Hummingbirds demonstrate remarkable robustness to wing defects by maintaining stable hovering during the molting process. However, mimicking these capabilities in flapping-wing robots presents significant challenges. To investigate the effectiveness of various controllers in maintaining the attitude of a hummingbird-mimetic flapping-wing robot against external disturbances under different wing defects, this study implemented a PD controller, a PID controller, and a three-loop feedback controller with a disturbance observer (3L-DOB controller). The flapping-wing robot features a pair of wings and controls its body’s yaw, pitch, and roll rotations by modulating the tension of wing membranes and the neutral positions of wing torsion. The performance of these controllers was evaluated through semi-tethered experiments on a gimbal, employing three Wing Sets: intact wings, one wing was defective, and both wings were defective. The defective wing emulated hummingbird wing during molting, in which the wing area was cropped by 14.1%. As a result, the 3L-DOB controller showed the best performance in terms of responsiveness and accuracy across all Wing Sets, while the PID controller also achieved comparable performance.