This study examined the flexural behavior of the poplar laminated veneer lumber (LVL) box floor with orthogonal rib beams. Four 3.6 m × 4.8 m box floor samples made of poplar LVL orthogonal rib beams and oriented strand board (OSB) plates were tested under vertical uniform loading, from which the bearing capacity, stiffness, and failure characteristics were analyzed. There was no damage in all box floor samples at the normal service load of 2.5 kN/m2, and the maximum deflection was far less than the allowable value. When the maximum load was applied, the load-displacement curve of each floor sample exhibited a linear relationship without obvious failure. However, localized failure was manifested as the dislocation slip of the rib beams relative to the upper and lower floor slabs at the corner nodes and the joint expansion and staggered floors at the bottom plate, with obvious failure signs. The rib beam height had the most significant impact on the floor stiffness, followed by the spacing of short-side rib beams, whereas the OSB plate thickness had lest impact. The mid-span deflections of poplar LVL orthogonal ribbed box floor samples, which were calculated using the analog slab method, were in good agreement with the experimental results with an error being less than 10%.
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