Parasitoids Hymenoptera Research Articles

Comparative Karyotype Analysis of Parasitoid Hymenoptera (Insecta): Major Approaches, Techniques, and Results.

A comprehensive review of main approaches, techniques and results of the chromosome study of parasitic wasps is given. In this group, the haploid chromosome number ranges from n = 3 to 23. Distribution of parasitic wasp species by the chromosome number is bimodal, with two obvious modes at n = 6 and 11. Karyotype analysis based on routinely stained preparations of mitotic chromosomes can be used to identify members of taxonomically complicated parasitoid taxa and to distinguish between them. Morphometric study effectively reveals subtle differences between similar chromosome sets of parasitic wasps. If combined with meiotic analysis and/or cytometric data, information on mitotic karyotypes can highlight pathways of the genome evolution in certain parasitoid taxa. C- and AgNOR-banding as well as staining with base-specific fluorochromes detected important interspecific differences within several groups of parasitic wasps. Fluorescence in situ hybridization (FISH) is successfully used for physical mapping of various DNA sequences on parasitoid chromosomes. These techniques demonstrate that heterochromatic segments are usually restricted to pericentromeric regions of chromosomes of parasitic wasps. Haploid karyotypes carrying one or two nucleolus organizing regions (NORs) are the most frequent among parasitoid Hymenoptera. In combination with chromosome microdissection, FISH could become a powerful tool exploring the genome evolution of parasitic wasps. Perspectives of the comparative cytogenetic study of parasitoid Hymenoptera are outlined.

Survey of potential parasitoids (Hymenoptera) of Drosophila suzukii (Diptera: Drosophilidae) in Buenos Aires province, Argentina

Drosophila suzukii (Matsumura) is a pest that affects a great variety of fruit crops. The objective of this work was to learn about the diversity of parasitoids (Hymenoptera) that might be associated with SWD in fruit crops located in northeastern Buenos Aires province, Argentina. Species were identified as Euxestophaga argentinensis Gallardo, Ganaspis pelleranoi (Brèthes), Leptopilina boulardi (Barbotin, Carton & Kelner-Pillault), Hexacola bonaerensis Reche (Cynipoidea, Figitidae), Trichopria anastrephae (Costa Lima) (Diaprioidea, Diapriidae) and Pachycrepoideus vindemmiae (Rondani) (Chalcidoidea, Pteromalidae). Leptopilina boulardi, E. argentinensis and T. anastrephae were reported for the first time in the Buenos Aires province.

Lack of lipid accumulation in two species of chalcidoid wasps with secondarily evolved phytophagy

AbstractStable co‐evolutionary relationships between species may result in the loss of autonomous synthesis of essential nutrients when these can be obtained from the ecological partner. Parasitoid insects obtain most of their nutrients from their host, and many lineages do not increase their adult lipid reserves even when feeding on a surplus of sugars. Several clades within the parasitoid Hymenoptera have lost the parasitoid lifestyle and switched to either a predatory or a phytophagous lifestyle. Here, we test whether adult lipid accumulation is reactivated in two species that independently evolved a phytophagous lifestyle from parasitoid ancestors. The larvae of Megastigmus aculeatus (Swederus) (Hymenoptera: Chalcidoidea: Torymidae) and Bruchophagus platypterus (Walker) (Hymenoptera: Chalcidoidea: Eurytomidae) obligatorily feed on seeds of specific plants. Seeds of wild host plants were collected and the emerging wasps were tested for the lipid accumulation phenotype using whole‐body diethyl ether extraction. Both species had significantly reduced lipid content after 1 week ad libitum access to sugars compared to the lipid content at emergence, indicating a lack or negligible level of lipogenesis. Therefore, the secondary switch to phytophagy was not associated with recovery of lipid accumulation in these species. We discuss these results in relation to the lipid content of the host seeds and the reversibility of the lipid accumulation phenotype. We infer that the close relationship among seed‐feeding insects and their hosts allows the seed‐feeders to rely on a diet with predictable lipid quantities which relaxes the need for autonomous lipid synthesis.

Role of Egg Parasitoids in Controlling the Pine Processionary Moth in the Cedar Forests of Chréa National Park (Algeria)

The sustainable protection of cedar stands in Chréa National Park can only be accomplished through the stability of the ecosystem. Outbreaks of Thaumetopoea pityocampa are a major threat and are largely attributed to the high population fecundity, changes in the diversity of natural enemies and global interactions within the ecosystem. Egg parasitoids (Hymenoptera) are essential in the biological control of T. pityocampa. To assess the impact of the parasitoids on the populations of the pine processionary moth, egg masses from cedar plantations were collected, reared in a laboratory and checked regularly for the emergence of the egg parasitoids: Trichogramma embryophagum, Baryscapus servadeii and Ooencyrtus pityocampa. Observations showed an inter-annual variation in the abundance of the three parasitoids as a result of the variation in the population density of the processionary moth, and on the underlying effect of temperature. Parasitoids had variable parasitism rates, with yearly averages ranging from 3.86% to 51.14%, dependent on the spatiotemporal distribution of the host populations. The aggregate effect of multiple parasitoid species could optimize control of T. pityocampa in cedar stands.

Natural Enemies and Pest Control in Field-Grown Crop in Southern Senegal

Crop pests are a permanent threat to horticultural production. Several control methods are recommended for their control, including biological control. This method based on the use of natural enemies is not well established. The objective of this study was to assess the diversity and abundance of natural enemies of crop pests. A survey of crops associated with pests was carried out on 144 fields in southern Senegal to measure the diversity and abundance of natural enemies of various crop pests, determine the native parasitoid complex and assess parasitism rates. Ecological indices were calculated to assess the diversity of natural enemies. A total of 25 natural enemy species were identified, including 15 parasitoids and 10 predators. Predatory Hemiptera and Parasitoid Hymenoptera were high biodiversity. The parasitism rates vary between 0 and 50% depending on the abundance of pests and parasitoid species. Knowledge of the diversity of natural enemies and the understanding of trophic interactions with pests are important in the development of biological crop protection in order to preserve resilience in agroecosystems.

Study of the interaction between the olive tree pests and their natural enemies

The aim of this investigation was to study the interaction between the olive pests and their natural enemies for the establishment of possible control programs and prevention against the main pests of olive trees and to ensure the agro-ecological stability of olive orchards and their protection. Using several sampling techniques, presence of four pests and five predators was observed on the olive tree. The phytophagous insects included serious olive tree pests; Bactrocera oleae (Rossi) (Diptera: Tephritidae), Euphyllura olivina (Costa) (Hemiptera: Psyllidae), Liothrips oleae Costa (Thysanoptera: Phlaeothripidae) and Parlatoria oleae (Colvée) (Hemiptera: Diaspididae). Significant positive correlations were acquired between the presence of the predators Chrysoperla carnea and Anthocoris nemoralis with the four pests of the olive tree. The presence of the predators, Scymnus marinus, Scymnus subvillosus and Nesidiocoris tenuis was insignificant compared to the olive pests. The activity of Hymenoptera parasitoids such as Ephedrus plagiator, Bracon sp., Encyrtidae sp. and Diglyphus sp. is necessary for the populations of B. oleae and E. Olivina.

Multiple Data Demonstrate That Bacteria Regulating Reproduction Could Be Not the Cause for the Thelytoky of Diglyphus wani (Hymenoptera: Eulophidae).

Simple SummaryIn Hymenoptera, some species have two reproduction modes, that is, arrhenotokous and thelytokous parthenogenesis. Although arrhenotoky is common, the thelytoky is not rare. Thelytoky in Hymenopteran species has several causes and is generally induced by bacteria. Here, we examined a thelytokous Chalcidoidea parasitoid, Diglyphus wani, which was recently reported to be a dominant parasitoid attacking agromyzid leafminers. In this study, we aimed to combine multiple methods to explore whether endosymbionts induce thelytokous D. wani. Our findings revealed that the thelytoky of D. wani was not to be associated with the actual presence of the endosymbionts. Thus, Diglyphus wani is the second reported eulophid parasitoid for which thelytoky is not linked with the presence of endosymbionts.In Hymenoptera parasitoids, the reproductive mode is arrhenotoky, while a few species reproduce by thelytoky. The thelytoky of Hymenoptera parasitoids is generally genetically determined by the parasitoids themselves or induced by bacteria, including Wolbachia, Cardinium, and Rickettsia. Diglyphus wani (Hymenoptera: Eulophidae), a recently reported thelytokous species is a main parasitoid attacking agromyzid leafminers. To assess whether endosymbionts induce thelytoky in D. wani, we performed universal PCR detection and sequenced the V3-V4 region of 16S ribosomal RNA gene. In addition, bacteria were removed through high-temperature and antibiotic treatments, and the localized bacteria were detected using FISH. Based on general PCR detection, Wolbachia, Cardinium, Rickettsia, Arsenophonus, Spiroplasma, and Microsporidia were absent in laboratory and field individuals of thelytokous D. wani. Furthermore, 16S rRNA gene sequencing revealed that the dominant endosymbionts in thelytokous D. wani were not reproductive manipulators. High-temperature and antibiotic treatment for five consecutive generations cannot reverse the thelytokous pattern of D. wani, and no male offspring were produced. Moreover, no bacterial spots were found in the ovaries of D. wani. Thus, it is considered that the thelytoky of D. wani does not result in the presence of endosymbionts. This species is thus the second reported eulophid parasitoid whose thelytoky appears not to be associated with endosymbionts.

First occurrences of the host/parasitoid relationship in Brazil and Peru: Bibliographic summary

It is common for parasitoidism to modify the behavior of the host species, facilitating the development and propagation of the parasitoid and establishing a highly specific relationship. In this case, herbivores such as cattle, sheep, deer, and rabbits are the definitive host of the parasitoid. The objective of this review is to describe the first occurrence of the host/parasitoid relationship in Brazil and Peru. The mini review consists of a bibliographic summary of parasitoids of the Order Hymenoptera parasitoids collected in Brazil and Peru. The research was carried out in studies related to the theme with emphasis on the quantitative aspects of the Superfamily, Family, Subfamilies, Genera, and Species (taxonomic groups). A literature search was carried out containing articles published from 2000 to 2021. The mini review was prepared in Goiânia, Goiás, from July to September 2021, using the Electronic Scientific Library Online (Scielo) and internet. Since the relationship between parasitoids and their hosts is very specific, it is common for agricultural pests to be naturally controlled through parasitoidism. Wasps of the Ichneumonoidea and Braconidae family respectively parasitize caterpillars of butterflies and moths, and even aphids.

Mini review of parasitoids collected in biomes and morphoclimatic domains in Brazil

In Brazil we can find six types of biomes: Amazon, Atlantic Forest, Cerrado, Caatinga, Pampa, Pantanal, and two morphoclimatic domains: the Araucaria Forest the Mata from Cocais. Our biomes and morphoclimatic domains are important not only as natural resources in our country but stand out as environments of great natural wealth on the planet. The aim of this study is to describe the parasitoids collected in Brazilian biomes. The mini review consists of a bibliographic summary of parasitoids of the Order Hymenoptera parasitoids collected in the following Biomes and morphoclimatic domain in Brazil. The research was carried out in studies related to the theme with emphasis on the quantitative aspects of the superfamily, family, subfamilies, genera, and species (Taxonomic groups). A bibliographic search was carried out that contained papers published from 2006 to June 2021.The mini review was prepared in Goiânia, Goiás from March to June 2021using the Electronic Scientific Library Online (Scielo) and internet. This biodiversity can offer unique opportunity for pest control in both Brazil and other countries, due to the identification of new organisms with potential to be used in biological control.

Hubungan antara struktur lanskap dan keanekaragaman Hymenoptera parasitoid: Sebuah model interaksi pada pertanaman kacang panjang

The structure of agricultural landscape, both of its composition and configuration can affect the presence of predators and parasitoids, and influence the effectiveness of biological control. This study was aimed to examine the relationship between landscape structure and the diversity of Hymenoptera parasitoids in long bean fields in Bogor, West Java. In total, six long bean fields were selected and quantified within a radius 500 m from long bean field. In each long bean field, Hymenoptera parasitoids were sampled in a 50 m x 25 m plot using direct observation. The research result found 283 individuals of Hymenoptera parasitoids belong to 9 families and 31 species. Based on the Mantel test, the landscape structure did not affect the species composition of parasitoids in long bean. However, at class scale, landscape composition especially crop field and semi-natural habitat have an effect on parasitoid abundance. Agricultural landscape with higher patch number of crop fields tends to have higher parasitoid abundance. It indicates that landscape composition at class scale is more affected to Hymenoptera parasitoids than at landscape scale.

Sampling methods and metereological factors on pests and beneficial organisms in strawberries

We characterize the population dynamics of pests and beneficial organisms in strawberries to assess the impact of two meteorological factors (temperature and precipitation) and define the most appropriate method for monitoring insects in strawberries crop. Population dynamics were monitored weekly using the plant beat method and Möericke traps in Albion strawberries. Measurements were taken in organic strawberry farms over two growing seasons, from March 26, 2010 to August 23, 2010 (first season - 2009/2010) and from October 4, 2010 to March 21, 2011 (second season - 2009/2010) in the municipality of Pinhais, Paraná state, southern Brazil (25º25'S; 49º08'W). We collected a total of 7,971 pests and 1,987 beneficial organisms. The most abundant pests were Chaetosiphon fragaefolii (Cockerell) (Hemiptera: Aphididae) in the first growing season and Tetranychus urticae (Koch) (Acari: Tetranychidae) in the second. Beneficial organisms from Acari, Araneae, and Hymenoptera parasitoids predominated in both seasons. Temperature and precipitation were the the primary climatic factor associated with the population variation of aphids. Populations of C. fragaefolii grew when the temperature was between 16°C and 25ºC. We found that plant beat was the fastest and most suitable method for strawberry pests monitoring since it captured insects that were effectively colonizing the plants. This study contributes to a better understanding of the entomofauna associated with cultivation of organic strawberry.

Synthesis of the interactions between the European species of the genus Bruchus (Coleoptera: Chrysomelidae: Bruchinae) and their natural enemies

Summary Scientific literature has been studied to determine the known relationships between the European species of the genus Bruchus and their natural enemies, restricted to the arthropods. Firstly, an overview of the studies concerning natural enemies of the bruchid beetles is provided. Then, an analysis of the known relationships is given: 48 natural enemy species associated with Bruchus are highlighted, with a majority of Hymenoptera parasitoids. Relationships are commented and their validity is assessed. Lastly, a lack of knowledge of most species is indicated by this study.

Hymenoptera Parasitoid dan Persentase Parasitasi terhadap Berbagai Ulat Pemakan Daun Kelapa Sawit di Kebun Cikasungka PTPN VIII, Cindali, Bogor

Palm oil is one of the plantation commodities in Indonesia and had widespread in Indonesia. One of pests that attack oil palm is insect, and the most damaging pests usually from Lepidoptera. The aim of this study was to determine and counting percent parasitization of the parasitoids that parasitize palm leaf-eating pests in the oil palm plantation of PTPN VIII Cindali, Bogor. The study was conducted in 6 blocks of PTPN VIII Cindali oil palm plantation from September 2014 - June 2015 with observations once a month. Sample was taken by means of each block taken 5 plants in every plots randomly to be observed and palm leaf-eating insects taken to kept until the parasitoid was emerged. The outgoing parasitoids then counted and identificated. The results of the study founds 6 species of palm leaf-eating pests and 5 species of Hymenoptera parasitoids that parasitize these pests. Pests that found were Setora nitens , Amatissa sp., Birthosea bisura , Mahasena corbetti , Metisa plana and Pseudococcus sp. Whereas parasitoids that found were Spinaria spinator , Charops bicolor , Telenomus podisi , Aphanogmus sp. and Acerophagus sp.

Abundance, not diversity, of host beetle communities determines abundance and diversity of parasitoids in deadwood.

Most parasites and parasitoids are adapted to overcome defense mechanisms of their specific hosts and hence colonize a narrow range of host species. Accordingly, an increase in host functional or phylogenetic dissimilarity is expected to increase the species diversity of parasitoids. However, the local diversity of parasitoids may be driven by the accessibility and detectability of hosts, both increasing with increasing host abundance. Yet, the relative importance of these two mechanisms remains unclear. We parallelly reared communities of saproxylic beetle as potential hosts and associated parasitoid Hymenoptera from experimentally felled trees. The dissimilarity of beetle communities was inferred from distances in seven functional traits and from their evolutionary ancestry. We tested the effect of host abundance, species richness, functional, and phylogenetic dissimilarities on the abundance, species richness, and Shannon diversity of parasitoids. Our results showed an increase of abundance, species richness, and Shannon diversity of parasitoids with increasing beetle abundance. Additionally, abundance of parasitoids increased with increasing species richness of beetles. However, functional and phylogenetic dissimilarity showed no effect on the diversity of parasitoids. Our results suggest that the local diversity of parasitoids, of ephemeral and hidden resources like saproxylic beetles, is highest when resources are abundant and thereby detectable and accessible. Hence, in some cases, resources do not need to be diverse to promote parasitoid diversity.

Moderately decreasing fertilizer in fields does not reduce populations of cereal aphids but maximizes fitness of parasitoids

Examination of the tradeoff between the extent of decreasing nitrogen input and pest suppression is crucial for maintaining the balance between essential yield and an efficient, sustainable pest control strategy. In this study, an experiment with four manipulated nitrogen fertilizer levels (70, 140, 210, and 280 kg N ha−1 = conventional level) was conducted to explore the effects of decreasing nitrogen on cereal aphids (Sitobion avenae and Rhopalosiphum padi) (Hemiptera: Aphididae), Aphidiinae parasitoids (Hymenoptera: Braconidae: Aphidiinae), and body sizes of parasitoids. The results indicated that nitrogen application, in the range of 70–280 kg N ha−1, has the potential to impact the populations of cereal aphids and their parasitoids. However, both differences between densities of cereal aphids and their parasitoids in moderate (140–210 kg N ha−1) and those in high nitrogen input (280 kg N ha−1) were not significant, and the parasitism rate was also unaffected. A higher parasitism rate reduced population growth of the cereal aphid (S. avenae). Additionally, a moderate decrease of nitrogen fertilizer from 280 to 140–210 kg N ha−1 maximized the body sizes of Aphidiinae parasitoids, indicating that a moderate decrease of nitrogen fertilizer could facilitate biocontrol of cereal aphid by parasitoids in the near future. We conclude that a moderate decrease in nitrogen application, from 280 to 140–210 kg N ha−1, does not quantitatively impact the densities of cereal aphids or the parasitism rate but can qualitatively maximize the fitness of the parasitoids.

Damage Caused by Seed-Feeding Insects in Senna corymbosa: A New Host Plant for the Subfamily Bruchinae

In the larval stage, bruchines can cause several damages to the seeds of their host plants due to the consumption of the embryo. The aim of this study was to identify the species of seed-feeding insects in Senna corymbosa (Fabaceae) and quantify the damage caused to seeds. For this purpose, ripe fruits of S. corymbosa were collected monthly from May to August 2014. The fruits were stored in containers to obtain the adult insects and quantify the damage to the seeds. A total of 3,548 seed beetles emerged from the fruits, around 89% belonging to Sennius lateapicalis. Insects consume up to 43.2% of the internal seed content. Moreover, the seed beetles Hymenoptera parasitoids emerged. In this study, seed-feeding insects are recorded for the first time in S. corymbosa. In addition, it contributes to describing fruits and seeds, as well as associated bruchine species and the damage they cause to seeds.

First record of the association of a species of Lycaenidae (Lepidoptera) with Zornia latifolia Sm. (Fabaceae), and its parasitoid (Hymenoptera: Chalcididae) in Brazil

The family Lycaenidae (Lepidoptera) comprises over 6,000 described and widely distributed species. However, studies on interactions with other insects such as ants, parasitoids, and with food plants in the Neotropical region, are still scarce, even though such information are fundamental for better understanding the natural history of this taxonomic group. This study reports a new food plant to larvae of Lycaenidae species in the neotropics, as well as its parasitoid. A Lycaenidae larva was found and collected for immature stage observation under laboratory conditions. The larva fed on petals and seeds of Zornia latifolia Sm. (Fabaceae). Nineteen days after pupation in laboratory a larvipupal parasitoid of the genus Conura (Chalcididae) had egressed. This is the first report of tritrophic relationship amongst Z. latifolia, a Lycaenidae larva and its larvipupal parasitoid of the genus Conura in a periurban area near remnants of the Atlantic Forest, in Northeastern Brazil.

Sampling of parasitoid Hymenoptera: influence of the height on the ground

Parasitoid hymenopterans are a highly diverse group of insects; therefore, the choice of an adequate sampling method becomes important to achieve a representative species richness of a site. The aim of this work is to evaluate the size and diversity of parasitoids in relation to the height of the Malaise trap placement above the ground of a low deciduous forest from Yucatan, Mexico. Parasitoids were collected from September to October 2015, using three Malaise traps at ground level and other three located right above the others, leaving no space between them, at a height of 1.5 m. The collected specimens were identified at family level. A total of 4083 parasitoids belonging to 31 families were collected, representing 93% of the sample’s completeness, according to Jack 1 estimator; with differences in richness and abundance between trap heights according to rarefaction and fixed effects multifactorial ANOVA, respectively. Bethylidae, Braconidae and Ichneumonidae were the most abundant families. Besides, when analyzing the differences of each family by separate, there were significant results for Bethylidae, Diapriidae and Ichneumonidae with more individuals in the traps at ground level than in the raised ones. In a further analysis, the effect of body size on the capture height was observed. The specimens of larger size belonging to the families Bethylidae, Sphecidae, Sclerogibbidae and Evaniidae were more collected at ground level, on the other hand, the larger sized Ichneumonidae were collected at raised level.

The The diversity and abundance of Hymenoptera insects on tidal swamp rice field in Indragiri Hilir District, Indonesia

Abstract. Ikhsan Z, Hidrayani, Yaherwandi, Hamid H. 2020. The diversity and abundance of Hymenoptera insects on tidal swamp rice field in Indragiri Hilir District, Indonesia. Biodiversitas 21: 1020-1026. Hymenoptera is one of the four largest insect orders and it plays a vital role in human life as pollinators, parasitoids, and predators. Hymenoptera parasitoids are particularly important as agents of biological control of pest. The objective of this research was to study the diversity and abundance of Hymenoptera on tidal swamp rice field in Indragiri Hilir District, Riau Province, Indonesia. Samplings were conducted in 3 sub-districts of rice production centers, namely Batang Tuaka, Keritang and Reteh, using four trapping techniques (i.e. insect net, malaise trap, pitfall trap, and yellow pan trap). Shannon diversity index (H '), Krebs evenness index (E), and Jaccard similarity index (Is) were calculated. We found 4,701 individuals consisting of 39 families and 319 species of Hymenoptera at the three studied areas. Tidal swamp rice in Indragiri Hilir District had a high species diversity of Hymenoptera parasitoids and Hymenoptera predators, but a moderate species diversity of Hymenoptera pollinators. Species diversity and evenness of Hymenoptera parasitoids and predators were higher in Keritang than in Batang Tuaka and Reteh. The families of Formicidae, Braconidae, Ichneumonidae, and Scelionidae had the highest number of species, while Formicidae, Scelionidae, Diapriidae, and Braconidae had the highest number of individuals. Based on these findings, the abundance and diversity of Hymenoptera insects in Indragiri Hilir District have the potentials to be developed as biological agents to control pests on tidal swamp rice in this area.

Tipe penggunaan lahan memengaruhi keanekaragaman dan komposisi hymenopteran parasitoid di Jambi

Hymenoptera merupakan salah satu dari empat ordo serangga terbesar di dunia yang salah satu perannya adalah sebagai musuh alami (parasitoid, predator). Konversi lahan dapat memengaruhi keanekaragaman dan komposisi hymenopteran parasitoid dan peran ekologisnya. Penelitian ini bertujuan untuk memelajari pengaruh tipe penggunaan lahan terhadap keanekaragaman dan komposisi dari hymenopteran parasitoid di Jambi. Penelitian diakukan pada berbagai tipe penggunaan lahan di lanskap Hutan Harapan dan Taman Nasional Bukit Duabelas (TNBD), Provinsi Jambi meliputi hutan, hutan karet, perkebunan kelapa sawit, dan perkebunan karet. Pengambilan sampel serangga dilakukan dari bulan Maret sampai September 2013, dengan teknik pengasapan (fogging). Sebanyak 14.258 individu hymenopteran parasitoid yang terdiri atas 30 famili diperoleh dari seluruh lokasi penelitian. Famili Encyrtidae, Bracondae, Aphelinidae, Eulophidae, Scelionidae, Ceraphronidae, dan Platigasteridae merupakan famili yang ditemukan dengan kelimpahan tinggi. Tipe penggunaan lahan kelapa sawit ditemukan memiliki komposisi parasitoid berbeda dibandingkan dengan tipe penggunaan lahan lainnya, sedangkan tipe penggunaan lahan hutan dan hutan karet memiliki komposisi spesies yang tinggi. Hasil penelitian ini menunjukkan bahwa tipe penggunaan lahan mempengaruhi keanekaragaman dan komposisi spesies hymenopteran parasitoid.