Ornamental Varieties Research Articles

The candidate gene SibHLHA regulates anthocyanin-driven purple pigmentation in Sesamum indicum flowers.

Anthocyanins not only serve as critical pigments determining floral hues but also play essential roles in attracting insects for pollination, feeding animals and mitigating abiotic stress. However, the molecular mechanisms underlying the regulation of flower color in sesame has not yetbeen reported. In this study, an F2 population was constructed by crossing 'Ganzhi 9' (purple-flowered) with 'BS377' (white-flowered). Genetic analysis revealed that purple flower is controlled by a single locus named as SiFC (Sesamum indicum flower color). Using the BSA-seq approach, SiFC was preliminarily identified on chromosome 6, which was further mapped to a 473kb interval using Kompetitive Allele Specific PCR (KASP) marker analysis. Moreover, functional annotation, expression profiling, and sequence analyses confirmed that the SibHLHA (Sesame10992) was the most likely candidate gene for SiFC. In addition, SibHLHA, highly homologous to AtTT8 (a key regulator in the anthocyanin synthesis pathway), was found to interact with WER-like or TTG1 proteins, enhancing anthocyanin accumulation in tobacco leaves. Furthermore, an SNP in the second exon of Sibhlha (BS377 variant)was found to alter the encoding amino acids, which affected Sibhlha binding to MYB protein and showed low anthocyanin in tobacco leaves compared with SibHLHA binding with WER-like or TTG1 proteins. These findings not only deepen our understanding of the molecular mechanisms controlling sesame corolla color, but also provide valuable insights for developing ornamental and consumable sesame varieties.

Transcriptome analysis reveals potential medicinal ingredient synthesis in ornamental Dendrobium.

Dendrobium is divided into ornamental and medicinal varieties due to ornamental and medicinal values. However, current research mainly focuses on medicinal Dendrobium, with less study on the medicinal value of ornamental Dendrobium. We analyzed the microstructures, active components of the stems from twelve ornamental Dendrobium, and explored the biosynthetic networks of these active components based on transcriptome sequencing. This study found the Dendrobium with the highest content of polysaccharide, alkaloid, and flavonoid was Dendrobium aphyllum (53.89 %), Dendrobium thyrsiflorum (2.11 %) and Dendrobium loddigesii (7.21 %). Further research revealed 9 DEGs associated with polysaccharide biosynthesis were highly expressed in D. aphyllum; 4 DEGs related to alkaloid biosynthesis were highly expressed in D. thyrsiflorum; 8 DEGs associated with flavonoid biosynthesis were highly expressed in D. loddigesii. This study revealed the potential medicinal value of ornamental Dendrobium and the synthetic mechanisms of its medicinal components, providing a foundation for the medical applications of ornamental Dendrobium.

Pré-melhoramento de um acesso de Capsicum annuum para uso ornamental

ABSTRACT Capsicum annuum is a domesticated pepper species that exhibits a wide range of fruit size, color, and shape, as well as plant characteristics important for ornamental pepper cultivars, including plant architecture and leaf size and color. This variability can be found in Capsicum accessions from germplasm banks and used in breeding programs to develop new ornamental pepper cultivars. The objective of this study was to develop the ornamental characterization and pre-breeding of a segregating Capsicum annuum accession from the Active Germplasm Bank of Capsicum of the Brazilian Agricultural Research Corporation (Embrapa Temperate Climate). Sixty-seven F1-generation and sixty-two F2-generation plants were characterized based on 16 morphological descriptors: stem color, nodal anthocyanin, plant growth habit, branching density, leaf density, leaf color, number of leaves per axil, flower position, corolla color, calix pigment, immature fruit color, fruit position, mature fruit color, fruit shape, fruit apex shape, and fruit brightness. The relative contribution of each trait to the genetic divergence was determined using the method proposed by Singh (1981). A principal component analysis was carried out. Almost all evaluated variables showed segregation, except for flower position and immature fruit color. Genetic variability was found in both F1 and F2 generations, indicating their potential for developing ornamental Capsicum annuum varieties.

The Existence of Indonesian Jewelry: Modern and Contemporary Styles based on the Intuition of the Creation of Traditional Ideas

This article explores the significance of the jewelry-making tradition in Indonesia and its evolution over time, based on the unique techniques, materials, designs, and varieties of ornaments that make Indonesian jewelry famous locally and internationally. This article aims to analyze jewelry that can have an economic impact based on the local community's culture and its potential for sustainable development in the region. The research method used was qualitative descriptive, and it was in Jepara, Jombang, Tulungagung, Mojokerto, Ciamis, and Garut in Indonesia, with 30 respondents (artisan groups). Data collection will be done through observation and in-depth interviews to analyze jewelry designs and ornamental varieties—data analysis will be done using the source triangulation technique. The study results show the symbolism and cultural significance of Indonesian jewelry design sourced from traditional values. The source of creation is from the surrounding nature, in the form of plants, streams, branches, and leaves that develop in Indonesia. By combining traditional craftsmanship and ideas with modern marketing and distribution channels, jewelry in Indonesia has survived to this day.

A Key R2R3-MYB Transcription Factor Activates Anthocyanin Biosynthesis and Leads to Leaf Reddening in Poplar Mutants.

Colorful woody plants are highly valued for their ornamental qualities, and are commonly used in garden landscape design. We previously cultivated several ornamental poplar varieties from bud mutants of Populus sp. Linn. '2025' (ZL2025), each with different leaf colors. Based on transcriptome data from these varieties with varying anthocyanin pigmentation, we identified and named an R2R3-MYB gene, PdMYB113. The mRNA of PdMYB113 accumulated in the leaves of the red-leaf mutants 'QHY' and 'LHY', but barely expressed in the leaves of 'ZL2025'. The anthocyanin biosynthesis genes were upregulated, resulting in high levels of red anthocyanins (particularly Peonidin-3-O-rutinoside, Cyanidin-3-O-rutinoside, and Cyanidin-3-O-glucoside) in both OE-PdMYB113 tobacco and poplar plants. This upregulation caused a color change in the tissues from green to red or dark purple. Yeast one-hybrid and luciferase assays demonstrated that PdMYB113 activates the expression of anthocyanin biosynthesis genes, including the early anthocyanin biosynthetic gene PdCHS and the late anthocynin biosynthetic gene PdANS. Consequently, PdMYB113 is identified as a key regulator of red coloration in poplar. Additionally, PdMYB113 does not dwarf transgenic plants under normal lighting conditions. This study elucidates the regulatory mechanisms of color change in ZL2025 and highlights a crucial gene for breeding new varieties of woody plants.

Tissue Culture-Independent Approaches to Revolutionizing Plant Transformation and Gene Editing

Abstract Despite the transformative power of gene editing for crop improvement, its widespread application across species and varieties is limited by the transformation bottleneck that exists for many crops. The genetic transformation of plants is hindered by a general reliance on in vitro regeneration through plant tissue culture. Tissue culture requires empirically-determined conditions and aseptic techniques, and cannot easily be translated to recalcitrant species and genotypes. Both Agrobacterium-mediated and alternative transformation protocols are limited by a dependency on in vitro regeneration, which also limits their use by non-experts and hinders research into non-model species such as those of possible novel biopharmaceutical or nutraceutical use, as well as novel ornamental varieties. Hence, there is significant interest in developing tissue culture-independent plant transformation and gene editing approaches which can circumvent the bottlenecks associated with in vitro plant regeneration recalcitrance. Compared to tissue culture-based transformations, tissue culture-independent approaches offer advantages such as avoidance of somaclonal variation effects, with more streamlined and expeditious methodological processes. The ease of use, dependability, and accessibility of tissue culture-independent procedures can make them attractive to non-experts, outperforming classic tissue culture-dependent systems. This review explores the diversity of tissue culture-independent transformation approaches and compares them to traditional tissue culture-dependent transformation strategies. We highlight their simplicity and provide examples of recent successful transformations accomplished using these systems. Our review also addresses current limitations and explores future perspectives, highlighting the significance of these techniques for advancing plant research and crop improvement.

Variety Of Bhenga And Manu Ornaments At The Sa'o Traditional House Of Gurusina Traditional Village

East Nusa Tenggara (NTT) has a rich culture and diverse traditions. Several ethnic sub-groups, including the Ngada-Lio ethnic sub-group, mark this diversity. This ethnic sub-group inhabits the Ngada Regency area, where traditional villages are scattered at several points. Traditional villages are places to preserve a culture that has been passed down from generation to generation, one of which is weti carvings. These carvings have a variety of figures, including Bhénga and Manu. The depiction of these two figures is like a buffalo horn and a chicken. In some traditional houses in Indonesia, there are also similar figures, namely in the Tongkonan Toraja traditional house and the Karo Batak traditional house. This research is a qualitative descriptive research. The researcher chose this method to describe the figures of Bhéngaand Manu in detail and depth. This research began with observation and interviews, after which the research continued by analyzing the data using morphological, stylistic, and comparative analysis. The results of this study show that various ornamental figures of buffalo and chicken are depicted on the walls of the Sao traditional house because the two animals have a special closeness to the community. Besides that, the ornamental variety is carved on the wall of the traditional house as an addition to the aesthetic value of the traditional house.

Morphological Diversity of F1 Ornamental Chili Generation from Single Cross Hybridization

Ornamental chilli (Capsicum annuum L.) belongs to the Solanaceae family and is highly popular for its aesthetic value. Ornamental chilli plants are edible, with attractive plant architecture and fruit shapes, as well as varying levels of spiciness. The most preferred traits in ornamental chilli plants include compact size (suitable for potted plants), diverse and unique fruit shapes and colors, and upright fruit orientation. This study aims to investigate the morphological diversity in the F1 generation resulting from a single cross-hybridization between two ornamental chilli varieties, namely the Medusa Pepper, known for its compact growth habit, and the Black Pearl Pepper, characterized by purple-coloured fruits and leaves. Analysis of the qualitative data shows that the F1 generation shares a high degree of morphological similarity with the female parent, Black Pearl Pepper, with a similarity value exceeding 75%, while it exhibits no significant resemblance to the male parent, Medusa Pepper, with a similarity value of 0%. The F1 generation from the single cross demonstrates limited morphological variation, with all observed traits being largely influenced by dominant characteristics, including fruit color, plant habit, and leaf color.

Refleksi Budaya Etnis Tionghoa dalam Kesenian Barongsai di Kota Singkawang

The lion dance is a form of ethnic Chinese culture with a beautiful variety of motion and visual ornaments. The purpose of this research is to serve as a reminder of cultural preservation and inheritance. The primary reflection resources paired with Feldman's art criticism method include a historical overview, performance dynamics, ritual/celebration procedures, and cultural heritage. This study also provides a glimpse into and description of ethnic Chinese's effort to express cultural arts following a time of prejudice. The dissection is carried out utilising descriptive qualitative methodologies, with data collected through participant observation, in-depth interviews, and documentation. Triangulation of sources with ethnomusicologists validates the data analysed using an inter-academic model. Cultural reflection produced by Barongsai arts for ethnic Chinese includes reminders and awareness of evaluative materials related to the struggle for discrimination in the historical context, entertainment and sports functions in the context of performance dynamics, meanings and symbols of happy and sad expressions in the context of ritual / celebration processes, and sorting out incoming outside cultures for the younger generation in the context of cultura. It is critical and beneficial that the findings of this study serve as a vehicle and platform for future attempts to maintain and enhance the Barongsai art form. In addition, there needs to be support between elements of the ethnic Chinese community and the government so that the art of Barongsai can be preserved and developed in the future.

Ornamental Pothos – A Taxonomic Dilemma

As an indoor plant and air purifier, ornamental Pothos are becoming increasingly popular. This group of evergreen plants is usually grown as a hanging houseplant and has thick, heart-shaped, green, waxy leaves with traces of yellow. There is disagreement on the taxonomic identity of ornamental Pothos varieties. Thus, a study was carried out using 21 ornamental Pothos gathered from various nurseries. One species of the genus Pothos, P. scandens, is used as a check. The ornamental Pothos varieties are classified into 11 species across 5 genera, which include 3 species of Monstera, 3 species of Philodendron, 2 species of Raphidophora, 2 species of Scindapsus, and 1 species of Epipremnum. None of them are members of the genus Pothos, a group of flowering plants in the monocotyledonous family Araceae, despite sharing the common name Pothos. Both true and ornamental Pothos have therapeutic benefits as well, including anti-oxidant, anti-bacterial, anti-fungal, and anti-carcinogenic properties. A brief, in-depth description and colour images are included for simple identification of the species.

Light Quality Influence on Growth Performance and Physiological Activity of Coleus Cultivars

This study investigates the influence of different light qualities, including red, green, blue, purple, and white lights, on the growth, physiological activity, and ornamental characteristics of two Coleus cultivars. Emphasizing the importance of leveraging phenotypic plasticity in plants within controlled environments, using light quality is a practice prevalent in the ornamental industry. The research explores the varied responses of two Coleus cultivars to distinct light spectra. The key findings reveal the efficacy of red light in enhancing shoot and leaf parameters in C. ‘Highway Ruby’, while red and green light exhibit comparable effects on shoot width and leaf dimensions in C. ‘Wizard Jade’. White light-emitting diodes (LEDs), particularly with color temperatures of 4100 K and 6500 K, promote root length growth in the respective cultivars. Moreover, chlorophyll content and remote sensing vegetation indices, including chlorophyll content (SPAD units), the normalized difference vegetation index (NDVI), the modified chlorophyll absorption ratio index (MCARI), and the photochemical reflectance index (PRI), along with the chlorophyll fluorescence, were significantly affected by light qualities, with distinct responses observed between the cultivars. In summary, this study highlights the transformative potential of LED technology in optimizing the growth and ornamental quality of foliage plants like Coleus, setting a benchmark for light quality conditions. By leveraging LED technology, producers and nursery growers access enhanced energy efficiency and unparalleled versatility, paving the way for significant advancements in plant growth, color intensity, and two-tone variations. This presents a distinct advantage over conventional production methods, offering a more sustainable and economically viable approach for increased plant reproduction and growth development. Likewise, the specific benefits derived from this study provide invaluable insights, enabling growers to strategically develop ornamental varieties that thrive under optimized light conditions and exhibit heightened visual appeal and market desirability.

Comparative Transcriptome Analysis Reveals Differential Cutaneous Gene Expression in the Color Variation of Two Ornamental Discus, Red Melon and Red Cover.

Red Melon (RM) and Red Cover (RC) discus (Symphysodon spp.) are ornamental fish varieties that were selectively bred from the wild parental lineages of the brown discus S. aquafaciatus over many generations, resulting in distinct cutaneous patterns from juveniles to adults. To better understand the underlying mechanisms, skin samples were collected from juveniles aged 60 days and adults aged 1 year from RM and RC for investigations. Microscopic observation detected xanthophores and erythrophores in all samples, except RC juveniles with no erythrophores. Melanophores were presented only in RC. The comparative analysis revealed that genes involved in pteridine synthesis (gch1 and zgc:153031), one-carbon metabolism (aldh1l2 and zgc153031), and lipid metabolism (apoda and klf1) were differentially expressed in RM juveniles, which may be associated with the development of erythrophores and xanthophores. The temporal inhibition of melanophore differentiation and development was observed in RM juveniles, coupled with elevated expression of notum2 and sost, two antagonist genes in Wnt-signaling, suggesting their roles in melanophore development. Distinct pigment pattern between RM and RC since the juvenile stage may be driven by the differential expression of multiple axial developmental genes, including GATA, ankyrin, and mitotic spindle orientation proteins. This is the first report to describe the differential growth of cutaneous pigments and the molecular processes involved in red discus. The results provided valuable insights into pigment pattern differences in an interesting ornamental fish model.

Home sweet home: Evaluation of native versus exotic plants as resources for insects in urban green spaces

Abstract Insect decline and loss of biodiversity not only affect large‐scale agricultural landscapes, but are increasingly recognized in urban environments. It is undisputed that a greater supply of flowers in urban green spaces can provide insects with more food and habitat. However, it is still controversial whether native wild plants or non‐native ornamental plants and varieties are the right choice. To answer this question we investigated the number of insects interacting with different types of plants: twelve ornamental and six related wild perennials. In this context, the number of flower visitors per plant species and plot was recorded at 10‐minute observation intervals, as well as the feeding damage caused by insect herbivores on the leaves and stems of the study plants. We established 18 plant species in ten independent study plots in the city of Darmstadt, Germany. The plants were six native wild plant species, six ornamental plant species related to the wild plants from the same genus or family and six exotic ornamental plant species from other genera and families than the wild plants. Native insects (wild bees, flies, beetles, wasps) that feed on pollen and nectar visited wild perennials significantly more often (67% of all visits) than related ornamental (24%) and unrelated exotic plants (9%). In contrast, honey bees (Apis mellifera) showed no preferences to any of the three target plant groups and interacted with other plant species than most of the native insects in our study according to analyses of interaction networks. The assessment of leaf damage caused by insect herbivores on the individual plants confirmed a similar and significant difference in the insects' choices. Leaves from wild plants showed the highest herbivory (mean 2.3% of the leaf area), followed by related ornamental plants (0.8%), whereas unrelated exotic plants were hardly consumed (0.1%) by herbivores. Practical implication. Our study shows that in urban green spaces, both flower‐visiting and leaf‐feeding insects are more likely to use native wild plants as a food source than closely related and exotic ornamental plants.

Variety Breeding Studies on Hesperis isatidea (Boİss.) D.A. German & Al-Shehbaz

Hesperis isatidea (Boiss.) D.A.German & Al-Shehbaz, a member of the Brassicaceae family, represents a distinctive natural species characterized by its fragrant, conspicuous inflorescences, unique flower coloration, and distinct feather-like foliage. The Turkish name of the species is known as “Allı Gelin”. This species is native, exhibiting resilience to drought conditions and requiring minimal care. Cultivation studies on this species were carried out at Erzincan Horticulture Research Institute (Turkey) with project number 106G022 within the scope of TÜBİTAK (Turkish Scientific and Technological Research Council). The research aimed to determine optimal production techniques through generative and vegetative propagation methods. The project, initiated in 2013 and concluded in 2022, focused on developing a cultivar of the Allı Gelin plant using texel selection breeding techniques. Thirteen populations of Allı Gelin from institute's gene pool were utilized. Employing the Texel Selection method, four distinct lines were identified: compact, tightly spread compact, pyramid1and pyramid-2 shaped. Subsequent baby plant tests revealed stability in the tightly spread compact and pyramid-1 lines, leading to their selection for further breeding. Although breeding efforts continued on the compact line, the pyramid-2 line was discontinued due to an inability to attain the desired stability. The successfully tested variants, namely the pyramid-1 and tightly spread compact forms, have been earmarked as candidates for outdoor ornamental plant varieties. Registration procedures for these candidates are underway. These new varieties promise to enrich diversity within the ornamental plants sector, potentially enhancing competitiveness in foreign markets.

AESTHETICS OF PLAK-PLIENG GRAVESTONE OF LAMURI KINGDOM ACEH: A STUDY ACCORDING TO SUSANNE KNAUTH LANGER'S AESTHETIC CONCEPT

The Lamuri Kingdom in Aceh has relics in the form of gravestones called "Plak-plieng" stones, which are concrete evidence and records of the history of the kings who were buried with these gravestones. This Plak-plieng gravestone is thought to be a gravestone made by local people during the reign of the Lamuri kingdom with a mixed population who emigrated from outside Aceh at that time. The Lamuri Kingdom was an Acehnese Hindu kingdom that existed in the 4th century, turned into Islam or a sultanate in the 9th century, and lasted until the 15th century. The Plak-plieng gravestone, which is characteristic of the Lamuri royal graves, was rediscovered in 1890. Based on the date written on the gravestone found, the Plak-plieng gravestone dates to the 12th century AD. Thus, the Plak-plieng gravestone was made during the Islamic era, or when Lamuri became an Islamic kingdom. The type of research in this study is research using a descriptive method. The descriptive method is a method used to look for elements, characteristics, and properties of a phenomenon. The method begins with collecting data, analysing the data, and interpreting it. This descriptive research tries to reveal and explain the problems that occur in society, especially the problem of searching for symbolic aesthetics on the Plak-plieng gravestones of the Lamuri kingdom of Aceh. The Plak-plieng gravestone contains various symbolic elements that we can find using Susane K. Langer's theory. The findings of the symbolic aesthetic meaning found are divided into several parts according to Langer's aesthetic theory. On the Plak-plieng gravestones of the Lamuri Kingdom of Aceh, symbolic aesthetics are found as a symbol of art. The implied value of this gravestone gives us an idea of the high artistic value that existed in past societies. In this way, we can see that the level of artistic taste of the people of Aceh in the past was very high. The discovery of the inscription on this gravestone also explains the glory days of a kingdom led by the owner of the gravestone. This day dates back nine hundred years. In this way, we can learn about the value of art in the past through the Acehnese civilisation and how they found materials for making sturdy gravestones and a variety of ornaments that were very complicated and beautiful.

Ornamental Variety of Tombs of Sigam Kings in 1855-1900 in the Wetland Area of Sigam Sea Island, Kotabaru Regency

History and culture have begun to be fulfilled by the younger generation, it takes effort to map history and concrete explanatory evidence of each heritage site from the past. Kotabaru Regency is one of the centers of study of this research because many historical relics were found in the form of tombs from the Sigam Kingdom that received less attention from related agencies. Therefore, deeper research is needed so that it can be an evaluation material for future policies and strategies. This study aims to find out how the shape of the tomb (typology) and the variety of ornamental tombs of the kings of Sigam in 1855-1900. The research method uses historical methods: (1) heuristics, (2) source criticism, (3) interpretation, (4) historiography. The results of this study are as follows: This cemetery is a complex of tombs of kings, Kingdoms, Sea Island and their relatives. The King of Pulau Laut who is buried in this complex there are three people, namely Prince Jaya Sumitra bin Pangeran Muhammad Nafis, Prince Abdul Kadir bin Pangeran Muhammad Nafis, and Pangeran Brangta Kesuma bin Pangeran Abdul Kadir. The tombstones of the kings are currently covered in yellow cloth by the surrounding community.

KAJIAN KOMPOSISI WARNA BATIK GENTONGAN MADURA STUDI KASUS BATIK PRODUKSI “ZULPAH BATIK”

Batik Gentongan is one of the coastal batiks with diverse ornamental varieties and color compositions. Evolving through the crossing of various cultures, Madura batik has a dynamic, practical, egalitarian character, as well as a desire to be different with striking patterns and colors. The variety of colors in Batik Gentongan looks very bold, besides that the various colors are also obtained through a unique coloring process so it is very interesting to learn. This objective can be achieved by conducting a color composition study on batik produced by Zulpah Batik in Tanjungbumi. The color study was carried out with 30 pieces of batik which were grouped based on their basic colors, namely black, red, brownish red, blue, brown, and white. The colors studied will be grouped into 2 color groups, namely basic colors and decorative colors

Selection and validation of reference genes for quantitative real-time PCR in different tissues of Clematis lanuginosa

BackgroundThe lack of reference genes makes it difficult to conduct molecular biology research on the plant genus, Clematis L. Clematis lanuginosa belongs to Sect. Viticella DC of Clematis L. It is also an important ornamental cultivated variety parent of the early and late large-flowered groups. Studying the reference genes of C. lanuginosa in different tissues will provide a theoretical basis for the reference selection of early and late large-flowered groups of Clematis, which could promote research progress on molecular biology of ornamental Clematis. ResultsThe roots, stems, leaves, sepals, stamens, and carpels of C. lanuginosa were used as research materials, and seven candidate reference genes were used for quantitative real-time PCR analysis. Comprehensive stability analysis using geNorm, NormFinder, BestKeeper, and RefFinder software showed that suitable reference genes in C. lanuginosa root, stem, and leaf were PP2A-2 and UBC34; and in floral tissue were UBC34, PP2A-2, and ARP7. These reference genes can be used as internal reference either alone or in combination. The pairwise variation value evaluated with geNorm software showed that two internal reference genes were needed for gene expression correction in the tissues. In the floral organs, three reference genes were required for gene expression correction. ConclusionsOur results provide a foundation for future gene expression analysis of C. lanuginosa and guidance for the screening of reference genes in Clematis.How to cite: Li Q, Wang S, Lv F, et al. Selection and validation of reference genes for quantitative real-time PCR in different tissues of Clematis lanuginosa. Electron J Biotechnol 2024;70. https://doi.org/10.1016/j.ejbt.2024.04.005.

Сорт декоративного подсолнечника Гелия селекции ВНИИМК

In 2023, an ornamental sunflower variety Geliya was transferred to the State Commission of the Russian Federation on testing and protection of breeding achievements. It was developed by crossing lines LD 24 and LD 30, and consequent selfpollination under individual selection by traits of morphotype with transfer of a fertile line on CMS RIG. The originator of the ornamental sunflower variety Geliya is V.S. Pustovoit All-Russian Research Institute of Oil Crops.

Cytology of a seminoma in a koi (Cyprinus carpio): a rapid diagnostic tool

Koi(Cyprinus carpio) is an ornamental variety of common carp frequently kept as pets. Given their long lifespan, neoplasia, albeit uncommon, may occur in these animals, and only a few studies have faced their cytological diagnosis. In the present case, a koi carp was referred to the clinicians due to coelomic swelling. The carp underwent surgery, which revealed an enlargement of both testes. Testicular samples were cytologically and histologically examined. The lesion was diagnosed as a seminoma since it was composed of round, large, atypical, and often multinucleated cells with round central nuclei and moderate cytoplasm. These tumors had the same appearance as seminomas in mammals and should be considered among differential diagnoses when coelomic swelling occurs in koi carp. Seminomas in koi carp are diagnosed histologically, but cytology, a rapid and cheap exam executable in all veterinary clinical facilities, could be a relevant preliminary diagnostic tool that may influence the entire diagnostic process.