Otebo Bean Research Articles

Registration of ‘Samurai’ Otebo Bean

‘Samurai’ otebo bean (Phaseolus vulgaris L.) (Reg. No. CV‐312, PI 676022), developed by Michigan State University AgBioResearch, was released in 2015 as an upright, full‐season cultivar with virus (caused by Bean common mosaic virus) resistance and high yield potential. Samurai was developed using pedigree breeding method to the F6 generation followed by pure line selection for disease, agronomic, and quality traits. In 3 yr of field trials, Samurai yielded 2828 kg ha−1, flowered in 45 d, and matured in 100 d on average. Plants averaged 52 cm in height, with lodging resistance score of 1.6 and seed weight of 28.1 g 100 seed−1. Samurai combines high yield potential with erect architecture with full‐season maturity in an otebo seed type. Samurai has resistance to lodging and high pod placement within plant structure, making it suitable for direct harvest under narrow‐row production systems. Current otebo cultivars are short‐statured plants with determinate growth habit that require traditional pulling equipment due to the excessive seed loss that would result from direct harvest. Samurai is susceptible to race 73 of anthracnose [caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.‐Scrib], susceptible to rust race 22:2 [caused by Uromyces appendiculatus (Pers.:Pers.) Unger], and hypersensitive resistant to strain NL 3 of Bean common mosaic necrosis virus. Samurai otebo bean seed differs from other white‐seeded navy and great northern cultivars in possessing low levels of resistant starch, which makes it better suited for paste rather than canned bean products that meet industry standards.

Response of dry beans to halosulfuron applied postemergence

Soltani, N., Shropshire, C. and Sikkema, P. H. 2012. Response of dry beans to halosulfuron applied postemergence. Can. J. Plant Sci. 92: 723–728. Four field trials were conducted over a 2-yr period (2009 and 2010) at Exeter and Ridgetown, Ontario, to evaluate the tolerance of adzuki, black, cranberry, kidney, otebo, pinto, Small Red Mexican and white beans to halosulfuron applied postemergence (POST) at 35 and 70 g a.i. ha−1. All treatments including the non-treated control were maintained weed free during the growing season. Halosulfuron applied POST caused as much as 73, 7, 13, 12, 12, 11, 11 and 9% injury in adzuki, black, cranberry, kidney, otebo, pinto, Small Red Mexican (SRM) and white beans, respectively. Halosulfuron applied POST reduced adzuki bean height as much as 52 and 70% at Exeter and Ridgetown, respectively. Plant height was not affected in the other market classes of dry bean evaluated. Halosulfuron POST reduced shoot dry weight of adzuki bean 68% at both rates evaluated. Otebo and SRM bean shoot dry weight were not affected when halosulfuron was applied POST at 35 g a.i. ha−1 but otebo bean shoot dry weight was reduced 12% and SRM bean shoot dry weight was reduced 14% at 70 g a.i. ha−1. Shoot dry weight of black, cranberry, kidney, pinto and white bean was not affected with either rate of halosulfuron. Seed yield of adzuki bean was decreased 58% at 35 g a.i. ha−1 and 68% at 70 g a.i. ha−1 with halosulfuron. White bean yield was not affected with halosulfuron applied POST at 35 g a.i. ha−1 but was reduced 9% at 70 g a.i. ha−1. Seed yield of black, cranberry, kidney, otebo, pinto and SRM bean was not reduced with either rate of halosulfuron. Based on these results, there is not an adequate margin of crop safety for halosulfuron POST in adzuki bean. However, there is potential for POST application of halosulfuron in black, cranberry, kidney, otebo, pinto, SRM and white beans.

Response of Eight Market Classes of Dry Bean (<i>Phaseolus vulgaris</i> L.) to Pendimethalin

There is little information on the tolerance of dry bean to pendimethalin. Field studies were conducted in 2007 to 2009 at Exeter, Ontario and in 2008 and 2009 at Ridgetown, Ontario to evaluate tolerance of black, cranberry, kidney, otebo, pink, pinto, Small Red Mexican and white bean to the pendimethalin applied preplant incorporated at 1080 and 2160 g.a.i.ha-1. Pendimethalin PPI caused minimal injury in most market classes of dry bean at 1 and 2 WAE. There was no injury in various market classes of dry bean with the low dose at 1 and 2 weeks after emergence (WAE). However, at the high dose there was 0 to 4% injury at 1 WAE and 0 to 7% injury at 2 WAE in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Pendimethalin PPI was more injurious in white bean than in black, cranberry, kidney, otebo, pink, pinto and SRM bean. Pink and SRM bean exhibited the most tolerance to pendimethalin applied PPI at 1080 g.ai.ha-1 or 2160 g.ai.ha-1. Pendimethalin caused no adverse effect on plant height, shoot dry weight, seed moisture content and seed yield of black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Based on these results, there is an adequate margin of crop safety for pendimethalin applied PPI at the proposed dose of 1080 g.ai.ha-1 in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean in Ontario.

Effect of Halosulfuron Applied Preplant Incorporated, Preemergence, and Postemergence on Dry Bean

Five field trials were conducted over a 2-yr period (2007, 2008) at various locations in Ontario to evaluate the tolerance of black, cranberry, kidney, otebo, pink, pinto, small red Mexican (SRM), and white bean to halosulfuron applied PPI, PRE, and POST at 35 and 70 g ai/ha. There was minimal injury (3% or less) with halosulfuron applied PPI or PRE in dry bean. At Exeter and Ridgetown, halosulfuron applied POST at 35 and 70 g/ha caused 3 to 5% and 4 to 8% injury in dry bean, respectively at 1 wk after herbicide application (WAA). The injury was transient with no significant injury at 2 and 4 WAA. At Harrow, halosulfuron POST at 35 and 70 g/ha caused as much as 4% injury at 35 g/ha and 14% injury at 70 g/ha in dry bean. Halosulfuron applied PPI, PRE, and POST at 35 and 70 g/ha caused no decrease in plant height of dry bean except for kidney bean, which was reduced 6% at 70 g/ha, and white bean, which was reduced 3% at both 35 and 70 g/ha. Halosulfuron applied PPI, PRE, and POST at 35 and 70 g/ha caused no decrease in dry bean yield except for kidney bean, which was reduced 9% at 35 g/ha and 10% at 70 g/ha; otebo bean, which was reduced 3% at 70 g/ha; and white bean, which was reduced 7% at both 35 and 70 g/ha. On the basis of these results, there is an adequate margin of crop safety in dry bean to halosulfuron applied PPI or PRE at 35 and 70 g/ha. In addition, there is an adequate margin of crop safety in black, cranberry, pink, pinto, and SRM bean to halosulfuron applied POST at 35 and 70 g/ha. However, further research is required to ascertain the tolerance of kidney, otebo, and white bean to halosulfuron applied POST.

Registration of ‘Fuji’ Otebo Bean

‘Fuji’ Otebo bean (Phaseolus vulgaris L.) (Reg. No. CV‐289, PI 656392), developed by the Michigan Agricultural Experiment Station, was released in 2008 as a new early‐season, virus‐resistant cultivar. Fuji was developed as a fourth backcross line from the commercial cultivar Hime. Fuji differs from Hime in possessing resistance to Bean common mosaic virus. In comparative trials, Fuji is similar to Hime in performance (2406 kg ha−1), plant height (43 cm), lodging resistance (2.2), and seed size (27.6 g 100 seed−1). Fuji flowers in 43 d and matures in 90 d 3 d earlier than Hime. Fuji and Hime possess the same determinate growth habit and resistance to race 73 of anthracnose [caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.‐Scrib.]. Fuji meets the quality characteristics for use in sweet bean paste.

Dry Bean Response to Preemergence-Applied KIH-485

Three field trials were conducted over a 2-yr period (2004 and 2005) at Exeter and Ridgetown, Ontario to evaluate the tolerance of eight market classes of dry beans to KIH-485 applied PRE at 210 and 420 g ai/ha. KIH-485 PRE caused as much as 67% visual injury in small-seeded and 44% visual injury in large-seeded dry beans. KIH-485 applied PRE at 420 g/ha reduced plant height up to 47% at Ridgetown and 8% at Exeter in 2004, and reduced height of brown and white bean by 15 and 19%, respectively, but had no effect on the height of the other beans in 2005. Shoot dry weight was not affected at Exeter in 2004 but was reduced by 46% at Ridgetown in 2004 and 14% at Exeter in 2005. In 2004, seed moisture content increased by 5, 6, and 12% in black, otebo, and pinto beans, respectively. Seed yield was reduced up to 27% at Ridgetown and 11% at Exeter in 2004 but was not affected at Exeter in 2005. On the basis of this research, KIH-485 PRE causes unacceptable injury in some dry bean market classes.

Tolerance of Otebo Bean (Phaseolus vulgaris) to New Herbicides in Ontario

Weed management is a major production issue facing otebo bean growers in Ontario. Field trials were conducted at six Ontario locations during a 2-yr period (2003 and 2004) to evaluate the tolerance of otebo bean to the preplant incorporated (PPI) application of EPTC at 4,400 and 8,800 g ai/ha, trifluralin at 1,155 and 2,310 g ai/ha, dimethenamid at 1,250 and 2,500 g ai/ha,S-metolachlor at 1,600 and 3,200 g ai/ha, and imazethapyr at 75 and 150 g ai/ha. EPTC, trifluralin, dimethenamid, andS-metolachlor applied PPI resulted in minimal (less than 5%) visual injury and with exception of the low rate of dimethenamid causing a 16% reduction in shoot dry weight and the high rate causing an 8% plant height reduction had no adverse effect on plant height, shoot dry weight, seed moisture content, and yield. Imazethapyr applied PPI caused up to 7% visual injury and reduced plant height, shoot dry weight, and yield 8, 18, and 12% at 75 g/ha and 19, 38, and 27% at 150 g/ ha, respectively. Seed moisture content was also reduced by 0.4% with both rates. Based on these results, otebo bean is not tolerant of imazethapyr applied PPI at rates as low as 75 g/ha, the proposed use rate. EPTC, trifluralin, dimethenamid, andS-metolachlor applied PPI have a 2× rate crop safety margin for use in otebo bean weed management.

Responses of otebo bean (Phaseolus vulgaris L.) to postemergence herbicides

Ontario otebo bean growers have few herbicide options available for weed management. Six field trials were conducted in Ontario, Canada, over a 2 year period (2003 and 2004) to evaluate the tolerance of otebo bean to the postemergence (POST) application of bentazon at 1080 and 2160 g ai ha−1, fomesafen at 240 and 480 g ai ha−1, sethoxydim at 500 and 1000 g ai ha−1, quizalofop‐p‐ethyl at 72 and 144 g ai ha−1, imazamox plus fomesafen at 25 + 200 and 50 + 400 g ai ha−1, and imazamox plus bentazon at 25 + 600 and 50 + 1200 g ai ha−1. All treatments, including the untreated control, were maintained weed‐free during the growing season. The POST application of bentazon, imazamox plus fomesafen, and imazamox plus bentazon caused as much as 9% visual injury and reduced the plant height ≤ 12%, reduced the shoot dry weight ≤ 32%, and delayed maturity but had no adverse effect on the yield of otebo bean. Fomesafen, sethoxydim, and quizalofop‐p‐ethyl applied POST caused as much as 8% visual injury but this was transient and had no adverse effect on the plant height, shoot dry weight, seed moisture content, and yield of otebo bean, except for quizalofop‐p‐ethyl, which reduced the shoot dry weight as much as 18%. Based on these results, bentazon, fomesafen, sethoxydim, quizalofop‐p‐ethyl, imazamox plus fomesafen, and imazamox plus bentazon applied POST have an adequate margin of crop safety for weed management in otebo bean production in Ontario. However, care must be taken to avoid spray overlaps to prevent injury from bentazon, imazamox plus fomesafen, and imazamox plus bentazon.

Responses of Various Market Classes of Dry Beans (Phaseolus vulgarisL.) to Linuron

Tolerance of eight market classes of dry beans (black, brown, cranberry, kidney, otebo, pinto, white, and yellow eye beans) to the PRE application of linuron at the rate of 2.25 and 4.50 kg ai/ha was studied at two locations in Ontario, Canada, in 2003 and 2004. The eight market classes differed in their response to linuron. Linuron PRE caused as much as 43, 20, 7, 17, 54, 36, 56, and 12% visual injury in black, brown, cranberry, kidney, otebo, pinto, white, and yellow eye beans, respectively. Linuron PRE at 2.25 kg/ha reduced plant height 38% in otebo beans and 31% in white beans. Linuron PRE at 4.50 kg/ha reduced plant height 24 to 56% in black, brown, otebo, pinto, and white beans. Shoot dry weight was reduced in otebo beans by 56% and in white beans, by 46% at the low rate. Shoot dry weight was decreased 26 to 92% in black, otebo, pinto, white, and yellow eye beans at the high rate. There were no differences in the shoot dry weight of the other market classes. Linuron PRE at the low rate reduced otebo bean yield 42% and at the high rate reduced yields by 56, 74, and 61% in black, otebo, and white beans, respectively. There was no effect on the yield of other market classes. Differences in dry bean market class tolerance to linuron exists and may be summarized for these cultivars as cranberry > kidney > brown > yellow eye > pinto > black > white > otebo. Additional research is needed to determine if cultivars within a dry bean market class differ in their response to linuron.

Low root zone temperature effects on bean (Phaseolus vulgaris L.) plants inoculated with Rhizobium leguminosarum bv. phaseoli pre-incubated with methyl jasmonate and/or genistein

Methyl jasmonate (MeJA) has recently been shown to act as a plant-to-bacteria signal. We tested the hypothesis that pre-induction of Rhizobium leguminosarum bv. phaseoli cells with genistein and/or MeJA would at least partially overcome the negative effects of low root zone temperature (RZT) on bean nodulation, nitrogen fixation and plant growth. Otebo bean plants were grown at constant air temperature (25oC) and two RZT regimes (25 and 17oC) and inoculated with R. leguminosarum bv. phaseoli pre-induced with MeJA and/or genistein. Our results indicate that low RZT inhibited nodulation, nitrogen fixation and plant growth. The plants growing at low RZT began fixing nitrogen seven days later compared to those at higher RZT. The low RZT plants had fewer nodules, lower nodule weight, less N fixation, slower plant growth, fewer leaves, smaller leaf area, and less dry matter accumulation comared to plants at a higher RZT. Rhzobium leguminosarum bv. phaseoli cells induced with genistein and/or MeJA enhanced bean nodulation, nitrogen fixation and growth at both optimum and suboptimum RZTs. The results of this study indicate that MeJA improves bean nitrogen fixation and growth at both optimum and suboptimum RZTs, and can be used alone or in combination with genistein to partially overcome the low RZT induced inhibitory effects on nodulation and nitrogen fixation.

Otebo bean ( Phaseolus vulgaris) sensitivity to pre-emergence herbicides

Field trials were conducted at two Ontario locations in 2003 and 2004 to evaluate the tolerance of otebo beans to the pre-emergence (PRE) applications of dimethenamid (1250 and 2500 g ai/ha), S-metolachlor (1600 and 3200 g ai/ha), clomazone (1000 and 2000 g ai/ha), and imazethapyr (75 and 150 g ai/ha). Dimethenamid and S-metolachlor caused 5% or less visual injury at 7, 14, and 28 days after treatment (DAT). However, this injury was transient with no adverse effect on plant height, shoot dry weight, seed moisture content and yield of otebo beans. Clomazone caused greater visual injury at 7, 14, and 28 DAT and reduced plant height by 10% and shoot dry weight by 25% compared to the non-treated check, though yield was not reduced. Imazethapyr caused visual injury, at 7, 14, and 28 DAT and the injury was persistent over time resulting in a decrease of up to 42% in plant height, shoot dry weight, and yield of otebo beans, respectively. Based on these results, dimethenamid and S-metolachlor applied PRE have potential for weed management in otebo beans. Additional research is needed to determine if there is an adequate margin of crop safety in otebo beans to the PRE application of clomazone. However, otebo bean did not possess an adequate margin of crop safety for imazethapyr at the doses evaluated.