Origin State Research Articles

Diagnostic, prognostic, and therapeutic potential of exosomal microRNAs in renal cancer.

Renal cell carcinoma (RCC) arises from the tubular epithelial cells of the nephron. It has the highest mortality rate among urological cancers. There are no effective therapeutic approaches and no non-invasive biomarkers for diagnosis and follow-up. Thus, suitable novel biomarkers and therapeutic targets are essential for improving RCC diagnosis/prognosis and treatment. Circulating exosomes such as exosomal microRNAs (Exo-miRs) provide non-invasive prognostic/diagnostic biomarkers and valuable therapeutic targets, as they can be easily isolated and quantified and show high sensitivity and specificity. Exosomes secreted by an RCC can exhibit alterations in the miRs' profile that may reflect the cellular origin and (patho)physiological state, as a ''signature'' or ''fingerprint'' of the donor cell. It has been shown that the transportation of renal-specific miRs in exosomes can be rapidly detected and measured, holding great potential as biomarkers in RCC. The present review highlights the studies reporting tumor microenvironment-derived Exo-miRs with therapeutic potential as well as circulating Exo-miRs as potential diagnostic/prognostic biomarkers in patients with RCC.

Spontaneous hydropneumothorax with unknown origin and new onset hyperosmolar hyperglycemic state: case report in limited health facility

Introduction: Hydropneumothorax and Hyperosmolar Hyperglycaemic State (HHS) are uncommon disorders with serious mortality. Both conditions need appropriate intervention and monitoring in comprehensive health facilities. Reports on the occurrence of spontaneous hydropneumothorax accompanied by HHS are still limited, due to the rarity of these two disorders.Case Presentation: A 42-year-old woman with abrupt shortness of breath started 1 day ago. No coughing or chest pain. Losing weight in last six months. There was no history of any disease and medication. Physical findings were drowsy consciousness with tachycardia and weak pulse, asymmetrical chest expansion with retraction, vesicular breath sounds diminished in the left lung following hyper resonant in percussion. The extremities were cold and dry. Skin turgor was reduced. Plain CXR revealed air and fluid in left pleural cavity. Laboratory test revealed very high blood glucose with hyperosmolarity.Discussion: Hydropneumothorax is the presence of air in the pleural space accompanied by ipsilateral effusion. The manifestations include shortness of breath, increased work of breathing, and sharp chest pain, or asymptomatic. It is unclear whether this patient's hyperglycaemia is related to the occurrence of spontaneous hydropneumothorax. HHS can be diagnosed by effective serum osmolality with glucose levels plus appropriate medical presentation. The management is based on the correction of dehydration, hyperglycaemia, hyperosmolarity, and precipitating conditions.Conclusion: Both hydropneumothorax and HHS have high mortality rates, particularly when the two conditions coexist. This case met some challenging issues that might increase the mortality.

311 Evaluation of Post-Transit Growth Performance and Carcass Characteristics of Feedlot Heifers Sourced and Finished in Different Regions of the U.S

Abstract The objective was to evaluate growth performance and carcass traits following transit of feedlot heifers sourced and finished in different geographical regions in the U.S. Yearling heifers [n = 190; initial body weight (BW) 483 and 425 kg for SD and TX sourced, respectively] were used in a 2×2 factorial arrangement of origin state (SD vs TX) and finishing state (SD vs TX). Heifers were allotted on d -1 into four treatments: sourced from SD and finished in SD (SD-SD), sourced from SD and finished in TX (SD-TX), sourced from TX and finished in SD (TX-SD), and sourced from TX and finished in TX (TX-TX). Heifers were weighed on d -1, 3, 15, 28, 56, 78 (TX-TX and SD-TX) and 90 (SD-SD and TX-SD). On d 0, SD-TX and TX-SD heifers were shipped to the finishing location and weighed the following morning (d 1) to determine transportation shrink. To monitor transportation effects, vaginal temperature probes were inserted into all SD-TX and TX-SD heifers and a portion of SD-SD and TX-TX heifers on d -1 and removed on d 3. Clinical attitude scores (CAS) were recorded on d -1, 0, 1, 2 and 3 for indications of bovine respiratory disease symptoms. The GLIMMIX procedure of SAS was used to analyze growth performance and carcass measures with fixed effects of origin and finishing state. The MIXED procedure was used for CAS and temperature data with the same fixed effects and time as a repeated measure. Transported heifers had reduced temperatures (P < 0.05) during transit and post-transit compared with non-transported heifers. Temperatures of transported heifers increased (P < 0.05) during loading and unloading. On d 0, 1 and 3 there was a shift in the distribution of heifers that had a CAS score greater than 0 for TX-TX, SD-TX and TX-SD. Cattle endured high ambient temperatures (temperature humidity index value > 75) 54% and 18% of the feeding period for TX and SD finished heifers, respectively. All cumulative growth performance measures and carcass trait interactions were statistically significant (P < 0.05) besides initial BW, percent shrink of transported heifers, average daily gain, dressing percent, ribeye area and liver abscess severity, which were similar (P > 0.30). There was a shift in the distribution (P < 0.05) towards a greater proportion of Yield Grade 1 and Select carcasses for heifers fed in TX compared with those fed in SD, likely due to fewer days on feed. Overall, heifers transported to higher ambient temperatures had reduced dry matter intake, quality grades (QG) and limited growth recovery (45 kg lighter) compared with non-transported heifers. Heifers transported to lower ambient temperatures recovered growth and had improved QG at the same level of rib fat compared with non-transported heifers.

Comparison of 624-type Capillary Columns

In our research, seven 624-type capillary columns were investigated. All the columns were the same in length, internal diameter, and film thickness (30 m × 0.32 mm × 1.0 µm). However, they were produced by different manufacturers or the same manufacturer but in different batches. Even though the manufacturers recommend them as "equivalent columns" this equivalence did not prevail even in the case of columns produced by the same manufacturer. Our examination criteria centered on the quantitative determination ability of the columns. A homemade column test mixture was compiled to represent all the second-order interactions that can occur between the analyte and stationary phase. Although theoretically these columns have the same stationary phase quality, they did not result in the same chromatograms. In addition to the origin and batch of the column, the "history of the column" contributes likewise to the different peak symmetry, retention order, and even peak areas that affect the quantitative determination. We quantified this quantitative determination ability with the effective carbon number (ECN) and the Limit of Quantitation (LoQ) values. Based on our results the attainable LoQ and ECN values depend at least as much on the origin and actual state of the stationary phase as on the measurement conditions to be optimized. In our paper, we demonstrate the extent to which the same stationary phases offered by different companies and/or different backgrounds can influence our detection limit and detector response even if the relevant columns have theoretically the same chemical structure.

Migrant networks, regional development and internal migration flows in Brazil

PurposeThis study analyzes the role of migrant networks in the migration flows in relation to the educational level of the migrants and economic growth of the states of origin and destination in Brazil.Design/methodology/approachFixed effects estimator applied to microdata.FindingsThe results show migrant networks have a significant and positive impact on migration flows of the different educational levels. The economic growth in the destination state accentuates this effect, while the economic growth in the origin state has distinct impacts according to the educational level of the new migrant.Originality/valueThe authors investigate the importance of migrant networks in the internal immigration within a developing country with large internal movement of people. In Brazil, the socio-economic condition of the population varies considerably in relation to its geography, which explains the country’s large internal migration flows.

Origin, Emergence, Formation, Development, and Current State of Organized Crime in Ukraine

The Criminal Code (hereinafter CC) of the Ukrainian SSR (§2, Art.41) only once provided for a crime committed by an organized group as an aggravating circumstance in any crime till 1989 [1]. At the close of 80-s and in 90-s especially the country faced a fast-growing and serious challenge from organized crime that caused a classifying circumstance - a commission by an organized criminal group (hereinafter OCG) amended in 20 articles, the Special Part, CC. Regulations, Session of the Supreme Court and Methodical Instructions, the Ministry of Interior did not give an appropriate definition of OCG. As a result law enforcement and, first of all, the Ministry of Interior agencies did not have strict legislative acts to define OCG from traditional groups of criminals. All the cited above could not but adversely affect the struggle against organized crime. According to the statistical data for 1991-2023, annual Reports on combating organized criminal groups, the Ministry of Interior the status of organized crime is as follows.

The role of heritage sites and other influential factors in domestic tourism inflow to Australian states: a gravity model approach

ABSTRACT Domestic tourism plays a crucial role in the Australian economy, generating revenue, creating employment opportunities, fostering cultural identity, and facilitating tourism growth and development. The remote regions of Australia are particularly reliant on domestic inbound tourism to stimulate their local economies. This study investigates the influence of heritage sites and various factors on domestic tourism inflows to eight states in the Australia between 1998–2021. The gravity method and random effect model are employed for the empirical analysis. The results indicate that the macro determinants, including population of origin state, gross state product per capita, infrastructural development, shared border between states, and the number of heritage sites, have significant and positive impact on domestic tourism inflow. Conversely, the consumer price index, distance, and pandemic outbreak have a negative influence on domestic tourism inflow. These findings hold important practical implications. Given Australia’s geographical remoteness, promoting domestic tourism becomes imperative to boost the tourism industry and local economies. Therefore, it is recommended that authorities prioritize domestic tourism flows and invest in infrastructure, preserve heritage sites, stabilize prices, implement effective marketing strategies, and respond swiftly to public emergencies such as the Covid-19 pandemic.

Origin, Evolution and Current State Russian-Chinese Foreign Trade Relations

The article examines Russian-Chinese foreign trade relations, their origin, development and current state. The official approaches of the states to the organization, subsequent development and strengthening of foreign trade relations, established by Nerchinsky (1689), Kyakhtinsky (1727), Tianjin (1858), Beijing (1860), Moscow (1896) are given contracts and other documents. The current state, indicators of the Russian-Chinese foreign trade turnover in 2020–2022 are disclosed. and priority areas of mutual foreign economic cooperation between states. The main projects of investment cooperation are given. The conclusions summarize the impact of Russian-Chinese foreign trade cooperation on improving the efficiency of the implementation of programs for their socio-economic development.

Historical origin and current state of research gender equality in law enforcement bodies of Ukraine

Security sector reform is aimed at transforming the security sector to increase accountability, efficiency, humanism, the rule of law and gender equality in the structural units of the Ministry of Internal Affairs of Ukraine. One of the structural units of the Ministry of Internal Affairs of Ukraine is the National Police. Gender parity in the National Police is the key to ensuring the implementation of the reform and compliance with global trends in the development of the State. The purpose of the article is to carry out a theoretical analysis of scientific research on gender equality in law enforcement agencies of independent Ukraine from 1991 to the present. The methodological basis of the study is the fundamental principles of ensuring equal rights and opportunities for men and women in society. The study used the following methods of scientific research (cognition): theoretical research methods (ascent from the abstract to the concrete, transition from the concrete to the abstract), empirical research methods (comparison), complex research methods (abstraction, analysis and synthesis, induction and deduction), which contributed to the achievement of the research objective. The stages of scientific research on the problem of ensuring gender equality in law enforcement agencies of Ukraine are identified and substantiated: the first stage (1991 - October 2004); the second stage (October 2004 - November 2015); the third stage (November 2015 - present). It is determined that scientific research on the issue of gender equality in law enforcement agencies of independent Ukraine concerned the legal regulation of the work of women law enforcement officers and their social and legal protection, gendered styles of behavior of law enforcement officers, their psychophysiological differences and gender equality, psychological conditions for ensuring gender equality in law enforcement activities, and peculiarities of development of gender relations in interpersonal communication of law enforcement officers. Three groups of problematic issues arising in the work of a female law enforcement officer serving in the National Police of Ukraine are identified and described in detail. The practical significance of the study lies in the thorough analysis of scientific research on the issue of gender equality in law enforcement agencies of independent Ukraine and the identification of unresolved issues

Colorimetric Assaying of Exosomal Metabolic Biomarkers.

Exosomes released into the extracellular matrix have been reported to contain metabolic biomarkers of various diseases. These intraluminal vesicles are typically found in blood, urine, saliva, breast milk, cerebrospinal fluid, semen, amniotic fluid, and ascites. Analysis of exosomal content with specific profiles of DNA, microRNA, proteins, and lipids can mirror their cellular origin and physiological state. Therefore, exosomal cargos may reflect the physiological processes at cellular level and can potentially be used as biomarkers. Herein, we report an optical detection method for assaying exosomal biomarkers that supersedes the state-of-the-art time consuming and laborious assays such as ELISA and NTA. The proposed assay monitors the changes in optical properties of poly(3-(4-methyl-3'-thienyloxy) propyltriethylammonium bromide) upon interacting with aptamers/peptide nucleic acids in the presence or absence of target biomarkers. As a proof of concept, this study demonstrates facile assaying of microRNA, DNA, and advanced glycation end products in exosomes isolated from human plasma with detection levels of ~1.2, 0.04, and 0.35 fM/exosome, respectively. Thus, the obtained results illustrate that the proposed methodology is applicable for rapid and facile detection of generic exosomal biomarkers for facilitating diseases diagnosis.

The Colección Osteológica Subactual de Santiago: Origin and Current State of a Documented Skeletal Collection from Chile, Latin America

This manuscript aims to introduce the Colección Osteológica Subactual de Santiago (COSS), a documented skeletal collection from Santiago, Chile, consisting of 1635 individuals living in low socioeconomic areas of the capital during the late 19th and early 20th centuries. The origin and current state of this collection, as well as the process by which it came to be housed at the University of Chile, is described. As of today, after long efforts for further documentation and improvements on the physical conditions of the collection, biological sex and age-at-death has been documented through burial records for 1198 individuals. Largely studied by Chilean researchers and students, the COSS collection has enabled a great amount of research, while also serving as part of a thriving scholarly community from different disciplines. Finally, discussion around representativeness, legal status and ethical concerns are addressed, highlighting the specific issues faced when working and studying the COSS collection.

A historical narrative review of paired oral reading practices in elementary classrooms

BackgroundPaired Reading (PR), Neurological Impress Method (NIM), Dyad Reading (DR), and Read Two Impress (R2I) share a fundamental trait – paired synchronous oral reading between a higher‐level tutor and a lower‐level tutee. Collectively, we refer to these practices as Synchronous Paired Oral Reading Techniques (SPORT). This historical narrative review investigates the origin, development, and current state of SPORT.MethodsFive academic databases were searched for reports of SPORT from the years 1960–2021. This process yielded 3972 results, from which 456 full‐text articles were assessed for eligibility. Finally, 77 publications were reviewed to inform this historical narrative.ResultsFour distinct waves of SPORT research dating back to 1966 were identified in the review. These waves are characterised by popularity in the United Kingdom and United States, evolving theoretical frameworks, varying configurations and implementations, and concerted focus on reading achievement. Consistently positive outcomes are reported in the SPORT literature, specifically in oral reading fluency and reading comprehension.ConclusionsThe theory and practice of SPORT have evolved significantly throughout its extensive history. We call for a fifth wave of SPORT research to consolidate the findings of the last 60 years, develop new lines of inquiry, and implement novel and varied applications of SPORT.

Interstate Migration in India During the COVID-19 Pandemic: An Analysis Based on Mobile Visitor Location Register and Roaming Data

In migration and mobility studies, the availability of scientifically reliable data remains a persistent challenge. The recent move towards harnessing mobile and big data has also been unable to resolve the data issues due to accessibility, privacy, as well as ethical and methodological intricacies involved with such data sets. In this paper, we explore a new set of data known as visitor location register (VLR) and roaming data, which is recorded and reported by mobile service providers. The reporting model of VLR data used and presented in this paper is not only free from privacy and ethical concerns but also methodologically sound and simple to compute as compared to any previous approaches. Drawing on VLR data, this paper finds direct evidence of unusually high interstate net reverse migration during the first and second COVID-19 lockdowns in India (44.13 and 26.3 million, respectively), and thereafter quick return migration back to cities during unlocks. The findings from this paper also provide insights into evolving migration directions, precarity, pockets of origin and destination and state policies in containing reverse migration during lockdowns in India. We anticipate that the data presented in the paper have the potential to fill a major data gap in migration and mobility studies in other countries too if VLR and roaming data are made available at the required spatial and temporal levels.

Nanoparticle and Nanostructure Synthesis and Controlled Growth Methods.

Nanomaterials are materials with one or more nanoscale dimensions (internal or external) (i.e., 1 to 100 nm). The nanomaterial shape, size, porosity, surface chemistry, and composition are controlled at the nanoscale, and this offers interesting properties compared with bulk materials. This review describes how nanomaterials are classified, their fabrication, functionalization techniques, and growth-controlled mechanisms. First, the history of nanomaterials is summarized and then the different classification methods, based on their dimensionality (0–3D), composition (carbon, inorganic, organic, and hybrids), origin (natural, incidental, engineered, bioinspired), crystal phase (single phase, multiphase), and dispersion state (dispersed or aggregated), are presented. Then, the synthesis methods are discussed and classified in function of the starting material (bottom-up and top-down), reaction phase (gas, plasma, liquid, and solid), and nature of the dispersing forces (mechanical, physical, chemical, physicochemical, and biological). Finally, the challenges in synthesizing nanomaterials for research and commercial use are highlighted.

Malleability of Alcohol Consumption: Evidence from Migrants

How malleable is alcohol consumption? Specifically, how much is alcohol consumption driven by the current environment versus individual characteristics? To answer this question, we analyze changes in alcohol purchases when consumers move from one state to another in the United States. We find that if a household moves to a state with a higher (lower) average alcohol purchases than the origin state, the household is likely to increase (decrease) its alcohol purchases right after the move. The current environment explains about two-thirds of the differences in alcohol purchases. The adjustment takes place both on the extensive and intensive margins.

Diasporas and Collective Remittances: From State-Driven to Unofficial Forms of Diaspora Engagement

This article advances understandings of collective remittances practices by elaborating on the case of the Armenian diaspora in the Netherlands. It argues that the collection, management, and distribution of collective remittances are crucial aspects of how diasporic immigrant groups function. Hence, collective remittances represent a lens through which to analyze a diaspora's internal life, gender differences and tensions, relations with the origin state or homeland, and connections to other relevant institutions. Furthermore, the article brings three new insights to diaspora studies. First, it demonstrates how practices connected to the collection and sending of collective remittances reflect the internal dynamics of diasporic migrations and diaspora-homeland relations. Second, the article unpacks the concept of collective remittances itself, classifying it into three categories: collective remittances initiated from above, with a state-driven character; collective remittances organized by transnational non-state institutions, such as religious institutions and pan-diasporic organizations; and collective remittances initiated from below, organized by migrants and local networks of diaspora members. Third, this article highlights women's roles in organizing alternative forms of collective remittances, from external to official channels. The qualitative examination of collective remittances presented here supplies a new vision of collective remittances in which informal and hidden aspects, gender roles, forms of reciprocation, and creation and circulation of trust are highlighted.

Identification of N-methyl-D-aspartate receptor antagonists using the rat postnatal mixed cortical and hippocampal neurons

The goal of this study was to evaluate mixed cortical and hippocampal primary rat postnatal neuronal culture as in vitro tool for identification of N-methyl-D-aspartate receptor (NMDAR) antagonists and to find out, whether this model is comparable with other commonly used primary rat neuronal models differing in their origin (pure cortical vs. mixed cortical and hippocampal) and differentiation state (embryonal vs. postnatal). Induced pluripotent stem cell (iPSC) – derived human glutamatergic neurons have been included in this study as well. First, the cultures were characterized by their neuron/astrocyte composition, the mRNA expression of NR2B/NR2A NMDAR subunit ratios, and the expression of glutamate transporters (GLT1, GLAST). Then, selected endogenous steroids and synthetic neuroactive steroids that have been previously identified as negative allosteric modulators of recombinant GluN1/GluN2B NMDA receptors, were evaluated for their ability to prevent an NMDA or glutamate-induced Ca2+ influx (acute effect) and excitotoxicity over 24 h. Though the neuroprotective potential against excitotoxic stimuli varied among the models studied, postnatal mixed cortical and hippocampal culture proved to be a convenient and robust tool for NMDAR antagonist screening. The most widely used embryonal (E18) cultures offered higher cell yields but at the expense of a higher sensitivity to compounds’ cytotoxicity. iPSC-derived neurons were not found to be superior to rat cultures for screening purposes.

Mass media of the indigenous peoples of the Far East: the history of origin and current state

The purpose of the article is to study the history of the emergence of the mass media of the indigenous peoples of the Far East and their current state. The author examines the factors of formation, functions and purpose, formal and substantive features, the main trends in the development of mass media of the indigenous peoples of the Far East in the context of the political, economic and socio-cultural situation of the XIX - XXI centuries. The geography of the study covers the entire Far Eastern Federal District and includes an analysis of the content of ethnic media operating on the territory of the Republic of Sakha (Yakutia), the Republic of Buryatia, the Khabarovsk Territory, the Sakhalin Region and other subjects of the Far Eastern region. The research, being philological in essence, is based on an interdisciplinary approach, synthesizing knowledge of various sciences: theory and history of journalism, ethnography, ethnology, cultural studies. General scientific methods (descriptive-analytical, system description, classification construction method), as well as special methods (historical, comparative, content analysis method), elements of the synchronic and diachronic method were used as the main research methods. The history of the formation of ethnic media and the problem-thematic originality of the national media of the Far Eastern region have not yet become the object of a comprehensive study of domestic scientists. The novelty of the work consists in a comprehensive study of the mass media of the indigenous peoples of the Far East in the historical and typological aspect, clarifying the qualitative characteristics of the Far Eastern media in the context of the formation of an actual structure of regional journalism.

Single-particle characterization of polycyclic aromatic hydrocarbons in background air in northern Europe

Abstract. We investigated the distribution of polycyclic aromatic hydrocarbons (PAHs) on individual ambient aerosol particles at the Swedish western coast in a pristine environment for 10 d in October 2019. The measurements were carried out using new technology with single-particle mass spectrometry (SPMS) that reveals both the inorganic particle composition as well as the particle-bound PAHs (Schade et al., 2019). More than 290 000 particles were characterized; 4412 of them reveal PAH signatures. Most of the PAH-containing particles were internal mixtures of carbonaceous material, secondary nitrate and metals from distant sources in central and eastern Europe. We characterize the aerosol with respect to the inorganic composition, comparable to conventional SPMS, before we discuss the distribution of PAHs within this particle ensemble. Vice versa, we analyze the single-particle PAH spectra for characteristic patterns and discuss the inorganic composition, origin and atmospheric processing of the respective particles. The study period comprised different meteorological situations: clean air conditions with winds from the North Sea/Kattegat and little terrestrial air pollution, long-range transport from eastern Europe and southern Sweden, and transport of aerosols from central Europe over the sea. For all meteorological conditions, PAHs were detected in particles whose inorganic content indicates traffic emissions, such as combinations of soot, iron and calcium as well as in particles with biomass-burning signatures. However, there were variations in their amounts, dependent on the geographic origin. Because of strong mixing, rapid degradation and speciation limits, e.g., for PAHs of the same nominal mass, the application of diagnostic ratios for source apportionment is limited under the conditions of our study. Nevertheless, the combination with the inorganic content and meteorological data provides unique insights into the particles' origin, aging and mixing state. We exemplarily show how the observation of PAH profiles and inorganic secondary components on a single-particle level can open a new door to investigate aerosol aging processes. To our best knowledge, we herewith present the first comprehensive study on the single-particle distribution of PAHs in ambient air as well as the first set of combined data on PAHs and inorganic composition on a single-particle level.

Selective migration in American interstate residential flow: Similarity of Big Five personality factors among origin and destination state residents

Two studies using the 48 contiguous American states tested the general hypothesis that the percent of the total number of movers leaving an origin state that migrates to a particular destination state is related to the degree of similarity in Big Five personality between the residents of the origin state and the destination state. Datasets for 2005–2006 and 2016–2017 were analyzed. The hypothesis was tested using Pearson correlation and multiple regression strategies without and with consideration of the following state-level statistical controls: socioeconomic status based on two economic and two educational variables, unemployment rate, White population percent, urban population percent, conservatism, and road distance between state capitals for the 48 states. A consistent pattern of support for the hypothesis was found for each of the Big Five personality dimensions—openness to experience, conscientiousness, extraversion, agreeableness, and neuroticism—for both datasets without and with statistical controls. Results without statistical controls demonstrated that movers from states with residents higher on a Big Five personality dimension indeed are more likely to migrate to states with residents higher on that personality dimension, and that movers from states with residents lower on a personality dimension are more likely to migrate to states with residents lower on that personality dimension. Similar results were obtained with statistical controls but the relations for conscientiousness were in the supportive direction but not statistically significant. It is speculated that these state-level relations are grounded in parallel individual-level relations suggested by the theories of selective migration, homophily, similarity-attraction, and person-environment fit.