In the natural ecosystems, parasitic Hymenoptera composes the most significant group of biocontrol agents. DNA barcode (658 bp sequence from the 5′-end of cytochromeoxidase I) analysis of hymenopterans collected in a Malaise trap in Chittagong university campus was performed to analyze the diversity of parasitic wasps. In the present study a total of 3,468 sequences were generated that represented 31 species, 83 genera and 22 families from seven superfamilies of Hymenoptera. Among them 25 species namely <i>Aphanogmus fijiensis</i> Ferriere, <i>Telenomus remus</i> Nixon, <i>Ganaspis xanthopoda</i> Ashmead, <i>Encarsia sophia</i> Girault & Dodd, <i>Copidosoma floridanum</i> Ashmead, <i>C. thebe</i> Walker, <i>Ceranisus menes</i> Walker, <i>Hemiptarsenus varicornis</i> Girault, <i>Eupelmus martellii</i> Masi, <i>Trichogramma achaeae</i> Nagaraja and Nagarkatti, <i>Trichogrammatoidea bactrae</i> Nagaraja, <i>Binodoxys acalephae</i> Marshall, <i>B. communis</i> Gahan, <i>Aspidobracon noyesi</i> van Achterberg, <i>Bracon crassicornis</i> Thomson, <i>Cardiochiles fuscipennis</i> Szepligeti, <i>Apanteles boaris</i> Walker, <i>Glyptapanteles creatonoti</i> Viereck, <i>Phaedrotoma depressa</i> Li & van Achterberg, <i>Psyttalia fletcheri</i> Silvestri, <i>Aleiodes malichi</i> Quicke & Butcher, <i>A. prillae</i> Quicke & Butcher, <i>A. sutthisani</i> Quicke & Butcher, <i>Diplazon orientalis</i> Cameron, <i>Exochus pictus</i> Holmgren, 58 genera, 12 subfamilies and 7 named families specifically Megaspilidae, Figitidae, Eupelmidae, Ormyridae, Perilampidae, Torymidae and Trigonalyidae are the first reports from Bangladesh. The results of this study will be resulted to determining of parasitic hymenopteran fauna in Bangladesh.