Orientalis Mill Research Articles

Five anti-inflammatory compounds from the resins of Liquidambar orientalis

Five undescribed compounds, including a triterpenoid (1), three phenylpropanoids [(±)−2 and 4], and an aromatic compound (3), as well as six known analogues (5–10), were isolated from the resins of Liquidambar orientalis Mill. Their structures, including absolute configurations, were determined by using spectroscopic and computational methods, and the five new compounds displayed anti-inflammatory activities in LPS-induced RAW264.7 cells.

Effects of grafting on chemical constituents, toxicological properties, antithrombotic activity, and myocardial infarction protection of styrax secreted from the trunk of Liquidambar orientalis Mill.

Styrax, the balsam refined from the trunk of Liquidambar orientalis Mill. has a variety of applications in the perfumery and medical industry, especially for use in traditional Chinese medicine. However, the resources of styrax are in shortage due to being endangered of this plant. Grafting can improve the adaptability of plants to unfavorable environmental conditions. We tried to graft the L. orientalis Mill. on L. formosana Hance which was widely distributed in Jiangsu and Zhejiang provinces of China in an attempt to obtain styrax from grafted L. orientalis Mill. (grafted styrax, SG). Whether SG can become an alternative application of commercially available styrax (SC) need be further investigated. The components of SG were analyzed by GC-MS, and the results showed that the chromatograms of SG, SC, and styrax standard (SS) were consistent. The ration of 12 major chemical components based peak area in SG, SC, and SS were 93.95%, 94.24%, and 95.86% respectively. The assessment of toxicity, antithrombotic activity, and myocardial infarction protection of SG and SC was evaluated by using the zebrafish model, the results showed that SG and SC have the similar toxicological properties as evidenced by acute toxicity test, developmental toxicity and teratogenicity, and long-term toxicity test. Both SG and SC significantly decreased the thrombosis and increased blood flow velocity of zebrafish induced by adrenaline hydrochloride, inhibited myocardial apoptosis, myocardial infarction and myocardial inflammation in zebrafish induced by isoproterenol hydrochloride. Moreover, SG had an obvious improvement effect on cardiac output, while SC has no effect. Collectively, SG is similar to SC in chemical composition, toxicological properties, antithrombotic activity, and myocardial infarction protection effects, and may be used as a substitute for styrax to reduce the collection for wild L. orientalis Mill. and increase the available styrax resources.

Bilayer wound dressing composed of asymmetric polycaprolactone membrane and chitosan-carrageenan hydrogel incorporating storax balsam

A comprehensive approach is needed to develop multifunctional wound dressing that is simple yet efficient. In this work, Liquidambar orientalis Mill. storax loaded hydroxyethyl chitosan (HECS)-carrageenan (kC) based hydrogel (HECS-kC) and polydopamine coated asymmetric polycaprolactone membrane (PCL-DOP) were used to develop a multifunctional and modular bilayer wound dressing. Asymmetric PCL-DOP membrane was prepared by non-solvent induced phase separation (NIPS) followed by polydopamine coating and demonstrated an excellent barrier against bacteria while allowing permeability for 5.45 ppm dissolved‑oxygen and 2130 g/m2 water vapor transmission in 24 h in addition to 805 kPa tensile strength. Storax loaded HECS-kC hydrogel, on the other hand, demonstrated a pH-responsive degradation and swelling to provide necessary conditions to facilitate wound healing. The hydrogels showed stretchability above 140 %, mild adhesive strength on sheep skin and PCL-DOP membrane, while the storax incorporation enhanced antibacterial and antioxidant activity. Furthermore, rat full-thickness skin defect model showed that the developed bilayer wound dressing could significantly facilitate wound healing compared to Tegaderm™ and control groups. This study shows that the bilayered wound dressing has the potential to be used as a simple and effective wound care system.

Evaluation of the Potential Therapeutic Properties of Liquidambar orientalis Oil.

Liquidambar orientalis Mill., commonly called the Anatolian sweetgum or Sigla tree, is endemic to southwestern Turkey. It has been historically significant in traditional medicine. In our research, we delved into the therapeutic attributes of its oil, emphasizing its antioxidant, antimicrobial, and antitumor properties. The primary chemical constituent of the gum is styrene, accounting for 78.5 %. The gum demonstrated antioxidant capabilities in several assays, including in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP). It displayed bactericidal actions against various gram-positive bacteria, such as Staphylococcus aureus, and gram-negative strains, including Escherichia coli. Additionally, the oil showcased potent antitumor effects against breast (MDA-MB-231), lung (A549), and prostate (PC3) cancer cell lines. These effects were found to be both time- and dose-dependent. L. orientalis Mill. oil showed the best antitumor activity against breast, lung, and prostate cancer cell lines after the 24 h and 48 h treatment. Its oil might induce autophagy in the PC3 prostate cancer cell line, whereas its cytotoxicity against MDA-MB-231 and A549 cancer cell lines might not be correlated with autophagy or apoptosis pathways. In conclusion, the oil from the Sigla tree offers promising therapeutic potential and warrants further exploration.

Increasing temperatures can pose an opportunity to recover endemic and endangered oriental sweetgum tree (Liquidambar orientalis Mill.) from extinction

Increasing temperatures can pose an opportunity to recover endemic and endangered oriental sweetgum tree (Liquidambar orientalis Mill.) from extinction

Floristic and ecological diversity of stands dominated by Carpinus orientalis in gorges and canyons of eastern Serbia (SE Europe)

We investigated stands dominated by Carpinus orientalis Mill. in five gorges and canyons across eastern Serbia. Floristic and ecological analyses were conducted on a dataset of 102 phytosociological relevés collected in the field and 33 relevés from the existing literature. Hierarchical classification distinguished four groups of phytosociological relevés within the dataset. Floristic composition, diversity and phytogeographical characteristics of the groups were determined. These groups occur in ecologically different habitats and differ with respect to ecological gradients. Non-metric multidimensional scaling revealed that the main gradients that influence the variation in the floristic composition were moisture, nutrients, temperature and light. The results obtained in our analyses allowed us to describe the new association Seslerio filifoliae–Carpinetum orientalis ass. nova hoc loco, that occupies very steep and stony slopes, and is developing at altitudes between 80 and 550 m a.s.l. in all aspects.

3D printed Styrax Liquidus (Liquidambar orientalis Miller)-loaded poly (L-lactic acid)/chitosan based wound dressing material: Fabrication, characterization, and biocompatibility results

The medicinal plant of Styrax liquidus (ST) (sweet gum balsam) which extracted from Liquidambar orientalis Mill tree, was loaded into the 3D printed polylactic acid (PLA)/chitosan (CS) based 3D printed scaffolds to investigate its wound healing and closure effect, in this study. The morphological and chemical properties of the ST loaded 3D printed scaffolds with different concentrations (1 %, 2 %, and 3 % wt) were investigated by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FT-IR), respectively. In addition, the mechanical and thermal properties of the materials were investigated by Tensile test and Differential Scanning Calorimetry (DSC), respectively. The antimicrobial activities of the ST loaded 3D printed scaffolds and their incubation media in the PBS (pH 7.4, at 37 °C for 24 h) were investigated on two Gram-positive and two Gram-negative standard pathogenic bacteria with the agar disc diffusion method. The colorimetric MTT assay was used to determine the cell viability of human fibroblast cells (CCD-1072Sk) incubated with free ST, ST loaded, and unloaded 3D printed scaffolds. The 1 % and 2 % (wt) ST loaded PLA/CS/ST 3D printed scaffolds showed an increase in the cell number. Annexin V/PI double stain assay was performed to test whether early or late apoptosis was induced in the PLA/CS/1 % ST and PLA/CS/2 % ST loaded groups and the results were consistent with the MTT assay. Furthermore, a wound healing assay was carried out to investigate the effect of ST loaded 3D printed scaffolds on wound healing in CCD-1072Sk cells. The highest wound closure compared to the control group was observed on cells treated with PLA/CS/1 % ST for 72 h. According to the results, novel biocompatible ST loaded 3D printed scaffolds with antimicrobial effect can be used as wound healing material for potential tissue engineering applications.

Effect of Jeevamrit on growth and flowering of iris (Iris orientalis mill) cv frigia

Effect of Jeevamrit on growth and flowering of iris (Iris orientalis mill) cv frigia

Evaluation of the heavy metal content of Sweetgum tree (Liquidambar orientalis Mill.) distributed in Mugla province

This study, it was aimed to investigate the nutritional status and heavy metal contents of Liquidambar orientalis Mill., an endemic species naturally distributed in Marmaris, Koycegiz, and Fethiye districts of Mugla province. When the soil heavy metal contents are examined, it has been determined that the Cr and Ni values are quite high in the Koycegiz and Marmaris samples. Cd, Co, Cr, Ni and Pb contents in the leaves, respectively; it was determined to be in the range of 0.04-0.09 ppm, 0.4-1.80 ppm, 2.85-6.87 ppm, 9.28-40.49 ppm and 0.52-2.15 ppm. Although some heavy metals were found to be above the limit values, no physical evidence was found that the plants in the study were adversely affected by these high values metabolically.

Electrospun Composite Nanofibers Based on Poly (ε-Caprolactone) and Styrax Liquidus (Liquidambar orientalis Miller) as a Wound Dressing: Preparation, Characterization, Biological and Cytocompatibility Results

In this study, styrax liquidus (sweet gum balsam) extracted from Liquidambar orientalis Mill. incorporated PCL fibrous scaffolds were prepared using the electrospinning method. The effects of the styrax liquidus content on the prepared scaffolds were investigated using different physico-chemical and morphological analyses. Then, the styrax-loaded nanofibers were examined for their antioxidant activity, anti-biofilm, metal chelating, antimicrobial and DNA cleavage properties. The results obtained from these studies showed that the nanofibers exhibited effective biological activity depending on the weight ratio of the styrax liquidus. In light of the data obtained from the characterization and biological studies, a sample with high ratio of balsam was built for determining the cytocompatibility analysis in vitro. The cytotoxicity studies of the selected membrane were conducted using mouse embryonic fibroblast cells. The fibrous scaffolds lead to increase the cell number as a result of high viability. According to the results, we propose a novel biocompatible electrospun hybrid scaffold with antioxidant and antimicrobial properties that can be used as wound healing material for potential tissue engineering applications.

Anti-mycobacterial activity and chemical composition of essential oils and phenolic extracts of the balsam of Liquidambar orientalis Mill. (Altingiaceae)

Liquidambar orientalis Mill. (Altingiaceae) is known in Turkish as 'sığla' or ,'günlük' due to its gum-like exudate. Its exudate, `balsam of Liquidambar? (BL), is of great commercial importance because it has pharmaceutical properties and is rich in essential oils and phenolics. In this study, we investigated the phenolic profile of L. orientalis. using HPLC, and 5 compounds from BL-1, (juglone, naringenin, rosmarinic acid, vanillic acid, echinacoside) and 4 compounds from BL-2 (juglone, apigenin-7-glucoside, naringenin, vanillic acid) were reported for the first time. A total of six new flavonoids (juglone, naringenin, rosmarinic acid, echinacoside, vanillic acid, and apigenin-7-glucoside) were identified from BL?s by HPLC analysis. According to HPLC analyses, juglone was discovered to be the major flavonoid in both samples (BL-1 and BL-2). In addition to, naringenin, rosmarinic acid (in BL-1), and apigenin-7-glucoside (in BL-2) were found in the methanol extracts of BL. The main compounds of the essential oils of BLs were (E)-ethyl cinnamate (27.5 and 30.6%), torreyol (11.0 and 12.0%), and cinnamyl alcohol (7.2 and 7.9%). We demonstrated that BLs have antimycobacterial effectiveness against four strains of M. tuberculosis using the microplate Presto Blue assay (MPBA). Each BL showed the antimycobacterial activity against four strains of M. tuberculosis. We believe that this study will contribute to phenolic profiles, essential oils, and antimycobacterial activities of the balsam of L. orientalis.

Novel styrax liquidus loaded chitosan/polyvinyl alcohol cryogels with antioxidant and antimicrobial properties

AbstractIn this study, a novel cryogel scaffold loaded with styrax liquidus gum exuded from trank of Anatolian sweetgum (Liquidambar orientalis Mill.), with antimicrobial and antioxidant properties was developed for potential tissue engineering applications. For this purpose, different ratios of styrax incorporated chitosan/polyvinyl alcohol composite scaffolds were fabricated using cryotropic gelation method. The effect of styrax incorporation onto the cryogel network was evaluated using morphological and physico‐chemical analyzes. The characterized cryogels were also investigated in detail for their antioxidant activity, anti‐biofilm, antimicrobial, and DNA cleavage properties. The styrax incorporated cryogels can be candidate biomaterials for tissue engineering applications, in particular for antimicrobial systems due to their ferrous ion chelating activity and high antioxidant, anti‐biofilm, and antimicrobial properties.

Interventions of Unani medicine for maintenance of health with special reference to air quality: an evidence-based review.

This article aims to discuss the impact of air quality on human health, measures to achieve the goal of good indoor air quality and proposed benefits of interventions of Unani Medicine with an evidence-based approach. The significance of air quality on the health of the community cannot be denied. Recent evidences from WHO illustrated data on severe air pollutants and their impacts on human health ranges from minor upper respiratory irritation to chronic respiratory ailments including lung carcinoma and heart disease associated with premature mortality and reduced life expectancy. In Unani Medicine, air has been included in the list of factors, which are six in number and play the central role in prevention of diseases and maintenance of health. Air is considered as the medium of most of the extrinsic factors such as chemical and biological pollutants affecting health and their exposure results in short and long-term health issues. The literature of Unani Medicine proposes many simple and effective measures, which help to improve indoor and outdoor air quality. The goal of outdoor clean air is achieved through implementation of measures to tackle the source of pollution, while indoor clean air is attained through various means e.g., fumigation with herbal drugs. Hence, an extensive literature survey on Unani reserve was conducted to collect information about the concept of air discussed under the heading of six essential factors and its implication in prevention of diseases and maintenance of health. Further, research databases such as Pub Med, Google Scholar, and Science-Direct were broadly searched for evidence on the efficacy of herbals mentioned in Unani literature for the indoor air purification and subsequent air quality improvement. Recent studies showed good air quality leads to decrease in mortality, particularly of respiratory and cardiovascular deaths whereas poor air quality results in a variety of diseases. Unani scholars prescribed several regimens such as Bukhoor (Fumigation), Sa'oot (Nasal instillation) and use of Abeer (Perfumes) and Nadd (Incense) for the improvement of air quality. Likewise various herbal fumigants and sprays containing drugs like mī'a sā'ila (Liquidambar orientalis Mill.), mastagi (Pistacia lentiscus L.), mushk (Moschus moschiferus L.), loban (Styrax benzoides W. G. Craib), ābnoos (Diospyros ebenum J. Koenig ex Retz), zā'fran (Crocus sativus L.) and sirka (vinegar) etc. has been well explained and used exclusively for air purification and improvement of AQI. Therefore, in the present scenario of altered air quality, we forward certain measures described in Unani system of medicine for health promotion and protection. Scientific evidence on several drugs reveal the presence of a number of pharmacologically active substances, which may provide a new approach into the purification of air.

Carpinus betulus L. (Adi Gürgen) ve Carpinus orientalis Mill. (Doğu Gürgeni) fidanlarının bazı kalite özellikleri üzerine yükseltinin etkisi

This study aims to determine some morphological characteristics of two-year-old common hornbeam (Carpinus betulus L.) and oriental hornbeam (Carpinus orientalis Mill.) seedling and to determine the quality classes of the seedlings according to Turkish Standards Institute’s (TSI) seedling quality standards and sturdiness quotient values. As a study material, two-year-old common hornbeam and oriental hornbeam seedlings, grown from seed obtained from three different altitudes as lower (0-400 m), mid (400-800 m) and upper (800-1200 m) in the Trabzon-Maçka watershed were used. The seedlings were grown in seedbeds in open nursery conditions of Karadeniz Technical University Forestry Faculty Research and Application Greenhouse. Seedling length and root collar diameter were measured in two-year-old seedlings. The sturdiness quotient was calculated with the data of the measured seedling morphological characteristics. Quality classes were classified according to TSI seedling quality standards determined for hornbeam seedlings. The highest mean seedling length for common hornbeam was determined as 48.76 cm at the 3rd altitude level, while the highest mean seedling length for oriental hornbeam was found as 38.90 cm at the 2nd altitude level. The mean root collar diameters for were varied between 4.97-5.23 mm in common hornbeam and 4.62-5.09 mm in oriental hornbeam. As a result of variance analysis, statistically significant differences (p

THE MYCOBIOTA OF SAMANLI MOUNTAINS IN TURKEY

The Mycobiota of Samanlı Mountains were investigated in this study. Specimens were collected during 3 years between November 2012 and November 2015. 510 macrofungal taxa belonging to 197 genera within 84 families were recorded with field and laboratory studies. Of these, 37 genera and 57 taxa belong to Ascomycota, while 160 genera and 453 taxa belong to Basidiomycota. Nine species were found for the first time in Turkey from Basidiomycota. These taxa are Amanita subnudipes (Romagn.) Tulloss, Hebeloma quercetorum Quadr., Hygrocybe obrussea (Fr.) Wunsche, Lactarius mediterraneensis Llistosella & Bellù, Lactifluus glaucescens (Crossl.) Verbeken, Russula lilacea Quél., R. rubra (Lam.) Fr., Stereopsis reidii Losi & A. Gennari and Tricholoma roseoacerbum A. Riva. The Stereopsidaceae family and the genus Stereopsis D.A. Reid was found for the first time in Turkey.The richest families in terms of the number of taxa are Russulacea with 58 taxa (11.3%), Agaricaceae with 46 taxa (8.9%), Tricholomataceae with 43 taxa (8.4%), Boletaceae with 32 taxa (6.2%), Polyporaceae with 23 taxa (4.5%) and the most crowded genera are Russula Pers. with 41 taxa (8%), Tricholoma (Fr.) Staude with 26 taxa (5%), Amanita Dill. ex Boehm. with 19 taxa (3.7%), Lactarius Pers. with 16 taxa (3.1%) and Inocybe (Fr.) Fr. with 14 taxa (2.7%). The ecological status of the species is as follows; 245 (48%) are saprobe, 226 (45%) are mycorrhizal, 20 (3.7%) are lignicolous, 18 (2.9%) are parasite, and one is entomopathogenic. Habitat distribution in the research area is as follows: 300 species in Abies nordmanniana (Stev.) Spach. subsp. bornmuelleriana (Mattf.) Coode & Cullen forest, 295 species in Fagus orientalis Lipsky forest, 125 species in Quercus spp. forest, 88 species in Pinus nigra J.F.Arnold forest, 56 species in Castanea sativa Mill. forest, 53 species in Pinus sylvestris L. forest, 49 species in Carpinus orientalis Mill. forest and 24 species in Pinus maritima Lam. forest.

The Phytochemical Profile and Biological Activity of Liquidambar orientalis Mill. var. orientalis via NF-κB and Apoptotic Pathways in Human Colorectal Cancer

Liquidambar orientalis Mill. var. orientalis (Hammamelidaceae) commonly known as oriental sweet gum is a medicinal plant endemic to Turkey, and used for treatment of wounds such as ulcers, gastritis and skin diseases. However, there are only a limited number of studies on the anticancer properties of this plant. The aim of this study is to investigate the cellular and molecular mechanisms of L. orientalis in colorectal cancer cell lines and to reveal the active therapeutic components. Antioxidant, anti-proliferative, anti-invasive and apoptotic effects were investigated for determining biological activities. The phytochemical profile and quantitation assays were carried out by using HPLC-ESI-Q-TOF-MS. The results demonstrated that leaf methanol extract (LM) of L. orientalis has the highest cytotoxic activity in HCT-116 (IC50 27.80 μg/mL) and HT-29 (IC50 43.13 μg/mL) cell lines as compared to the other extracts tested. Regarding chemical composition, quercetin 3-glucoside (61.005 ± 1.527 mg/g extract), chlorogenic acid (31.627 ± 0.970 mg/g extract), pyrogallol (9.950 ± 0.001 mg/g extract), epigallocatechin gallate (9.671 ± 0.004 mg/g extract), apigenin 7-O-glucoside (2.687 ± 0.027 mg/g extract), gallic acid (2.137 ± 0.012 mg/g extract), genistin (1.270 ± 0.027 mg/g extract), luteolin (0.055 ± 0.0005 mg/g extract) and kaempferol (0.506 ± 0.007 mg/g extract) were identified as the major phytochemical compounds. Our results showed that LM extract exhibited In Vitro cytotoxic and apoptotic properties.

Relative Susceptibility, Preference, and Suitability ofCarpinusTaxa for the Japanese Beetle (Coleoptera: Scarabaeidae) 1

AbstractNineteen Carpinus taxa were evaluated in no-choice and multiple-choice laboratory feeding bioassays for susceptibility, preference, and suitability for the adult Japanese beetle (Popillae japonica Newman). No-choice laboratory feeding bioassays revealed that only three Carpinus taxa, Carpinus caucasica Grossh., Carpinus tschonoskii Maxim., and the hybrid Carpinus caroliniana x C. coreana, were significantly less susceptible and less suitable to feeding and for reproduction by Japanese beetles. Leaf tissue removed was related to the amount of frass produced, but was not related to leaf thickness, inner or outer leaf toughness, or fecundity. Frass production did not reflect fecundity. Hybridization may have either a positive or negative effect on host susceptibility, preference, and suitability. When a moderately preferred species such as C. caroliniana Walter or C. betulus L. is crossed with a species of equal or higher susceptibility, (i.e. C. coreana Nakai, Blume, or C. laxiflora (Sieb. and Zucc.) Blume) feeding preference increases. A “C. tschonskii factor” (Maxim.) appears to affect susceptibility and preference. There does not appear to be a large pool of Carpinus taxa suitable for future tree breeding programs in areas where Japanese beetle outbreaks are common. Potential use of Carpinus taxa in urban landscapes and forests is discussed.Index words: Susceptibility, preference, suitability, Japanese beetle, Popillia japonica, Carpinus.Species used in this study: Carpinus betulus L., C. caroliniana Walter, C. caucasica Grossh, C. cordata Blume, C. coreana Nakai, C. fargesii Franch, C. japonica Blume, C. laxiflora, (Sieb. and Zucc.) Blume, C. orientalis Mill, C. tschonoskii Maxim, C. turczaninowii Hance, C. betulus x tschonoskii, C. caroliniana x betulus, C. caroliniana x (C. betulus x tschonoskii), C. caroliniana x cordata, C. caroliniana x coreana, C. caroliniana x orientalis, C. caroliniana x tschonoskii, C. caroliniana x coreana, C. cordata x C. japonica, and, Sassafras albidum (Nutt.) Nees.

Liquidambar orientalis Mill. gum extract induces autophagy via PI3K/Akt/mTOR signaling pathway in prostate cancer cells

ABSTRACT Liquidambar orientalis Mill (LOM), is an endemic species having a local distribution in the southwestern coastal district of Turkey. Styrax liquidus gum (SLG), is a gum-like resinous which exudates in response to injury of the trunk of LOM. The aim of the study was to investigate the cytotoxic effects and the molecular mechanisms of the ethanolic SLG extract in human prostate cancer cells. GC-MS analysis was performed to identify the volatile compound composition. Cytotoxicity was determined by XTT analysis. Apoptosis and necrosis were evaluated via ELISA assay. Autophagic cell death was detected via monodansylcadaverine (MDC) staining and by measuring the levels of LC3I and LC3II. The protein levels of p-PI3K, p-Akt and p-mTOR were evaluated by western blot analysis. In the present study, it is shown that the SLG extract containing a considerable amount of ravidomycin derivate induced autophagic cell death in prostate cancer cells via inhibiting the PI3K/Akt/mTOR pathway.

Su Tutucu Polimer (SAP) Uygulamalarının Anadolu Sığla Ağacı (Liquidambar orientalis MİLL.) ve Biberiye (Rosmarinus officinalis L.) Türlerinde Bitki Gelişimi Üzerine Etkisi

Küresel ısınmadan kaynaklanan iklim değişimi ve hızlı nüfus artışına bağlı olarak tüketimin yükselmesi doğal kaynaklardan suyun azalmasına neden olarak varlığını tehdit etmektedir. Dünyada kullanılabilir suyun büyük bir bölümü tarımda değerlendirildiğinden bitkilerin su ihtiyacının en doğru ve ekonomik şeklide karşılanması gerekmektedir. Bu bağlamda da suyun verimli ve ekonomik kullanılmasını sağlayan yöntemlerden biri olarak su tutucu polimer (SAP) uygulaması karşımıza çıkmaktadır. Araştırmada, su tutucu polimer SAP A200‘ün farklı dozları [% 0 (kontrol), % 0,2, % 0,4 ve % 0,6 v/v] geleneksel (toprak) ve topraksız tarım yetiştirme ortamlarına [torf, perlit ve torf:perlit (1:1) v/v] uygulanmıştır. SAP A200 dozlarının Anadolu Sığla Ağacı (Liquidambar orientalis Mill.) ve Biberiye (Rosmarinus officinalis L.) türlerinin bitki gelişimine etkisinin belirlemesi amaçlanmıştır. Araştırma tesadüf parselleri deneme desenine göre 3 tekerrürlü olarak kurulmuştur. Bitki gelişim özelliklerini belirlemek amacıyla bitki boyu, gövde çapı, kök uzunluğu, kök kalınlığı, kök ile sürgün yaş ve kuru ağırlıkları belirlenmiştir. Araştırmadaki iki tür için, tüm SAP dozlarının bitki gelişim parametrelerini olumlu yönde etkilediği ve en yüksek değerlerin % 0,6 SAP A200 uygulamasında tespit edildiği gözlemlenmiştir. Toprak dışındaki tüm yetiştirme ortamlarında bitki gelişim değerlerinin daha yüksek olduğu ve en iyi sonuçların torf:perlit ortamında gerçekleştiği saptanmıştır.

Phenotypic characterization of Prunus haussknechtii Bornm., P. elaeagnifolia Spach, and P. orientalis Mill.

Wild almond related species (subgenus Amygdalus in Prunus genus, Rosaceae family) are considered as valuable genetic resources which can be used to broaden the genetic background and to introduce new traits into commercial relatives. The current research was conducted to describe the phenotypic variability of three wild almond related species including Prunus haussknechtii Bornm., P. elaeagnifolia Spach, and P. orientalis Mill. from natural habitats. There were considerable variations among the accessions studied of each species. The range of nut weight was 0.44–1.80 g in P. haussknechtii, 0.24-0.78 g in P. elaeagnifolia, and 0.25-0.99 g in P. orientalis. Principal component analysis (PCA) indicated 17 principal components (PCs) with explaining 79.58% of total variance in P. haussknechtii, 14 PCs with explaining 89.54% of total variance in P. elaeagnifolia, and also 14 PCs with explaining 89.45% of total variance in P. orientalis. The generated dendrogram based on the combined data obtained from the three species studied showed two different major clusters and indicated high morphological diversity among and within the studied species. Results showed that the studied wild species are suitable candidates for breeding objectives of almond, such as developing rootstocks with resistance to drought and also developing late-flowering cultivars. The current investigation provided basic genetic information and a reliable basis that should facilitate attempts to protect the species studied for in situ conservation.