Parenchyma Research Articles

Patient-Derived Tumor Organoids Combined with Function-Associated ScRNA-Seq for Dissecting the Local Immune Response of Lung Cancer.

In vitro models coupled with multimodal approaches are needed to dissect the dynamic response of local tumor immune microenvironment (TIME) to immunotherapy. Here the patient-derived primary lung cancer organoids (pLCOs) are generated by isolating tumor cell clusters, including the infiltrated immune cells. A function-associated single-cell RNA sequencing (FascRNA-seq) platform allowing both phenotypic evaluation and scRNA-seq at single-organoid level is developed to dissect the TIME of individual pLCOs. The analysis of 171 individual pLCOs derived from seven patients reveals that pLCOs retain the TIME heterogeneity in the parenchyma of parental tumor tissues, providing models with identical genetic background but various TIME. Linking the scRNA-seq data of individual pLCOs with their responses to anti-PD-1 (αPD-1) immune checkpoint blockade (ICB) allows to confirm the central role of CD8+ T cells in anti-tumor immunity, to identify potential tumor-reactive T cells with a set of 10 genes, and to unravel the factors regulating T cell activity, including CD99 gene. In summary, the study constructs a joint phenotypic and transcriptomic FascRNA-seq platform to dissect the dynamic response of local TIME under ICB treatment, providing a promising approach to evaluate novel immunotherapies and to understand the underlying molecular mechanisms.

Unravelling physiological and histochemical changes in two popular dessert and cooking bananas of India during fruit ripening

The physicochemical and histochemical characteristics of two consumingly divergent commercial varieties of Musaceae (Musa cv. Kanthali and Musa cv. Kacha Kela) were investigated during their postharvest ripening. The fruit peels from both cultivars were characterised by a reduction in chlorophyll contents, loosening of cortical tissues, and shrinkage of parenchymatous cells. The pulp-to-peel ratio, total soluble sugars, reducing sugar, fruit firmness, electrolyte leakage, and respiration rate were investigated along with activities of a few enzymes involved in fruit softening, namely cellulase, xylanase, invertase and glucanase. Additionally, semi-quantitative gene expression analyses with a few ripening-related genes, including ethylene biosynthesis (MaACS and MaACO), carbohydrate metabolism viz. sucrose synthase (MaSUS), sucrose phosphate synthase (MaSPS), glucanase (MaGLU), invertase (MaINV) and pectin methyl esterase (MaPME) were also carried out along with chitinase (MaCHT) in both cultivars during postharvest ripening. A faster postharvest ripening of ‘Kanthali’ than ‘Kacha Kela’ was evidenced as an outcome of the above studies.The peel histology and surface ultrastructure were examined through light microscopy and scanning electron microscopy (SEM), respectively. In light microscopy, substantial variations were noted between the cultivars, especially on peel-tissue degradation and accumulation of metabolites. The surface ultrastructure revealed temporal variations in the peel stomatal opening along with gradual depletion of surface wax. Such temporal opening of stomata was found to coincide with our earlier recorded postharvest volatiles emission patterns of respective cultivars suggesting it to be a legible vent for aroma emission.

Influence of nuclear factor κB and adenosine monophosphate-activated protein kinase on the vascular bed of the liver under the conditions of modeling chronic alcoholic hepatitis

Chronic alcohol use activates the transcription factor κB (NF-κB) in liver sinusoidal endothelial cells (LSECs), macrophages and other cells of the liver parenchyma, which controls the regulation of the expression of pro-inflammatory cytokines that activate signaling pathways of immune regulation of liver inflammation and vascular tone. AMP-activated protein kinase (AMPK) is an important immunometabolic regulatory factor in macrophages and, as a metabolic sensor, AMPK in vascular cells may be involved in the regulation of blood flow. The aim of the work was to find out the effect of modulators of the transcription factor κB and AMP-activated protein kinase on the morphometric parameters of the vascular bed of the liver of rats under the conditions of modeling chronic alcoholic hepatitis. Simulation of chronic alcoholic hepatitis leads to a violation of the exchange of metabolites between the central and interlobular veins, which creates prerequisites for the development of hypoxic damage to hepatocytes, as evidenced by a decrease in the diameters of lobular arterioles and venules. Administration of ammonium pyrrolidinedithiocarbamate and bacterial lipopolysaccharide, which affect NF-κB signaling under the conditions of modeling chronic alcoholic hepatitis, helps to restore the exchange of metabolites between the central and interlobular veins, which is evidenced by an increase in the lumens of lobular arterioles and venules. The introduction of phenformin and doxorubicin, which affect AMPK under the conditions of modeling chronic alcoholic hepatitis, prevents the development of hypoxic damage to hepatocytes, as evidenced by an increase in the diameters of lobular arteries and veins, and leads to intensification of interlobular blood circulation.

The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental study

The objective was to study the effect of the delivery of exogenous nitric oxide on the severity of apoptosis, pyroptosis, and necroptosis of the renal parenchyma after simulating cardiopulmonary bypass and cardiopulmonary bypass with circulatory arrest.Materials and Methods. 24 Altai breed rams were randomized into 4 equal groups. In the CPB and CPB+NO groups, we simulated cardiopulmonary bypass without circulatory arrest. In the CPB+CA and CPB+CA+NO groups, we simulated cardiopulmonary bypass with circulatory arrest. In the CPB+NO, CPB+CA+NO groups, NO was given perioperative in concentration of 80 ppm. In the CPB, CPB+CA groups, we supplied a standard oxygen-air mixture without NO.Results. In the CPB+CA+NO group, the TNF-α concentration was statistically significantly lower: 899 [739; 1019] ng/g compared to the CPB+CA group 1716 [1284; 2201] ng/g, p = 0.026. The remaining markers of programmed cell death did not differ between groups.Conclusions. Perioperative nitric oxide delivery reduces the expression of the extrinsic pathway of apoptosis of renal parenchyma cells in simulating operations with artificial circulation and circulatory arrest. Perioperative nitric oxide delivery at a dose of 80 ppm does not increase the processes of apoptosis, pyroptosis, and necroptosis in renal parenchyma.

Decoding dynamic bamboo cell shrinkage with time-lapse microscopy and machine-learning

The shrinkage of bamboo can be traced to the cellular scale; however, research on the shrinkage of bamboo cells’ scale is rare. This gap primarily arises from the intrinsic heterogeneity of biomass materials, leading to significant differences in the shrinkage behaviors of bamboo cells. Consequently, conventional measurement and analysis techniques struggle to obtain comprehensive and accurate results. To fill in this gap, this study investigated the dynamic shrinking behavior of individual bamboo cells, including fiber cells and parenchyma cells, using time-lapse microscopy and machine-learning techniques. Time-lapse observations revealed that the shrinkage rate of fiber cells across the cross-section surpassed that of parenchyma cells, accompanied by a greater shrinkage deformation. Specifically, fiber cells exhibited a shrinkage rate of 2.53 %/min, whereas parenchyma cells showed a rate of 0.58 %/min. This resulted in corresponding diameter reductions of approximately −30 % and −7 %, respectively. Additionally, machine-learning results demonstrated the efficacy of the trained long short-term memory (LSTM) model in accurately predicting morphological changes in individual parenchyma cells during the shrinkage process. This study advances understanding of bamboo-moisture interaction mechanisms and offers crucial theoretical data for improving bamboo drying and minimizing crack formation.

Dissecting Acute Drug-Induced Hepatotoxicity and Therapeutic Responses of Steatotic Liver Disease Using Primary Mouse Liver and Blood Cells in a Liver-On-A-Chip Model.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is hallmarked by hepatic steatosis, cell injury, inflammation, and fibrosis. This study elaborates on a multicellular biochip-based liver sinusoid model to mimic MASLD pathomechanisms and investigate the therapeutic effects of drug candidates lanifibranor and resmetirom. Mouse liver primary hepatocytes, hepatic stellate cells, Kupffer cells, and endothelial cells are seeded in a dual-chamber biocompatible liver-on-a-chip (LoC). The LoC is then perfused with circulating immune cells (CICs). Acetaminophen (APAP) and free fatty acids (FFAs) treatment recapitulate acute drug-induced liver injury and MASLD, respectively. As a benchmark for the LoC, multiplex immunofluorescence on livers from APAP-injected and dietary MASLD-induced mice reveals characteristic changes on parenchymal and immune cell populations. APAP exposure induces cell death in the LoC, and increased inflammatory cytokine levels in the circulating perfusate. Under FFA stimulation, lipid accumulation, cellular damage, inflammatory secretome, and fibrogenesis are increased in the LoC, reflecting MASLD. Both injury conditions potentiate CIC migration from the perfusate to the LoC cellular layers. Lanifibranor prevents the onset of inflammation, while resmetirom decreases lipid accumulation in hepatocytes and increases the generation of FFA metabolites in the LoC. This study demonstrates the LoC potential for functional and molecular evaluation of liver disease drug candidates.

Reproductive biology of Centaurea kilaea (Asteraceae, Cardueae) – an endemic species from Türkiye

In this study, the embryology of Centaurea kilaea Boiss., a species endemic to Türkiye, was examined using light microscopy. The anthers of C. kilaea are tetrasporangiate; the anther wall development is dicotyledonous; and the tapetum is amoeboid. The meiotic division of the microspore mother cells is regular, and when the pollen grains are thrown from the anthers, they are three-celled. The ovary of C. kilaea is inferior, bicarpellary, syncarpous, and unilocular, which is characteristic of the Asteraceae family. It carries only a single ovule with basal placentation. The ovule is anatropous, unitegmic, and tenuinucellate. The megaspore mother cell undergoes meiotic division, giving rise to a linear tetrad of megaspores. The chalazal megaspore remains functional, and the other three megaspores degenerate rapidly. The functional megaspore undergoes three mitotic divisions in succession. As a result, a Polygonum-type embryo sac, with eight nuclei and seven cells, is formed. The antipodal cells persist until the first divisions of the zygote. In the mature embryo sac stage, the integument consists of the endothelium, peri-endothelial region, parenchymatous cells, and outer epidermis, from the inside out. Endosperm development is initially free nuclear, becoming cellular in the globular embryo stage. Embryo development is of the asterad type. The mature seed does not contain endosperm, but the endothelium persists.

Combined ultra‐microelectrode: Exploring new potentials for in vivo/in situ ascorbic acid electroanalysis

AbstractMiniaturized and portable analytical tools show promise for sophisticated analysis, particularly in biological systems such as fruits, and they are suitable for advanced agriculture and related food industries. In this study, we developed combined ultra‐microelectrodes (UME) by modifying a microscale carbon fiber electrode (33 μm) coated with an Au nano‐film in a micropipette‐tip system. The proposed UME@Au exhibited a linear response to AC concentrations ranging from 30 to 1400 μM, with a 16 μM limit of detection. It demonstrated the ability to perform in vivo‐in vitro AC analysis in micro‐zones and volumes, such as different points of fruit tissue (Such as lemon) and within the body of a living plant (Such as Cactus arms and trunk), serving as a tiny implanted probe.In the first part of our study, we analyzed AC levels in lemon tissue directly. Our measurements revealed that AC levels are distributed heterogeneously in a single fruit. Additionally, stored AC levels depend on the color of the lemon (yellow ones have higher levels than the green ones). Furthermore, the UME was applied to control AC levels in different storage conditions, including opened containers, airtight containers, with and without exposing daylight, etc.In the second part, the UME@Au was utilized as an implanted sensor for in vivo analysis of AC in different parts of the cactus, recognized as a source of AC. No sample preparation is needed with minimum damage. The implanted microsensor could perform electroanalysis inside the live plant and stored parenchyma cells, etc. Notably, our results showed that AC levels are higher in the younger arms compared to the older ones, and so on.Based on our findings, the miniaturized, small, cheap, user‐friendly electrode demonstrated many capabilities, such as being implantable, having satisfactory stability, and not requiring sample preparations for analysis. It can open up a new window for micro‐electroanalysis in food and analytical plant sciences. We predict that this microscale platform can be modified and used for bioanalysis of other (bio)targets, such as vitamins, ions, and even the detection of plant pathogens in plants and crops directly. This involvement in the smart and modern farming industry is anticipated in the near future.

A clean, simple, and efficient method for preparing vibration-reducing low-carbon bamboo building materials with high strength

Large-format bamboo panels prepared by bamboo flattening are adhesive-free and retain the natural structure of bamboo, making them ideal materials for interior architecture. However, due to poor dimensional stability and vibration-reducing properties, flattened bamboo easily deforms and vibrates when applied to building walls and floors due to ambient humidity changes or external excitation, impacting both comfort and structural safety. In this work, a clean, efficient, and simple densification process for flattened bamboo is developed to prepare low-carbon bamboo building materials with enhanced vibration reduction. The results indicated that the densification process reduced the vibration intensity of the flattened bamboo under excitation, changed its vibrational modes, avoided low-frequency resonance, and improved its vibration reduction and suppression properties. The dynamic stiffness of the densified bamboo increased to 3.23–3.63 times, while the root mean square (RMS) and transfer function value decreased by 20.84 % and 62.5 %, respectively. The MOE (16.2 GPa) and MOR (276 MPa) increased to 2.73 times and 2.22 times, respectively. The densified bamboo met the dimensional stability requirements of the GB/T 18102–2020 building material standards. Compared with damping, the increased stiffness played the primary role in improving the vibration-reducing properties of densified bamboo. The hydrothermal mechanical treatment changed the structure and composition. From a macroscopic perspective, the gradient structure of the vascular bundles and parenchyma cells was gradually plasticized and homogenized, compacting large capillary pores and channels such as those in parenchyma cells, vessels, and sieve tubes. On a microscopic level, defects such as pits, intercellular spaces, and micropores were filled, and microfibrils between the middle lamellae were tightly stacked, forming a mechanically-interlocked structure. At the molecular scale, there was an increase in the crystallinity and orientation of cellulose molecular chains, accompanied by an increase in hydrogen bond density. The densified bamboo developed in this study shows good application potential for vibration-reduction structures such as buildings and rail transit.

Preparation of biomass activated carbon with aligned graphene nanosheet arrays from bamboo parenchymal cells for supercapacitors

Preparation of biomass activated carbon with aligned graphene nanosheet arrays from bamboo parenchymal cells for supercapacitors

Morphological, Physiological, Biochemical and Metabolite Analyses of Parenchyma Cells Reveal Heartwood Formation Mechanism of Schima superba

A sapwood tree is a species in which the sapwood does not differ significantly from the heartwood and cannot be classified by shades of color. It is generally accepted that heartwood has a higher economic value than sapwood, but most of the studies related to heartwood formation have focused on heartwood trees, with less research on sapwood trees. In this paper, we take the sapwood tree Schima superba as the research object and analyze the physiological and biochemical changes in the process of heartwood formation by studying the anatomical structure of parenchyma cells, and then further explore the main categories of metabolites and compositional changes. The results showed that during heartwood formation, the parenchyma cells become inactive and the nucleus disappears, while at the same time, the storage substance starch is gradually degraded under the action of enzymes and transformed into secondary metabolites, which include terpenoids, phenols and alkaloids. The accumulation of white and colorless compounds in large quantities in the heartwood, which has some effect on the heartwood color, is an important reason why the heartwood in Schima superba shows normal formation but no difference in color from the sapwood. This study fills a gap in the mechanism of heartwood formation in sapwood trees.

The OsZIP2 transporter is involved in root-to-shoot translocation and intervascular transfer of cadmium in rice.

Cadmium (Cd) is a toxic metal that poses serious threats to human health. Rice is a major source of dietary Cd but how rice plants transport Cd to the grain is not fully understood. Here, we characterize the function of the ZIP (ZRT, IRT-like protein) family protein, OsZIP2, in the root-to-shoot translocation of Cd and intervascular transfer of Cd in nodes. OsZIP2 is localized at the plasma membrane and exhibited Cd2+ transport activity when heterologously expressed in yeast. OsZIP2 is strongly expressed in xylem parenchyma cells in roots and in enlarged vascular bundles in nodes. Knockout of OsZIP2 significantly enhanced root-to-shoot translocation of Cd and alleviated the inhibition of root elongation by excess Cd stress; whereas overexpression of OsZIP2 decreased Cd translocation to shoots and resulted in Cd sensitivity. Knockout of OsZIP2 increased Cd allocation to the flag leaf but decreased Cd allocation to the panicle and grain. We further reveal that the variation of OsZIP2 expression level contributes to grain Cd concentration among rice germplasms. Our results demonstrate that OsZIP2 functions in root-to-shoot translocation of Cd in roots and intervascular transfer of Cd in nodes, which can be used for breeding low Cd rice varieties.

Bamboo as a natural optimized fiber reinforced composite: interfacial mechanical properties and failure mechanisms

Bamboo is a typical natural fiber-reinforced composite with an optimized distribution of vascular bundles as reinforcement and parenchyma tissues as bio-matrix. The interfacial bonding performance between vascular bundles and parenchyma tissue is critical for the mechanical properties and failure mechanisms of bamboo. This study employed pull-out tests to determine the interfacial shear strength (IFSS) between bamboo vascular bundles and parenchyma tissue and evaluate the critical embedded lengths () of vascular bundles. The effects of embedded vascular bundle lengths on interfacial strength and failure behaviors were also investigated. The results revealed a value of 2.51 mm, lower than the majority of plant fiber-reinforced composite materials, with an IFSS of around 20 MPa, surpassing most artificial bamboo fiber composites. The pull-out process of the vascular bundles involved elasticity, debonding, and sliding friction stage, where debonding energy absorption (DEA) outweighed frictional energy absorption (FEA) and increasing with embedded length. The primary failure features include interfacial debonding, parenchyma tissue ripping, delamination of fiber thin layers, and fiber breakage. Moreover, the parenchyma cells between the two fiber sheaths and at the top of the bamboo block readily detached. The interfacial failure mechanisms of bamboo included debonding, reinforcement, and matrix failure, with the proportions varying as the embedded length increased. Quantitative analysis of the interfaces structure and mechanical properties between vascular bundles and parenchyma tissues could provide a reference for the biomimicry of bamboo structures and the manufacturing of natural fiber-reinforced composite materials.

A genetically inducible endothelial niche enables vascularization of human kidney organoids with multilineage maturation and emergence of renin expressing cells

Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, organ mimicry, and ultimately transplantation hinge on achieving robust vascularization of in vitro engineered organs. Here, focusing on human kidney organoids, we overcame this hurdle by combining a human induced pluripotent stem cell (iPSC) line containing an inducible ETS translocation variant 2 (ETV2) (a transcription factor playing a role in endothelial cell development) that directs endothelial differentiation in vitro, with a non-transgenic iPSC line in suspension organoid culture. The resulting human kidney organoids show extensive endothelialization with a cellular identity most closely related to human kidney endothelia. Endothelialized kidney organoids also show increased maturation of nephron structures, an associated fenestrated endothelium with de novo formation of glomerular and venous subtypes, and the emergence of drug-responsive renin expressing cells. The creation of an engineered vascular niche capable of improving kidney organoid maturation and cell type complexity is a significant step forward in the path to clinical translation. Thus, incorporation of an engineered endothelial niche into a previously published kidney organoid protocol allowed the orthogonal differentiation of endothelial and parenchymal cell types, demonstrating the potential for applicability to other basic and translational organoid studies.

A reversal to hypogyny in Campanula alpina and its impact on capsule dehiscence: evidence from morpho-anatomy of the fruiting ovary

Introduction. Campanula alpina is a perennial herbaceous bellflower grown in the Eastern Alps, Carpathians, and Balkans. Fruit in Campanula species opens by the hippocrepiform slits in the fruit wall, which are formed by axicorns, elongated structures inside the ovary composed of lignified parenchyma cells. Our objectives were to reveal the fruit structure and dehiscence mode in C. alpina and compare it with C. latifolia, a type species of the genus Campanula. Materials and methods. Flowers and fruits at different stages of morphogenesis of C. alpina subsp. alpina were examined. Plant materials were sampled in two plots in the Chornohora massif of the Ukrainian Carpathians. Results. Contrary to other Campanula species, in C. alpina only the basal part of the ovary is fused with the calyx forming the flattened ovary base. The placentation is axile, the fruit wall and septas are parenchymatous, only axicorns are lignified. Dehiscence of the hanged fruit proceeds by the curving of axicorns outside by drying. In the dehiscent capsule viewed from above, hollow openings in the horizontal ovary base are exposed, and the curved axicorns attached to the crumpled fragments of the ovary wall are visible inside the ovary. Discussion. The dehiscence of the fruit in C. alpina proceeds through septifragal and hippocrepiform slits like in C. latifolia. The main differences are that the ovary is mostly superior, openings are located on the horizontal ovary base, and valves together with axicorns curve inside the ovary. The semi-inferior or nearly superior ovary insertion could be traced in illustrations in digital databases and researches for some taxa of Campanula section Medium. No issues have been made on the evolution of the ovary position in Campanuleae, since only the inferior ovary was referred for this tribe before the present article. The functional implications of shifts in the ovary position in C. alpina could be a result of selection of the pollination mechanism, or dissemination mode. Conclusions. The peculiarity of the fruit in C. alpina is the axicorns curving inside the ovary during the dehiscence. It is expected that deviation from the inferior ovary will be confirmed in other related species of the Campanula with pendent fruits. No argumentation about the evolution of secondary hypogyny in Campanula has been proposed so far.

Comparative analysis of foliar galls and ungalled leaves of Alstonia scholaris with a focus on tissue ultrastructure and phytochemistry

Plant-insect interactions where the insect changes the course of host-plant growth by building a new structure called as a “gall” to protect and nourish the growing insect continue to fascinate scientists. Alstonia scholaris, a medicinal plant extensively used in Indian Traditional Medicine has shown to have such interactions with insect Pauropsylla tuberculata Crawford producing numerous galls on the plant leaves. This intricate and poorly understood natural relationship demands further investigation to unravel the distinctions between gall tissue and unaffected leaves. We examined the tissue ultrastructure by scanning electron microscope (SEM) and phytochemistry by thin layer chromatography and liquid chromatography - high resolution mass spectrometry to understand variations between the gall tissues and ungalled leaves of A. scholaris. The data showed dense population of sclereids in the gall tissues in addition to parenchyma cells. The concentration of aluminium, phosphorus and carbon were found to be reduced in the galls while cobalt and oxygen were increased. The SEM images gives insight into the ecological relationship between these species. The phytochemical analysis indicated not only enrichment of alkaloids and terpenoids in the gall tissues but also observed several novel peaks with unreported masses indicating a potential reservoir of bioactive phytochemicals.

Pharmacognostical Evaluation, In-vitro Anti-Inflammatory and Anti-Arthritic Activities of Sageraea laurina Dalzell

Aim: Present investigation performed pharmacognostical evaluation, extraction, and evaluation of anti-inflammatory as well as anti-arthritic activities of Sageraea laurina Dalzell leaves. Materials and methods: Pharmacognostical evaluation was carried out for morphology and microscopy of Sageraea laurina Dalzell leaf and its powder, followed by determination of proximate values. Ethanolic (SLLE) as well as aqueous extract of leaves (SLLA) were evaluated for phytochemical screening, and in-vitro anti-inflammatory activity against MMP-2 (gelatinase-A) along with MMP-9 (gelatinase-B) by gelatin zymography as well as in-vitro anti-arthritic activity by egg albumin protein denaturation approach. Total phenolic as well as flavonoid content of extract were assessed. Percentage inhibition for protein denaturation was calculated. Results: Microscopical study of leaves showed presence of abaxial midrib, palisade parenchyma cells, phloem elements, xylem elements, spongy parenchyma, cystoliths, lithocysts, glandular trichomes, collenchyma cells thick lamina diacytic type of stomata and vessel elements. Phytochemical screening divulged existence of carbohydrates, flavonoids, steroids, alkaloids, saponin glycosides, tannins and phenolic compounds. Copious of phenolic compounds were noted in SLLE which were 89.42mg gallic acid equivalent/g (GAE/g dry weight) along 86.21mg QUE/g quercetin equivalent /g (QUE/g dry weight). Coomasie blue staining revealed proteolysis sites as translucent bands on dark blue background. Percentage inhibition for anti-inflammatory activity for SLLE against MMP-2 and MMP-9 was found to be 62% and 56% respectively, for SLLA against MMP-2 and MMP-9 was found to be 34% and 32% respectively. Egg albumin denaturation method, displayed percentage inhibition at concentration of 500µg/ml for SLLE and SLLA 74.14 and 60.30 respectively, standard drug diclofenac sodium at same concentration was found to be 84.53%. It was found that SLLE was more potent in inhibition of egg albumin denaturation than diclofenac sodium. Conclusion: Findings of present investigation revealed possession of significant in-vitro anti-inflammatory and antiarthritic activities by Sageraea laurina Dalzell leaves and serves as a natural biomedicinal remedial agent treating inflammation and rheumatism.

Proteomic lung analysis revealed hyper-activation of neutrophil extracellular trap formation in cases of fatal COVID-19

The molecular pathology of lung injury in patients with Corona Virus Disease 2019 (COVID-19) remain unclear. In this study, we performed a proteomic study of lung tissues from seven patients with COVID-19, and eight without. Lung parenchymal tissues with COVID-19 were obtained from autopsy samples, while control samples were obtained from paracancerous tissues. Proteins were extracted using phenol extraction. A tandem mass tag-based quantitative proteomic approach combined with bioinformatic analysis was used to detect proteomic changes in the SARS-CoV-2-infected lung tissues. A total of 6,602, and 6,549 proteins were identified in replicates 1 and 2, respectively. Of these, 307, and 278, respectively, were identified as differentially expressed proteins (DEPs). In total, 216 DEPs were identified in this study. These proteins were enriched in 189 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The downregulated proteins are mainly involved in focal adhesion (n = 5), and the PI3K-Akt signaling pathway (n = 4). The upregulated proteins were related to neutrophil extracellular trap (NET) formation (n = 16), and the phagosome pathway (n = 11). The upregulated proteins in these two pathways interact with one another. Further immunohistochemistry verified NET enrichment in the tissues with COVID-19 compared to the controls. Our results systematically outlined the proteomic profiles of the lung's response to SARS-CoV-2 infection and indicated that NET formation was hyper-activated. These results will hopefully provide new evidence for understanding the mechanism behind fatal COVID-19.

Sugar delivery at the tomato root and root galls after Meloidogyne incognita infestation

Root-knot nematodes (RKNs) infect host plants and obtain nutrients such as sugars for their own development. Therefore, inhibiting the nutrient supply to RKNs may be an effective method for alleviating root-knot nematode disease. At present, the pathway by which sucrose is unloaded from the phloem cells to giant cells (GCs) in root galls and which genes related to sugar metabolism and transport play key roles in this process are unclear. In this study, we found that sugars could be unloaded into GCs only from neighboring phloem cells through the apoplastic pathway. With the development of galls, the contents of sucrose, fructose and glucose in the galls and adjacent tissue increased gradually. SUT1, SUT2, SWEET7a, STP10, SUS3 and SPS1 may provide sugar sources for GCs, while STP1, STP2 and STP12 may transport more sugar to phloem parenchyma cells. At the early stage of Meloidogyne incognita infestation, the sucrose content in tomato roots and leaves increased, while the glucose and fructose contents decreased. SWEET7a, SPS1, INV-INH1, INV-INH2, SUS1 and SUS3 likely play key roles in root sugar delivery. These results elucidated the pathway of sugar unloading in tomato galls and provided an important theoretical reference for eliminating the sugar source of RKNs and preventing root-knot nematode disease.

Single-nucleus RNA and ATAC sequencing analyses provide molecular insights into early pod development of peanut fruit

Peanut (Arachis hypogaea L.) is an important leguminous oil and economic crop that produces flowers aboveground and fruits belowground. Subterranean fruit-pod development, which significantly affects peanut production, involves complex molecular mechanisms that likely require the coordinated regulation of multiple genes in different tissues. To investigate the molecular mechanisms that underlie peanut fruit-pod development, we characterized the anatomical features of early fruit-pod development and integrated single-nucleus RNA-sequencing (snRNA-seq) and single-nucleus assay for transposase-accessible chromatin with sequencing (snATAC-seq) data at the single-cell level. We identified distinct cell types, such as meristem, embryo, vascular tissue, cuticular layer, and stele cells within the shell wall. These specific cell types were used to examine potential molecular changes unique to each cell type during pivotal stages of fruit-pod development. snRNA-seq analyses of differentially expressed genes revealed cell-type-specific insights that were not previously obtainable from transcriptome analyses of bulk RNA. For instance, we identified MADS-box genes that contributes to the formation of parenchyma cells and gravity-related genes that are present in the vascular cells, indicating an essential role for the vascular cells in peg gravitropism. Overall, our single-nucleus analysis provides comprehensive and novel information on specific cell types, gene expression, and chromatin accessibility during the early stages of fruit-pod development. This information will enhance our understanding of the mechanisms that underlie fruit-pod development in peanut and contribute to efforts aimed at improving peanut production.