Original Substrate Research Articles

A new sample preparation protocol for SEM and TEM particulate matter analysis

A new methodology has been developed to prepare electron microscopy, both SEM and TEM, specimens starting from particulate matter collected using environmental sampling systems. The approach is based on the extraction of the particles to be analyzed from the harvesting substrates. The extracted particles can be directly observed in an SEM, possibly in low-vacuum mode to prevent electrical charging. In order to prepare electron transparent samples, TEM observations require a further step, consisting in embedding the particles in an electron transparent carbon film deposited before dissolving the acetate extracting substrate. The protocol has been tested by analyzing particles collected during bench tests on brake pads and discs, carried out on a dynamometer equipped with a particulate matter sampling apparatus. The main advantages of the approach are: the complete extraction of the particulate matter specimens from the original substrates, that in this way do not interfere with the analyses; the extracted samples retain the topological information of the collection in the specimens prepared for SEM; possibility to be applied to any kind of particulate matter harvesting substrates.

Noninvasive, label-free, and quantitative monitoring of lipase kinetics using terahertz emission technology.

Enzymes catalyze chemical transformations of great importance in many fields, and analysis of the rate of these transformations is equally important. The latter are typically monitored using surrogate substrates that produce quantifiable optical signals, owing to limitations associated with "label-free" techniques that could be used to monitor the transformation of original substrate molecules. In this study, terahertz (THz) emission technology is used as a noninvasive and label-free technique to monitor the kinetics of lipase-induced hydrolysis of several substrate molecules (including the complex substrate whole cow's milk) and horseradish peroxidase-catalyzed oxidation of o-phenylenediamine in the presence of H2 O2 . This technique was found to be quantitative, and kinetic parameters are compared to those obtained by proton NMR spectroscopy or UV/Vis spectroscopy. This study sets the stage for investigating THz emission technology as a tool for research and development involving enzymes, and for monitoring industrial processes in the food, cosmetic, detergent, pharmaceutical, and biodiesel sectors.

Secret messaging with endogenous chemistry

Data encoded in molecules offers opportunities for secret messaging and extreme information density. Here, we explore how the same chemical and physical dimensions used to encode molecular information can expose molecular messages to detection and manipulation. To address these vulnerabilities, we write data using an object’s pre-existing surface chemistry in ways that are indistinguishable from the original substrate. While it is simple to embed chemical information onto common objects (covers) using routine steganographic permutation, chemically embedded covers are found to be resistant to detection by sophisticated analytical tools. Using Turbo codes for efficient digital error correction, we demonstrate recovery of secret keys hidden in the pre-existing chemistry of American one dollar bills. These demonstrations highlight ways to improve security in other molecular domains, and show how the chemical fingerprints of common objects can be harnessed for data storage and communication.

Plant parasitic nematodes, substrates or grass species as reasons for poor reestablishment of a sand‐based putting green after winter kill

AbstractIn 2018–2019, establishment problems were encountered, after reseeding creeping bentgrass (Agrostis stolonifera) on a sand‐based putting green after ice encasement at the NIBIO Turfgrass Research Center, Norway. Seeds germinated, but the seedlings attained a purple color and died in large patches. Replacement of the top 3 cm layer with new sand amended with Sphagnum peat or garden compost did not solve the problem. To explain this phenomenon, we (1) analyzed the original substrate for nematodes in patches with and without reestablishment failure; and (2) conducted a factorial pot trial with creeping bentgrass and Chewings fescue (Festuca rubra ssp. commutata) seeded on different substrates, some of them in layers, and with and without phosphorus (P) fertilization. The nematode counts showed six times more stubby‐root nematodes and two times more spiral nematodes and needle nematodes in the patches with dead seedlings than in the patches with healthy seedings. In the pot trial, the fastest and slowest reestablishment was observed with new sand amended with garden compost and in the two treatments that included the original substrate, respectively. Replacement of the top 3 cm of the old substrate with new garden compost resulted in stagnation of bentgrass seedlings from four weeks after seeding, while fescue seedlings were unaffected. We conclude that the failure to reestablish creeping bentgrass was primarily due to nematodes, which are likely to be more critical for seedlings than for established turf. The green was later reestablished successfully with a 100 % red fescue seed blend.

Design and In silico Studies of 2,5-Disubstituted 1,2,4-Triazole and 1,3,4-Thiadiazole Derivatives as Pteridine Reductase 1 Inhibitors

Aims: Design and in silico studies of 2,5-disubstituted triazole and thiadiazole derivatives as Pteridine Reductase 1 inhibitors. With a view to develop effective agents against Leishmaniasis, 2-substituted-5-[(1H-benzimidazol-2yl) methyl] azole derivatives (A1-A12) were designed against the target enzyme Pteridine reductase 1.
 Methodology: The series was designed by targeting Pteridine reductase 1 which is an enzyme responsible for folate and pterin metabolism. Based on thorough study of the enzyme structure and structural features of ligands required for optimum interaction with the enzyme, a series of 12 compounds consisting of 2,5-disubstituted 1,2,4-triazole and 1,3,4-thiadiazole derivatives was designed. In silico studies were carried out which included docking studies (using V Life software) to understand binding of the compounds with enzyme PTR1, ADMET studies, drug likeness studies for physicochemical properties and bioactivity studies to understand the possible mechanism of action of the compounds. These studies were undertaken using online softwares, molinspiration and admetSAR web servers.
 Results: Compounds A10 and A12 gave the best docking scores of -59.9765 and -60.4373 respectively that were close to dihydrobiopterin (original substrate). All the compounds complied with Lipinski’s rule of five. Most of the compounds displayed favorable ADMET properties.
 Conclusion: The 2,5-disubstituted 1,2,4-triazole and 1,3,4-thiadiazole derivatives exhibited good binding affinity for PTR1 enzyme (PDB code: 1E92). The docking scores indicated that enzyme binding may be governed by the nature and size of the substituents on the azole ring. The compounds display well-defined drug-like and pharmacokinetic properties based on Lipinski’s rule of five with additional physicochemical and ADMET parameters. Bioactivity studies suggested the possible drug mechanism as enzyme inhibition. Hence, this study provides evidence for consideration of valuable ligands in 2,5-disubstituted 1,2,4-triazole and 1,3,4-thiadiazole derivatives as potential pteridine reductase 1 inhibitor and further in vitro and in vivo investigations may prove its therapeutic potential.

Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation.

Environmental pollution with alkylbenzene hydrocarbons such as toluene is a recurring phenomenon. Their toxicity and harmful effect on people and the environment drive the search for sustainable removal techniques such as bioremediation, which is based on the microbial metabolism of xenobiotic compounds. Melanized fungi present extremophilic characteristics, which allow their survival in inhospitable habitats such as those contaminated with hydrocarbons. Screening methodologies for testing the microbial assimilation of volatile organic compounds (VOC) are scarce despite their importance for the bioremediation of hydrocarbon associated areas. In this study, 200 strains of melanized fungi were isolated from four different hydrocarbon-related environments by using selective methods, and their biodiversity was assessed by molecular and ecological analyses. Seventeen genera and 27 species from three main orders, namely Chaetothyriales, Cladosporiales, and Pleosporales, were identified. The ecological analysis showed a particular species distribution according to their original substrate. The isolated strains were also screened for their toluene assimilation potential using a simple and inexpensive methodology based on miniaturized incubations under controlled atmospheres. The biomass produced by the 200 strains with toluene as the sole carbon source was compared against positive and negative controls, with glucose and with only mineral medium, respectively. Nineteen strains were selected as the most promising for further investigation on the biodegradation of alkylbenzenes.

Triclosan degradation in sludge anaerobic fermentation and its impact on hydrogen production

Triclosan (TCS), a widely used antibacterial agent, was accumulated at significant levels in waste activated sludge (WAS). To date, however, the interaction between TCS and sludge anaerobic fermentation was rarely reported. Hence, this work aimed to deeply understanding the degradation of TCS in sludge anaerobic fermentation and its impact on hydrogen production. Experimental results showed that ~ 45% of TCS was degraded in long-term anaerobic fermentation, with 2,4-dichlorophenol as its main intermediate. Based on the information from high performance liquid chromatography–mass spectrometry analysis, three pathways i.e., dechlorination, hydroxylation, and cleavage of ether bonds, were proposed for TCS degradation. It was found that the maximum hydrogen yield decreased from 18.6 to 12.8 mL/g VSS with the increase of TCS from 12 to 487 mg/kg TSS. One possible reason for the decreased hydrogen yield was that a part of hydrogen generated might serve as electron donors for TCS dechlorination. Besides, the presence of TCS significantly suppressed acidogenesis (an important step responsible for hydrogen generation). This inhibition to acidogenesis is likely due to that the high-affinity functional groups of TCS such as hydroxyl groups could bind to the active sites of acetate kinase (AK, a key enzyme in acidogenesis), which reduced the active sites available for original fermentation substrates. Microbial analysis revealed that TCS increased the relative abundances of potential contaminant decomposers such as Guggeheimella but inhibited the populations of hydrogen producers such as Proteiniborus, which was consistent with the results obtained by chemical analyses.

Electronically Reconfigurable HM-SIW Band-pass Filter Based on New CSRR Design Using PIN Diodes

In this paper, a novel and original reconfigurable half-mode substrate integrated waveguide (HM-SIW) band-pass filter (BPF) is proposed. The proposed BPF is composed of two different size new design complementary split-ring resonators to achieve the compact size, and two PIN diodes to achieve the reconfigurability. This filter can function in three different cases according to the ON/OFF combination states of the PIN diodes. The operating state can either be a dual-band-pass filter with resonant frequencies 2.5GHz and 3.6 GHz that have measured return loss (RL) less than -23 dB and -25 dB, respectively. Or it can operate as a single-band-pass filter in two other cases. The resonant frequency of the first is 2.6 GHz that has a measured RL of -20 dB, and for the second one, the resonant frequency is 3.35 GHz that has -35 dB as a measured RL. Moreover, the measured insertion loss (IL) is better than 1 dB for all the cases. The size of this filter design is 26.3mm x 12mm which makes it a very compact device considering that it functions in the S-band compared to publish work that targets the same frequency band.

The use of unconventional organic waste for vermicomposting in Surgut greenhouses

The paper discusses the use of non-traditional types of organic waste in vermicomposting in greenhouses on the territory of the middle taiga forest zone of Western Siberia. Experimental studies were carried out in the laboratories of the scientific and educational center and the center for collective use of the Institute of Natural and Technical Sciences of Surgut State University in 20012020. The main objects of the study were brewers grains, waste from the production of the Kristal brewing company in Surgut and wastewater sludge from the treatment facilities of the Gorvodokanal in Surgut. Additionally, the composition of complex composts included sand, office waste paper, waste coffee, food waste, leaf and grass litter, bird droppings and the microbiological additive Tamir. The compost worms of the Eisenia foetida Prospector species were used as vermiculture. 10 juveniles were launched in the first series of experiments and 50 juvenile worms were launched in the second series. The resulting vermicomposting is more effective in quality than the original substrates. The use of the hybrid Prospector can be effectively used in the disposal of non-traditional organic waste. Complex composts mature more efficiently when microbiological additives are added. In the studied substrates the content of heavy metals was reduced to MPC norms.

Lime-based Sacrificial Layers – Evaluation of a Traditional Conservation Method Applied in an Urban Environment

The use of modified lime slurry as a sacrificial layer to protect the original porous substrate has a long tradition in the practice of building and monument conservation in Austria. This paper presents the results of analyses performed on the Natural History Museum Vienna to get more insight into the long-term performance of this conservation method. Stone surfaces on the facade and roof area, covered with an acrylic tempered lime sacrificial layer and subsequently made water-repellent, were tested in situ and in the laboratory. Whilst coatings in the exposed zones were completely vanished in certain areas, the samples from the facade were in a good condition even after nearly twenty years of exposure. Hydrophobic activity could be verified up to a depth of five mm in the porous stone substrates. Despite the general good state of preservation of most surfaces, the existence of highly hydrophobic substrates will definitely restrict the implementation of any future sustainable conservation effort.

NANOWIRE OXIDE FILM FOR LOW-TEMPERATURE ALUMINIZED 20 STEEL BY THERMAL OXIDATION

An aluminized coating for low-carbon steel with good corrosion and wear resistance was first prepared through low-temperature pack aluminization. Then, the low-temperature-aluminized steel substrate was subjected to thermal oxidation in air. The phase composition, surface morphology, roughness, and elemental distribution of the aluminized carbon steel both before and after thermal oxidation were analyzed through X-ray diffraction spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The corrosion resistance and wear resistance of the original carbon steel substrate, aluminized carbon steel, and oxidized carbon steel were tested. Results showed that nanowires composed of iron oxide and alumina formed in situ on the top layer of the aluminized carbon steel. The corrosion resistance and wear resistance of the low-carbon steel with the nanowire oxide coating were better than those of the original carbon steel and aluminized carbon steel because the in-situ nanowire oxide film improved the density of the aluminized coating.

Flexible PLZT antiferroelectric film capacitor for energy storage in wide temperature range

Flexible energy-storage capacitor has attracted great attention due to deformable and lightweight, which could be applied to wearable electronics, bendable smartphones. It is urgent to manufacture a capacitor combining excellent electrical properties with mechanical bending capacity. Here, flexible Pb0.97La0.02Zr0.95Ti0.05O3 (PLZT 2/95/5) films are prepared by controlled spalling technology (CST) and exhibit outstanding electrical properties with polarization about 86.3 µC/cm2 at various tensile or compressive bending radii, which indicates excellent mechanical stability of films. It is worth mentioning that recoverable energy-storage density (Wre) of films has increased by 21% at 500 kV/cm compared to the original substrate, which is caused by change in stress. Meanwhile, flexible films were measured under wide temperature range from −70–200 °C with Wre fluctuating from 9.2 J/cm3 to 17.1 J/cm3, which is related to free energy barrier of phase transition. These studies provide reference for preparation of flexible capacitors which have great potential applications for future flexible micro-devices.

Phosphorus retention in constructed wetlands enhanced by zeolite‐ and clinopyroxene‐dominated lava sand

AbstractConstructed wetlands (CWs) are engineered systems for treating wastewater by sequestering nutrients and contaminants. Our aim was to assess the main phosphorus (P) binding states in operating CWs to assess P saturation and indications on P recycling potential of filter materials, which might be necessary under future peak P scenarios. The investigated vertical flow CWs (operation time up to 16 years) are based on either fluviatile (Fluv) sand or zeolite‐ (Ze‐LS) and clinopyroxene (Cl‐LS)‐dominated lava sand. Organic and inorganic P accumulated in all CWs independent of filter materials and showed a considerable increase with operation time. Concentrations of P decreased sharply with depth in the Fluv‐CWs compared to only a slight decrease in the lava sand CWs, with P concentrations of deeper horizons approximating the relatively P enriched original lava sand substrates. Orthophosphate was the dominant pool in all CWs, while the sum of organic fractions ranged between 11% and 33%. Sequential extraction indicated that P was mainly associated with Fe and Al (hydr)oxides for Fluv‐CWs and Ze‐LS‐CWs, while Ca and Mg bound mineral phosphates dominated in Cl‐LS‐CWs. Oxalate extractions pointed to a clear dominance of P fractions associated with poorly crystalline Fe‐ and Al‐(oxy)hydroxides. Solution 31P NMR analyses revealed that inositol hexakisphosphates were a major pool of organic P in surface layers of CWs, which increased with operation time. With a maximum of 0.5% P content, filter sands do not appear to be a suitable fertilizer for direct application to agricultural fields. The dominance of inorganic, poorly crystalline P species point to potentially high desorption capacity which might be investigated further, to assess recycling potential of P or usage of filter materials as soil amendments with relatively high plant available P. The latter might become feasible and economically attractive under future P scarcity. Simultaneously, P saturation indexes (DPS) did not indicate an imminent P saturation of filters, since P accumulation was not restricted by binding to Al and Fe minerals.

Towards timing and stratigraphy of the Bronze Age burial mound royal tomb (Königsgrab) of Seddin (Brandenburg, northeastern Germany)

Abstract. This study uses an integrated multi-method geoarcheological and geochronological approach to contribute to the understanding of the timing and stratigraphy of the monumental burial mound royal tomb (Königsgrab) of Seddin. We show that the hitherto established radiocarbon-based terminus post quem time frame for the construction of the burial mound of 910–800 BCE is supported by optically stimulated luminescence (OSL) dating. The radiocarbon samples were obtained from a substrate directly underneath the burial mound which supposedly represents the late glacial/Holocene soil that was buried below the structure. We use sedimentological (grain-size analyses) and geochemical analyses (element analyses, carbon, pH, and electric conductivity determinations) to reassess and confirm this hypothesis. In addition to the burial age associated with the last anthropogenic reworking during construction of the burial mound, the OSL dating results provide new insights into the primary deposition history of the original substrates used for the structure. In combination with regional information about the middle and late Quaternary development of the environment, our data allow us to provide a synoptic genetic model of the landscape development and the multiphase stratigraphy of the royal tomb of Seddin within the Late Bronze Age cultural group “Seddiner Gruppe” of northern Germany. Based on our initial experiences with OSL dating applied to the sediments of a burial mound – to the best of our knowledge the first attempt in Europe – we propose a minimal invasive approach to obtain datable material from burial mounds and discuss related opportunities and challenges.

A New Method for Weld Dilution Calculation through Chemical Composition Analysis

Aiming at existing methods unable to reveal the definition of dilution in welding accurately, such as the limited formula’s range of 0~1 mathematically, a new way which can be widely applied to various welds alloying either one or more compositions was proposed. Dilution values obtained using different methods through chemical composition analysis were compared and the effect of heat inputs on dilution was researched. The results show that the proposed method fabricated mathematically agrees well with the definition of dilution in welding. Especially, by dilution increasing to a level higher than the critical value, it reveals the fact that a weld can be concentrated as well when the filler wire is rich in the same composition as the substrate does. Besides, the method demonstrates that the gross dilution for a bead is a combined result that is affected by each individual composition. However, whether a composition is diluted or concentrated, composition’s concentrations are propelled by the increase of heat input toward that of the original substrate. During the process, the dilution value changes sharply before the heat input reaches the transition point (TP) of 0.18 kJ/mm, while once the heat input is beyond TP, the dilution becomes stable.

Charged ultrafiltration membranes based on TEMPO-oxidized cellulose nanofibrils/poly(vinyl alcohol) antifouling coating.

This study reports the potential of TEMPO-oxidized cellulose nanofibrils (T-CNF)/poly(vinyl alcohol) (PVA) coatings to develop functionalized membranes in the ultrafiltration regime with outstanding antifouling performance and dimensional/pH stability. PVA acts as an anchoring phase interacting with the polyethersulfone (PES) substrate and stabilizing for the hygroscopic T-CNF via crosslinking. The T-CNF/PVA coated PES membranes showed a nano-textured surface, a change in the surface charge, and improved mechanical properties compared to the original PES substrate. A low reduction (4%) in permeance was observed for the coated membranes, attributable to the nanometric coating thickness, surface charge, and hydrophilic nature of the coated layer. The coated membranes exhibited charge specific adsorption driven by electrostatic interaction combined with rejection due to size exclusion (MWCO 530 kDa that correspond to a size of ∼35–40 nm). Furthermore, a significant reduction in organic fouling and biofouling was found for T-CNF/PVA coated membranes when exposed to BSA and E. coli. The results demonstrate the potential of simple modifications using nanocellulose to manipulate the pore structure and surface chemistry of commercially available membranes without compromising on permeability and mechanical stability.

THE PROSPECTS OF USING ION-PLASMA MODIFICATION OF POLYURETHANES FOR CREATING DEFORMABLE BIOMEDICAL PRODUCTS

Plasma treatment is a promising method for modifying materials. However, deformation of the elastic substrate can damage the modified layer that can lead to various undesirable consequences. Elastic polyurethanes (elastic modulus up to 30 MPa) were studied; the polymers were treated by plasma immersion ion implantation of nitrogen ions with energy from 0,1 to 3 keV. Materials were investigated by atomic force microscopy, spectroscopy, the wettability and free energy of the surface were measured. Mechanical uniaxial tests were carried out and then surfaces were re-examined. Proteins and bacteria adsorption was studied, including the damaged surfaces under mechanical loads. As a result of the treatment with ion energy from 1 keV, the relief gets folded, the stiffness and hydrophobicity of the surface significantly increases, the adhesion of both gram-positive (staphyloco-ccus) and gram-negative (Escherichia coli) bacteria decreases. Cyclic uniaxial deformation causes transverse cracks and longitudinal folds on treated surfaces. Such changes provoke the bacterial growth to values greater than their number on untreated materials. The lower plasma energies (up to 0,5 keV) resulted in a slight increase in the stiffness of the modified layer (by 1,5 ... 2 times compared to untreated material), which made it possible to avoid surface damage under stretching. Due to the increased hydrophobicity and surface energy, the treated materials showed improved protein adsorption, the number of bacteria has decreased. All the surface changes (treatment, damage) correlate both with the properties of the original substrate (polyurethane is a block copolymer) and with the treatment mode.

BIOTECHNOLOGICAL ASPECTS OF BIOCONVERSION OF POST-EXTRACTION BIOMASS AND CELL CULTURE ORTHILIA SECUNDA (L.) HOUSE WITH BASIDIOMYCETES

The possibility of bioconversion after the extraction residue of the medicinal plant Orthilia secunda (initial plant biomass and callus after extraction of biologically active substances) by fast growing strains of basidiomycetes Tv2-16K Trametes versicolor and Pe-17T Pleurotus eryngii was studied. The main target products are: a mycelial-plant product with a reduced content of the indigestible fraction and enriched in protein and full-fledged fruit bodies. Fungi colonize plant waste with a radial growth rate of 2.0–2.3 mm / day and a growth rate of 65–77 units. Phenol oxidase activity is 0.7–1.2 units/g·s, depending on the type after the extraction residue. In substrates after biodegradation, the proportion of difficult hydrolysable polysaccharides, easily hydrolysable polysaccharides, and lignin is on average 1.6 times lower than in the original substrate. The content of protein and extractive substances is significantly higher, especially after the extraction residue of O. secunda callus under the influence of the T. versicolor strain Tv2-16K: 12.8 and 24.3%, respectively, of the mass of dry substrate. Fruit bodies of P. eryngii strain Pe-17T were obtained on various compositions of plant substrates. The maximum fruit formation was noted on four-component substrates containing two types after extraction residues of O. secunda, wheat bran, birch or aspen sawdust: the average weight of fruit bodies from one block was 230–236 g; biological efficiency – 46–47.2%.

Structural and Functional Analyses of a Spiro-Carbon-Forming, Highly Promiscuous Epoxidase from Fungal Natural Product Biosynthesis.

Biosynthesis of fungal nonribosomal peptides frequently involves redox enzymes such as flavin-containing monooxygenase (FMO) to introduce complexity into the core chemical structure. One such example is the formation of spiro-carbons catalyzed by various oxidases. Because many chemically complex spiro-carbon-bearing natural products exhibit useful biological activities, understanding the mechanism of spiro-carbon biosynthesis is of great interest. We previously identified FqzB, an FMO from the fumiquinazoline biosynthetic pathway responsible for epoxidation of fumiquinazoline F that crosstalks with the fumitremorgin biosynthetic pathway to form spirotryprostatin A via epoxidation of the precursor fumitremorgin C. What makes FqzB more interesting is its relaxed substrate specificity, where it can accept a range of other substrates, including tryprostatins A and B along with its original substrate fumiquinazoline F. Here, we characterized FqzB crystallographically and examined FqzB and its site-specific mutants kinetically to understand how this promiscuous epoxidase works. Furthermore, the mutagenesis studies as well as computational docking experiments between the FqzB crystal structure and its known substrates spirotryprostatin A and B, as well as fumitremorgin C and fumiquinazoline F, provided insight into potential modes of substrate recognition and the source of broad substrate tolerance exhibited by this epoxidase. This study serves as a foundation for further characterization and engineering of this redox enzyme, which has potential utility as a valuable catalyst with broad substrate tolerance and an ability to introduce chemical complexity into carbon frameworks for chemoenzymatic and biosynthetic applications.

Direct photoreactive immobilization of water-soluble phospholipid polymers on substrates in an aqueous environment

The purpose of this study is to achieve a simpler and safer surface modification of substrates using a photoreactive polymer in an aqueous environment. We synthesized water-soluble photoreactive polymers with both phenylazide groups and phosphorylcholine groups, poly(2-methacryloyloxyethyl phosphorylcholine-co-4-methacryl tetra(ethylene glycol)oxycarbonyl-4-phenylazide) (PMEPAz), via reversible addition fragmentation chain transfer polymerization. PMEPAz with different polymerization degrees were synthesized with a well-defined structure. To immobilize PMEPAz on the substrate surface by photoreaction, it is necessary to adsorb the polymer on the substrate surface in an aqueous solution because the phenylazide groups chemically bind to the substrate via a hydrogen abstract reaction. The relationship between the polymer solubilization state in the aqueous solution and the adsorption behavior at the surface was investigated. PMEPAz began to form unstable molecular aggregates at a concentration of 10−2 mg/mL and formed stable aggregates at 100 mg/mL. At a concentration of 10−1 mg/mL, unstable molecular aggregates of PMEPAz were formed in the aqueous solution, resulting in the maximization of the amount of adsorbed polymer and effective photoreaction with the substrate. The thickness of the reacted polymer layer on the substrate increased with an increase in the polymerization degree, a uniform polymer layer with a thickness of 3.4 nm was formed when the polymerization degree was 400. After surface modification, the hydrophobic surfaces of the original substrates became hydrophilic. Additionally, fibrinogen adsorption and platelet adhesion were effectively suppressed based on the characteristics of the phosphorylcholine unit.