Partial Nitritation-anammox Research Articles

Segregation of effect between granules and flocs in PN/A system treating acrylic fiber wastewater: Performance and mechanism.

Nitrogen removal of petrochemical wastewater through partial nitritation/anammox (PN/A) is appealing, but its feasibility and stability under toxic inhibition remain unclear. This study started a PN/A granular sludge system in a membrane bioreactor and fed it with diluted acrylic fiber wastewater. During long-term operation, the nitritation and anammox performance remained stable at a 30% volume ratio, and declined with increasing volume ratio, resulting in deteriorated nitrogen removal. Meanwhile, the short-term inhibition batch tests further showed that ammonia oxidation bacteria (AOB) in the flocs were suppressed while anammox bacteria (AnAOB) in the granules were not affected. Further analysis indicated suppression of AnAOB over the long-term operation, which was mainly caused by the disintegration of granules as demonstrated by sludge morphology. This selective inhibition is associated with variational sludge morphology, and the distribution of functional bacteria plays an important role in the feasibility and stability of PN/A treating acrylic fiber wastewater. As above, this study demonstrated the feasibility of PN/A for acrylic fiber wastewater treatment, but wastewater dilution or pre-treatment is still required for efficient nitrogen removal.

A quantified nitrogen metabolic network by reaction kinetics and mathematical model in a single-stage microaerobic system treating low COD/TN wastewater

A single-stage intermittent aeration microaerobic reactor (IAMR) has been developed for the cost-effective nitrogen removal from piggery wastewater with a low ratio of chemical oxygen demand (COD) to total nitrogen (TN). In this study, a quantified nitrogen metabolic network was constructed based on the metagenomics, reaction kinetics and mathematical model to provide a revealing insight into the nitrogen removal mechanism in the IAMR. Metagenomics revealed that a complex nitrogen metabolic network, including aerobic ammonia and nitrite oxidation, anammox, denitrification via nitrate and nitrite, and nitrate respiration, existed in the IAMR. A novel method for solving kinetic parameters with high stability was developed based on a genetic algorithm. Use this method to calculate the kinetics of various reactions involved in nitrogen metabolism. Kinetics revealed that simultaneous partial nitritation-anammox (PN/A) and partial denitrification-anammox (PDN/A) were the dominant approaches to nitrogen removal in the IAMR. Finally, a kinetics-based model was proposed for quantitatively describing the nitrogen metabolic network under the limitation of COD. 58% ∼ 67% of nitrogen was removed via the anammox-based processes (PN/A and PDN/A), but only 7% ∼ 12% and 1% ∼ 2% of nitrogen were removed via heterotrophic denitrification of nitrite and nitrate, respectively. The half-inhibition constant of dissolved oxygen (DO) on anammox was simulated as 0.37 ∼ 0.60 mg L−1, filling the gap in quantifying DO inhibition on anammox. High-frequency intermittent aeration was identified as the crucial measure to suppress nitrite-oxidizing bacteria, although it has a high affinity for DO and NO2−-N. In continuous aeration mode, the simulated NO3−-N in the IAMR would rise by 39.6%. The research provides a novel insight into the nitrogen removal mechanism in single-stage microaerobic systems and provides a reliable approach to practicing PN/A and PDN/A for cost-effective nitrogen removal.

Advanced nitrogen removal from municipal sewage via partial nitrification-anammox process under two typical operation modes and seasonal ambient temperatures

A novel two-stage partial nitrification-anammox (PN-A) process was developed, achieving nitrogen removal from low carbon/nitrogen ratio municipal sewage under two typical operational modes and seasonal ambient temperatures. When complete nitritation-anammox was performed at temperatures greater than 19.4 °C, the effluent concentration of total inorganic nitrogen (TIN) was 4.1 mg/L, corresponding to a nitrogen removal efficiency (NRE) of 94.3 %. In contrast, when partial nitritation-anammox was performed at temperatures below 19.4 °C, the effluent TIN was 12.3 mg/L, corresponding to a NRE of 83.6 %. The relative abundance of Nitrosomonas and Nitrosomonadaceae increased from 0.02 % to 0.28 %, while Ca. Brocadia decreased from 1.85 % to 1.30 %, with the contribution of anammox to nitrogen removal being highest under low temperatures (19.4℃ to 13.8℃), at 59.0 %. This novel two-stage PN-A process provides a new approach for the stable operation of wastewater treatment plants (WWTPs) under low ambient temperatures.

Insights into rapidly recovering the autotrophic nitrogen removal performance of single-stage partial nitritation-anammox systems: Reconstructing granular sludge and its functional microbes synergy

Partial nitritation-anammox (PNA) deteriorates easily and is difficult to recover. After an airlift inner-circulation partition bioreactor was impacted by low NH4+–N wastewater containing organic matter, Nitrospira and Denitratisoma propagated rapidly, granular sludge disintegrated, and the total nitrogen removal efficiency (TNRE) decreased from 68.27 % to 5.97 %. This study used a unique strategy to recover deteriorated single-stage PNA systems and explored the mechanism of rapid performance recovery. The TNRE of the system recovered up to 61.77 % in 43 days. The high nitrogen loading rate and hydraulic shear force from the airlift caused the sludge in the reactor to granulate again. The microbial community structure recovered, with a decrease in the abundance of Nitrospira (0.05 %) and enrichment of Candidatus Brocadia (8.82 %). A favorable synergy among functional microbes in the reactor was thus re-established, promoting the rapid recovery of the nitrogen removal performance. This study provides a feasible recovery strategy for PNA processes.

Efficient management of the nitritation-anammox microbiome through intermittent aeration: absence of the NOB guild and expansion and diversity of the NOx reducing guild suggests a highly reticulated nitrogen cycle

Obtaining efficient autotrophic ammonia removal (aka partial nitritation-anammox, or PNA) requires a balanced microbiome with abundant aerobic and anaerobic ammonia oxidizing bacteria and scarce nitrite oxidizing bacteria. Here, we analyzed the microbiome of an efficient PNA process that was obtained by sequential feeding and periodic aeration. The genomes of the dominant community members were inferred from metagenomes obtained over a 6 month period. Three Brocadia spp. genomes and three Nitrosomonas spp. genomes dominated the autotrophic community; no NOB genomes were retrieved. Two of the Brocadia spp. genomes lacked the genomic potential for nitrite reduction. A diverse set of heterotrophic genomes was retrieved, each with genomic potential for only a fraction of the denitrification pathway. A mutual dependency in amino acid and vitamin synthesis was noted between autotrophic and heterotrophic community members. Our analysis suggests a highly-reticulated nitrogen cycle in the examined PNA microbiome with nitric oxide exchange between the heterotrophs and the anammox guild.

Insights into the carbon neutrality for the treatment process engineering of municipal wastewater by anaerobic membrane bioreactor integrated with partial nitritation-anammox: CO2 reduction and energy recovery

The promising sustainability and potential in carbon neutrality of the anaerobic membrane bioreactor (AnMBR) integrated with partial nitritation-anammox (PN/A) was assessed using a large pilot-scale plant. The entire pilot-scale system was conducted for a long-term continuous operation of 217 days, succeeding in the effective carbon-neutral treatment of low-strength real municipal wastewater with excellent COD removal efficiency of higher than 90 %. The major proportion of the organic matter was converted into bio-methane which can be recovered as bio-energy or bio-fuel. The residual ammonium contained in the digestate effluent of the AnMBR was further treated by a pilot-scale one-stage PN/A reactor. The long-term operation of the pilot-scale AnMBR was compared with the empirical assumption of the anaerobic-anoxic-oxic (A2/O) process, exhibiting splendid feasibilities in both ecological and economical perspectives including energy conservation and recovery, reduction of CO2 emission and electricity expenditure saving for municipal wastewater treatment. The application of AnMBR-PN/A process helps reducing the energy consumption of 0.416 kWh/m3 during the wastewater treatment process engineering of municipal wastewater, accounting for 90 % of that for the A2/O. The AnMBR-PN/A is also capable of reducing the CO2 emission reduced by 74.9 %. A full-scale wastewater treatment plant implemented with the AnMBR-PN/A process could save the electricity expenditure by 0.80 million CNY annually. The results of this work are solid evidence that the AnMBR-PN/A process is a remarkable sustainable technology, also giving new insights into the concept of carbon-neutral treatment of municipal wastewater in future studies.

Enhanced nitrogen removal of single stage partial nitritation anammox system by glycine betaine addition at low temperature: Performance and mechanism

Low activity of anaerobic ammonia oxidizing bacteria (AnAOB) at low temperature is still an obstacle to the application of a single stage partial nitritation anammox (SPNA) process. In order to improve the performance of nitrogen removal of this system at low ambient temperature, the glycine betaine (GB) was added into a continuous stirred tank reactor (CSTR) at 13–15 °C. At 15 °C, the nitrogen removal rate (NRR) was increased from 0.48 to 0.62 g N/(L·d) by adding 1 mM GB with the nitrogen loading rate of 0.81 g N/(L·d). The activity of hydrazine oxidase (HZO) was enhanced from 15.78 to 19.81 μmol Cyt-c/(g VSS·min), while the extracellular polymeric substances (EPS) production was reduced from 290.31 to 109.06 mg/g VSS after exogenous GB. When the temperature was <15 °C, however, the promoting effect of 1 mM GB was weakened, and the NRR was 0.43 g N/(L·d) at 13 °C. Increasing the addition of GB to 1.5 mM did not improve nitrogen removal performance at 13 °C, whereas it promoted the enrichment of heterotrophic bacteria (HB). Overall, results suggested GB had the potential to enhance the metabolic capability of AnAOB, which provided a novel strategy for improving the nitrogen removal performance of SPNA system at low temperature.

Successful year-round mainstream partial nitritation anammox: Assessment of effluent quality, performance and N2O emissions

For two decades now, partial nitritation anammox (PNA) systems were suggested to more efficiently remove nitrogen (N) from mainstream municipal wastewater. Yet to date, only a few pilot-scale systems and even fewer full-scale implementations of this technology have been described. Process instability continues to restrict the broad application of PNA. Especially problematic are insufficient anammox biomass retention, the growth of undesired aerobic nitrite-oxidizers, and nitrous oxide (N2O) emissions. In this study, a two-stage mainstream pilot-scale PNA system, consisting of three reactors (carbon pre-treatment, nitritation, anammox - 8 m3 each), was operated over a year, treating municipal wastewater. The aim was to test whether both, robust autotrophic N removal and high effluent quality, can be achieved throughout the year. A second aim was to better understand rate limiting processes, potentially affecting the overall performance of PNA systems. In this pilot study, excellent effluent quality, in terms of inorganic nitrogen, was accomplished (average effluent concentrations: 0.4 mgNH4-N/L, 0.1 mgNO2-N/L, 0.9 mgNO3-N/L) even at wastewater temperatures previously considered problematic (as low as 8 °C). N removal was limited by nitritation rates (84 ± 43 mgNH4-N/L/d), while surplus anammox activity was observed at all times (178 ± 43 mgN/L/d). Throughout the study, nitrite-oxidation was maintained at a low level (<2.5% of ammonium consumption rate). Unfortunately, high N2O emissions from the nitritation stage (1.2% of total nitrogen in the influent) were observed, and, based on natural isotope abundance measurements, could be attributed to heterotrophic denitrification. In situ batch experiments were conducted to identify the role of dissolved oxygen (DO) and organic substrate availability in N2O emission-mitigation. The addition of organic substrate, to promote complete denitrification, was not successful in decreasing N2O emission, but increasing the DO from 0.3 to 2.9 mgO2/L decreased N2O emissions by a factor of 3.4.

Application of two-stage nitritation/anammox using activated sludge immobilized on polyvinyl alcohol/alginate with addition of foaming agent: Pilot-scale study

The non-porous structure of the outer layer in the existing polyvinyl alcohol (PVA)/alginate carrier lowered the stability and efficiency of the partial nitritation/anammox (PN/A) process. In this study, these drawbacks could be improved with the addition of a foaming agent, and the effect was evaluated in a two-step PN/A process on a pilot scale. The penetration efficiency for dissolved oxygen was a parameter for determining the optimal content of the foaming agent, and it could be determined as 0.3% w/v. In the pilot PN, the DO diffusion inside the PVA/alginate carrier was effective for selective nitrite oxidizing bacteria (NOB) inhibition. As a result, ammonium oxidation efficiency of 49.3 ± 2.7% was achieved within 20 d of operation, resulting in a NO2−-N/NH4+-N ratio of 0.92 ± 0.1 in the effluent. In the pilot anammox, the contribution of endogenous denitrification to nitrogen removal (95.6 ± 2.9%) was a major factor inhibiting the physiological activity of anammox. The strategy of decreasing the hydraulic retention time worked effectively in alleviating the negative impact, which improved the contribution of anammox to nitrogen removal. In addition, this strategy was also worked effective in alleviating the effect of inhibitors on anammox activity under field conditions. With stabilization of nitrogen removal by anammox, exogenous denitrification even contributed to improving nitrogen removal efficiency, and a nitrogen removal rate of 1.1 ± 0.1 kg/m3/d was achieved at an influent nitrogen load of 1.4 ± 0.1 kg/m3/d.

Disturbance-based management of ecosystem services and disservices in partial nitritation-anammox biofilms.

The resistance and resilience provided by functional redundancy, a common feature of microbial communities, is not always advantageous. An example is nitrite oxidation in partial nitritation-anammox (PNA) reactors designed for nitrogen removal in wastewater treatment, where suppression of nitrite oxidizers like Nitrospira is sought. In these ecosystems, biofilms provide microhabitats with oxygen gradients, allowing the coexistence of aerobic and anaerobic bacteria. We designed a disturbance experiment where PNA biofilms, treating water from a high-rate activated sludge process, were constantly or intermittently exposed to anaerobic sidestream wastewater, which has been proposed to inhibit nitrite oxidizers. With increasing sidestream exposure we observed decreased abundance, alpha-diversity, functional versatility, and hence functional redundancy, among Nitrospira in the PNA biofilms, while the opposite patterns were observed for anammox bacteria within Brocadia. At the same time, species turnover was observed for aerobic ammonia-oxidizing Nitrosomonas populations. The different exposure regimens were associated with metagenomic assembled genomes of Nitrosomonas, Nitrospira, and Brocadia, encoding genes related to N-cycling, substrate usage, and osmotic stress response, possibly explaining the three different patterns by niche differentiation. These findings imply that disturbances can be used to manage the functional redundancy of biofilm microbiomes in a desirable direction, which should be considered when designing operational strategies for wastewater treatment.

Morphological and Spatial Heterogeneity of Microbial Communities in Pilot-Scale Autotrophic Integrated Fixed-Film Activated Sludge System Treating Coal to Ethylene Glycol Wastewater.

The understanding of microbial compositions in different dimensions is essential to achieve the successful design and operation of the partial nitritation/anammox (PN/A) process. This study investigated the microbial communities of different sludge morphologies and spatial distribution in the one-stage PN/A process of treating real coal to ethylene glycol (CtEG) wastewater at a pilot-scale integrated fixed-film activated sludge (IFAS) reactor. The results showed that ammonia-oxidizing bacteria (AOB) was mainly distributed in flocs (13.56 ± 3.16%), whereas anammox bacteria (AnAOB) was dominated in the biofilms (17.88 ± 8.05%). Furthermore, the dominant AnAOB genus in biofilms among the first three chambers was Candidatus Brocadia (6.46 ± 2.14% to 11.82 ± 6.33%), whereas it was unexpectedly transformed to Candidatus Kuenenia (9.47 ± 1.70%) and Candidatus Anammoxoglobus (8.56 ± 4.69%) in the last chamber. This demonstrated that the niche differentiation resulting from morphological (dissolved oxygen) and spatial heterogeneity (gradient distribution of nutrients and toxins) was the main reason for dominant bacterial distribution. Overall, this study presents more comprehensive information on the heterogeneous distribution and transformation of communities in PN/A processes, providing a theoretical basis for targeted culture and selection of microbial communities in practical engineering.

Revealing the effect of biofilm formation in partial nitritation-anammox systems: Start-up, performance stability, and recovery

Successful application of partial nitritation-anammox (PNA) processes is currently and primarily associated with biofilm systems. Biofilm characteristics significantly influence start-up, performance stability, and recovery. Here, two PNA systems with and without carriers were implemented simultaneously for treating wastewater containing 50 mg-NH4/L. The performance characteristics of these two PNA systems were compared. Stable nitrogen removal efficiencies of 76.3 ± 2.8% and 72.9 ± 1.6% were obtained for suspended sludge and biofilm systems, respectively. The slow process of biofilm colonization resulted in a long start-up time in the biofilm system. Biofilm enrichment and protection conferred stable performance when exposed to aeration shock. The suspended sludge system displayed good elasticity during performance recovery after shock compared to the slow recovery in the biofilm system. Moreover, suitable control of dissolved oxygen could improve the activity and abundance of the functional microbes. This study provides new insights into the operation and control of PNA systems for treating mainstream wastewater.

One-stage partial nitritation-anammox treatment of reject water from high-solid-sludge anaerobic digestion with thermal hydrolysis pretreatment: Inhibition and system recovery

This study investigated the performance of a one-stage partial nitritation-anammox (PN/A) system for the treatment of reject water from high-solid-sludge anaerobic digestion with thermal hydrolysis pretreatment (THP-AD). The proportion of reject water was gradually increased (10.8%, 23.2%, 36.4%, 73.7% and 100%) during the 90-day operation. The nitrogen loading rate (NLR) reached 0.44 kg N/m3/d using 73.7% reject water as influent, while it decreased to only 0.15 kg N/m3/d with 100% reject water. Meanwhile, sludge particles with diameters < 180 µm became the largest granulometric fraction. Microbial community structure analysis indicated that the relative abundances of functional microorganisms became extremely low; e.g., 0.05% and 0.5% for Nitrosomonas and Candidatus Brocadia, respectively. An additional 47-day experiment was performed using only the anammox stage to evaluate the inhibition impacts of organics. This was fed with reject water with partial ammonia nitrogen stripping and nitrite nitrogen addition for the first 38 days and PN-treated reject water for days 39–47. Reject water with an 80% raw organic content demonstrated severe inhibition, with the NLR dropping from 1.27 kg N/m3/d to 0.27 kg N/m3/d. This, could be partially restored to 0.93 kg N/m3/d when the influent was pretreated by PN. It appears that the biodegradable organic matter inhibited the one-stage PN/A system more than the non-biodegradable fraction. After removal of biodegradable organics, the NRR of the anammox system recovered, indicating that two-stage PN/A has greater advantages in THP-AD reject water treatment than a one-stage PN/A system.

Metagenomes from 25 Low-Abundance Microbes in a Partial Nitritation Anammox Microbiome.

ABSTRACTMicrobial communities using anammox bacteria to remove nitrogen are increasingly important in wastewater treatment. We report on 25 metagenome-assembled genomes of low-abundance microbes from a partial nitritation anammox bioreactor system that have not been described previously. These data add to the body of information about this important wastewater treatment system.

Integrated thermal hydrolysis pretreated anaerobic digestion centrate and municipal wastewater treatment via partial nitritation/anammox process: A promising approach to alleviate inhibitory effects and enhance nitrogen removal

Two-stage Partial nitritation/Anammox (PN/A) was firstly performed for recalcitrant organics (RO)-rich thermal hydrolysis pretreated anaerobic digestion (THP-AD) centrate treatment with municipal wastewater (MW) as co-substrate. Results indicated the inhibitory effects of RO was alleviated and high nitrate issue in PN/A effluent was addressed by cotreatment strategy. Stable PN with nitrite accumulation ratio of 95% and N removal efficiency of 97.1% were well maintained at MW of 80%. Nevertheless, nitrate accumulation and anammox activity loss were observed with lowering MW proportion owing to the weakened denitrification activity and aggravated inhibitory effect. Microbial analysis revealed Nitrosomonas was the major ammonium oxidizing bacteria and the ideal PN performance was due to the effective out-selection of nitrite oxidizing bacteria. Candidatus Kuenenia was identified as the primary bacteria for nitrogen removal (82.7%), and the controlled abundance of heterotrophic denitrifiers in anammox system ensured the enhanced nitrogen removal regardless of high COD loading from THP-AD centrate.

On anammox activity at low temperature: Effect of ladderane composition and process conditions

The application of partial nitritation-anammox (PN/A) under mainstream conditions can enable substantial cost savings at wastewater treatment plants (WWTPs), but how process conditions and cell physiology affect anammox performance at psychrophilic temperatures below 15 °C remains poorly understood. We tested 14 anammox communities, including 8 from globally-installed PN/A processes, for (i) specific activity at 10–30 °C, (ii) composition of membrane lipids, and (iii) microbial community structure. We observed that membrane composition and cultivation temperature were closely related to the activity of anammox biomasses. The size of ladderane lipids and the content of bacteriohopanoids were key physiological components related to anammox performance at low temperatures. We also indicate that the adaptation of mesophilic cultures to psychrophilic regime necessitates months, but in some cases can take up to 5 years. Interestingly, biomass enriched in the marine genus “Candidatus Scalindua” displayed outstanding potential for nitrogen removal from cold streams. Collectively, our comprehensive study provides essential knowledge of cold adaptation mechanism, will enable more accurate modelling and suggests highly promising target anammox genera for inoculation and set-up of anammox reactors, in particular for mainstream WWTPs.

Metagenomic Insights Into Competition Between Denitrification and Dissimilatory Nitrate Reduction to Ammonia Within One-Stage and Two-Stage Partial-Nitritation Anammox Bioreactor Configurations.

Anaerobic ammonia oxidizing bacteria (Anammox) are implemented in high-efficiency wastewater treatment systems operated in two general configurations; one-stage systems combine aerobic ammonia oxidizing bacteria (AOB) and Anammox within a single aerated reactor, whereas two-stage configurations separate these processes into discrete tanks. Within both configurations heterotrophic populations that perform denitrification or dissimilatory nitrate reduction to ammonia (DNRA) compete for carbon and nitrate or nitrite and can impact reactor performance because DNRA retains nitrogen in the system. Therefore, it is important to understand how selective pressures imposed by one-stage and two-stage reactor configurations impact the microbial community structure and associated nitrogen transforming functions. We performed 16S rRNA gene and metagenomic sequencing on different biomass fractions (granules, flocs, and suspended biomass) sampled from two facilities treating sludge dewatering centrate: a one-stage treatment facility (Chambers Creek, Tacoma, WA) and a two-stage system (Rotterdam, Netherlands). Similar microbial populations were identified across the different samples, but relative abundances differed between reactor configurations and biomass sources. Analysis of metagenome assembled genomes (MAGs) indicated different lifestyles for abundant heterotrophic populations. Acidobacteria, Bacteroidetes, and Chloroflexi MAGs had varying capacity for DNRA and denitrification. Acidobacteria MAGs possessed high numbers of glycosyl hydrolases and glycosyl transferases indicating a role in biomass degradation. Ignavibacteria and Phycosphaerae MAGs contributed to the greater relative abundance of DNRA associated nrf genes in the two-stage granules and contained genomic features suggesting a preference for an anoxic or microoxic niche. In the one-stage granules a MAG assigned to Burkholderiales accounted for much of the abundant denitrification genes and had genomic features, including the potential for autotrophic denitrification using reduced sulfur, that indicate an ability to adapt its physiology to varying redox conditions. Overall, the competition for carbon substrates between denitrifying and DNRA performing heterotrophs may be impacted by configuration specific selective pressures. In one-stage systems oxygen availability in the bulk liquid and the oxygen gradient within granules would provide a greater niche space for heterotrophic populations capable of utilizing both oxygen and nitrate or nitrite as terminal electron acceptors, compared to two-stage systems where a homogeneous anoxic environment would favor heterotrophic populations primarily adapted to anaerobic metabolism.

Insights into the mechanism of the deterioration of mainstream partial nitritation/anammox under low residual ammonium

Residual ammonium is a critical parameter affecting the stability of mainstream partial nitritation/anammox (PN/A), but the underlying mechanism remains unclear. In this study, mainstream PN/A was established and operated with progressively decreasing residual ammonium. PN/A deteriorated as the residual ammonium decreased to below 5 mg/L, and this was paralleled by a significant loss in anammox activity in situ and an increasing nitrite oxidation rate. Further analysis revealed that the low-ammonium condition directly decreased anammox activity in situ via two distinct mechanisms. First, anammox bacteria were located in the inner layer of the granular sludge, and thus were disadvantageous when competing for ammonium with ammonium-oxidizing bacteria (AOB) in the outer layer. Second, the complete ammonia oxidizer (comammox) was enriched at low residual ammonium concentrations because of its high ammonium affinity. Both AOB and comammox presented kinetic advantages over anammox bacteria. At high residual ammonium concentrations, nitrite-oxidizing bacteria (NOB) were effectively suppressed, even when their maximum activity was high due to competition for nitrite with anammox bacteria. At low residual ammonium concentrations, the decrease in anammox activity in situ led to an increase in nitrite availability for nitrite oxidation, facilitating the activation of NOB despite the dissolved oxygen limitation (0.15–0.35 mg/L) for NOB persisting throughout the operation. Therefore, the deterioration of mainstream PN/A at low residual ammonium was primarily triggered by a decline in anammox activity in situ. This study provides novel insights into the optimized design of mainstream PN/As in engineering applications.

Correction for Beach et al., "Exploring the Meta-regulon of the CRP/FNR Family of Global Transcriptional Regulators in a Partial-Nitritation Anammox Microbiome".

Correction for Beach et al., "Exploring the Meta-regulon of the CRP/FNR Family of Global Transcriptional Regulators in a Partial-Nitritation Anammox Microbiome".

Treating the filtrate of mainstream anaerobic membrane bioreactor with the pilot-scale sludge-type one-stage partial nitritation/anammox process operated from 25 to 15 °C

At ambient temperature condition, the one-stage partial nitritation/anammox (PNA) process has been successfully adopted to treat the filtrate from the mainstream anaerobic membrane bioreactor (AnMBR). However, there is no investigation of the performance of this process at low-temperature condition. In this study, the nitrogen removal performance of a pilot-scale PNA reactor at the temperature of 15 °C for treating the filtrate of a mainstream AnMBR was investigated. The nitrogen removal rate of 0.09 kg/m3/d and the nitrogen removal efficiency of 37.6% were achieved. The anammox reaction was the rate-limiting step of the nitrogen removal. Nitrogen removal was attributed in part to denitrification activity. The microbial community analysis confirmed that the main functional bacteria comprised of genus Nitrosomonas and genus Kuenenia. In sum, this research demonstrated the applicability of PNA process for mainstream AnMBR filtrate treatment to some extent and enriched the related knowledge.