automated information integration of parts libraries

Seamless integration of digital parts libraries or electronic parts catalogs for e-procurement is impeded by semantic heterogeneity. The utilization of ontologies as metadata descriptions of the information sources is a possible approach to providing an integrated view of multiple parts libraries. However, in order to integrate ontologies, the mismatches between them should be resolved. In this paper, we propose meta-concepts with which the ontology developers describe the domain concepts of parts libraries. The meta-concepts have explicit ontological semantics, so that they help to identify domain concepts consistently and structure them systematically. Consequently, our method ensures that the mismatches between parts library ontologies are confined to manageable mismatches which a software program can resolve automatically. Modeling ontologies of real mold and die parts libraries is taken as an example task to show how to use the meta-concepts. We also demonstrate how easily a computer system can merge the resultant well-established ontologies.

The One Shipyard in Practice: Implementation of a Second-Tier Common Parts Catalog

This paper presents the methodology for the realization of a Common Parts Catalog (CPC) system for second-tier shipyards. Applying current principles in data management and conceptualization, a relational system is employed whereby the previously unstructured and haphazard approach to material management and parts definition is broken down to a meaningful and systematic taxonomic structure. The current majority of second-tier shipyards employ antiquated and/or bulky control systems for tracking and monitoring parts within each shipyard's own cataloging systems. This has led to a spate of data that are often duplicated needlessly, as well as leaving the most current state of the catalogs in such disarray as to be almost indecipherable in some instances. For these reasons, parts catalogs have often had to be "reinvented" as they are needed. Unfortunately, the process was often not undertaken with either a full understanding of database management and creation or with any meaningful level of standardization in the data. Given the latest advances in computing technology and the decreasing cost of associated hardware to utilize these technologies, a means of standardizing and organizing a shipyard's parts catalog has become a crucial step in the further development of the industry as a whole. From the extremely outdated method of parts tracking and cataloging on paper, to the latest available computer hardware and relational databases, the CPC aims to alleviate the shipyards of the tasks involved with maintaining a disparate collection of data used currently as a parts cataloging system and to replace it with a highly organized and flexible data structure to store and manage parts and materials. The National Shipbuilding Research Program's (NSRP) Integrated Shipbuilding Environment (ISE) project standardized and classified the methods of catalog construction and administration such that needless replication and unwieldy catalog size have all but been eliminated. These same ISE type Common Parts Catalog standards and procedures are now being adopted by second-tier shipyards in an attempt to better utilize resources available, streamline the process of cataloging parts and material, and facilitate direct interaction among shipyards' (both first and second-tier) catalog systems.

Potential Roles for Diatomists in Nanotechnology

Diatoms produce diverse three-dimensional structures that, due to their exponential rate of growth, may be of use in the manufacture of components for nanotechnology as an alternative to present linear lithographic techniques. Vapor replacement of the silicon permits the conversion of diatom silica valves and other structures to metal/ceramics, with no loss of structure. The literature on diatom nanotechnology is reviewed, along with suggestions on how diatomists might enhance this emerging technology. There is a need for a systematics based catalog of parts (via genomics technologies), improved diatom culture techniques, better understanding of the mechanisms of diatom morphogenesis and motility, and genetic manipulation, mutagenesis, and selection, as via the chemostat-like compustat. Given the self-motility of raphid diatoms, they could form the basis for industrially useful nanobots.

From parts catalog to wiring diagram

From parts catalog to wiring diagram

Advances in flux balance analysis.

Biology is going through a paradigm shift from reductionist to holistic, systems-based approaches. The complete genome sequence for a number of organisms is available and the analysis of genome sequence data is proving very useful. Thus, genome sequencing projects and bioinformatic analyses are leading to a complete 'parts catalog' of the molecular components in many organisms. The next challenge will be to reconstruct and simulate overall cellular functions based on the extensive reductionist information. Recent advances have been made in the area of flux balance analysis, a mathematical modeling approach often utilized by metabolic engineers to quantitatively simulate microbial metabolism.

WDS 3D parts catalogue with 10,000 parts on-line

WDS 3D parts catalogue with 10,000 parts on-line