The study aims to evaluate the reliability of the novel dentin thickness model based on root parameters when applied to panoramic radiography images of mandibular first molar in the southern Turkish subpopulation. A total of 946 panoramic images were analyzed for the study. The samples were allocated into four age groups: Group 1(between 10 and 18), Group 2(between 19 and 30), Group 3(between 31 and 50), Group 4(between 51 and 78). The dentin thickness model measurements consisted of root dentin thickness of the mesial aspect of the distal root and distal canal width. Regression, intraclass correlation coefficient, and Durbin-Watson analyses were used for statistics. A medium correlation was detected in the dentin thickness model (R = 0.398). Root dentin thickness and canal width had a significant effect (p < 0.05), but gender did not (p > 0.05). Group 3 showed the highest accuracy (p < 0.001). Group 4 presented the lowest correlation with chronological age compared to other age groups (p < 0.001). The dentin thickness model showed a promising result for the dental age estimation with a medium correlation. The dentin thickness model included root measurements of the mesial aspect of the mandibular first molar without any crown parameters in the southern Turkish subpopulation. Gender did not affect the dentin thickness model.