Pancreas Of Diabetic Rats Research Articles

Semi-modified okara whey diet increased insulin secretion in diabetic rats fed a basal or high fat diet.

Lifestyle and diet preferences are primarily responsible for developing type 2 diabetes. In this study, okara was manufactured into okara whey crackers (OWC) to investigate its dietary role in controlling diabetes in streptozotocin-diabetic rats with and without a high-fat diet. Forty-eight rats were divided into eight groups. G1-G4 were nondiabetic and fed a basal diet, a basal diet with 30% crackers, high fat diet, and a high-fat diet with 30% crackers, respectively. G5-G8 were diabetic groups that received similar diets as previous groups. Blood glucose, liver function, lipid pattern, pancreas and liver histopathology, and insulin immunohistochemistry were performed. OWC improved measured parameters and histopathology of the liver and pancreas in diabetic rats. The area % of positive insulin cells was increased in G6 (5.20%) and G8 rats (2.83%) fed OWC compared to diabetic rats (1.17%). In conclusion, the use of 30% OWC in a semi-modified diet has controlled the hyperglycemia and hyperlipidemia associated with diabetes.

Modulation of the antioxidant defense system in liver, kidney, and pancreas tissues of alloxan-induced diabetic rats by camphor.

Excess production of reactive oxygen species and the loss of antioxidant efficiency systems play an important role in the development of diabetes. Thus, using antioxidant compounds is an important strategy to reduce these complications. This study aimed to investigate the possible effect of camphor on the serum biochemical parameters and oxidative parameters in the pancreas, liver, and kidney tissues of alloxan-induced diabetic rats. In this study, 40 male Wistar rats were divided into five groups, including normal group, diabetic group, the diabetic group treated with glibenclamide, the diabetic group treated with 30mg/kg camphor, and the diabetic group receiving the camphor solvent. Intragastric administration of camphor and glibenclamide, as the control drug, to diabetic rats for 21days lowered their blood glucose, total cholesterol, triglyceride, and LDL-cholesterol, while the blood high-density lipoprotein -cholesterol level was increased. In addition, our results indicated that treatment of diabetic rats with camphor increased the activity of glutathione peroxidase, catalase, and superoxide dismutase enzymes as well as reduced glutathione content in the liver, pancreas, and kidney tissues as compared to the diabetic rats. Based on our data, it can be concluded that camphor has a hypoglycemic activity, and this effect may be attributed, in part, to its antioxidant ability. PRACTICAL APPLICATIONS: Camphor is a terpenoid natural compound derived from the wood present in the camphor laurel's stem and roots (Cinnamomum camphora L.) trees. The synthetic form of camphor is currently being produced for medical, health, and industrial applications. In addition, this compound is present in Rosmarinus officinalis, Cinnamomum zeylanicum, Salvia officinalis, Artemisia Annua, and Ocimum basilicum. Numerous researches have shown its beneficial effects on various diseases. In this study, it has been shown that camphor possesses antidiabetic effects in alloxan-induced diabetic rats. Treatment of diabetic rats with camphor increased the antioxidant capacity and reduces the oxidative stress markers in the liver, pancreas, and kidney tissues as compared to the diabetic rats. Given the favorable effect of camphor on some oxidative parameters in diabetic rats in our study, its antihyperglycemic property is probably due to its antioxidant effects.

Diabetic complications and oxidative stress: The role of phenolic‐rich extracts of saw palmetto and date palm seeds

Recently, we reported that the date palm seed (DP) and saw palmetto seed (SP) extracts possessed a great amount of phenolic contents with potent antioxidant, antimicrobial, and anti-inflammatory activities. Therefore, this study aimed to assess the role of DP and SP phenolic-rich extracts in modulating diabetic complications and oxidative stress in the STZ- diabetic rat. DP and SP extracts significantly inhibited both microbial and pancreatic α-amylases. The STZ-induced diabetic rat groups treated with DP and SP extracts exhibited reversed hyperglycemia (40% and 54%, p<.001-.01) and body weight (70%, p<.001) alteration close to normal. Moreover, DP and SP extracts modulated serious damages in the structures of the pancreas, kidney, and liver of diabetic rats. DP and SP extracts improved the decline of the activities of antioxidant enzymes: Catalase, glutathione-S-transferase, glutathione reductase, and glutathione peroxidase in liver, kidney, and pancreas of the diabetic rats. PRACTICAL APPLICATIONS: Generally, date seed is a rich source of dietary fibers, polyphenols, and antioxidants and used in foods and pharmaceuticals. Our study reported that date palm seed (DP) and saw palmetto seed (SP) phenolic-rich extracts attenuated diabetes and its complications, probably tissue regeneration and normalizing the oxidative stress in the STZ-induced diabetic rat.

Antihyperglycemic and antihyperlipidemic activities of wild musk melon (Cucumis melo var. agrestis) in streptozotocin-nicotinamide induced diabetic rats

ObjectiveWild musk melon (Cucumis melo var. agrestis, CMA) is one of the edible plants form Tamil Nadu. Traditionally, this plant was used as diabetic diet (leaves of CMA with Momordica charantia leaves), but there is no scientific report on antidiabetic action of this plant material. Hence, the current research work was designed to evaluate the antihyperglycemic and antihyperlipidemic effect of hydroalcoholic extract of CMA leaves (HALEC) in streptozotocin (STZ)-nicotinamide (NIC)-induced diabetic rats. MethodsDiabetes was induced by administration of STZ (60 mg/kg, i.p.) after 15 min of NIC (120 mg/kg i.p.) administration. The diabetic rats were treated with HALEC (300 and 600 mg/kg, p.o., respectively) for 21 d. ResultsAfter the management with HALEC, blood glucose, HbA1c levels, total cholesterol, LDL cholesterol, triglycerides levels, glycogen phosphorylase and glucose-6-phosphatase levels were significantly diminished in diabetic rats. However, haemoglobin level, HDL cholesterol, liver glycogen, total protein, hexokinase, glucose-6-phosphate dehydrogenase levels were significantly increased in HALEC treated diabetic rats. The histopathological studies of the pancreas in HALEC-treated diabetic rats showed almost normal appearance. L6 cell line study revealed the increased glucose uptake activity of HALEC. High performance thin layer chromatography (HPTLC) analysis confirms the presence of active principles such as rutin, gallic acid and quercetin in HALEC. ConclusionThe results indicated that HALEC possess significant antihyperglycemic and antihyperlipidemic activity in STZ-NIC-induced type II diabetic rats with protective effect. This research work will be useful for the isolation of active principles and development of herbal formulation in phytopharmaceuticals.

Effect of Curculigo pilosa supplemented diet on blood sugar, lipid metabolism, hepatic oxidative stress and carbohydrate metabolism enzymes in streptozotocin-induced diabetic rats.

Diabetes mellitus (DM) has continued to raise concern globally and Curculigo pilosa (CP) is used for its treatment and management in folkloric medicine. In this study, the in vitro antioxidant abilities of CP and the effects of CP-supplemented diets on blood sugar, lipid metabolism, oxidative stress and key carbohydrate metabolizing enzymes in streptozotocin (STZ)-induced diabetic rats were investigated. Polyphenol contents (total phenolic and total flavonoid) and antioxidant ability of different extracts of CP were determined in vitro. Diabetes mellitus were stimulated in healthy rats by single intraperitoneal administration of 50mg/kg streptozotocin and it was confirmed by elevated blood glucose level after 3days. Thirty six rats were distributed into six groups of six rats each and diabetic rats were fed with 5 and 10% CP-supplemented diet for 21days. Thereafter, the effects of the dietary regimen were evaluated on blood glucose, body weight, hepatic carbohydrate metabolizing enzymes, lipid profile, oxidative stress markers, serum markers of hepatic and renal damages and histopathology studies. Different extracts of CP contained polyphenol contents and exhibited antioxidant properties in different models used. Diabetic rats showed elevated level of blood glucose and body weight loss. Treatment of diabetic rats with CP-supplemented diet significantly (p < 0.05) lowered the blood glucose and improved body weight loss. Also, the treatment with the CP-supplemented diet significantly (p < 0.05) enhanced the activities of hepatic glycolytic (hexokinase and glucose-6-dehydrogenase) and lowered the gluconeogenic (fructose 1, 6 biphosphatase and glucose-6-phosphatase) enzymes in diabetic rats. The lipid profile, oxidative stress markers and serum markers of hepatic and renal damages were significantly (p < 0.05) restored to near normalcy in the diabetic rats. Histopathological slides also showed improvements in pancreas and hepatic tissues of diabetic rats treated with CP-supplemented diet. Data obtained in this study suggested that CP-supplemented diet could be used as dietary regimen in the management of DM.

The Histological Changes in Endocrine Part of the Pancreas of Diabetic Rats Treated with Rosa Damascena Extract

The Histological Changes in Endocrine Part of the Pancreas of Diabetic Rats Treated with Rosa Damascena Extract

Anethum graveolens L‎. Alleviates Sperm ‎Damage by Limiting Oxidative Stress ‎and Insulin ‎Resistance ‎in ‎Diabetic Rats ‎

Background: It has been reported that diabetes is associated with sperm ‎damage and infertility. Objective: The purpose of this experiment was to survey the effect of Anethum graveolens L. (Dill) powder on sperm profiles, oxidative stress, insulin resistance, and histological changes in male diabetic rats. Methods: Male rats were randomly divided into 6 groups (n=7); group 1: normal rats, 2: normal rats + 100mg/kg Dill, 3: normal rats + 300mg/kg Dill, 4: diabetic rats, 5: diabetic rats + 100mg/kg Dill, and 6: diabetic rats + 300mg/kg Dill. After 2 months of treatments, the sperm profile, anti-oxidant activity, superoxide dismutase (SOD) activity, and malondialdehyde were measured. The histopathology of testis was evaluated. Hormonal changes and tumor necrosis factor-α (TNF-α) levels were measured by ELISA. Results: Total anti-oxidant and SOD activity in diabetic rats significantly decreased, while MDA concentration was significantly increased in the testis and pancreas of diabetic rats compared with control. However, the use of Dill significantly normalized these profiles. The treatment of diabetic rats with Dill changed the sperm parameters. The levels of testosterone, FSH, and LH in diabetic rats were significantly reduced, but the treatment with Dill did not alter the level of these hormones. Dill also significantly normalized testis morphological changes, insulin resistance, and inflammation. Conclusion: The use of Dill normalized oxidative stress, inflammation, and insulin resistance in diabetic rats that correlated with sperm profile and testis histological changes. The treatment of diabetic rat models with Dill did not show harmful effects on sperm profiles.

Antidiabetic and antidyslipidemic activities of hexane/ethyl acetate/methanol fractions of Paspalum scrobiculatum linn. grains in high-fat diet and streptozotocin-induced diabetic rats

Background: Dietary habits and sedentary lifestyle are the major causes of increasing obesity, which in turn causes diabetes and cardiovascular problems. With this alarming increase, there is a need for alternative therapies to treat diabetes and hyperlipidemia. Objectives: To evaluate antidiabetic and antidyslipidemic effects of fractions of Paspalum scrobiculatum grains in rats. Materials and Methods: Different doses of Hexane: Ethyl acetate fraction of P. scrobiculatum (HEPS) and Ethyl acetate: Methanol fraction of P. scrobiculatum (EMPS) fractions of P. scrobiculatum were administered to the high-fat diet (HFD) and streptozotocin (STZ) (35 mg/kg)-induced diabetic rats with glibenclamide at 20 mg/kg body weight (b.w) as a standard reference. Biochemical and histopathological changes were assessed after 5 weeks of treatment. Results: Daily oral treatment with HEPS and EMPS each at 100 and 200 mg/kg b.w for 5 weeks was found to be significant in the reduction of plasma glucose levels, HbA1c, increased plasma insulin levels, and normalized lipid profile and liver function parameters in HFD and STZ-induced diabetic rats. In addition, degenerative histopathological changes seen in liver, kidney, and pancreas of diabetic rats were found to be normalized in HEPS and EMPS as comparable to that of glibenclamide-treated rats. Conclusion: The obtained results suggest that HEPS and EMPS fractions of P. scrobiculatum grains possess promising antihyperglycemic and antidyslipidemic potential without apparent toxic effects, which may prove the claimed use of the plant in treatment of diabetes and dyslipidemia.

TOTAL FLAVONOID DAN EFEKTIVITAS EKSTRAK ETANOL BIJI KELOR (Moringa oleifera L) ASAL KOTA PALU SULAWESI TENGAH TERHADAP HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI STREPTOZOTOCIN

This study aims to determine the content of flavonoid and the effect of ethanol extract of moringa seeds on the regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes. This study method used has total flavonoid equivalent quercetin by spectrophotometry uv-vis and to regeneration of pancreatic β cells in male white rats used 30 test animals,namely male white rats divided into 6 groups, each group consisted of 5 male white rats with details of group I as normal control, Group II as negative control given 0.5% Na-CMC suspension, Group III as positive control given glibenclamide suspension and in Groups IV, V, and VI were given with each dose of 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BB. Histopathological damage picture of the pancreas was observed by staining HE using a 400x magnification olympus Cx21 microscope. The results showed that the ethanol extract of moringa seeds contained secondary metabolites, namely flavonoids, alkaloids, saponins and tannins. The results showed has total flavonoid equivalent quercetin of moringa seeds is 1,26% and regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes of Moringa seed ethanol extract at a dose of 400 mg/kg BB can have an effect on the regeneration of β cells in the pancreas of white diabetic male rats.&#x0D;

Hypoglycemic mechanism of polysaccharide from Cyclocarya paliurus leaves in type 2 diabetic rats by gut microbiota and host metabolism alteration.

Diabetes mellitus is a serious threat to human health. Cyclocarya paliurus (Batal.) Iljinskaja (C.paliurus) is one of the traditional herbal medicine and food in China for treating type 2 diabetes, and the C. paliurus polysaccharides (CP) were found to be one of its major functional constituents. This research aimed at investigating the hypoglycemic mechanism for CP. It was found that CP markedly attenuated the symptoms of diabetes, and inhibited the protein expression of Bax, improved the expression of Bcl-2 in pancreas of diabetic rats, normalized hormones secretion and controlled the inflammation which contributed to the regeneration of pancreatic β-cell and insulin resistance. CP treatment increased the beneficial bacteria genus Ruminococcaceae UCG-005 which was reported to be a key genus for protecting against diabetes, and the fecal short-chain fatty acids levels were elevated. Uric metabolites analysis showed that CP treatment helped to protect with the diabetes by seven significantly improved pathways closely with the nutrition metabolism (amino acids and purine) and energy metabolism (TCA cycle), which could help to build up the intestinal epithelial cell defense for the inflammation associated with the diabetes. Our study highlights the specific mechanism of prebiotics to attenuate diabetes through multi-path of gut microbiota and host metabolism.

Influence of Fortification of Biscuits with Hyphaene thebaica Flour on Quality Attributes, Biochemical Parameters and Histological Examination of Pancreas in Diabetic Rats

Influence of Fortification of Biscuits with Hyphaene thebaica Flour on Quality Attributes, Biochemical Parameters and Histological Examination of Pancreas in Diabetic Rats

Transplantation of human dental pulp stem cells in streptozotocin-induced diabetic rats.

Type 1 diabetes mellitus (T1DM) is a chronic metabolic disease caused by the destruction of pancreatic β-cells. Human dental pulp stem cells represent a promising source for cell-based therapies, owing to their easy, minimally invasive surgical access, and high proliferative capacity. It was reported that human dental pulp stem cells can differentiate into a pancreatic cell lineage in vitro; however, few studies have investigated their effects on diabetes. Our study aimed to investigate the therapeutic potential of intravenous and intrapancreatic transplantation of human dental pulp stem cells in a rat model of streptozotocin-induced type 1 diabetes. Forty Sprague Dawley male rats were randomly categorized into four groups: control, diabetic (STZ), intravenous treatment group (IV), and intrapancreatic treatment group (IP). Human dental pulp stem cells (1 × 106cells) or vehicle were injected into the pancreas or tail vein 7days after streptozotocin injection. Fasting blood glucose levels were monitored weekly. Glucose tolerance test, rat and human serum insulin and C-peptide, pancreas histology, and caspase-3, vascular endothelial growth factor, and Ki67 expression in pancreatic tissues were assessed 28days post-transplantation. We found that both IV and IP transplantation of human dental pulp stem cells reduced blood glucose and increased levels of rat and human serum insulin and C-peptide. The cells engrafted and survived in the streptozotocin-injured pancreas. Islet-like clusters and scattered human dental pulp stem cells expressing insulin were observed in the pancreas of diabetic rats with some difference in the distribution pattern between the two injection routes. RT-PCR analyses revealed the expression of the human-specific pancreatic β-cell genes neurogenin 3 (NGN3), paired box 4 (PAX4), glucose transporter2 (GLUT2), and insulin in the pancreatic tissues of both the IP and IV groups. In addition, the transplanted cells downregulated the expression of caspase-3 and upregulated the expression of vascular endothelial growth factor and Ki67, suggesting that the injected cells exerted pro-angiogenetic and antiapoptotic effects, and promoted endogenous β-cell replication. Our study is the first to show that human dental pulp stem cells can migrate and survive within streptozotocin-injured pancreas, and induce antidiabetic effects through the differentiation and replacement of lost β-cells and paracrine-mediated pancreatic regeneration. Thus, human dental pulp stem cells may have therapeutic potential to treat patients with long term T1DM.

Effects of Coenzyme Q10 on Some Blood Antioxidant System Parameters and Histological Changes in the Pancreas and Aorta of Streptozotocin-induced Diabetic Rats

Bu calismanin amaci diyabet olusturulan ratlarda koenzim Q10’un antioksidan parametreleri uzerine etkilerini degerlendirmektir. Calismada 38 yetiskin, erkek Wistar Abino rat (250-300 gr) kullanildi. Grup I’deki hayvanlara herhangi bir uygulama yapilmadi. Grup II’deki hayvanlara gunde 0.3 ml misir yagi dort hafta boyunca intraperitoneal olarak uygulandi. Grup III’deki hayvanlara gunde 0.3 ml misir yaginda cozdurulen 10 mg/kg CoQ10 dort hafta boyunca intraperitoneal olarak uygulandi. Grup IV’deki hayvanlarda, 40 mg/kg streptozotosinin gunde tek doz olmak uzere iki subkutan enjeksiyonu ile diyabet olusturuldu. Grup V’deki hayvanlarda ayni protokolle diyabet olusturuldu ve daha sonra bu hayvanlara dort hafta boyunca 0.3 ml misir yaginda cozdurulen 10 mg/kg CoQ10 intraperitoneal olarak uygulandi. Kan orneklerinde GSH, TBARS, SOD, NO seviyeleri ile GPx, CAT aktiviteleri belirlendi. Pankreas ve aorta doku ornekleri histolojik ve immunohistokimyasal yontemler kullanilarak incelendi. Diyabetik grupta plazma SOD, GPx, CAT ve GSH seviyeleri control grubuna gore onemli oranda dusuktu. Diyabetik ratlara CoQ10 uygulamasi ile bu parametreler diyabetik gruba gore onemli oranda artti. Diyabetle birlikte artan plazma TBARS seviyesi, CoQ10 uygulamasiyla azaldi. Calismanin histolojik bulgulari CoQ10 uygulamasi sonucunda enzim duzeylerindeki degisiklikleri desteklemektedir. Sonuc olarak, diyabetik ratlara CoQ10 uygulamasi diyabetin neden oldugu bazi olumsuz degisiklikler uzerine faydali etkilere sahip olabilir.

Negligible Protection of Pancreas by Topical Curcumin in Streptozotocin Induced Diabetic Wounded Rats

Damage or non-functioning of beta cells of Langerhans is lets of pancreas causes Type I diabetes. Curcumin, a bioflavonoid, have shown some tissue regenerative properties. It has shown a very good wound healing actions in diabetic animals after topical applications. In view of this, we planned to study the regenerative action of curcumin on damaged pancreas of diabetic rats after its topical applications on wounds. Damage of pancreas and induction of Type I diabetes in rats was achieved by single intraperitoneal injection of streptozotocin (60mg/kg b.wt.). Thereafter, open excisional wounds were created and wounded rats were divided into three groups viz. i) healthy control (non-diabetic), ii) Diabetic control and iii) Diabetic-treated. Pluronic F-127 gel (25%) was applied topically on wounds of group I and II once daily for 19 days. Curcumin (0.3%) in gel was topically applied similarly on group III. Average body weight gain was less and blood glucose levels were high in group II and III. There were also higher levels of MDA in group II and III. Histopathologically, atrophy and loss of intactness of beta cells of langerhans is lets in the pancreas were evident in group II and III, as compared to group I. In conclusion, topical applications of curcumin on wounds of diabetic rats did not able to prevent the damage of beta cells of the pancreas produced by single intraperitoneal injection of streptozotocin (60mg/kg b.wt.). In future, the other higher or lower concentrations of curcumin and their nano-formulations may be tried to evaluate the protective actions.

The Beneficial Effects of Actinomycetales Immune Modulators in the Pancreas of Diabetic Rats.

Purpose: Type 1 diabetes mellitus (T1DM) has dramatically increased in recent years, especially in young people, and limits the life quality of the patients involved. Thus, many researchers are performing extensive studies to find alternative treatments for DM.Methods: Here, we evaluated the improvement effects of the heat-killed Actinomycetales species, including Gordonia bronchialis, and Tsukamurella inchonensis in streptozotocin (STZ)- diabetic rats by biochemical, immunological, and histopathological examinations.Results: The present findings exhibited a dramatic and progressive alteration in the serum levels of interleukin-6 (IL-6), IL-10 and tumor necrosis factor-α (TNF-α) in the diabetic group, which were related to the blood glucose and insulin levels, oxidative stress defense (evaluated by TAC and MDA activities), and the pancreas biochemical indicators (such as amylase and lipase). More importantly, the present results were consistent with the histopathological findings, which included cellular degeneration, vascular congestion, hemorrhage, focal necrosis associated with mononuclear cell infiltration. Interestingly, all of the diabetic changes in the blood serum and tissues improved remarkably in the treated groups by Actinomycetales species.Conclusion: Surprisingly, most of the current diabetic complications effectively attenuated after oral administration of both Actinomycetales species, particularly with a high dose of T. inchonensis. Thus, it is concluded that the heat-killed Actinomycetales species can prevent and improve the progression of T1DM and its various complications profoundly.

Pattern of distribution of lipocalin 2 in the pancreas of diabetic rat

Lipocalin 2 (LCN2), discovered in 1989, is an innate, inducible glycoprotein and a cytokine implicated in the regulation of inflammation and iron homeostasis. Although, LCN2 was thought to be present only in neutrophils, it has now been reported to be secreted by many other cells including adipocytes, hepatocytes and more recently, osteoblasts. LCN2 induces insulin secretion and suppress appetite by activating melanocortin‐4 receptors in the hypothalamus. Even though LCN2 has been associated with glucose homeostasis, the pattern of distribution and function of LCN2 in the pancreas of an animal model of diabetes mellitus has not been elucidated. The aim of this study was to determine whether LCN2 is present in the pancreas of diabetic and non‐diabetic Wistar rats. Immunofluorescence method was used to identify the pattern of distribution of LCN2 in pancreatic islet cells of Wistar rats. Co‐localization staining was also performed to determine whether LCN2 is located with either, insulin, glucagon, somatostatin, or pancreatic polypeptide (PP) in the islets of Langerhans. Immunofluorescence study shows that a large number of cells in the central region of rat pancreatic islet contains LCN2, where it co‐localizes mainly with insulin. LCN2 does not co‐localize with any other islet peptide. The number of LCN2 and insulin‐containing cells in pancreatic islets decreased significantly (p &lt; 0.05) after the onset of diabetes. In conclusion, LCN2, a resident of pancreatic beta cells, may play a role in the regulation of insulin and glucose metabolism.Support or Funding InformationThis work was supported by UAE University Research Grant # G00002701

Launaea taraxacifolia leaf partitions ameliorate alloxan-induced pathophysiological complications via antioxidant mechanisms in diabetic rats

Launaea taraxacifolia (Wild.) Amin ex. Jeffery belongs to family Asteracaea. The plant is used for treatment of diseases and eaten as vegetable in Nigeria. This study investigated the ameliorative potentials of L. taraxacifolia leaf partitions in alloxan induced diabetic complications. Male Albino rats were divided into eleven groups of five rats each. Diabetes was induced following intraperitoneal administration of 150 mg/kg alloxan monohydrate and was treated with 200 and 300 mg/kg of each partitioned fractions. Hyperglycemia was reversed in all treated rats within seven days of treatments. Rats treated with the partitions showed significant increase in hematological parameters compared with diabetic control. N-hexane fraction had the best overall effect against oxidative stress particularly on heart and pancreas reduced glutathione (GSH), superoxide dismutase (SOD) and kidney glutathione S-transferase (GST) activities. The various degrees of degeneration observed in the kidney, liver, pancreas and heart of the untreated diabetic rats were milder in rats treated with partitions. The results therefore revealed the ameliorative potentials of the partitioned fractions of L. taraxacifolia leaf extract against diabetes mellitus complications via activation of the antioxidant enzymes.

MDA levels in the pancreas, testes, liver, and plasma of diabetic rats: The effect of snakehead (Channa striata) extract

Abstract. Abdulgani N, Hidayati D, Adinovitasary R, Oliviatie V, Sekartaji AD. 2020. MDA levels in the pancreas, testes, liver, and plasma of diabetic rats: The effect of snakehead (Channa striata) extract. Nusantara Bioscience 12: 50-54. There are several biomarkers of oxidative stress in diabetes mellitus; one of those biomarkers is Malondialdehyde (MDA). Increasing oxidative stress will cause increased tissue damage. This study was conducted to determine the effect of snakehead extract (SHE) on MDA level of pancreas, testes, liver, and plasma of alloxan-induced diabetic rats. There were 5 groups of treatments: non-diabetic rats/negative control (C-), diabetic rats/positive control (C+), and 3 SHE level of administration: 1 mL/day (low dose/LD), 1.6 mL/day (middle dose/MD) and 2.1 mL/day (high dose/HD). Alloxan-induced diabetic rats were administered with SHE extract orally every day for two weeks. The results showed that MDA levels in the testes, liver, pancreas, and plasma of diabetic rats administered with SHE and non-diabetic rats/negative controls (C-) were significantly lower (p &lt;0.05) compared to MDA levels in the testes, liver, pancreas, and plasma of diabetic rats without SHE administration (positive control/ C +). The highest dose of SHE treatment (2.1 mL/day) results in decreasing MDA levels were not significantly different (p&gt; 0.05) with the group of non-diabetic rats / negative controls (C-). The conclusion of this study was increasing SHE administration up to 2.1 mL/day result in reducing more of MDA levels in plasma, pancreas, liver, and testes of diabetic rats.

L-Carnitine different doses affect serum and pancreas tissue Antioxidative defense and histopathology in STZ-induced diabetic rats

Diabetes mellitus is a global health issue that affects life expectancy and quality. In diabetic patients, the oxidation status is increased which has detrimental effects against homeostasis. L-carnitine showed anti-oxidative effects in different tissues, and also it was reported that L-carnitine levels reduced in patients with diabetes. In this study, we examined the administration of different doses of L-carnitine on antioxidants status in diabetic rats’ serum and pancreas. Forty-eight male rats (200 ± 10 g weight) were randomly selected and divided into 6 groups (n = 8) as follow; Group I, untreated control, group II, Diabetic control, group III-VI, diabetic rats which received 50, 100, 200 and 300 mg/kg/day L-carnitine by intraperitoneal injection for 35 days, respectively. At the end of the study, blood samples and pancreas tissue were collected and histopathology and anti-oxidant status were measured. Our results showed that antioxidative capacity was reduced in the diabetic rats. Between different L-carnitine doses, 300 mg/kg/day of L-carnitine showed significant effects than other doses. The highest dose of L-carnitine (300 mg/kg/day) in this study, significantly increased TAS (Total Antioxidant Status), SOD (Superoxide Dismutase) and GPX (Glutathione Peroxidase) levels in the pancreas and serum. Also, it improved pancreas histopathology compared to the diabetic control group. We conclude that L-carnitine administration has protective effects on the pancreas and serum of STZ-induced diabetic rats, and exerts these beneficial effects through hypoglycemic and antioxidant actions.

Evaluation of Alpha-Amylase Inhibitory and Antidiabetic Potential of Novel 1,3,4-Oxadiazole Derivatives

Oxadiazoles are the heterocyclic compounds containing one oxygen and two nitrogen atoms in a five membered ring possessing a diversity of useful biological effects. 134-oxadiazole is one of the most important heterocyclic moieties has wide spectrum of therapeutic activities. The activities include anticancer antimicrobial anti-inflammatory anti- HIV antitubercular antidiabetic and antifungal. The present study aims to assess the in-vitro alpha amylase inhibitory and in-vivo antidiabetic activity of some novel 134-oxadiazole derivatives. The synthesized oxadiazole derivatives SOZDs viz. SOZD1 SOZD2 SOZD3 SOZD4 and SOZD5 were evaluated for the amylase inhibition and alloxan-induced diabetic rat model. The assessment parameters are body weight fasting blood glucose FBG lipid profile enzymes and pancreas histopathology. Alphalpha-amylase inhibition assay depicted that maximum inhibition was observed at the concentration of 7000 mugml. Furthermore SOZDs treated diabetic rats show significant depletion in serum FBG urea and creatinine levels. There were statistical differences between the diabetic control and SOZD treatment groups with regard to lipid profile enzymes. Besides SOZDs reinforce the healing of alpha and beta cells of the pancreas in diabetic rats. It is evident from results that SOZD4 and SOZD5 derivatives showed a significant improvement of body weight blood parameters and lipid profile enzymes. Hence the findings of our study delineated SOZD4 and SOZD5 could be emerging as potential molecules for diabetic patients.