Ozone Gas Research Articles

Ozone as a Topical Treatment for Infected Dermal Wounds.

Dermal wound infections are a rising source of morbidity and mortality in patients worldwide as new and worsening complications reduce the efficacy of traditional treatments. These challenges in wound care are increasingly caused by comorbidities such as obesity and diabetes as well as surging rates of antibiotic resistance. As a result, there is an urgent need for alternative treatment options. Gaseous ozone has shown great promise as a potential new treatment for infected dermal wounds. In this brief review of current wound therapy techniques found in the literature, an in-depth discussion of the mechanisms, benefits, and results of topical ozone gas as a therapy for infected dermal wounds is presented. This includes studies of ozone applied to wounds performed in vitro, in vivo, and clinical settings, as well as the use of ozone as an adjunct therapy for increasing the efficacy of traditional treatments. The overwhelming evidence suggests that ozone exhibits significant antimicrobial properties and has been shown to promote wound healing factors, especially when applied between 5-60 ppm. As such, this promising alternative therapy warrants a significant investment of time and resources to fully utilize ozone as an effective treatment against antibiotic resistant bacteria and other rising challenges in wound treatment.

Nanoporous ZnO structure prepared by HiPIMS sputtering for enhanced ozone gas detection

A high performance ozone gas sensor is fabricated using high power impulse magnetron sputtering (HiPIMS) process with nanoporous ZnO as the sensing material which is deposited on micro electro mechanical systems (MEMS) device. The morphology and nanostructure properties of ZnO were tested with XRD, SEM, TEM, EDS and XPS. As the sensor is tested at different temperatures, the sensor showed high sensing response at 200 °C operating temperature and with 400 °C annealed sample of nanoporous ZnO sensor. Low concentrations of ozone were chosen to obtain best possible sensing response characteristics ranging from 50 ppb to 1000 ppb. At 1000 ppb, sensor recorded a high 638% sensing response at 200 °C optimal temperature. Selectivity test and effects of relative humidity data is presented in the study, where sensor had high selectivity towards O3 gas and the sensing response showed decreasing trend with increase in relative humidity. The reason behind sensing mechanism of the nanoporous ZnO sensor is explained due to the adsorption and desorption phenomenon on the surface of ZnO sensor.

Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm

Ozone is a triatomic allotropic modification of oxygen with very high oxidation potential and strong antimicrobial properties, and can be used as a disinfecting agent. The aim of this work was to investigate the effectiveness of gaseous ozone in reducing the number of bacteria and the total biomass of E. coli biofilm using different methods of quantification and detection. Biofilm of all tested clinical isolates and standard strain was grown on ceramic tiles with dimensions of 1.0 × 1.0 cm over 24 h. These plates were then treated with gaseous ozone for 1 h. After washing, CFU/cm2 was determined, ATP bioluminescence was measured with a luminometer, and the total biomass reduction was measured after crystal-violet staining. Gaseous ozone proved to be very effective in destroying the created bacterial biofilm on ceramic tiles. Treatment caused a reduction in total bacteria number of up to 2.00 log10CFU/cm2, followed by a reduction in total biomass of up to 61.40%. Inhibition rates varied from 35.80% to 99.41%, depending on the method of quantification used. All methods used in this study are effective in determining the anti-biofilm activity of gaseous ozone, but more research is needed.

Preclinical safety study of local use of the directed ozone gas flow in abdominal surgery

Purpose of the study: to assess the patho-histological changes in the intact pancreas and parietal peritoneum against the background of the directed ozone gas flow.Material and methods. The prospective study was conducted in 54 adult male Wistar rats. In 1st and 2nd groups (n = 24, each) the left pancreatic lobe and parietal peritoneum, respectively, was treated with a 40 mg/L ozone gas stream at a rate of 2 L/min for 1.5 minutes. 1, 3, 30 and 120 days after the first operation 6 animals of each groups were sacrificed and tissue specimens were collected for histological analysis. In the control group (6 sham operated animals), tissue samples were collected 120 days after laparotomy.Results. A day after treatment with ozone gas stream, the pancreatic specimens of 1st group differed from the control in moderate and mild perivascular infiltration and edema. In the period from 3 to 120 days after exposure, the pancreatic specimens of 1st group and control did not differ significantly. Specimens of 2nd group differed from the control degree of edema and infiltration in the first 3 days, the level of proliferation of fibroblast-like cells and fibrosis in 120 days after treatment of the parietal peritoneum with a directed ozone gas stream.Conclusion. Local use of the directed ozone gas flow at a concentration of 40 mg/L at a rate of 2 L/min is accompanied by the same type of patho-histological changes from the intact pancreas and parietal peritoneum in the form of mild to moderate edema, perivascular polymorphic cell infiltration in the early period after exposure. Late parietal peritoneum reaction (moderate events fibroblast-like cell proliferation and fibrosis) requires additional safety studies of intraabdominal use of ozone-oxygen mixture.

Ozone Therapy in the Management and Prevention of Caries.

The article covered the function and results of ozone treatment in managingand preventing dental caries. Specifically, the author examined ozone and its benefits, including bactericidal, analgesic, anti-inflammatory, and immunomodulatory effects. Ozonated water, ozonated olive oil, and ozone gas are forms of ozone used in dentistry. The authors provided examples of studies regarding the positive impact of ozone therapy on patients with caries. Also, the research authors described several effects of ozonated water, namely, disinfectant, anti-inflammatory, activation of intracellular metabolism of the oral mucosa and dental wounds, advancement of local blood circulation, provocation of regenerative functions, and hemostatic effect in capillary bleeding. The ozone generator and equipment for creating an ozone-oxygen (O3/O2)gas mixture were mentioned as required in dentistry to produce ozone.

Is Ozone a Valid Adjuvant Therapy for Periodontitis and Peri-Implantitis? A Systematic Review.

Ozone is a naturally occurring unstable compound with three oxygen atoms that generally transforms into an oxygen molecule, releasing one oxygen atom. This feature has been exploited in dentistry for numerous applications, including for periodontal diseases and peri-implantitis. This review was performed in relation to the PRISMA flow chart and was annotated in the PROSPERO register. PICO questions were used as research questions. The risk of bias in the non-randomized clinical trials was appraised using the ROBINS-I tool. An electronic search found a total of 1073 records, in particular, 842 from MEDLINE/PubMed, 13 from Bio Med Central, 160 from Scopus, 1 from the Cochrane library databases, and 57 from the PROSPERO register. A total of 17 studies were included in the present systematic review. Information regarding the characteristics of the periodontal clinical and radiographic parameters for gaseous ozone, ozonate water, ozonate oil, and ozone gel, including clinical attachment loss (CAL) probing depth (PPD), bleeding on probing (BoP), plaque index (PI), gingival index (GI), and marginal bone levels (MBL), were obtained. The studies included in this systematic review show different results regarding the ozone in periodontal treatment in association with or without SRP.

Ozonation with Hydrogen Peroxide for Treating Wastewater from Industrial Potato Processing - a Preliminary Investigation

ABSTRACT Wastewater generated from agricultural activities and food production is a source of environmental pollution, and its treatment is a challenging technological issue. Ozone gas (O3), and the possibility of associating it with hydrogen peroxide (H2O2), has the potential to remove persistent organic compounds in effluents. In the present work, wastewater from industrial potato processing was characterized, and its treatment with ozone combined with hydrogen peroxide was assessed. The inlet ozone concentration and the flow rate were 84.5 mg L−1 and 62.5 mL min−1, respectively. The promising conditions of temperature, pH, and H2O2/O3 proportion were determined to reduce the chemical oxygen demand, turbidity, and color, according to a central composite rotatable design. The H2O2/O3 proportions were 0.1, 0.3, 0.5, and 0.6. Additionally, the response variables were evaluated as a function of the ozone and hydrogen peroxide treatment time for up to 24 h. The conditions obtained for treating wastewater from industrial potato processing were the following: temperature = 25°C; pH = 3.0, and H2O2/O3 proportion = 0.1. For the water quality parameters adopted, the combination of ozone and hydrogen peroxide was effective at removing color and turbidity. The chemical oxygen demand, color, and turbidity reduction stabilized after 1.16 h, with cumulative applied dosages of ozone and hydrogen peroxide equal to 380 mg L−1 and 35 mg L−1, respectively.

Novel control of house fly Musca domestica and bacterial isolates by ozone gas

Ozone is a sturdy oxidant which canrub insects and microorganisms. Thus, it could be useful in eliminate the Housefly in hospitals. The purpose of test here is to determine degree of ozone exposure required to remove houseflies and reduction of pathogenic bacteria. Ozone was effective with housefly, requiring only low minutes of treatment (1 to 14 min.) at minimum concentrations of ozone gas O3 (0.125 g/m3 to 1 g/m3), the insects were eliminated the ozone. Thus, ozone exhibit potential as a fumigant for ousefly nesting materials, but moreover research is needed to evaluate its acceptability and efficacy in that field. The need for a dependable method to decontaminate housefly nesting materials as part of an overall ousefly (Musca domestica) system at hospitals sterilization is discussed. In this paper, we are using ozone gas as an alternative housefly pest and to remove house flies at hospitals, and we also illustrate data on the efficacy of ozone against the housefly (Musca domestica) and its effect on specific characteristics and properties of the treated zone application is currently attracting attention, particularly since. (a) There are no residues on the product. (b) There is no need for aeration to remove the gas. Green synthesis industrial applications and improvements in ozone technology together with new regulatory actions worldwide have emerged in last years, making it easier to use and applicable in a wide range - as the same with the pathogenic bacteria we are using ozone gas to control it, the numbers of bacterial cells remaining after ozone treatments were less than for untreated cells. The lowest dose of ozone gas for complete reduction of pathogenic bacteria was 0.125 g/m3 after 24 h of incubation.

Ozone and Nitrogen Dioxide Pollutants Detection System Based on IoT

Air pollution is one of the main causes of health problem in urban areas. Vehicles are the major sources of the air pollution in urban cities. To prevent the air pollution situation from increasing, we have developed an advanced outdoor air quality measurement system using a ESP8266 microcontroller and senores. It can be placed at crowded automobile locations to monitor pollutants emitted from vehicles. Ozone gas (O3) and nitrogen dioxide (NO2) was measured in this study. The system provides continuous monitoring of vehicle air pollution with a high time accuracy.

Effect of Ozone Treatments on Germination and Disinfection of Sacha Inchi (Plukenetia volubilis L.) Seeds

ABSTRACT The objectives were to evaluate the ozonation effects on germination and disinfection of sashi inchi seeds. Seeds groups were treated applying concentrations of gaseous ozone of 5 and 20 mg/L for 20 min. Others were immersed in water with dissolved ozone (0.5 and 1.0 mg/L) during 15 min. Ozone did not register negative effects on germination or on the quality of the seeds. Treatments with lower ozone concentrations recorded the best results in germination percentage and vigor indicator (86%, 94% and 84%). The gaseous phase was the most suitable one for treating the seeds (5 mg/L for 20 min). Only a treated group reached greater seedlings survival (53%), resisting the fungal attack. Applied disinfection allowed reducing the microbial load.

Etching of molybdenum via a combination of low-temperature ozone oxidation and wet-chemical oxide dissolution

Etching of molybdenum was demonstrated in two steps. Mo was first oxidized in an ozone gas ambient to form molybdenum oxide. It is shown that comparable oxide thicknesses can be obtained in ozone and oxygen but at lower temperatures for the former. Initial oxide growth is fast but then considerably slows down due to its diffusion-limited character. The metal-oxide thickness can be controlled by temperature and defines the amount of metal etch per cycle (EPC). XPS analysis showed that the thermally grown oxide is MoO3. In the second, wet-chemical step, MoO3 was dissolved selectively toward the Mo metal using an aqueous solution. The dissolution rate of amorphous MoO3 formed in O3 at temperatures below ∼230 °C is fast, but the dissolution of MoO3 formed at Tox > 230 °C was shown to be incomplete. Cross-section TEM showed a matrix of amorphous oxide with crystallized MoO3 islands, the latter more difficult to dissolve. However, the crystalline phase could be completely and selectively removed using a more concentrated NH4OH solution at an elevated temperature (70 °C). The EPC was determined for temperatures between 150 and 290 °C. The etch rates increased with temperature from 1–2 nm/cycle at 150 °C to 5–6 nm/cycle at 290 °C. This hybrid thermal-wet etching sequence is well suited for vertical and lateral recess etching as it shows a controlled and isotropic dissolution of polycrystalline Mo at the nanoscale. Furthermore, the process shows a progressive surface smoothening upon increasing the number of etching cycles.

Reactivity enhancement of natural gas/diesel RCCI engine by adding ozone species

Reactivity controlled compression ignition (RCCI) is an alternative combustion strategy with the potential of significant advantages in terms of increasing thermal efficiency together with reduction of NOx and soot emissions. The RCCI engines have been encountered not to be applicable broadly since they suffer from some difficulties mainly contributed to the controllability of combustion and limited operating ranges. The difficulties become even worse where low-reactivity fuels like natural gas (NG) are applied. Ozone gas, a chemical species with an extreme level of reactivity, can improve the combustion efficiency along with the advancement of combustion phasing in the RCCI engine. In this study a multidimensional computational fluid dynamic (CFD) which is coupled with proper detailed chemical kinetic mechanisms is employed to investigate the influence of ozone addition (i.e., 10, 100 and 1000 ppm) to air-fuel mixture with different initial conditions (e.g., intake temperature and equivalence ratio), on the performance and emissions characteristics of the RCCI engine. The results imply that the addition of even low concentrations of ozone (10 ppm) have considerable influences on the RCCI combustion characteristics. Addition of ozone by a distinct quantity not only can enhance the combustion phasing controllability, but also can extend the operating range of the RCCI engine in both lower intake air temperature and the lower fraction of the high-reactive fuel. From the results it is conceived that by adding 1000 ppm ozone into the air-fuel mixture, it is possible to reduce the diesel fuel fraction from 20% to 10% and intake air temperature from 355 K to 335 K, respectively.

Accelerated Sulfur Oxidation by Ozone on Surfaces of Single Optically Trapped Aerosol Particles

The sulfur oxidation in mixed sodium thiosulfate/sucrose/aqueous microdroplets by gaseous ozone is studied in this work via aerosol optical tweezers coupled with Raman spectroscopy, which can simultaneously determine various physicochemical properties and the heterogeneous reaction kinetics of single optically trapped microdroplets, allowing for elucidating their complicated interplay. According to the kinetics measurement results at different relative humidities, ozone concentrations, and stoichiometries of inorganic and organic solutes, this work finds that a high aerosol ionic strength can accelerate the ozone oxidation of thiosulfate at air–water interfaces, while a high aerosol viscosity prolongs the reaction time scales due to diffusion-limited kinetics. The kinetic multilayer model of aerosol surface and bulk chemistry (KM-SUB) is utilized to investigate the observed heterogeneous kinetics results and to retrieve the surface reaction rate coefficients. The KM-SUB model fit results indicate that the observed kinetics of sulfur oxidation in binary sodium thiosulfate aqueous microdroplets with high ionic strengths is dominated by interfacial reactions, and the fitted surface reaction rate coefficients increase 1 order of magnitude when the droplet ionic strength increases around 40 M. This work further utilizes the kinetics measurements of ozone dependence to discuss the coupling among properties of ozone gas-interface-bulk partitioning and surface reaction rate in the kinetics model. Finally, this work also exploits the surface reaction of thiosulfate with ozone to retrieve the aerosol viscosity of microdroplets containing ternary aqueous sodium thiosulfate and sucrose mixtures, demonstrating the feasibility of pinning down aerosol viscosities via reaction kinetics.

Detoxification of unshelled hazelnut artificially contaminated with aflatoxins by gaseous ozone

Detoxification of unshelled hazelnut artificially contaminated with aflatoxins by gaseous ozone

Photochemical advanced oxidative process treatment effect on the pesticide residues reduction and quality changes in dried red peppers

Pesticide residues in crops are widely monitored, and the residue reduction techniques at the post-harvest stage are important to maintain food safety. In dried crops, pesticide residues can be concentrated after dehydration, which increases concerns regarding residue risk. Therefore, the residue reduction effects of ultraviolet (UV), ozone, and photochemical advanced oxidative process (pAOP) were investigated for dried peppers at the post-harvest stage. UV254 treatment reduced 59.7% of the residue concentration on average, while UV360 showed a reduction of only 13.3% under 9.6 W m−2 of UV exposure for 24 h. Gaseous ozone treatments reduced the residue concentrations up to 57.9% on average. In contrast, the pAOP treatment reduced the concentration up to 97% and was superior to UV or ozone treatment alone. Increased drying temperature under pAOP condition resulted in higher reduction ratios at 40–80 °C. The pAOP conditions with 12 and 24 µmol/mol of ozone and UV254 irradiation for 24–48 h reduced the residue concentrations to 39–67%. Particularly, difenoconazole, fludioxonil, imidacloprid, and thiamethoxam residue concentrations were drastically reduced by over 50% under 12 µmol/mol ozone of the pAOP condition, while carbendazim, fluquinconazole, and pyrimethanil were relatively stable and their concentrations reduced below 50% under 24 µmol/mol ozone of the pAOP treatment. Various drying-related quality parameters of drying peppers such as water-soluble color, capsanthin, capsaicinoids, acid value, peroxide value, and thiobarbituric acid value were slightly altered, but not significantly, under 12 µmol/mol ozone of the pAOP condition, while the peroxide value was significantly altered under the higher ozone conditions. Therefore, pAOP treatment combined with gaseous ozone can be used for reducing residual pesticides in peppers without greatly reducing quality.

Histomorphometric evaluation of skin wounds in rats submitted to biomodulatory therapies – research protocol

INTRODUCTION: The use of biomodulatory therapies in order to help tissue repair has been increasingly common in different areas of health. OBJECTIVE: This study aims to comparatively evaluate the effects of 660 nm laser photobiomodulation, ozone therapy, and ozonated oil on repair through histomorphometric analysis in skin wounds in rats. Forty Wistar rats will be divided into 4 groups of 10 animals each, Control Group (GC), Laser Group (LG), Ozone Gas Group (OGG), and Ozonated Oil Group (OOG). MATERIALS AND METHODS: Standard skin wounds will be made on the back of the animals, and the different experimental groups will be treated with the biomodulatory therapies described for three consecutive days. Five and ten days after surgery, five rats from each group will be euthanized. Skin fragments, including the wound area, will be removed for histological processing and subsequent staining of histological sections with Hematoxylin-eosin and Sirius red. Micrographs of the histological sections will be obtained and ten standard images will be captured for quantitative evaluation of the variables collagen area, number of blood vessels, and epithelium thickness. The variables infiltrate of polymorphonuclear and monomorphonuclear inflammatory cells, as well as edema, will be analyzed semiquantitatively. Statistical analysis of the study variables will be performed, with a significance level of p<0.05. CONCLUSION: It is expected to verify which of the biomodulatory therapies used can favor the resolution of tissue repair, in particular, by promoting collagen biosynthesis.

Field trial assessing the antimicrobial decontamination efficacy of gaseous ozone in a public bus setting

The widespread COVID-19 pandemic caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) necessitated measures aimed at preventing the spread of SARS-CoV-2. To mitigate the risk of fomite-mediated transmission, environmental cleaning and disinfection regimes have been widely implemented. However, conventional cleaning approaches such as surface wipe downs can be laborious and more efficient and effective disinfecting technologies are needed. Gaseous ozone disinfection is one technology which has been shown to be effective in laboratory studies. Here, we evaluated its efficacy and feasibility in a public bus setting, using murine hepatitis virus (a related betacoronavirus surrogate) and the bacteria Staphylococcus aureus as test organisms. An optimal gaseous ozone regime resulted in a 3.65-log reduction of murine hepatitis virus and a 4.73-log reduction of S. aureus, and decontamination efficacy correlated with exposure duration and relative humidity in the application space. These findings demonstrated gaseous ozone disinfection in field settings which can be suitably translated to public and private fleets that share analogous characteristics.

Inactivation of nontuberculous mycobacteria by gaseous ozone treatment

Nontuberculous mycobacteria (NTM) are environmental bacteria resistant to many common disinfectants and ultraviolet radiation. Inhalation of aerosols generated from NTM-containing water and soil causes NTM lung disease, especially in people with underlying lung diseases and decreased immunity. To prevent healthcare-acquired NTM infections, it is important to eradicate NTM living in hospital environments. Therefore, we evaluated the efficacy of gaseous ozone for the inactivation of NTM, namely Mycobacterium (M.) avium, M. intracellulare, M. kansasii, M. abscessus subsp. abscessus and M.abscessus subsp. massiliense. Gaseous ozone treatment at 1 ppm for 3 h reduced the bacterial number of all strains by more than 97%. Gaseous ozone treatment could be a practical, effective and convenient disinfection method for NTM living in hospital environments.

Disinfection of polyvinyl siloxane impression material using ozone gas, 0.1% riboflavin, glutaraldehyde, and microwave irradiation and their effect on physical properties.

To assess the antimicrobial effect of ozone gas (OG) 0.1% riboflavin (RF), glutaraldehyde (GaH), and microwave irradiation (MI) on Polyvinyl siloxane impression material formerly colonized with E. coli, P. Aeruginosa, E. faecalis, and S.aureus and their effect on the physical properties MATERIAL AND METHODS: One hundred disk-shaped specimens (diameter 10mm and thickness 3mm) were developed by using polyvinylsiloxane impression material and inoculated by the American Type Culture Collection (ATCC) of E. coli, P. aeruginosa, E. faecalis, and S.aureus in an in-vitro setup. The samples were arbitrarily isolated into five groups and subjected for 3min to the designated disinfection modality. Group 1 Control, group 2: GaH, group 3: 0.1% RF, group 4: MI, and group 5 OG. Following decontamination, the physical properties (contact angle, strain-in-compression, and tear strength) of impression materials were evaluated. Statistical analysis for CFU/mL (log10) for exposed E. coli, P. aeruginosa, E. faecalis, and S.aureus was performed by using analysis of variance (ANOVA) and Tukey's multiple comparison tests at a p-value of less than 0.05. The most effective antimicrobial efficacy for impression disinfection against all scrutinized microbial colonies were displayed by group 5 OG and the lowest disinfection effectiveness was unveiled by the control group with no treatment. The intragroup comparison revealed that impression disinfection with GaH, MI, and ozone corroborated analogous antimicrobial efficacy(p>0.05). The disinfecting capacity of the impression material with 0.1% RF was significantly less than MI, GaH, and OG (p<0.05). Disinfection of polyvinyl siloxane impression material with ozone gas, microwave irradiation, and glutaraldehyde demonstrated reasonable antimicrobial efficiencies against E. coli, P. aeruginosa, E. faecalis, and S.aureus with no detrimental effects on the physical properties of impression material. More studies are advocated to extrapolate the findings of the present study.

Ozone Decontamination of Medical and Nonmedical Devices: An Assessment of Design and Implementation Considerations.

The control of infectious diseases can be improved via carefully designed decontamination equipment and systems. Research interest in ozone (a powerful antimicrobial agent) has significantly increased over the past decade. The COVID-19 pandemic has also instigated the development of new ozone-based technologies for the decontamination of personal protective equipment, surfaces, materials, and indoor environments. As this interest continues to grow, it is necessary to consider key factors affecting the applicability of lab-based findings to large-scale systems utilizing ozone. In this review, we present recent developments on the critical factors affecting the successful deployments of industrial ozone technologies. Some of these include the medium of application (air or water), material compatibility, efficient circulation and extraction, measurement and control, automation, scalability, and process economics. We also provide a comparative assessment of ozone relative to other decontamination methods/sterilization technologies and further substantiate the necessity for increased developments in gaseous and aqueous ozonation. Modeling methodologies, which can be applied for the design and implementation of ozone contacting systems, are also presented in this review. Key knowledge gaps and open research problems/opportunities are extensively covered including our recommendations for the development of novel solutions with industrial importance.