Partial Submergence Research Articles

Novel Rice (Oryza sativa L.) Genotypes Tolerant to Combined Effect of Submergence and Salt Stress

The implications of both complete and partial submergence with saline water on growth and survival of different rice genotypes were investigated. The study showed that among the 199 genotypes, only five genotypes namely IC-459744, IC-469712, IC-464746, IC-459649 and IC-17024 were tolerant to both complete (plants under water) and partial submergence (water depth 45±5 cm) with saline water (12 dS m−1). The association of Chlorophyll a, Chlorophyll b, total Chlorophyll, normalized difference vegetative index (NDVI) and photochemical reflectance index (PRI) with score under partial submergence with salt stress were highly negatively signifi cant (p<0.001***). Biplot derived from principal component analysis showed that accessions such as IC-459744 and IC-459649, tolerant to both complete and partial submergence with salty water were in the same quarter where PRI was located with long vertex. The genotypes identified in this investigation have great potential as unique genetic resources for utilization in crop improvement programmes.

Submergence Tolerance in Irrigated and Rainfed Lowland Rice Varieties: Agro-Morphological Basis for Rice Genetic Improvement in Southern Benin

Objective: The study was carried out, on the rice perimeter of Southern Benin, to evaluate the response of irrigated and lowland rice varieties to the complete and partial submergence using agro-morphological traits. Method: Thus, 15 rice varieties were submerged in the field during 60 days according to split plot experimental design with two factors and three replications. Agro-morphological parameters such as plant height, number of tillers, the distance of internodes and the number of internodes were measured respectively at 13 days and 45 days. After harvest, some traits such as length, width and thickness of grains, panicle length, number of primary and secondary branches, the average number of empty grains and full grains per panicle and 1000-grain weight were recorded. Results: Very highly significant (P ≤ 0.0001) differences were identified between agro morphological parameters according to the type of submergence while the factor type of immersion has significant influence on the number of internodes at 45 days after transplanting (P ≤ 0.01). The submergence was not significant effect on tillering and length of internodes. Conclusion: The plant height, the weight of thousand grains and the average number of empty grains per panicle constitute key aspects and have been essential for evaluating tolerance to submergence in Benin.

Instability Criteria for Vehicles in Motion Exposed to Flood Risks

Flooded roads have somewhat become a norm to the society and among the damages that floods can pose, there are fatalities and harm caused to people. Floating debris such as vehicles, manipulated by floodwaters could potentially cause harm not only to the public safety but also towards the public and private-owned properties. In the past, research on vehicle’s instabilities have been solely dedicated to static vehicles which are normally translated as vehicles parked on road surface. A vehicle when exposed to floodwater get influenced by different hydrodynamic forces and becomes prone to different instability modes, namely sliding, floating and toppling. Outcomes on such modes are somehow recognised in the works on static vehicles, but the mechanics of a moving vehicle under such influences have not been studied. Herein the influence of floodwater flows on the vehicle attempting to cross a flooded path (partial submergence) is presented. With that regards, a non-stationary model vehicle with the scale ratio of 1:10 (Perodua Viva) was used and a series of experiments were conducted. Moreover, a new formula to estimate the incipient velocity for a moving vehicle has been introduced and the prediction accuracy of the proposed formula has been validated using experimental data. Measurements were taken including approaching velocities and water depths, through which the instability was computed.

Photocatalytic antimicrobial films on fluorinated contact lens polymers

Flat TiO2 thin films were dip coated on contact lens polymer substrates (fluorinated ethylene propylene copolymer, FEP) using a hybrid sol-gel and annealed for 12 h at ≤200 °C. The grain sizes were in the range 10–20 nm but extensive shrinkage cracking was present. The crystallinities of the films annealed at ≤150 °C increased with increasing temperature gradually but only to a limited extent, although the crystallinity at 200 °C was significant. Recrystallisation resulted in decreased transparency, increased reflectivity, and a red shift to lower band gap. The methylene blue (MB) degradation was in the order 25 °C < 200 °C < 100 °C < 150 °C, indicating that the photocatalytic performance was dominated by crystallinity and exposed surface area, the latter of which decreased at 200 °C owing to partial submergence in the softened polymeric substrate. The films indicated antimicrobial capacity since they exhibited a maximal level of S. aureus destruction (85%) following 5 h of UV irradiation. The films also appeared to be microstructurally stable following wash testing in contact lens washing solution.

Simulation study of anisotropic flow resistance of farmland vegetation

Farmland vegetation is commonly cultivated with uniform planting spacing and heights. The effect of these features on resistance to hydraulic erosion is unclear. Hydraulic model experiments with the angle between the crop rows and the water flow direction set at 15°, 30°, 45° or 90° were conducted to analyze variation in the law of water flow resistance under partial or complete submergence of the crop. Cultivation can impact the flow resistance on slopes and this effect was greater when the crop was partially submerged. When planting spacing, slope, and water depth were constant, the change of the water flow Darcy-Weisbach resistance coefficient f with crop row-water flow angle was f&lt;sub&gt;15&lt;/sub&gt;° &amp;gt; f&lt;sub&gt;30&lt;/sub&gt;° &amp;gt; f&lt;sub&gt;45&lt;/sub&gt;° &amp;gt; f&lt;sub&gt;90&lt;/sub&gt;°. This suggests that flow resistance of farmland vegetation is anisotropic. The water flow resistance coefficient of crops that were partly submerged increased with water depth, but decreased with water depth when the crop was completely submerged. At the critical change from partial submergence to complete submergence, the water flow resistance coefficient was the highest when water depth was equal to crop height. These results may be useful for optimizing farmland planting and soil and water conservation.

Numerical and experimental investigation on surface air entrainment mechanisms of a novel long‐short blades agitator

We investigate numerically and experimentally the mechanisms of surface air entrainment in the vessels equipped with the long‐short blades agitator. VOF method coupled with LES model is used to visualize the surface air entrainment process. In the case of partial submergence of the long blades (LBs), the interaction of the LBs with the liquid free surface creates a depression behind the LBs. Backfilling of the liquid into the depression leads to gas separation and entrapping into the liquid. The critical tip velocity of the LBs, utip,c, for the onset of gas entrainment is measured in vessels with diameters, T = 200∼600 mm. It is found that when H/T ≥ 1.0, utip,c is determined by the LBs, independent of the liquid level. utip,c is also affected by the size of the vessel through the diameter of the sweeping circle of the LBs, but for substantially large vessels, it approaches a constant value. © 2017 American Institute of Chemical Engineers AIChE J, 63: 316–325, 2018

Fitness and growth of the ephemeral mudflat species Cyperus fuscus in river and anthropogenic habitats in response to fluctuating water-levels

Cyperus fuscus is a representative of threatened ephemeral wetland plant communities in summer-dry shoreline habitats. We compared variation and plasticity in traits related to fitness and growth of plants germinating from the soil seed bank and established plants from river and secondary anthropogenic habitats. Plants from sites at rivers, fishponds and fish storage ponds were cultivated and selfed to get homogenous seed material for a germination and an environmental manipulation experiment involving three different water regimes. Differences in traits and their plasticities were evaluated by means of linear mixed models. Cyperus fuscus followed a low-oxygen escape strategy when flooded. Seeds of plants derived from the soil seed bank germinated faster than seeds of plants derived from established plants suggesting that short-term selection of genotypes is mediated by the particular conditions on the site during germination. The experiment revealed significant differences between river and secondary habitats as well as between the soil seed bank and established plants. For example, plants from river habitats produced the highest number of culms with inflorescences. The difference was most evident under partial submergence. Plants from fish storage ponds rapidly reached the reproductive phase, but produced less culms with inflorescences. This seemingly allows them to cope with numerous and irregular disturbances and intensive substrate moisture changes. Our results suggest that populations have adapted to conditions at secondary habitats provided by fish farming during the last centuries.

Benefits of flooding-induced aquatic adventitious roots depend on the duration of submergence: linking plant performance to root functioning.

Temporal flooding is a common environmental stress for terrestrial plants. Aquatic adventitious roots (aquatic roots) are commonly formed in flooding-tolerant plant species and are generally assumed to be beneficial for plant growth by supporting water and nutrient uptake during partial flooding. However, the actual contribution of these roots to plant performance under flooding has hardly been quantified. As the investment into aquatic root development in terms of carbohydrates may be costly, these costs may - depending on the specific environmental conditions - offset the beneficial effects of aquatic roots. This study tested the hypothesis that the balance between potential costs and benefits depends on the duration of flooding, as the benefits are expected to outweigh the costs in long-term but not in short-term flooding. The contribution of aquatic roots to plant performance was tested in Solanum dulcamara during 1-4 weeks of partial submergence and by experimentally manipulating root production. Nutrient uptake by aquatic roots, transpiration and photosynthesis were measured in plants differing in aquatic root development to assess the specific function of these roots. As predicted, flooded plants benefited from the presence of aquatic roots. The results showed that this was probably due to the contribution of roots to resource uptake. However, these beneficial effects were only present in long-term but not in short-term flooding. This relationship could be explained by the correlation between nutrient uptake and the flooding duration-dependent size of the aquatic root system. The results indicate that aquatic root formation is likely to be selected for in habitats characterized by long-term flooding. This study also revealed only limited costs associated with adventitious root formation, which may explain the maintenance of the ability to produce aquatic roots in habitats characterized by very rare or short flooding events.

Physiological Basis of Stagnant Flooding Tolerance in Rice

Stagnant flooding (SF) is an important constraint which prolonges partial submergence damages of rice plants and reduces grain yield. Due to the heterogeneity in flood-prone ecosystem, many different types of traditional rice varieties are being grown by the farmers. The local landraces adapted to extreme in water availability could be the sources of new gene(s) which would be utilized to improve the adaptability of rice to SF with high yield. The main goal of this study is to identify new genetic resources tolerant to SF based on morpho-physiological traits. A total of 16 rice varieties were selected after initial screening from more than 400 rice varieties which were collected from eastern states of India. The increase rate of plant height was higher under SF compared to control, whereas no such trend was observed in the increment rate of aboveground total dry weight and culm dry weight. Area of aerenchyma gas spaces per tiller increased whereas root oxidase activity decreased under SF. The reduction of root oxidase activity, leaf area, and leaf dry weight was higher in susceptible varieties under SF compared to control. Stability index for different grain yield and yield attributes revealed that the impact of SF differed among different varieties. Correlation coefficient studies among different parameters taking stability index showed significant association with the grain yield. Based on the findings, it was concluded that maintenance of equivalent panicle weight and panicle number, plant height and harvest index at the maturity stage, leaf area, leaf and culm dry weights, root oxidase activity and tiller numbers at the flowering stage under SF compared to control help the plant to counteract the adverse effects of SF.

Contrasting oxygen dynamics in Limonium narbonense and Sarcocornia fruticosa during partial and complete submergence.

Terrestrial saltmarsh plants inhabiting flood-prone habitats undergo recurrent and prolonged flooding driven by tidal regimes. In this study, the role of internal plant aeration in contrasting hypoxic/anoxic conditions during submergence was investigated in the two halophytes Limonium narbonense Mill. and Sarcocornia fruticosa (L.) A.J. Scott. Monitoring of tissue O2 dynamics was performed in shoots and roots using microelectrodes under drained conditions, waterlogging, partial and complete submergence, in light or darkness. For both species, submergence in darkness resulted in significant declines in tissue O2 status and when in light, in rapid O2 increases first in shoot tissues and subsequently in roots. During partial submergence, S. fruticosa benefitted from snorkelling and efficiently transported O2 to roots, whereas the O2 concentration in roots of L. narbonense declined by more than 90%. Significantly thinner leaves and articles were recorded under high degree of flooding stress and both species showed considerably high tissue porosity. The presence of aerenchyma seemed to support internal aeration in S. fruticosa whereas O2 diffusion in L. narbonense seemed impeded, despite the higher porosity (up to 50%). Thus, the results obtained for L. narbonense, being well adapted to flooding, suggests that processes other than internal aeration could be involved in better flooding tolerance e.g. fermentative processes, and that traits resulting in flooding tolerance in plants are not yet fully understood.

Environmental factors constraining adventitious root formation during flooding of Solanum dulcamara.

Flooding is a compound stress, imposing strong limitations on plant development. The expression of adaptive traits that alleviate flooding stress may be constrained if floodwater levels are too deep. For instance, adventitious root outgrowth is typically less profound in completely submerged plants than in partially submerged plants, suggesting additional constraints in full submergence. As both oxygen and carbohydrates are typically limited resources under submergence, we tested the effects of oxygen concentration in the floodwater and carbohydrate status of the plants on flooding-induced adventitious root formation in Solanum dulcamara L. Partially submerged plants continued to form adventitious roots in low-oxygen floodwater, whereas completely submerged plants developed hardly any roots, even in floodwater with twice the ambient oxygen concentration. This suggests that contact with the atmosphere, enabling internal aeration, is much more important to optimal adventitious root formation than floodwater oxygen concentrations. If plants were depleted of carbohydrates before flooding, adventitious root formation in partial submergence was poor, unless high light was provided. Thus, either stored or newly produced carbohydrates can fuel adventitious root formation. These results imply that the impact of an environmental stress factor like flooding on plant performance may strongly depend on the interplay with other environmental factors.

Physiological Responses and Expression Profile of NADPH Oxidase in Rice (Oryza Sativa) Seedlings under Different Levels of Submergence.

BackgroundFlooding due to global climate change is a serious problem that frequently decreases crop yields. Rice fields in flood-prone areas often experience full or partial submergence. Submergence has an adverse effect on internal oxygen availability, sugar status and survival. Complete submergence imposes severe pressure on plants, principally because the excess water in their surroundings deprives them of certain basic resources such as oxygen, carbon dioxide and light for photosynthesis. To better understand the mechanisms involved under different levels of flooding, it is necessary to further observe physiological responses and to identify the Rboh genes involved and determine how they are regulated during submergence.ResultsIn this study, significant physiological changes were observed in plant height, leaf sheath elongation and chlorophyll a, b and total content under partial and full submergence treatments. Senescence-regulating genes were severely affected under full submergence. Additionally, intracellular oxidative homeostasis was disrupted by overproduction of H2O2 and O2−, which affected cell viability and antioxidant enzyme activity, under different levels of submergence. Quantitative RT-PCR analyses revealed that complex regulation of Rboh genes is involved under different levels of submergence.ConclusionOur results demonstrated that the effect of physiological and the transcript levels of OsRboh genes were presented different responses to different levels of submergence in rice seedlings. There have different mechanism in intracellular to response different levels of submergence. Finally we discuss effects of the regulation of OsRboh expression and ROS production which was important to maintain homeostasis to help rice seedlings face different levels of submergence.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-016-0074-9) contains supplementary material, which is available to authorized users.

Ethylene is not involved in adaptive responses to flooding in the Amazonian wild rice species Oryza grandiglumis

The Amazonian wild rice Oryza grandiglumis has two contrasting adaptation mechanisms to flooding submergence: a quiescence response to complete submergence at the seedling stage and an escape response based on internodal elongation to partial submergence at the mature stage. We investigated possible factors that trigger these responses. In stem segments excised from mature O. grandiglumis plants, complete submergence only slightly promoted internodal elongation with increased ethylene levels in the internodes, while partial submergence substantially promoted internodal elongation without increased ethylene levels in the internodes. Incubation of non-submerged stem segments under a continuous flow of humidified ethylene-free air promoted internodal elongation to the same extent as that observed for partially submerged segments. Applied ethylene had little effect on the internodal elongation of non-submerged segments irrespective of humidity conditions. These results indicate that the enhanced internodal elongation of submerged O. grandiglumis plants is not triggered by ethylene accumulated during submergence but by the moist surroundings provided by submergence. The growth of shoots in O. grandiglumis seedlings was not promoted by ethylene or complete submergence, as is the case in O. sativa cultivars possessing the submergence-tolerant gene SUB1A. However, because the genome of O. grandiglumis lacks the SUB1A gene, the quiescence response of O. grandiglumis seedlings to complete submergence may be regulated by a mechanism distinct from that involved in the response of submergence-tolerant O. sativa cultivars.

Physiological analyses of traits associated with tolerance of long-term partial submergence in rice.

Floods are major constraints to crop production worldwide. In low-lying, flood-prone areas of the tropics, longer-term partial submergence (stagnant flooding [SF]) greatly reduces rice yield. This study assesses shoot growth and several physiological mechanisms associated with SF tolerance in rice. Five rice genotypes with contrasting responses to SF were evaluated in field ponds. Following transplanting, floodwater was gradually increased at a rate of ∼2 cm day(-1) to reach a final depth of 50 cm and then maintained until maturity. Although plants were not fully submerged, the yield was reduced by 47 % across genotypes compared with those grown under control conditions (6.1 vs. 3.3 t ha(-1)). This reduction was mainly attributed to the reduction in biomass caused by reduced light interception and leaf growth above the water. Stagnant flooding also reduced panicle number per unit area by 52 % because of reduced tillering. Shoot elongation rate kept pace with rising floodwater and correlated positively with leaf growth and biomass production. Conversely, stem non-structural carbohydrate (NSC) concentration correlated negatively with shoot elongation rate, suggesting that fast-elongating genotypes actively consume NSCs to avoid complete submergence. Moderate shoot elongation rate strongly and positively correlated with grain yield under SF; however, elongation at rates >2.0 cm day(-1) was associated with reduced harvest index due to a smaller panicle size and increased lodging. Tolerant varieties were found to be either inherently tall or elongate moderately with rising floodwater. Our studies suggest that to improve tolerance of SF an appropriate phenotype should combine both of these traits. Fine-tuning for optimum shoot elongation with rising floodwater is, therefore, a priority for future work.

Environmental water allocations are insufficient to control an invasive wetland plant: evidence from a highly regulated floodplain wetland

Summary Regulation of the world's rivers and alteration of natural flow regimes have led to the degradation of floodplains and wetlands. Here, we investigate plant–flood interactions in a floodplain wetland to determine whether a return to more natural flooding regimes using environmental water allocations can contribute to restoration from a degraded state. We use detailed field surveys to quantify the effects of flooding on a native rush, Juncus ingens, which has become invasive in floodplain grasslands in south‐eastern Australia. We then apply these results to determine the likelihood of generating the flood regime required to control J. ingens in its new range, considering the regulated nature of the river system. Complete submergence for several months substantially reduced J. ingens stem densities, survival and recovery. However, rhizomes were able to survive, with new stems produced during subsequent partial submergence. Under current regulated conditions, the volume of environmental water needed to cause substantial stem decline, and potentially rhizome death, is extremely large – particularly for tall stems – even when used in conjunction with a natural flood peak. Synthesis and applications. Although repeated submergence events may cause stem decline of the invasive plant Juncus ingens, the present day operational constraints of the regulated river make it difficult to deliver the required volume and depth of water using environmental water allocations. Furthermore, shallow floods may actually provide favourable conditions for growth. This research is particularly relevant for other restoration projects where historical disturbance regimes can only be partially mimicked. Whilst a managed flood regime can be successful in achieving a range of conservation and restoration goals, there may be situations in which river regulation can limit the ability to effectively manage particular species using environmental water allocations alone.

The role of nodules in the tolerance of common bean to iron deficiency

Iron is vital for the establishment and function of symbiotic root nodules of legumes. Although abundant in the environment, Fe is often a limiting nutrient for plant growth due to its low solubility and availability in some soils. We have studied the mechanism of iron uptake in the root nodules of common bean to evaluate the role of nodules in physiological responses to iron deficiency. Based on experiments using full or partial submergence of nodulated roots in the nutrient solution, our results show that the nodules were affected only slightly under iron deficiency, especially when the nodules were submerged in nutrient solution in the tolerant cultivar. In addition, fully submerged root nodules showed enhanced acidification of the nutrient solution and showed higher ferric chelate reductase activity than that of partially submerged roots in plants cultivated under Fe deficiency. The main results obtained in this work suggest that in addition to preferential Fe allocation from the root system to the nodules, this symbiotic organ probably develops some mechanisms to respond to iron deficiency. These mechanisms were implied especially in nodule Fe absorption efficiency and in the ability of this organ to take up Fe directly from the medium.

Metabolic adaptation to sugar/O2 deficiency for anaerobic germination and seedling growth in rice

Rice is characterized by a broad range of metabolic and morphological adaptations to flooding, such as germination and mobilization of stored nutrients under submergence until seedlings reach the water surface to carry out photosynthesis, and sustainable growth of mature plants for long durations under partial submergence. The underlying mechanisms of the molecular basis of adaptation to anaerobic germination and seedling growth in rice are being uncovered. Induction of an ensemble of hydrolases to mobilize endosperm nutrient reserves is one of the key factors for successful germination and coleoptile elongation in rice under submergence. To compensate for reduced efficiency of Tricarboxylic Acid cycle and oxidative respiration in mitochondria under O2 deficient conditions, α-amylases play a central role in the hydrolysis of starch to provide sugar substrates for glycolysis and alcohol fermentation for generating ATP. We review the progress on the molecular mechanism regulating α-amylase expression that involves the integration of signals generated by the hormone gibberellin (GA), sugar starvation and O2 deprivation that results in germination and sustainable seedling growth in rice under anaerobic conditions. Comparisons are also made between dicots and monocots for the molecular mechanism of induction of genes involved in alcohol fermentation and sugar/O2 deficiency sensing system.

Partial versus complete submergence: snorkelling aids root aeration in Rumex palustris but not in R. acetosa

The root and shoot tissues of flood-tolerant wetland plants are highly porous to enable internal gas phase diffusion of O2 during waterlogging or submergence. In the case of only partial submergence (snorkelling), the atmosphere can act as source of O2 . The aim of this study was to assess the effect of waterlogging, partial submergence and complete submergence in the dark as well as in light on O2 partial pressure (pO2 ) in roots of Rumex palustris (flood tolerant) and R. acetosa (flood intolerant). We used O2 microelectrodes to measure pO2 of adventitious roots during manipulations of the water level around the shoot. Root pO2 in both species declined significantly upon submergence but remained oxic also when shoots were completely submerged in the dark (0.8 and 4.6 kPa in R. acetosa and R. palustris, respectively). The snorkelling effect was substantial in R. palustris only. Submergence in light had less impact on root pO2 and the effect of snorkelling was also minor. Hence, the benefits of light (underwater photosynthesis) and air contact (snorkelling) upon growth and survival in submerged wetland plants can now be linked to enhanced internal aeration.

Rapid flooding-induced adventitious root development from preformed primordia in Solanum dulcamara.

Flooding is a common stress factor in both natural and agricultural systems, and affects plant growth by the slow diffusion rate of gases in water. This results in low oxygen concentrations in submerged tissues, and hence in a decreased respiration rate. Understanding the responses of plants to flooding is essential for the management of wetland ecosystems, and may benefit research to improve the flood tolerance of crop species. This study describes the response to partial submergence of bittersweet (Solanum dulcamara). Bittersweet is a Eurasian species that grows both in dry habitats such as coastal dunes, and in wetlands, and therefore is a suitable model plant for studying responses to a variety of environmental stresses. A further advantage is that the species is closely related to flood-intolerant crops such as tomato and eggplant. The species constitutively develops dormant primordia on the stem, which we show to have a predetermined root identity. We investigated adventitious root growth from these primordia during flooding. The synchronized growth of roots from the primordia was detected after 2-3 days of flooding and was due to a combination of cell division and cell elongation. Gene expression analysis demonstrated that the molecular response to flooding began within 2 h and included activation of hypoxia and ethylene signalling genes. Unexpectedly, these early changes in gene expression were very similar in primordia and adjacent stem tissue, suggesting that there is a dominant general response in tissues during early flooding.

Numerical and experimental study of a nearby solid boundary and partial submergence effects on hydrofoil added mass

The influence of the boundary conditions on the added mass of a NACA0009 cantilever hydrofoil has been numerically and experimentally investigated. The study has been focused on the effects of a lateral solid wall close to the hydrofoil tip side and of a partially wetted vertical hydrofoil at different levels with the free surface parallel to the span. A detailed fluid–structure model has been built for both cases and a modal analysis has been carried out. The numerical three first natural frequencies have been used to calculate the added mass coefficients. These coefficients have been compared with the results of the analogous experiments carried out by means of an onboard excitation and response system based on piezoelectric patches. The obtained results are in reasonable good agreement with experimental data. The proximity of a lateral solid wall provokes an increase of the added mass experienced by the hydrofoil which can be approximated by a 2nd order polynomial function for both bending and torsion modes. On the other hand, the increase of the added mass with the level of partial submergence is found to be dependent on the relative displacements of the hydrofoil submerged part and hence on the mode shape.