Parasitic Illnesses Research Articles

384. National Trends and Racial Disparities in Sepsis-Related Mortality in Children in the United States, 1999-2019

Abstract Background There is conflicting data on the racial differences in sepsis-related mortality rate (SRMR) in children and little is known about the trends in SRMR in children in the United States (US). We examined the national trends and racial disparities in SRMR in children in the US from 1999 to 2019. Sepsis-related mortality rate in US children stratified by age, gender, race, and geographic region. Sepsis-related mortality rate in US children stratified by age, gender, race, and geographic region. Methods This was a retrospective cross-sectional analysis of national death certificate data from the CDC WONDER database from 1999-2019. We used ICD-10 codes to identify children aged 0-18 years who had bacterial sepsis as the underlying cause of death. We excluded deaths from viral, fungal, parasitic, tuberculous, and rickettsial illnesses. The crude SRMR was calculated per 100,000 population and further stratified by age, gender, race, and census region. Racial disparity was evaluated by the NHB-to-NHW SRMR ratio. The Mann-Whitney U test was used to compare two groups and P < 0 .05 defined statistical significance. We evaluated temporal trends with Joinpoint regression, expressed as average annual percentage change (AAPC) with 95% confidence intervals (CI). Next, we repeated the analysis with any mention of bacterial sepsis as a cause of death.Figure 1.Trends in sepsis-related mortality rate in children in the US, 1999-2019. Results Among 1.6 billion children, 23,466 had bacterial sepsis as the underlying cause of death [1.4 per 100,000; CI, 1.4 -1.5)]. The SRMR differed by age, gender, race, and geographic region (Table 1). The overall SRMR decreased from 1.7 in 1999 to 1.2 in 2019 (AAPC -1.9%; CI: -2.4, -1.3%) [Figure 1]. The SRMR decreased in males (1.9 to 1.2; AAPC -2.2%, CI: -2.5, -1.9), females (AAPC -2%, CI: -2.5, -1.6), NHB (AAPC -1.7%, CI: -2.3, -0.9); NHW (AAPC -2.1%, CI: -2.4, -1.6), rural (AAPC -2.5%, CI: -3.4, -1.5) and urban areas (AAPC -1.9%, CI: -2.3, -1.5), and in all census regions. The overall NHB-to-NHW SRMR ratio was 2.7 and there was no significant change during the study period. When limited to any mention of sepsis as the cause of death, the differences and trends in SRMR remained unchanged (Figure 1B). Conclusion The SRMR declined significantly overall and for all demographic groups. The persistent disparity in SRMR between NHB and NHW suggests differential access to care, recognition and prompt treatment of sepsis. Future studies should unravel the underlying causes of this disparity in mortality outcomes. Disclosures All Authors: No reported disclosures

Platinum Group Metals against Parasites: State of the Art and Future Perspectives

Background: Globally, parasitic diseases are considered among the neglected diseases. Clinically, several drugs are used in treatment, however due to drug resistance and multidrug resistance and the low investment in new research lines, there has been a failure in the treatment of parasitic illnesses. Objectives: The present mini-review is a comprehensive review of the use of platinum group metals as biological agents. It aims to establish the actual state of the art of these metal elements in the antiparasitic activity-specific area and define the future possibilities of action. Methods: The review comprises more than 100 research works done in this field. The differences between platinum group metals chemistry and their use as metal complexes with biological activity have been discussed. Results: This review highlighted the platinum group metal's potential as an antiparasitic agent for different diseases. Conclusion: The review will be helpful for the researchers involved in targeted drugs for parasitic disease therapy.

In-vivo implementation of the HPTLC methodology to rat plasma for the concurrent determination of a novel combination therapy for the management of COVID-19 (favipiravir and nitazoxanide).

The 2019 coronavirus outbreak has prompted scientists to investigate pharmaceuticals to prevent the spread of the disease. Favipiravir (FAV) has received Food and Drug Administration FDA approval for the treatment of various viral infections with notable efficacy in clinical trials for COVID-19. Nitazoxanide (NTZ) is a broad-spectrum antiparasitic and antiviral agent used for the treatment of parasitic illnesses. Recently, the antiviral medications FAV and NTZ have been utilized therapeutically as early antiviral combination therapy for COVID-19, highlighting their safety, efficacy, and immunomodulatory effects. Despite several clinical studies on both FAV and NTZ, no analytical method has been developed for their concurrent detection. The objective of this study is to develop a TLC-densitometric method for their assessment and application to rat plasma. FAV, NTZ, and tizoxanide (the active metabolite of nitazoxanide (TZM)) were simultaneously determined using an HPTLC method embracing sulfasalazine as an internal standard (IS). Silica gel 60 F254 used as the stationary phase for chromatographic separation, the mobile phase consisted of chloroform-methanol-formic acid (9.5:0.5:0.2, v/v/v), with UV detection at 230 nm demonstrating linearity within the range of 0.5-5 μg/band. The proposed methodology has been shown to effectively measure the analyzed elements in both pure form and in-vivo rat plasma.

Combined echinococcosis of the lungs, heart and liver: clinic, diagnosis and treatment. Case report

Echinococcosis or hidatid disease is a parasitic illness which is caused by the most common pathogens Echinococcus granulosus, E. multilocularis and E. oligarthrus. When the agent gets into the organism, it penetrates the organ and forms a cyst. Cysts are located more often (75%) in the liver where they exist without any clinical manifestation. They can be located in other internal organs: lungs, the heart and others. The localization of cysts in several organs simultaneously is a very rare case. The article aims to demonstrate a patient who had a combined echinococcosis of the lungs, heart and liver.

Galectin-3 plays a key role in controlling infection by Toxoplasma gondii in human trophoblast cells and human villous explants.

Galectin-3 (Gal-3) is a β-galactoside-binding lectin expressed in cells of the placental microenvironment. This lectin is involved in various biological processes, such as modulation of the immune system and control of parasitic illness. Toxoplasma gondii infection can lead to congenital transmission and cause miscarriages, prematurity and fetal anomalies. However, little is known about the role of Gal-3 in T. gondii infection in the placental microenvironment. This study aimed to unravel the underlying mechanisms of Gal-3 during T. gondii infection. For this purpose, we promoted the knockdown of Gal-3 expression by using RNA interference (RNAi) in BeWo cells or by using a synthetic inhibitor (GB1107) in human villous explants. We showed that the decreased Gal-3 expression in BeWo cells and human villous explants increases the invasion and proliferation of T. gondii probably by downregulating MIF and IL6 levels, highlighting thus the role of this lectin in modulating the immune response. Collectively, our study reveals Gal-3 as a promising target protein during congenital toxoplasmosis.

Importance of tailored non-clinical safety testing of novel antimalarial drugs: Industry best-practice

Malaria is an acute, debilitating parasitic illness. There were 249 million cases of malaria in 2022, resulting in 608,000 deaths globally, 76% of which were children ≤5 years. The unique nature of this disease (recurrences leading to re-treatments and numerous organ systems affected), specific clinical treatment regimens, poor compliance, and diversity of affected populations (predominantly pediatrics, women of childbearing potential, pregnant and lactating women), often makes standard testing approaches inadequate, and tailor-made safety assessments are more appropriate.We provide best practice recommendations based on company experience for the non-clinical safety assessment of antimalarial drugs, with a focus on small molecules since they represent the majority of drug candidates for this illness. We focus on specific testing considerations for repeat dose toxicity studies, including combination toxicity assessments, since new drug treatment regimens typically foresee short treatment durations to improve compliance (i.e., 1 day) with combinations of compounds to improve efficacy and limit potential resistance. Due to the target population, the timing of reproductive, developmental, and juvenile toxicity studies may be earlier than general testing roadmaps for other small molecule drugs.In conclusion, key recommendations presented should enable a more effective and efficient development path whilst protecting clinical trial participants and patients.

Harnessing the Power of Curcumin: Evaluating Its Efficacy as an Antileishmanial Agent in the Fight Against Leishmaniasis

Over 100 countries are impacted by the dangerous parasite disease leishmaniasis, which is categorized as a neglected tropical disease (NTD). Protozoan parasites use sandflies and other vectors to spread the disease. Herbal compounds like turmeric curcumin have demonstrated promise in the treatment of parasitic illnesses despite their limited bioavailability and water solubility. From small, self-healing skin lesions to serious intestinal infections, leishmaniasis can cause a wide range of illnesses in humans. Since there isn’t a gold standard test for one type, mucocutaneous leishmaniasis (MCL), it can be difficult to diagnose. Treatments for MCL can be quite harmful, which emphasizes how crucial a correct diagnosis. This study aims to assess the diagnostic performance of PCR (polymerase chain reaction) for MCL and to compare the findings with the authors’ most recent clinical research findings.

Enterobius vermicularis (pinworm) infestation mimicking acute appendicitis in two children from Palestine: a case report

BackgroundPinworm, Enterobius vermicularis, is a common parasitic illness, particularly among children in Palestine. On rare occasions, it can cause serious consequences such as acute appendicitis, which can still present a challenging diagnosis especially in children.Case presentationThis report describes two cases (a 9 year old male and a 11 year old female, both Palestinian children from Hebron district) of acute appendicitis referred to Queen Alia Hebron Governmental Hospital in Palestine. The clinical examination revealed a lower abdominal pain, and they were diagnosed with appendicitis. The pathological examination after appendectomy showed the presence of eggs and pinworms. Anthelminthic medication was provided, and they were followed up for 6 weeks after the operation without any incidents.ConclusionThese cases highlight the importance of considering Enterobius vermicularis infestation in children with abdominal pain, as the parasites can mimic appendicitis. Prompt recognition and cautious laparoscopic appendectomy are crucial to prevent unnecessary surgery and peritoneal contamination, with the infestation being easily treatable with anthelmintic medication.

Hydatid Cyst in Buffalo of Mandla District of Madhya Pradesh

Hydatidosis is the most prevalent parasitic illness of zoonotic importance affecting both humen and animals. This condition is triggered by the larval metacestode of the dog tapeworm, which primarily affects the health and lifestyles of subsistence farming communities in underdeveloped countries. Compared with other visceral organs, hydatid cysts are most likely to develop in the liver and lungs. Depending on its developmental stage, it can play a mechanical or toxic role in the hepatobiliary system. Owing to decreased output and the condemnation of visceral organs and carcasses, large-sized hydatid cysts cause significant economic loss. Hydatidosis is the most prevalent zoonotic parasite illness in both humen and animals. In the Mandla area of Madhya Pradesh, hydatid cysts have been found in buffalo carcasses that are 8-10 years old. One male and five female buffaloes that had previously died suddenly had postmortem lesions that revealed multiple hydatid cysts in the liver and abdominal cavities. Cysts that contained clear, watery, or serous fluid were varying in size. Some viscous fluid or caseous substances were present in hard cysts. The lungs and the liver were swollen and clogged. This explains the current state of the disease in the region and, consequently, the necessity for increasing focus on disease control and prevention.

An Overview of Parasitic Disease: Dracunculiasis

Dracunculiasis, also known as guinea worm disease, is a parasitic illness that only affects isolated, rural communities without access to clean drinking water in thirteen sub-Saharan African countries. The WHO has identified it as one of the next diseases to be eradicated. Water contaminated with copepods, or water fleas, carrying Dracunculiasis medinensis larvae is the vector of guinea worm disease transmission. A year after humans consume contaminated water, an adult female worm emerges, usually from a lower extremity, causing excruciating ulcers that can cause severe mobility impairment for several weeks. This illness strikes during the height of agricultural activity every year. A village's economically productive population is typically impacted in large numbers at the same time, which lowers agricultural output and causes economic difficulties. Since there is no known cure or vaccine for guinea worm disease, prevention is the key to its eradication. Globally, guinea worm disease cases have decreased by 98% since 1986, mostly due to community-based initiatives. Through these initiatives, the community has been taught how to filter water to get rid of parasites and how to stop people who have ulcers from contaminating drinking water sources. Sustained high-level political, financial, and community support will be necessary for complete eradication. By using an ayurvedic treatment approach, dracunculiasis can also be prevented. Ayurvedic medicine uses a few medicinal plants that exhibit antiparasitic activity against dracunculiasis, including Neem, Turmeric, Garlic, Amala, Baheda, Harde, Vidanga, Gokshura, Rasna, Bilva root, and Bala root.

Soil-transmitted helminths and associated factors among pregnant women in Doreni district, Oromia region, Ethiopia: a cross-sectional study

BackgroundSoil-transmitted helminthiasis (STH) refers to a set of parasitic illnesses caused by nematode worms and spread to people through faeces-contaminated soil. It is highly prevalent in low- and middle-income countries due to a lack of environmental sanitation and personal hygiene. Pregnant women are among the risk groups for infection by soil-transmitted helminths. Former studies of the disease burden among pregnant women in Ethiopia didn’t indicate the intensity of parasitic infection. The aim of this study was to assess the prevalence and associated factors of soil-transmitted helminths among pregnant women.MethodsAn institution-based cross-sectional study was conducted among 416 randomly selected pregnant women. The data were collected using a structured interview-administered questionnaire and a laboratory test. The Kato-Katz technique was used to diagnose soil-transmitted helminthiasis and determine the intensity of the infection. The collected data were entered into Epi-Data version 4.6 and exported to SPSS version 25 for analysis. Multivariate logistic regression analysis was used to identify independent predictors of soil-transmitted helminths at a p-value < 0.05.ResultsThe overall prevalence of soil-transmitted helminths among pregnant women was 30%. (95%, CI: 26-34%). Living in a rural area (AOR = 3.35; 95% CI = 1.83–6.13), drinking from an unprotected water source (AOR = 2.52; 95% CI = 1.45–4.37), not washing one’s hand after the toilet (AOR = 2.75; 95% CI = 1.55–4.88), lacking health information (AOR = 1.70; 95% CI = 1.01–2.85), working as a daily labourer (AOR = 2.88; 95% CI = 1.01–8.20), and walking barefoot (AOR = 4.00; 95% CI = 2.29–7.00) were significantly associated with the presence of soil-transmitted helminths among pregnant women.ConclusionThe prevalence of STH was significantly moderate in the study area, where pregnant women were mostly affected by ascariasis and hookworms. Living in a rural area, being a daily labourer, walking barefoot, not washing hands after the toilet, drinking from an unprotected water source, and lacking health information were the determining factors. Interventions including health education, the expansion of pure drinking water sources, the promotion of personal hygiene, and the wearing of shoes are recommended to reduce the burden of soil-transmitted helminths in the study area.

Structure - Guided design of arctigenin derivatives against infections of Gyrodactylus kobayashii

Gyrodactylosis is a widespread parasitic illness that affects fish in both farming and natural environments. The current chemotherapy approaches are not always satisfactory, so the development of new anthelmintic agents is necessary. Arctigenin, a natural lignan found in various plants, possesses a multitude of biological activities. Previous research has demonstrated that arctigenin targets the protein myosin in Gyrodactylus kobayashii. Building upon this interaction, we designed 31 derivatives of arctigenin using Discovery Studio. The ester derivatives exhibited significant anthelmintic activity, with C-21 displaying the highest activity, boasting an EC50 of 0.4 mg/L. Furthermore, we conducted an investigation into the mechanism by which C-21 affects the parasite and discovered that it disrupts the muscular structure of the parasite's body surface, thus inhibiting its energy production and myosin expression, ultimately leading to the parasite's demise. In conclusion, C-21 exhibits promising potential as an agent for controlling Gyrodactylus kobayashii infection.

Machine learning approach for predicting cardiovascular disease in Bangladesh: evidence from a cross-sectional study in 2023

BackgroundCardiovascular disorders (CVDs) are the leading cause of death worldwide. Lower- and middle-income countries (LMICs), such as Bangladesh, are also affected by several types of CVDs, such as heart failure and stroke. The leading cause of death in Bangladesh has recently switched from severe infections and parasitic illnesses to CVDs.Materials and methodsThe study dataset comprised a random sample of 391 CVD patients' medical records collected between August 2022 and April 2023 using simple random sampling. Moreover, 260 data points were collected from individuals with no CVD problems for comparison purposes. Crosstabs and chi-square tests were used to determine the association between CVD and the explanatory variables. Logistic regression, Naïve Bayes classifier, Decision Tree, AdaBoost classifier, Random Forest, Bagging Tree, and Ensemble learning classifiers were used to predict CVD. The performance evaluations encompassed accuracy, sensitivity, specificity, and area under the receiver operator characteristic (AU-ROC) curve.ResultsRandom Forest had the highest precision among the five techniques considered. The precision rates for the mentioned classifiers are as follows: Logistic Regression (93.67%), Naïve Bayes (94.87%), Decision Tree (96.1%), AdaBoost (94.94%), Random Forest (96.15%), and Bagging Tree (94.87%). The Random Forest classifier maintains the highest balance between correct and incorrect predictions. With 98.04% accuracy, the Random Forest classifier achieved the best precision (96.15%), robust recall (100%), and high F1 score (97.7%). In contrast, the Logistic Regression model achieved the lowest accuracy of 95.42%. Remarkably, the Random Forest classifier achieved the highest AUC value (0.989).ConclusionThis research mainly focused on identifying factors that are critical in impacting patients with CVD and predicting CVD risk. It is strongly advised that the Random Forest technique be implemented in a system for predicting cardiac diseases. This research may change clinical practice by providing doctors with a new instrument to determine a patient’s CVD prognosis.

Machine learning for predicting Chagas disease infection in rural areas of Brazil.

Chagas disease is a severe parasitic illness that is prevalent in Latin America and often goes unaddressed. Early detection and treatment are critical in preventing the progression of the illness and its associated life-threatening complications. In recent years, machine learning algorithms have emerged as powerful tools for disease prediction and diagnosis. In this study, we developed machine learning algorithms to predict the risk of Chagas disease based on five general factors: age, gender, history of living in a mud or wooden house, history of being bitten by a triatomine bug, and family history of Chagas disease. We analyzed data from the Retrovirus Epidemiology Donor Study (REDS) to train five popular machine learning algorithms. The sample comprised 2,006 patients, divided into 75% for training and 25% for testing algorithm performance. We evaluated the model performance using precision, recall, and AUC-ROC metrics. The Adaboost algorithm yielded an AUC-ROC of 0.772, a precision of 0.199, and a recall of 0.612. We simulated the decision boundary using various thresholds and observed that in this dataset a threshold of 0.45 resulted in a 100% recall. This finding suggests that employing such a threshold could potentially save 22.5% of the cost associated with mass testing of Chagas disease. Our findings highlight the potential of applying machine learning to improve the sensitivity and effectiveness of Chagas disease diagnosis and prevention. Furthermore, we emphasize the importance of integrating socio-demographic and environmental factors into neglected disease prediction models to enhance their performance.

Visceral Leishmaniasis (VL) Clinical Presentation, Laboratory Findings, Treatment Options and Outcome.

Black disease, also known as visceral leishmaniasis (VL), is a parasitic illness caused by various Leishmania species. The risk of morbidity and mortality increases with delayed diagnosis and treatment. Early VL diagnosis and fast appropriate treatment are critical issues in endemic areas. This study was a retrospective cross-sectional study to investigate the diagnostic and therapeutic course of patients admitted with the diagnosis of VL in the Children's Medical Center (CMC) Hospital, Tehran, Iran. All cases of VL in patients under the age of 18 hospitalized between the years 2012 to 2022 were enrolled. Twenty-seven patients were enrolled with an average age of 28.13 months with the majority of females (51.8%). Common clinical signs were fever (96.2%) and splenomegaly (92.59%). However, lymphadenopathy was rare. The largest number of patients was from Tehran Province, followed by Ardabil, Khuzestan, Gilan, and Alborz provinces. The most common hematological abnormalities were anemia (85.1%) and thrombocytopenia (44.4%). In accordance with the treatment strategy, liposomal amphotericin B and amphotericin B deoxycholate were given to 11 and 5 patients, respectively. Eleven of them received glucantime. The average length of hospitalization for liposomal amphotericin B was 15.36 ± 12.49 days. In comparison with glucantime (18.38 ±10.26 days) and amphotericin B deoxycholate (20.20± 6.18 days), liposomal amphotericin B group hospitalization was shorter than others were. VL should be included in the differential diagnosis of any child who presents with fever, splenomegaly, and anemia. Concerning the treatment strategy in this study, liposomal amphotericin B had more efficiency and shorter hospitalization duration.

Ethnobotanical study of medicinal plants used by the people of Mosop, Nandi County in Kenya.

Background: Throughout the history, nature has provided mankind with most of their basic needs, which include food, shelter, medicine, clothes, flavours, scents as well as raw materials. Given that they are an integral part of cultural heritage, medicinal plants have played a significant role in human healthcare systems around the world. Investigating various biological resources for use as medicines requires ethnomedicinal studies. Methods: Data on utilization of ethnomedicinal plants from local healers in Kenya's Mosop Sub-County in Nandi County was documented through open-ended, semi-structured questionnaires. A number of quantitative indices, such as the Use Citation (UC), Informant Consensus Factor (ICF), Use Value (UV), Frequency of Citation (FoC) and Relative Frequency of Citation (RFC) were used to convey the potential medical benefits, vitality and variety of the ethnomedicine. Results: 102 informants provided information on 253 ethnomedicinal plant species, classified into 74 families. There were 249 native plant species identified, along with few exotic species: Senegalia senegal (L.) Britton, Persea americana Mill, Carica papaya L. and Solanum betaceum Cav. Of all recorded species, 32% and 27% were herbs and trees, respectively. Among plant parts, leaves were most frequently utilized (27%) and roots (26%), while decoctions (21%) were the most widely used formulations. The dominant family was Asteraceae, with 28 species, followed by Lamiaceae, with 19 species. The highest ICF value was 0.778 for a number of parasitic and infectious illnesses, including ringworms, athlete's foot rot, tetanus, typhoid, intestinal parasites, abscesses, malaria, and amoebiasis. The study's data validates the region's widespread use of traditional medicinal plant remedies. Conclusion: The current study will lay a foundation of knowledge for future research investigations. The abundance of knowledge regarding ethnomedicinal species and their medicinal applications will stimulate further phytochemical and pharmacological research, which could lead to the discovery of potentially significant pharmaceuticals.

Renal hydatid cyst - An exceptional presentation

The infectious parasitic illness echinococcosis, also known as hydatidosis, mostly arises from the larval forms of the tapeworm species Echinococcus granulosus. The prevalence of renal involvement in total hydatid disease is documented to be approximately 2-3%, a notably lower rate in comparison to the involvement observed in the liver and lungs. As a result of the lack of observable symptoms, the cyst remains asymptomatic and exhibits a slow progression over a period of time. The computed tomography (CT) scan is often regarded as a highly accurate diagnostic procedure owing to its capacity to precisely ascertain the size and location of the lesion, as well as its correlation and influence on surrounding tissue. The most effective therapeutic strategy entails implementing surgical procedures to extract a hydatid cyst involving the renal system.

Understanding the mechanisms underlying the disruption of the blood-brain barrier in parasitic infections.

Parasites have a significant impact on the neurological, cognitive, and mental well-being of humans, with a global population of over 1 billion individuals affected. The pathogenesis of central nervous system (CNS) injury in parasitic diseases remains limited, and prevention and control of parasitic CNS infections remain significant areas of research. Parasites, encompassing both unicellular and multicellular organisms, have intricate life cycles and possess the ability to infect a diverse range of hosts, including the human population. Parasitic illnesses that impact the central and peripheral nervous systems are a significant contributor to morbidity and mortality in low- to middle-income nations. The precise pathways through which neurotropic parasites infiltrate the CNS by crossing the blood-brain barrier (BBB) and cause neurological harm remain incompletely understood. Investigating brain infections caused by parasites is closely linked to studying neuroinflammation and cerebral impairment. The exact molecular and cellular mechanisms involved in this process remain incomplete, but understanding the exact mechanisms could provide insight into their pathogenesis and potentially reveal novel therapeutic targets. This review paper explores the underlying mechanisms involved in the development of neurological disorders caused by parasites, including parasite-derived elements, host immune responses, and modifications in tight junctions (TJs) proteins.

Updated Investigation on Blood-Borne Parasitic Illnesses and Tick Infestation in Daulatpur, Khulna, Bangladesh: Incidence and Determinants

Updated Investigation on Blood-Borne Parasitic Illnesses and Tick Infestation in Daulatpur, Khulna, Bangladesh: Incidence and Determinants

Microbial and Parasitic Infection in Fish

Major concerns in the fisheries business are parasitic fish and bacterial infections. When it comes to aquatic life microbes are a regular occurrence. The majority of microbes are naturally occurring in water. Because of poor environmental conditions, limited nutrition, and inefficient fish husbandry practices, they only cause diseases. Some microbial infections occur in fresh water fish (Pseudomonas infection, Acinetobacter infections, bacterial gill diseases), in marine water (Vibriosis, Tenacibaculosis, streptococcal infections, bacterial skin diseases) and in brackish water (Red sea bream iridoviral disease (RSID, Lymphocystis disease (LCD). All these infections affect fish differently. Parasitic illnesses can cause epidemics in fish that are overcrowded or under other unnatural situations. A large number of fish species have been severely impacted by parasite and bacterial species. Some parasitic infections of fresh water are Renal Sphaerosporosis, due to leeches and nematodes, isopods and Gill Monogeneans; in amarine water are Microsporidiosis, Cryptocaryonosis and Brooklynelliosis and in brackish water are Diplostomum spathaceum metacercarial infections. Some preventive actions are done to control this problem. In this review there is a complete detail about parasite and bacterial infections and how they can be prevented.