Outstanding Antibacterial Properties Research Articles

Antibacterial properties of enzymatically treated PET fibers functionalized by nitric oxide

At present, microbes have enormous potential to become a major global public health issue. For example, Escherichia coli is the prominent cause of cholecystitis, urinary tract infections, and other infections. Due to its outstanding antibacterial properties, nitric oxide (NO) is essential for biological processes. Additionally, enzymatic hydrolysis using polyethylene terephthalate hydrolase (PETase) is one of the promising methods for PET upcycling. First, recombinant PETase was used to enzymatically treat waste PET fibers, and polyethylenimine (PEI) was added as a secondary amine donor. Subsequently, the aminated PET fiber was inserted into a reactor charged with NO gas (10 atm, 3 days) to obtain N-diazeniumdiolate (NONOate) products that can inhibit bacteria growth. In this study, the first strategy for antibacterial applications by NO-releasing PETase-hydrolyzed PET fibers was demonstrated. NO-conjugated PET fibers were successfully prepared which exhibits a continuous NO release profile over 12 h. The surface properties of functionalized PET fibers were successfully confirmed by fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and Griess assay. The antibacterial test indicated a reduction of Escherichia coli by 90.2% and Staphylococcus aureus by 71.1% after exposure to the functionalized material. Therefore, this novel antibacterial agent may offer great potential applications in the medical field.

Injectable hydrogels activated with copper sulfide nanoparticles for enhancing spatiotemporal sterilization and osteogenesis in periodontal therapy.

Developing biomaterials capable of promoting bone regeneration in bacteria-infected sites is of utmost urgency for periodontal disease therapies. Here we produce a hybrid hydrogel by integrating CuS nanoparticles (CuSNPs), which could kill bacteria through photothermal therapy (PTT) triggered by a near infrared (NIR) light, and a gelatin methacryloyl (GelMA) hydrogel, which is injectable and biocompatible. Specifically, CuSNPs were precipitated by chitosan (CS) firstly, then grafted with methacrylic anhydride (MA) to form CuSNP@CS-MA, which was photo-crosslinked with GelMA to synthesize hybrid hydrogels (GelMA/CuSNP). The hybrid hydrogels exhibited a broad-spectrum antibacterial property that could be spatiotemprorally manipulated through applying a NIR light. Their mechanical properties were adjustable by controlling the concentration of CuSNPs, enabling the hydrogels to become more adapted to the oral diseases. Meanwhile, the hybrid hydrogels showed good cytocompatibility in vitro and improved hemostasis in vivo. Moreover, they accelerated alveolar osteogenesis and vascular genesis, successfully treating periodontis in four weeks in a rat model. GelMA/CuSNP hydrogels showed a broad-spectrum sterilization ability via PTT in vitro and outstanding antibacterial property in vivo, suggesting that the hybrid hydrogels could function in the challenging, bacteria-rich, oral environment. Such injectable hybrid hydrogels, capable of achieving both facilitated osteogenesis and NIR-inducible sterilization, represent a new biomaterial for treating periodontitis.

Injectable, thermo-sensitive and self-adhesive supramolecular hydrogels built from binary herbal small molecules towards reusable antibacterial coatings.

Herbal hydrogels as a new class of sustainable functional materials have attracted extensive attention. However, the development of herbal hydrogels is significantly hindered due to their poor hydrogel performances and the lack of universal preparation methods. In this study, four herbal hydrogels composed of phytochemical polyphenols and stevioside compounds are prepared through a facile heating-cooling process, where multiple hydrogen bonding interactions between two monomers provide the main driving force for gelation. These herbal hydrogels exhibit thermo-sensitivity and good reversibility (25-90 °C), robust adhesion behaviours on hydrophilic and hydrophobic surfaces (maximum adhesion strength of 591.7 kPa), and outstanding antibacterial properties (100% bacteriostatic ratio). Profiting from these intriguing characteristics, they are demonstrated to show great potential as natural antibacterial coatings by depositing thin hydrogel layers onto diverse substrates. More importantly, the hydrogel coatings could be easily recycled by thermal regelation and reused at least 5 times. This work proposes a simple and universal strategy for preparing functional hydrogels based on binary herbal small molecules, which also sheds light on the development of reusable hydrogel coatings.

Regulation of band gap and localized surface plasmon resonance by loading Au nanorods on violet phosphene nanosheets for photodynamic/photothermal synergistic anti-infective therapy.

In the face of the serious threat to human health and the economic burden caused by bacterial antibiotic resistance, 2D phosphorus nanomaterials have been widely used as antibacterial agents. Violet phosphorus nanosheets (VPNSs) are an exciting bandgap-adjustable 2D nanomaterial due to their good physicochemical properties, yet the study of VPNS-based antibiotics is still in its infancy. Here, a composite of gold nanorods (AuNRs) loaded onto VPNS platforms (VPNS/AuNR) is constructed to maximize the potential of VPNSs for antimicrobial applications. The loading with AuNRs not only enhances the photothermal performance via a localized surface plasmon resonance (LSPR) effect, but also enhances the light absorption capacity due to the narrowing of the band gap of the VPNSs, thus increasing the ROS generation capacity. The results demonstrate that VPNS/AuNR exhibits outstanding antibacterial properties and good biocompatibility. Attractively, VPNS/AuNR is then extensively tested for treating skin wound infections, suggesting promising in vivo antibacterial and wound-healing features. Our findings may open a novel direction to develop a versatile VPNS-based treatment platform, which can significantly boost the progress of VPNS exploration.

Preparation of polyethersulfone ultrafiltration membrane coated natural additives toward antifouling and antimicrobial agents for surface water filtration

Chitosan (CS) and polyphenon 60 from green tea (PGT) as natural additives were used in the preparation of antifouling and antibacterial polyethersulfone (PES) ultrafiltration membranes via the surface coating method. The effect of additive coating on fouling resistance and antibacterial properties were evaluated. The membrane characterizations were carried out using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, and surface hydrophilicity by contact angle. The results showed that the optimized modified membrane (1 wt% CS/3 wt% PGT-coated PES, denoted as CS1PGT3) demonstrated better hydrophilicity than the pristine PES membrane. In addition, the CS1PGT3 membrane exhibited high BSA rejection (99.99%), relatively high flux recovery (78.78%), and the highest water flux (46.83 L/m2.h) among all modified PES membranes. After the CS1PGT3 membrane was immersed in different soaking solutions (i.e., acid, alkaline, and neutral) for 21 days, the stability of CS and PGT groups on the PES membrane was also confirmed. Moreover, during examination using surface water filtration, the removal rates of chemical oxygen demand, turbidity, and aromatic compounds were increased from 64.30%, 34.23%, and 62.78% to 88.22%, 64.18%, and 84.21%, respectively. The largest inhibition zone diameter (7.83 mm of E. coli) and highest antibacterial efficiency (99% of E. coli) were achieved, suggesting the outstanding antibacterial properties of the optimized modified membrane. Thus, the incorporation of CS/PGT into PES membrane via the surface coating showed promising method to enhance antifouling and antibacterial properties.

Cellulose nanofibers embedded chitosan/tannin hydrogel with high antibacterial activity and hemostatic ability for drug-resistant bacterial infected wound healing

Millions of patients annually suffer life-threatening illnesses caused by bacterial infections of skin wounds. However, the treatment of wounds infected with bacteria is a thorny issue in clinical medicine, especially with drug-resistant bacteria infections. Therefore, there is an increasing interest in developing wound dressings that can efficiently fight against drug-resistant bacterial infections and promote wound healing. In this work, an anti-drug-resistant bacterial chitosan/cellulose nanofiber/tannic acid (CS/CNF/TA) hydrogel with excellent wound management ability was developed by electrospinning and fiber breakage-recombination. The hydrogel exhibited an outstanding antibacterial property exceeding 99.9 %, even for drug-resistant bacteria. This hydrogel could adhere to the tissue surface due to its abundant catechol groups, which avoided the shedding of hydrogel during the movement. Besides, it exhibited extraordinary hemostatic ability during the bleeding phase of the wound and then regulated the wound microenvironment by absorbing water and moisturizing. Moreover, the CS/CNF/TA also promoted the regrowth of vessels and follicles, accelerating the healing of infected wound tissue, with a healing rate exceeding 95 % within a 14-day timeframe. Therefore, the CS/CNF/TA hydrogel opens a new approach for the healing of drug-resistant bacterial infected wounds.

Industrial Cannabis sativa: Role of hemp (fiber type) in textile industries

This review paper highlights the importance and role of hemp fibre in textile industries. In recent years, there has been a resurgence of interest in hemp as a sustainable and versatile textile fibre in fashion and textile industry. Hemp is a sustainable and environmental friendly crop that can provide valuable raw materials to a large number of industrial applications. Hemp fibre is very strong compared with other natural fibres such as cotton, flax and nettle. The cultivation of hemp has significantly less environmental impact compared to cotton. The industrial hemp contains primary and secondary fibres of which the primary fibres are utilisable for the textile end use. Hemp has been the mostly blended with cotton and synthetic fibres due to barriers in the industrial process of the production of full hemp-based textiles. Hemp clothing is stronger and more durable than cotton clothing and does not deform as easily. Apparel made from hemp merges easily with dyes and does not discolour easily. Hemp has outstanding antibacterial properties that surpass those of cotton and also any other natural fibre. Hemp textile fibre is hypoallergenic and has natural antimicrobial properties, making it an excellent choice for individuals with sensitive skin. However, despite its many benefits, hemp is still relatively expensive in India compared to other cellulosic fibres such as cotton, linen, and rayon etc. Hemp has a great cultural and historical value in India because it has been grown and used here for thousands of years. This cultural history can be conserved and honoured by using hemp in the fashion and textile business.

A novel strategy for the preparation of multifunctional and robust polyhydroxyalkanoates/GQDs nanocomposites with UV-resistance, pH-responsive and antibacterial properties

Biosynthesized polyhydroxyalkanoates (PHAs) offer several compelling advantages, including biodegradability, biocompatibility, and suitability for use in environmentally friendly packaging materials. However, poor crystallization ability and lack of functionality (e.g., UV-resistance and antibacterial properties) significantly restrict the practical applications of PHAs. In this study, graphene quantum dots (GQDs) containing with rich amino groups, carboxy groups and conjugate structure were tailor-made as multifunctional nanofillers to prepare PHAs/GQDs composites. The GQDs not only exhibited excellent dispersion within the PHAs matrix at the nanoscale but also act as nucleating agents to dramatically increase the nucleation density of PHAs. Remarkably, the incorporation of just 0.5 wt% GQDs led to outstanding antibacterial performance (99.99%), UV resistance (90.50%), transparency (>80%) and mechanical properties in the PHAs composite. ​Moreover, these PHAs composites displayed strong fluorescence stability and demonstrated sensitivity to pH variations. Building upon these findings, we propose a novel concept for a wearable device capable of intelligently detecting changes in sweat pH during human exercise. This work presents a straightforward and effective method for preparing multifunctional GQDs, thereby expanding the potential applications of PHAs in the realm of green packaging and intelligent sensing.

Replicated biopolymer pattern on PLLA-Ag basis with an excellent antibacterial response

The design of functional micro or nanostructured surfaces is undergoing extensive research for their intriguing multifunctional properties and for large variety of potential applications in biomedical field (tissue engineering or cell adhesion), electronics, optics or microfluidics. Such nanosized topographies can be easily fabricated by various lithography techniques and can be also further reinforced by synergic effect by combining aforementioned structures along materials with already outstanding antibacterial properties. In this work we fabricated novel micro/nanostructured substrates using soft lithography replication method and subsequent thermal nanoimprint lithography method, creating nanostructured films based on poly (l-lactic acid) (PLLA) fortified by thin silver films deposited by PVD. Main nanoscale patterns were fabricated by replicating surface patterns of optical discs (CDs and DVDs), which proved to be easy, fast and inexpensive method for creating relatively large area patterned surfaces. Their antimicrobial activity was examined in vitro against the bacteria Escherichia coli and Staphylococcus epidermidis strains. The results demonstrated that nanopatterned films actually improved the conditions for bacterial growth compared to pristine PLLA films, the novelty is based on formation of Ag nanoparticles on the surface/and in bulk, while silver nanoparticle enhanced and nanopatterned films exhibited excellent antibacterial activity against both bacterial strains, with circa 80 % efficacy in 4 h and complete bactericidal effect in span of 24 h.

Effects of durable antibacterial, UV-resistant cotton fabrics with differentiated polydopamine metal complexes

Antibacterial cotton fabrics with metal derivatives often faced the low durability and poor multifunction. Here, a variety of metal ions (Fe3+, Ca2+, Zr4+, Zn2+, Co2+, and Ni2+) were utilized in synergy with biomass polydopamine (PDA) to prepare durable antibacterial and UV-resistant cotton fabrics (PM@CFs). Owing to the difference in chelating ability and characteristics of metal ions themselves, polydopamine metal complexes coatings endowed cotton fabrics with well and differentiated antibacteria and ultraviolet (UV) resistance, respectively. More importantly, all PM@CFs still maintained excellent mechanical properties and air permeability with respect to the original cotton fabrics. Among them, polydopamine zinc complex exhibited comprehensive and outstanding antibacterial property and durability due to the strong chelating ability and multi-antibacterial mechanisms of Zn2+, in which the antibacterial rates against both E. coli and S. aureus achieved 99.9 % even after 50 washing cycles.

Carbon Dots' Potential in Wound Healing: Inducing M2 Macrophage Polarization and Demonstrating Antibacterial Properties for Accelerated Recovery.

Bacterial infections and persistent inflammation can impede the intrinsic healing process of wounds. To combat this issue, researchers have delved into the potential use of carbon dots (CDs) in the regulation of inflammation and counteract infections. These CDs were synthesized using a microwave-assisted hydrothermal process and have demonstrated outstanding antibacterial and antibiofilm properties against Gram-positive and Gram-negative bacteria. Additionally, CDs displayed biocompatibility at therapeutic concentrations and the ability to specifically target mitochondria. CD treatment effectively nullified lipopolysaccharide-triggered reactive oxygen species production by macrophages, while simultaneously promoting macrophage polarization toward an anti-inflammatory phenotype (M2), leading to a reduction in inflammation and an acceleration in wound healing. In vitro scratch assays also revealed that CDs facilitated the tissue-repairing process by stimulating epithelial cell migration during reepithelialization. In vivo studies using CDs topically applied to lipopolysaccharide (LPS)-stimulated wounds in C57/BL6 mice demonstrated significant improvements in wound healing due to enhanced fibroblast proliferation, angiogenesis, and collagen deposition. Crucially, histological investigations showed no indications of systemic toxicity in vital organs. Collectively, the application of CDs has shown immense potential in speeding up the wound-healing process by regulating inflammation, preventing bacterial infections, and promoting tissue repair. These results suggest that further clinical translation of CDs should be considered.

Open roads and bridge: Preservation of fresh beef by a packaging film constructed from photosensitizing bacterial cellulose

Food packaging is now widely used in everyday life to protect food from certain environmental factors. In this work, we have successfully prepared a bacterial cellulose/chitosan-TPE-COOH composite film (BC/CS-TPE), which can achieve broad-spectrum killing of bacteria through a variety of antibacterial mechanisms and ensure the freshness of the beef. In this complex film, CS acts as “advance team”, responsible for breaking through the cell wall or outer membrane of bacteria, while the reactive oxygen species produced by photosensitizer under irradiation attacks bacteria, further increasing the destructive effect on the bacterial cell membrane. This allows the film to have outstanding antibacterial properties that can kill 108 CFU/mL pathogens in 10 min. At the same time, cell experiments and hemolysis experiments proved that the film has good biocompatibility. Therefore, BC/CS-TPE film, as the efficient functional food packaging film, has a broad future application prospect.

Green synthesized clavate-shaped ZnO modified silk fabric with aging resistance and outstanding antibacterial and UV-shielding properties

Silk, a popular luxury textile with many years of history, is easy to embrittle and adsorb bacteria when stored for a long time or exposed to sunlight, which affects its service life. To improve the silk fabrics with excellent antibacterial activity, washing and aging durability, and UV shielding, multifunctional silk fabrics were designed and fabricated via a green finishing approach. Here, we proposed a simple strategy to fabricate silk fabrics with multi-functions via coating clavate-shaped ZnO nanoparticles (ZF-silk fabrics) by dipping and pressing for cycles. The scanning electron microscope (SEM), fourier transform infrared (FTIR), ultraviolet visible spectrophotometry (UV–Vis), and X-ray diffraction (XRD) were used to analyze the structure and properties of fabricated ZF-silk fabrics. Compared with raw silk fabric, the fabricated ZF-silk fabrics represented excellent UV shielding, good tensile strength, and superior antibacterial activity. Notably, the ZF-silk fabrics had good washing stability and aging resistance. In addition, the ZF-silk fabrics possessed superior antibacterial activity before and after aging treatment (under UV radiation for 72 h). Therefore, the durable and multifunctional ZF-silk fabrics indicate great potential for achieving to realize a long service life of the fabrics.

Preparation of Highly Effective Antibacterial Cotton Fabrics Based on Bifunctional Reactive N-Halamine

Two novel halogenated triazine amine N-halamine antimicrobial precursors, sulfuric acid mono-(2-{4-[4-chloro-6-(2,2,6,6-tetramethyl-piperidin-4-yloxy)-[1,3,5]triazin-2-ylamino]-benzenesulfonyl}-ethyl) ester sodium (PT) and sulfuric acid mono-(2-{4-[4-(3-pyridinecarboxylic sodium)-6-(2,2,6,6-tetramethyl-piperidin-4-yloxy)-[1,3,5]triazin-2-ylamino]-benzenesulfonyl}-ethyl) ester sodium (CPT), were designed, synthesized, and applied onto cotton fabrics to obtain antibacterial properties. The molecular structures of PT and CPT contained two reactive groups of vinyl sulfone and nicotinic acid, which increased the reaction with cotton fabrics compared with other antibacterial agents. The dyeing process of reactive dye was selected as the finishing method due to the similar structures of antibacterial agents to bifunctional group reactive dyes. The treated cotton fabrics were chlorinated with sodium hypochlorite to obtain antibacterial functionality. The chlorinated fabrics achieved outstanding antibacterial properties against Escherichia coli O157:H7 and Staphylococcus aureus with short contact time. Moreover, the stability measurement exhibited that the chlorine on the samples could be repaired by the diluted sodium hypochlorite solution after washing and long-time storage. In addition, the mild low-temperature process reduced fabric damage, and only about 10% and 15% of the original tensile strength was missing after finishing and chlorination in both the warp and weft directions. Compared to the traditional pad–dry–cure technique, the process developed of antimicrobial cotton had some advantages such as low salt, energy savings, and maintaining tensile strength.

Synthesis and growth mechanism of starfish-like anatase TiO2 nanocrystal with outstanding antibacterial property and super-hydrophobicity

Synthesis and growth mechanism of starfish-like anatase TiO2 nanocrystal with outstanding antibacterial property and super-hydrophobicity

In-situ constructing amidoxime groups on metal-free g-C3N4 to enhance chemisorption, light absorption, and carrier separation for efficient photo-assisted uranium(VI) extraction

The development of inexpensive and efficient semiconductor catalysts for photo-assisted uranium extraction from seawater remains a huge challenge. Herein, we have successfully synthesized amidoxime-rich g-C3N4 (AO-C3N4) by simply amidoximing a cyano-rich precursor for photo-assisted uranium extraction from seawater. The amidoxime groups not only served as the U(VI) binding sites for efficient uranium adsorption, but also significantly improved the visible light absorption capacity and carrier separation efficiency via introducing defect energy level, resulting in the excellent photocatalytic activity for AO-C3N4 towards photo-assisted uranium extraction. In the process of photo-assisted uranium extraction, U(VI) was first adsorbed by the amidoxime groups on the AO-C3N4 and then reduced to U(IV), while (UO2)O2·2H2O and (UO2)O2·4H2O were further formed by the oxidation of U(IV) by superoxide radicals (·O2-). Moreover, the generated reactive oxygen species (ROS) under light endowed AO-C3N4 with outstanding antibacterial properties, preventing the limitation of uranium extraction capacity from marine biofouling.

The in vitro effects of probiotic bacteria on genital pathogens of female dogs

BackgroundAn important aspect in the microbiology of the reproductive system of small animals is the potential occurrence of probiotic bacteria, such as lactic acid bacteria (LAB) of the genus Lactobacillus. The presence of these microorganisms is significant due to their strong antibacterial and antifungal properties. This study aimed to select probiotic strains from the oral cavity and vagina that have outstanding antibacterial properties against typical genital pathogens of the female dog reproductive tract.ResultsThe antagonistic activity of ten LAB strains was tested against seven etiological agents isolated from the genital tract of female dogs with signs of inflammation. LAB strains with the greatest ability to inhibit the growth of indicator bacteria were Lactobacillus plantarum and L. acidophilus, while L. fermentum and L. brevis strains inhibited growth the least. Almost all strains showed a complete lack of adherence to Caco-2 epithelial cells.ConclusionsAll tested LAB isolates inhibited the in vitro growth of either Gram-positive or Gram-negative pathogens, suggesting that potential probiotic strains could contribute to the balance of the normal vaginal microbiota. Furthermore, they could be considered for use as prophylactic agents or as an alternative to antibiotic therapy for infections in dogs.

Simple surface low temperature grafting for antibacterial and anti-felting inkjet printing wool fabrics

It is a trouble to solve the problem of wool fabric shrinkage in daily washing. Coating finishing on wool fiber surface can effectively reduce shrinkage rate. In this study, wool fabrics were pretreated with H2O2 and papain. Then the pretreated wool fabric was coated with chitosan to prepare antibacterial and anti-felting fabric by employing a simple low temperature grafting method. The coated wool fabric showed expectant hydrophilic property without changing the original whiteness. Dye permeability and contour sharpness of inkjet printing wool fabrics were improved by grafting chitosan compared to pretreated wool fabric. The K/S values of wool fabric increased by 40.8 % and stayed high resolution in printed pattern. The area shrinkage of wool fabric decreased from 11.5 % to 4.7 %, which was obviously lower than “machine washable” index (8 %). What's more, the existence of chitosan coating onto wool fibers resulted in outstanding antibacterial property against E. coli in the inhibition ring test. This work opens up a pathway to fabricate anti-felting and antibacterial wool fabrics in practical production with chitosan.

Zwitterionic polymer brush-functionalized dual-crosslinked hydrogel via subsurface-initiated atom transfer radical polymerization for anti-fouling applications

In this work, a double-network (DN) hydrogel embedded with initiator (HHyBr) was prepared and then reinforced with Fe3+, after which the antifouling polymer (poly-sulfobetaine vinyl imidazole, PSBVI) was successfully grafted onto the HHy-Br substrate via subsurface-initiated atom transfer radical polymerization (sSI-ATRP) to obtain PSBVI-functionalized HHy-Br (HHy-PSBVI). It has been demonstrated that Fe3+ can increase the crosslinking density of HHyBr; as a result, the tensile strength of the as-prepared hydrogel was increased from 0.07 to 1.75 MPa, as well as its anti-swelling properties were improved (from 648 % to 219 %). In addition, the presence of Fe3+ can efficiently promote the sSI-ATRP process. Compared with HHyBr, the PSBVI-functionalized HHy-Br manifested a significant decrease in friction coefficient, as the grafted high-thickness PSBVI brush (about 200 μm) has excellent hydrophilicity and can form a strong hydration layer for lubrication. Furthermore, the as-prepared HHy-PSBVI also exhibits outstanding antibacterial properties against E. coli (nearly 100 % were removed) and S. aureus (99 % were removed), as well as a satisfactory antifouling effect against microalgae (93 % Dunaliella and 92 % Chlorella were removed), which is attributed to the synergistic effect between the strong hydration of the zwitterionic polymer and the good bactericidal effect of the cationic imidazole group.

Morphology modified by polyvinylpyrrolidone for enhanced antibacterial and catalytic execution of bioactive Ag/ZnO composites based on hydroxyapatite in the synthesis of O-Aminocarbonitriles

Antibacterial resistance is growing in the world and is becoming a global threat to public health. In this project, we have checked the particular role of polyvinylpyrrolidone in the fabrication of nanospherical bioactive Ag/ZnO composite based on hydroxyapatite in a novel smart approach. Furthermore, the size and morphology controlled by the designed bioactive nanocomposite depend on the amounts of polyvinylpyrrolidone and this approach was investigated antibacterial activities, for the first time. Experimental data reveal that the best performance of antibacterial activity was increased only when the spherical morphology was formed (using the 4:3 M ratio between Zn2+ and Ag + ions in the presence of 0.5 polyvinylpyrrolidone, S5 sample). The results of disk-diffusion demonstrate that the highest growth inhibition zone was related to Staphylococcus aureus, which was increased approximately 18 mm compared to other bacteria (Escherichia coli, pseudomonas, aeruginosa, klebsiella pneumoniae). However, the efficient morphology (spherical morphology) was not observed in the presence of cetyltrimethyl ammonium bromide or other amounts of polyvinylpyrrolidone (S1–S4 samples have agglomerated-shaped). On the other hand, we endeavored toward applying designed nanostructure as a robust catalyst for the preparation of O-Aminocarbonitriles. The O-Aminocarbonitrile scaffolds were fabricated in great yield (up to 97%) under ambient conditions in the presence of designed nanocomposite. Consequently, the dual-purpose Ag/ZnO/HAp spherical nanocomposite with outstanding antibacterial and catalytic properties could be applied as promising antibacterial agents to prevent and treat implant infections and chemical reaction catalysts.