Substrate Particle Size Research Articles

Characteristics of the macroinvertebrate community structure and their habitat suitability conditions in the Chishui River

IntroductionAs an important tributary of the upper Yangtze River without dams, the study of the macroinvertebrate community structure and habitat suitability conditions in the Chishui River holds significant implications for water ecological conservation and restoration.MethodsIn order to explore the species composition, community characteristics and their ecological needs of macroinvertebrates in the Chishui River, 26 sampling sites were deployed in the Chishui River in this study in March (dry season), July (wet season), and November (normal season) of 2023 and performed community structure analysis.ResultsA total of 153 macroinvertebrate taxa were identified, with 62 taxa recorded in the dry season, 46 in the wet season and 115 in the normal season. The assemblage was predominantly composed of aquatic insects from the EPT group (Ephemeroptera, Plecoptera and Trichoptera). The results of ANOSIM analysis indicate that the species composition of macroinvertebrates varies significantly across different water periods. The Shannon-Wiener diversity index and Margalef index were significantly higher during the normal season compared to the dry and wet seasons. In contrast, the Pielou index and Simpson index remained relatively stable, suggesting a higher level of evenness in community structure across the different water periods. Based on the habitat suitability curves, Corbicula fluminea and Heptagenia prefer environments with high flow velocities and substrate particle sizes (D50) smaller than 300 mm. Hydropsyche sp.1 and Baetis thrive in environments with medium to low water depths, high flow velocities, and D50 ranging from 100 to 300 mm. Caridina favor deeper waters, low flow velocities, and larger substrate particles. The GAM fitting results revealed that the number of macroinvertebrate taxa increased with rising pondus hydrogenii (pH) and electrical conductivity (EC) levels. Species richness initially declined and then rose within certain thresholds of total nitrogen (TN) and total phosphorus (TP) concentrations. The optimal conditions for macroinvertebrate communities in the Chishui River were determined to be a DO concentration of 9.8 mg/L and an NH3-N concentration of 0.12 mg/L.DiscussionOverall, the macroinvertebrate community structure in the Chishui River is significantly influenced by factors such as water quality, flow velocity, and substrate particle size, and it demonstrates strong adaptability to seasonal variations.

Influence of substrate particle size and detention time on mycelium-colonized sawdust efficiency for removal of faecal bacteria from slaughterhouse wastewater in batch treatment

Mycofiltration is a recent cost-effective biotechnology that is still under development for wastewater treatment. The use of mycelium-colonised substrate for wastewater treatment in a batch system with delayed residence time and the effect of sawdust particles has not previously been considered. Slaughterhouse wastewater is discharged untreated in many developing countries majorly due to the high cost of existing conventional treatment systems. The effect of sawdust detention time and particle size on the removal efficiency of faecal bacteria from slaughterhouse wastewater by Pleurotus ostreatus mycelium was assessed in the lab using mycelium colonized with sawdust of particle sizes (0.6, 1.18, 2.36 mm and unsorted particle sizes) and under varying detention times (0, 12, 24, 36, 48, 60 and 72 hours) using batch treatment procedure. Hydrogen peroxide produced during mycelium colonisation of the sawdust was also evaluated. Oxidation-reduction potential (ORP) was measured during this study to determine the oxidative capacity in each treatment reactor. The removal efficiency of Escherichia coli ranged from -7.9 – 77.2 % and was the highest for mycofilter with 2.36 mm sawdust particle size at 72 hours detention time (0.7 log removal). Salmonella spp. removal efficiency ranged from 0.66 – 71 % with the highest efficiency recorded in the system with 1.16 mm also at 72 hours (0.4 log removal). Mean hydrogen peroxide concentration ranged from 0.53±0.12 (unsorted inoculated) to 25.18±1.77 mg/l (1.18 mm, 72 hours). ORP values ranged from 5.0±2.2 mV (raw wastewater, 24 hours) to 232±55 mV (unsorted inoculated, 72 hours). The result of this study showed that substrate particle size and detention time have roles to play in the efficiency of mycofilters using batch treatment. The concentration of hydrogen peroxide was also influenced significantly by sawdust particle size. Therefore, there is a need to further study this system in a bid to optimize its ability to remove faecal bacteria from wastewater.

Determination of Particle Size for Optimum Biogas Production from Ouagadougou Municipal Organic Solid Waste

Anaerobic digestion’s contribution to sustainable development is well established. It is a sustainable production process that enables energy to be saved and produced and efficient pollution control processes to be implemented, thereby contributing to the sustainable development of our societies. Optimizing biogas yields from the anaerobic digestion of municipal organic waste is crucial for maximum energy recovery and has become an important topic of interest. Substrate particle size is a key process parameter in biogas production and precedes other pretreatment methods for most organic materials. This study aims to evaluate the impact of particle size and incubation period on biomethane production from municipal solid waste. Sampling of municipal solid waste was carried out in waste pre-collection in the city of Ouagadougou, Burkina Faso. Waste characterization showed lignocellulolytic green waste (grass, dead leaves), waste composed of fruit and leafy vegetables and leftover food waste. TableCurve 3D v4.0 software was used to develop an optimal mathematical model to correlate particle size and biomethane productivity to describe optimal production parameters. Particle sizes ranging from 2000 to 63 µm high biogas production values, specifically 385.33 and 201.25 L·kg−1 of MSV. PCA analysis clearly showed a high correlation between particle size and biogas production, with optimum production recorded for size 250 µm with a biomethane production value of 187.53 L·kg−1 of MSV. The average relative errors and RMSE for CH4 content were improved by 24.31% and 44.97%, respectively. The data calculated with the developed mathematical model and the existing experimental data were compared and permutated to validate the model. This work enabled the identification of a mathematical model that describes the correlations between the input parameters of an experiment and the monitored parameters, as well as the definition of the particle size that allows for the optimal production of biomethane.

Pattern formation on hydrophobic PDMS substrates by evaporating droplets: combined effect of substrate stiffness, relative humidity and particle size

Desiccation patterns left by micro-droplets of water impregnated with particles on hydrophobic substrates have been analyzed with respect to variations in the elastic stiffness of the substrates, particle size and relative humidity. The complex and unique patterns obtained, have been analyzed and explained in terms of the time scales of moving Triple Phase Line (TPL) on substrate and substrate relaxation rate. The rate of TPL movement is found to depend on the relative humidity and substrate stiffness. In turn, this affects the contact angle hysteresis. Particle movement is a result of viscous drag and inertia apart from electrostatic interactions. We have successfully explained the myriad patterns obtained from drying droplets via systematic rheological measurements along with an understanding of the role of all the effective forces and their time scales of action.

Fluvial habitat associations of riverine dragonflies (Odonata, Gomphidae) in the Huron Mountains (Michigan, USA)

AbstractThe presence of sediment‐burrowing clubtail dragonflies (Gomphidae) may be directly related to riverine geomorphic properties. Their synchronous emergence behavior, marked by persistent exuviae, allows for the examination of emerging adult‐stage habitats. Here we explore how emerging adult and benthic larvae are related to hydrogeomorphic factors through regression modeling. Nine sites in two subwatersheds of the Huron Mountain region (Michigan, USA) were surveyed during two periods: May/June 2021 and August 2021. Temperature, pH, dissolved oxygen, conductivity, and discharge were measured concurrent with invertebrate surveys, and field samples of total suspended solids, nutrients, and substrate particle size distribution were also taken. In spring, 317 exuviae were collected, including 153 Gomphidae. In August, 45 larvae were collected including 22 Gomphidae. Spearman's rank correlations preceded robust regression analysis to aid variable selection. Although nonsignificant, Gomphidae exuviae were negatively correlated with conductivity, average water depth, and percent sand while non‐Gomphidae exuviae were positively correlated with the same variables. The model selection found the top models for Gomphidae and non‐Gomphidae exuviae abundance to relate to depth and conductivity, while the top model for Gomphidae larvae was with discharge. All single variable models (discharge, width, and percent sand) had similar AICc criterion values when examining their relationship with non‐Gomphidae larval abundance. Our study demonstrates that larvae of several riverine dragonfly taxa in the Huron Mountains co‐occur despite hydrogeomorphic variation, yet, at emergence, specific taxa may be responding to different stream properties. Understanding the habitat requirements of riverine dragonflies and how these potentially shift throughout development can support conservation efforts.

Characteristics of surface flow field and substrate in the spawning ground of Schizothorax grahami

The Spawning ground is a crucial habitat for fish, and the physical characteristics of the habitat are important for fish life history. In this paper, Large-Scale Particle Image Velocimetry (LSPIV) was used to measure the surface flow field and the substrate (particle size and mineral composition) in the spawning ground of Schizothorax grahami. Based on the literature and consideration of experimental feasibility, two spawning grounds in the source of the Chishui River were selected—Erlongqiangbao (located in the mainstream) and Bamaoba (located in the tributary), two and three cross sections of each river section were analyzed respectively. The results show that Erlongqiangbao first flows in the direction of “southeast-northwest” and then “southwest—northeast” after crossing the bridge; the maximum velocity of the reach is 3.374m/s. The size of the substrate is mainly less than 1mm in the upstream section of Erlongqiangbao, both 25-50mm in the middle and downstream, and the substrate type mainly is quartz and calcite; Gushangtian flows in the direction of “southwest-northeast,” the maximum velocity of the reach is 1.537m/s, the size of the substrate is mainly greater than 50mm in the upstream, and 25-50mm in the middle and downstream, the substrate type mainly is quartz, calcite, and dolomite. The mineral compositions of the two spawning sites were analyzed by principal component analysis. Quartz and anorthose are more than 1 in composite scores, respectively ranked first and second, indicating that they play a dominant role in the mineral composition. This study could provide basic data and ideas for restoring S. grahami spawning ground.

An Initial Survey of Unionid Mussels in Lakes East of the Missouri River in South Dakota, USA

This study surveyed freshwater mussels (family Unionidae) in 116 lakes and reservoirs east of the Missouri River in South Dakota, USA, during 2017. Using two-person–hour/site timed searches, evidence of a total of 1789 mussels, including 1053 live mussels, was obtained from 50 waters. Nine species, from two different orders, were found in lakes and reservoirs throughout five of the six major river drainages east of the Missouri River. The native species observed included Giant Floater Pyganodon grandis, Fatmucket Lampsilis siliquoidea, Threeridge Amblema plicata, White Heelsplitter Lasmigona complanata, Wabash Pigtoe Fusconaia flava, Deertoe Truncilla truncata, and Pink Heelsplitter Potamilus alatus. Giant Floater was the most widespread and abundant species observed, representing 63.3% of the live mussels sampled. Two non-native species, Zebra Mussel Dreissena polymorpha and Chinese Basket Clam Corbicula fluminea, were also documented from three water bodies in the lower Missouri River drainage. Overall, mussel abundance was negatively correlated with lake water conductivity and positively correlated with turbidity. No significant correlations were observed between species abundance and water temperature, pH, dissolved oxygen, or substrate particle size.

Rice Husk, Brewer's Spent Grain, and Vine Shoot Trimmings as Raw Materials for Sustainable Enzyme Production.

Solid by-products with lignocellulosic structures are considered appropriate substrates for solid-state fermentation (SSF) to produce enzymes with diverse industrial applications. In this work, brewer's spent grain (BSG), rice husk (RH), and vine shoot trimmings (VSTs) were employed as substrates in SSF with Aspergillus niger CECT 2088 to produce cellulases, xylanases, and amylases. The addition of 2% (NH4)2SO4 and 1% K2HPO4 to by-products had a positive effect on enzyme production. Substrate particle size influenced enzyme activity and the overall highest activities were achieved at the largest particle size (10 mm) of BSG and RH and a size of 4 mm for VSTs. Optimal substrate composition was predicted using a simplex centroid mixture design. The highest activities were obtained using 100% BSG for β-glucosidase (363 U/g) and endo-1,4-β-glucanase (189 U/g), 87% BSG and 13% RH for xylanase (627 U/g), and 72% BSG and 28% RH for amylase (263 U/g). Besides the optimal values found, mixtures of BSG with RH or VSTs proved to be alternative substrates to BSG alone. These findings demonstrate that SSF bioprocessing of BSG individually or in mixtures with RH and VSTs is an efficient and sustainable strategy to produce enzymes of significant industrial interest within the circular economy guidelines.

Humic acid biosynthesis and bacterial community evolution during aerobic composting of rice straw

In this study, the effects of inoculum ratio, substrate particle size and aeration rate on humic acid (HA) biosynthesis during aerobic composting of rice straw were investigated, respectively. The contents of total organic carbon, total nitrogen and HA, as well as lignocellulose degradation in the composting were evaluated, respectively. It is found that the maximal HA yield of 356.9 g kg−1 was obtained at an inoculum ratio of 20%, a substrate particle size of 0.83 mm and an aeration rate of 0.3 L·kg−1 DM min−1 in the process of composting. The changes of microbial communities and metabolic functions at different stages of the composting were also analyzed through high-throughput sequencing. The result demonstrates that Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were the dominant phyla and their relative abundance significantly varied over time (p < 0.05), and Rhizobium, Phenylobacterium, Pseudoxanthomonas and Paenibacillus were positively related to HA content in the compost. Furthermore, the metabolic function profiles of bacterial community indicate that these functional genes in carbohydrate metabolism and amino acid metabolism were involved in lignocellulose biodegradation and HA biosynthesis. This work may be conducive to explore new regulation strategy to improve bioconversion efficiency of agricultural residues to applicable biofertilizers.Key points• Temperature, pH, TOC, TN and C/N caused a great influence on humic acids synthesis• The succession of the microbial community during the composting were evaluated• The metabolisms of carbohydrate and amino acids were involved in HA synthesisGraphical abstract

Taxonomic richness and its relationship to the functional diversity of polychaetes in tropical estuaries

AbstractBiological diversity can be evaluated by the taxonomic and functional components. This study aimed to assess the relationship between taxonomic richness with the functional diversity components [functional richness (FRic), functional evenness (FEve) and functional divergence (FDiv)] and the functional trait dominance of polychaetes. In addition, we evaluated the environmental parameters that shape the functional structure in six tropical estuaries in north‐eastern Brazil. Three estuaries have a continuous environmental gradient (typical tropical), and the other three estuaries have no defined environmental gradient (semiarid tropical). We identified significant differences in the dominance of feeding strategy and habitat between estuaries, demonstrating that the functional space of the communities is not equally occupied. Also, the substrate particle size composition was correlated with the functional structure. FRic showed a positive relationship with taxonomic richness, regardless of the type of estuary. As taxonomic richness increases, the probability of different combinations of functional categories added to a community also increases. FEve and FDiv seem to be independent of taxonomic richness. Therefore, it is important to simultaneously evaluate diversity's taxonomic and functional components, considering the complementarity of the information provided by these diversity measures.

The Dewatering Performance of a Compact Screw Press Manure Separator for Non-Typical Substrates

The dewatering of substrates is one of the key tasks in the handling of material flows in a circular economy. Besides belt filters, sedimenters, and decanters, screw presses are frequently used to reduce the water content of substrates. However, screw presses available on the market are usually designed for high throughputs (&gt;5 m3/h). The dewatering of smaller feed rates (&lt;1 m3/h), however, requires more compact solutions. For this reason, a compact screw press (short screw length), which is normally used in agriculture for cow manure dewatering, was investigated in this work, as there exist no publications on this type of compact screw press. A mobile test setup was developed and loaded with different material such as digested sludge from a wastewater treatment plant, commercially available potting soil, wood shavings, and biological waste. The dry matter content of the feed material, the feed material itself, the screw rotational speed, and the weight setting (pressure) at the back plate were varied, and the effects on the dewatering result were recorded. A significant influence on the dewatering result was found for the weight setting, the type of substrate (particle size), and the dry substance content of the feed material. The tests confirmed that the compact screw press separator is suitable for the dewatering of wood shavings and biological waste (with an increase in dry matter percentage of up to 7.7%). The direct dewatering of potting soil and digested sludge is not possible and can only be implemented by using structural support material. This study fills the gap between published work on large screw presses and potentially new application fields of compact screw press manure separators, which, until now, have only been used in agriculture.

Kinetic Modeling of Anaerobic Co-Digestion Of Plant Solid Waste with Sewage Sludge: Synergistic Influences of Total Solids and Substrate Particle Size in Biogas Generation

Kinetic Modeling of Anaerobic Co-Digestion Of Plant Solid Waste with Sewage Sludge: Synergistic Influences of Total Solids and Substrate Particle Size in Biogas Generation

Seed mucilage as a defense against granivory is influenced by substrate characters.

Many seeds are consumed by granivores despite numerous adaptations to prevent detection or exploitation. The environment can influence the efficacy of these defensive traits. Understanding the mechanisms by which environmental factors modify defensive efficacy is important for understanding spatial patterns of granivory and seed recruitment. Seed mucilage is a sticky coating that binds imbibed seeds to substrates; this attachment has been demonstrated to lessen exploitation by granivores. Seed mucilage as a defense has been recognized for decades, though rarely studied. Here, we investigated whether the environment alters this seed defense by addressing two questions: (1) Does substrate particle size affect attachment strength? (2) Does a change in particle size lead to changes in granivore-related mortality? In the field experiment, ants removed more seeds from finer substrates than their coarser counterparts. Across that same grit range, seeds took less force to dislodge when mucilage-bound to fine sandpaper; however, an investigation across a wider range of grits demonstrated nonlinearities occurred for many species, probably due to structural and chemical mucilage properties. Small differences in substrate grit lead to differential mortality in mucilaginous seeds due to alterations in attachment strength, suggesting that the defensive efficacy of this trait differs across the landscape. This work paves the way for a more integrative look at mucilaginous seeds. Seed mucilage is a widespread trait that is easily studied and has important demographic implications. It represents an ideal system to examine dispersal, germination, and granivory to gain a more holistic view of seed ecology.

Leaf yield and mineral content of mizuna in response to cut-and-come-again harvest, substrate particle size, and fertilizer formulation in a simulated spaceflight environment

The Veggie plant-growth unit deployed onboard the International Space Station (ISS) grows leafy vegetables to supplement crew diets. “Cut-and-come-again” harvests are tested to maximize vegetative yield while minimizing crew time. Water, oxygen, and fertilizer delivery to roots of leafy greens growing in microgravity have become the center of attention for Veggie. Current Veggie technology wicks water into particulate root substrates incorporating controlled-release fertilizer (CRF). Mizuna mustard (Brassica rapa) was grown under ISS-like environments in ground-based Veggie-analogue units comparing crop response to combinations of two different substrate particle sizes, two different fertilizer formulations, and three leaf-harvest times from each plant. Fine-particle porous ceramic substrate (Profile©) was compared with a 40:60 mix of fine-particle porous ceramic Profile© + coarse porous ceramic Turface© substrate. Identical 18-6-8 (NPK) CRF formulations consisting of [50% fast-release (T70) + 50% intermediate-release (T100) prills] vs. [50% fast-release (T70) + 50% slow-release (T180) prills] were incorporated into each substrate, and leaf tissues were harvested from each treatment combination at 28, 48, and 56 days after sowing. The combination of T100 CRF in 100% Profile© substrate gave the highest fresh mass (FM) and leaf area (LA) across harvests, whereas T180 CRF in 40% Profile© gave the lowest. Dry-mass (DM) yields varied with effects on leaf area. Tissue nitrogen (N) and potassium (K) specific contents declined across harvests for all treatment combinations but tended to be highest for T100 CRF/100% Profile©, and lowest for T180 CRF/40% Profile©. These major macronutrients were taken up faster by roots growing in small particle-size substrate incorporating intermediate-rate CRF, but also were depleted faster from the same treatment combination, suggesting it may not continue to be the best combination for additional harvests. Micronutrients did not decline in tissue specific content across treatment combinations, but manganese (Mn) accumulated in leaf tissue across treatments and apparently comes mainly from the ceramic substrate, regardless of particle size. Substrate leachate analysis following final harvest indicated that pH remained in the range for nominal availability of mineral nutrients for root uptake, but electro-conductivity measurements suggested depletion of fertilizer salts from root zones, especially from the treatment combination supporting the highest yields and major macronutrient contents. Although 100% Profile© was the better growth substrate for mizuna in combination with intermediate-rate CRF and three cut-and-come-again harvests in ground-based studies, mixed-particle-size substrates may be a better choice for plant growth under microgravity conditions, where capillary forces predominant and tend to cause saturation of a fine medium with water. Since there were no statistically significant interactions between substrate and fertilizer in this study, our ground-based findings for CRF choice should translate to the best substrate choice for microgravity, but if NASA wants to consider additional cut-and-come-again harvests from the same mizuna plants, more complex CRF formulations likely will have to be investigated.

Temporal Changes in Water Quality with Increasing Ambient Temperatures Affect the Distribution and Relative Abundance of 10 Species of Balitorid Fishes in Small Streams of Eastern Thailand

Stream degradation increases with high anthropogenic activity and climate variability, while declines occur in biodiversity. However, few studies have been undertaken on tropical waterways, a major impediment to biodiversity conservation. The present study was conducted on 95 relatively pristine small streams in Eastern Thailand with 10 reasonably uncommon species of balitorid fishes. Measurements were made of 21 physical and chemical factors and the substrate particle size. Stepwise regression identified the direct importance of substrate particle size and nitrate on the species’ richness of balitorids, whereas its abundance was negatively related with iron concentrations. A Canonical Correspondence Analysis identified three fish groups: the 1st group was negatively correlated with ammonia and positively correlated with dissolved silica, the 2nd group was positively correlated with substrate particle size and negatively correlated with stream ambient temperature and ammonia concentration, and the 3rd group was negatively correlated with low dissolved silica, respectively. The results of this study may indicate the vulnerability of balitorids under climate warming and anthropogenic pressure that alter the water physicochemical factors and river degradation including the substrate type. Thus, a conservation framework should be provided regarding the limits for water temperature, ammonia, and iron in Thailand’s Water Quality Criteria to better protect its freshwater ecosystem. Balitorid is a potential bioindicator for evaluating the river temperature effect in combination with ammonia nutrient stressors as long as the way-of-life habits of the species are taken into account.

Using swim‐up traps to assess Arctic charr (Salvelinus alpinus) spawning habitat and the phenology and density of emergent fry

AbstractThe Arctic charr (Salvelinus alpinus) is a species of cultural, economic and conservation importance, but hitherto, investigations of critical early life stages have been few. Here, at a lake in the United Kingdom, we used swim‐up traps to investigate the phenology of fry emergence and associations between fry density and habitat. The first emergence occurred on 4 or 5 March 2020 and 2021, with numbers peaking and remaining stable in the following 2 weeks. Emergence in 2021 had finished by 27 March but on the same date in 2020 emergence was ongoing when COVID‐19 ended sampling. Substrate particle size ranged 31–94 mm and was negatively correlated with fry density. Likewise, density was negatively correlated with water depth and aquatic plant cover, but there was no relationship with flow velocity. Traps were effective and non‐destructive for assessing the location and productivity of spawning sites for this locally threatened species.

Analysis of wellbore clogging by asphaltene deposition using interaction energies

Well columns could be prone to deposition of asphaltene with severe detrimental impacts. The goal of the current work was to determine how asphaltene particles that interact with casing surfaces may clog the well columns. Objectives such as whether asphaltene deposition forms at a certain depth of the wellbore and also the impact of particle size, production rate, and casing material on the probability of wellbore clogging have been considered. To do so, the extended Derjaguin-Landau-Verwey-Overbeek (XLDVO) interaction theory and surface properties were investigated in relation to the depth of well column based on measurements of the contact angle for interacting asphaltene-crude oil-casing systems. The findings of this investigation revealed that it is possible to determine if asphaltene deposition takes place at a specific depth of the well column. Additionally, the sticking probability of asphaltene particles was used to statistically and qualitatively predict the impact of casing substrate, production rate, and asphaltene particle size on the well column's clogging profile. The results indicate that when production rate and asphaltene particle size increase, a thinner layer of asphaltene deposits would be expected. Accordingly, the clogging risk decreased at least 60% with a three-fold increase in particle size from 500 nm to 1.5 µm.

Persistence of Antibiotic Resistance Genes Varies with Particle Size and Substrate Conditions in Recirculating Streams.

Antibiotic resistance (AR) determinants are enriched in animal manures, a significant portion of which is land-applied as a soil amendment or as fertilizer, leading to potential AR runoff and microbial pollution in adjacent surface waters. To effectively inform AR monitoring and mitigation efforts, a thorough understanding and description of the persistence and transport of manure-derived AR in flowing waters are needed. We used experimental recirculating mesocosms to assess water-column removal rates of antibiotic resistance genes (ARGs) originating from a cow manure slurry collected from a dairy farm. We quantified the effect of three benthic (i.e., bottom) substrate variations and particle sizes of manure slurry on water column removal rates. Overall, we observed variation in ARG behavior across substrate treatments and particle sizes. For ARGs associated with small particles, removal rates were higher in mesocosms with a substrate. tetW was typically removed at the highest rates across particle size and treatment, followed by ermB and blaTEM. Our data suggests that both substrate character and particle size exert control on the fate and transport of ARGs in surface waters, laying the foundation for future research in this area to establish a predictive framework for AR persistence and fate in flowing waters.

Box-Behnken design optimization of xylanase and cellulase production by Aspergillus fumigatus on Stipa tenacissima biomass

ABSTRACT Optimization of xylanase and cellulase production by a newly isolated Aspergillus fumigatus strain grown on Stipa tenacissima (alfa grass) biomass without pretreatment was carried out using a Box-Behnken design. First, the polysaccharides of dried and ground alfa grass were characterized using chemical methods (strong and diluted acid). The effect of substrate particle size on xylanase and carboxymethylcellulase (CMCase) production by the selected and identified strain was then investigated. Thereafter, experiments were statistically planned with a Box-Behnken design to optimize initial pH, cultivation temperature, moisture content, and incubation period using alfa as sole carbon source. The effect of these parameters on the two enzyme production was evaluated using the response surface method. Analysis of variance was also carried out, and production of the enzymes was expressed using a mathematical equation depending on the influencing factors. The effects of individual, interaction, and square terms on production of both enzymes were represented using the nonlinear regression equations with significant R 2 and P-values. Xylanase and CMCase production levels were enhanced by 25% and 27%, respectively. Thus, this study demonstrated for the first time the potential of alfa as a raw material to produce enzymes without any pretreatment. A set of parameter combinations was found to be effective for the production of xylanase and CMCase by A. fumigatus in an alfa-based solid-state fermentation.

Vermicomposting of solid textile sludge spiked with cow dung by epigeic earthworm Eisenia fetida in variable population density

AbstractA six‐week study was conducted on vermicomposting of textile sludge spiked with cow dung at a dry mass ratio of 2:3 by epigeic earthworm Eisenia fetida, with variable population densities (40, 80, and 120 earthworm/kg dry substrate). The earthworm characteristics and metal content of the substrate were observed at the beginning and the end of the experiment, whereas particle size, pH, electrical conductivity (EC), volatile matter (VM), total organic carbon (TOC), and total Kjeldhal nitrogen (TKN) were determined at every week. No mortality of E. fetida was found during the composting process. A high rate of cocoon or egg production (~4 cocoon earthworm−1) was evident in the most populated substrate. However, overcrowding affected the production of hatchlings, resulting in 5.57–1.03 hatchling earthworm−1 from the lowest to the highest population densities. Growth of the adult earthworm was also retarded, which varied from 0.44 to 0.35 g earthworm−1 with the increase of population density due to high competition for food. The particle size of the substrate material was reduced from 4 mm to 0.6 mm in the most populated substrate which was ascertained to be the most efficient option to reduce substrate particle size. All final substrates gained a C/N ratio lower than 20 except the lowest populated substrate. The change in metallic contents incorporated in substrates showed reverse relationship with the population. Though the overall result demonstrated that the physicochemical properties of the substrate materials are favored by the higher population density, it showed negative effect on the biological activities of earthworms.