Palm Oil Mill Effluent (POME) from the residue of biogas power plants generated by the Palm Oil Mill as the waste is still a problem in the processing environment and the environmental impact. However, POME waste still contains nutrients which are essential to the growth of microalgae as a growing medium. Utilization of POME can improve the growth of microalgae Botryococcus braunii compared to conventional media. By doing this project, the cost used by the palm oil mill waste management can be reduced and even be able to provide profits for palm oil mill and also can reduce the negative impact on the environment. The study was conducted at the Microalgae Laboratory of SBRC - IPB in September-Oktober 2018. Botryococcus braunii was cultivated in 1,000 ml erlenmeyer compared to standard medium as the control solution of 0.1% POME from the residue biogas power plants. Observation of Botryococcus braunii growth curve has carried out in accordance. Studies were arranged in CRD, with three replication. The results showed that the rate of Botryococcus braunii growth in POME is higher than the control for 2.7x106 sel/ml. And lipid extraction using osmotic shock method with HCl and CH3COONa as osmotic agents in concentrations of 0.1; 0.3; 0.5 M, the highest yield of 0.108 g was obtained on the osmotic agent CH3COONa 0.3 M.
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