ABSTRACT Soft-nose, as one of the physiological disorders of mango fruit, results in a reduction of the commodity rate. In this paper, the results showed that reactive oxygen species (ROS) accumulated capacity was higher than the scavenging ability of ROS in soft-nose mango fruit since ROS generation. Meanwhile, fatty acid content and the expression of genes involved in fatty acid metabolism in the soft-nose fruit increased. α-linolenic acid and jasmonic acid (JA) particularly increased, the activities of allene oxide synthase (AOS) and allene oxide cyclase (AOC) increased, and the expressions of MiPLDβ2, MiFAD3, MiACSL7 and MiACOX1 up-regulated in soft-nose mango fruit. Thus, ROS accumulation along with lipid peroxidation and JA may be associated with the development of postharvest soft-nose disorder in mango fruit.